Title of Invention

"PROCESS FOR PREPARING AN OIL CONTAINING ONE OR MORE LONG-CHAIN POLYUNSATURATED FATTY ACIDS DERIVED FROM BIOMASS; FOODSTUFF OR NUTRITIONAL, COSMETIC OR PHARMACEUTICAL COMPOSITION CONTAINING IT"

Abstract A stable oil containing LC-PUFAs in the form of triacylglycerol, in particular arachidonic acid (ARA), dihomogammalinolenic acid (DHGLA), docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA), may be prepared by direct pressing of one or more biomasses obtained from the culture of a microorganism, especially of a fungus or of a microalga containing the acids ARA, DHGLA, DHA or EPA leading to a first press oil and by bringing a carrier oil entering into the composition of a foodstuff, a cosmetic or pharmaceutical product, into contact with the biomass cake, followed by pressing leading to a second press oil, and then by combining the pressed oils and refining the mixture under controlled conditions.
Full Text Process for preparing axx oil containing one or more long-chain polyunsaturatad fatty acids derived from biomass; foodstuff or nutritional, cosmetic or pharmaceutical composition containing it
Introduction
The present invention relateH to the field of the preparation of an oil serving as an ingredient which is a source of long-chain polyunsatura^d essential fatty acids (LC-PUFAs) in a foodstuff, in a nutritional supplement, in a cosmetic or pharmaceutical composition.
State of the art
An oil containing LC-PUFAs such as for example arachidonic acid (ARA), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) or dihomogammalinolenic acid (DHGLA) may be obtained from a biomass fermentation broth. To obtain the oil from the biomass, methods of extraction with organic solvent, for example hexane, or with supercritical fluid, have been used. Generally, the oil has been extracted from biomass by percolation of the dried biomass with hexane.
Such a process of extraction with organic solvent(s) is described, for example, in WO9737032, in WO 9743362 or in the publication Journal of Dispersion Science and Technology, 10, 561-579, 1989 "Biotechnological processes for the production of PUFAs".
This technique has various disadvantages:
During the stages of extreiction with hot solvent or of distillation of the solvent, the LC-PUFAs may undergo degradation in contact with oxygen.
The complete removal of the solvent contained in the oil or in the residual biomass requires a
heat treatment at high temperature.
Moreover, the solvent, such as hexane, is capable of dissolving nontriacylglycerol constituents of the biomass which in fact constitute impurities.
The crude oil obtained after evaporation of the solvent should further undergo several refining stages comprising degumming, awuLraiization with alkali, decolorization, dewaxing and deodorization with the aim of at least partially removing the impurities. This means that the highly unsaturated oil is exposed to conditions stimulating physicochemical reactions which affect its quality. For example, the decolorization agents create a system of conjugated double bonds and form degradation products by chemical reaction with the oxidized glycerides.

A process for extracting biomass with no solvent is also known, for example from EP-A-1178118. According to this process, the use of solvent is avoided by preparing an aqueous suspension of the biomass and by separating an oily phase, containing the desired oil, from the acpjeous phase, by centrifugation. The aqueous phase contains cell wall d«brle and a quantity of water-soluble material obtained from the biomass. A disadvantage of this process is that the crude oil obtained is contaminated with quantities of impurities, for example polar lipids, residues of proteins due to the presence of water. Such a crude oil should then be refined by conventional methods for refining vegetable oils.
The aim of the present invention is to avoid the disadvantages of the prior art, by providing a process for preparing a stable oil containing one or more polyunsaturated fatty acids derived from biomass in the form of triacylglycerols in the purified state with a high yield and in which the oil undergoes minimum
degradation.
Summary of the invention
The present invention relates to a process for preparing a stable oil containing LC-PUFAs in the form of triacylglycerols, in particular arachidonic acid
(ARA), dihomogammalinolenic acid (DHGLA), docosahexaenoic acid (DHA) or eicosapentaenoic acid
(EPA).
The process is characterized in that one or more biomasses obtained from the culture of a microorganism, especially of a fungus or of a microalga containing the acids ARA, DHGLA, DHA or EPA, are pressed in the dry state so as to obtain a first press oil and a cake, and in that the oil thus obtained is treated with an adsorbent and in that it is subjected to deodorization under controlled conditions.
The oil thus obtained does not require chemical refining. Treatment with an adsorbent, for example a silicate, and deodorization under gentle conditions are sufficient to obtain a purified and stable oil which can be used as a nutritional supplement.
According to one preferred embodiment of the process/
the press cake is placed in contract with a carrier oil entering into the composition of a foodstuff and the mixture of the carrier oil and the cake is pressed in order to obtain a second press oil, and the pressed oils are mixed in variable proportions so as to obtain an LC-PUFA concentration which is appropriate for the specific application. The mixture is then subjected, where appropriate, to physical refining and to deodorization.
Preferably, the oil does not contain more than 10% by weight of LC-PUFAs. As a result, the oil is a lot less
sensitive to oxidation during its production, which is not the case for the oils containing LC-PUFAs of the prior art.
According to a main aspect of the invention, it is a crucial qualitative advantage to have available a novel oil containing LC-PUFAs in the form of triacylglycerols.
According to another aspect, the invention relates to a foodstuff, a cosmetic or pharmaceutrical product, a nutritional supplement or an animal feed containing the preceding oil.
According to yet another aspect, the invention relates to an animal feed, in particular for pets, containing the biomass residue derived from the process.
Detailed description of the invention
The preparation of the oil is carried out by direct pressing of a biomass containing LC-PUFAs and production of a first press oil. In order to increase the LC-PUFA yield, the biomass cake obtained from the direct pressing is brought into oontact with a carrier oil and the mixture is subjected to pressing in order to obtain a second press oil. The two/pressed oils are then mixed in variable proportions so as to obtain an LC-PUFA concentration which is appropriate for the specific application. The mixture is then subjected, where appropriate, to physical refining and the desired oil is thus obtained.
Physical refining, in the context of the invention, is aimed at a reduction in the phospholipids and the free fatty acids and is understood to mean a deguraming treatment without the use of acid and without neutralization.
By way of example, it was observed that in the case of a biomass containing ARA, the first press oil was already free of most free fatty acids and phospholipids and, consequently, depending on the desired degree of purity, did not require degumming treatment.
On the other hand, in the case of a biomass containing DHA for example, such a degumming treatment was necessary in order to reduce, in particular, the phospholipids so as to obtain the desired degree of purity.
The oil obtained is suitable for application in foodstuffs, in particular infant formulas or for use as a nutritional supplement. It may also be used in cosmetic or pharmaceutical products. Furthermore, the biomass residue obtained is also a product of the process which may be upgraded directly without subsequent treatment, for example as animal feed, in particular for pets.
The preparation of such an oil may take place by simply pressing the dried biomass. Preferably, the carrier oil is mixed with the residue of the first pressing of the biomass and the oil iti subsaquantly separated from the nonlipid solids by pressing.
In order to increase the yield of LC-PUFA obtained, it is preferable to reduce the sizes of the particles of dry biomass in order to break the walls of the cells of microorganisms and to thereby release the oil. It is possible, in the preferred embodiment, to reduce the sizes of the particles of the press cake in order to increase the surface area of contact between the carrier oil and the biomass residue. This may be appropriately carried out using various methods, for example:
the biomass cake may be ground in the presence of the carrier oil;
the biomass cake may be laminated before mixing it with the carrier oil;
the biomass cake may be treated at high pressure in the presence of the carrier oil, and then the oil obtained may be separated from the biomass cake by pressing and final filtration;
the biomass or cake may be treated with enzymes capable of degrading tha wails of the cells.
Because the carrier is an oil, the oil obtained after contact with the biomass cake has a minimum content of phospholipids, free fatty acids, pigments, polymers and other substances obtained or derived from the biomass which are not triacylglycerols. This means that the process according to the invention constitutes a selective method for preparing a stable purified oil containing LC-PUPAs. It is not necessary to purify the unsaturated oil containing the LC-PUFAs by the aggressive and cumbersome methods used prior to the invention such as the stages of degumming, neutralization, dewaxing and decolorization.
According to the invention, the pressed oils are subjected, where appropriats, l-u a refining step using a processing agent, for example ci silicate. The treatment with the processing agent may be carried out during contact with the carrier oil or after production of the pressed oil, for example during filtration. Finally, the oils are subjected to a stage of deodorization, for example by steam distillation or molecular distillation at a relatively low temperature. The result is that the oil obtained contains a particularly small quantity of trans fatty acids.
The process does not use organic solvent and, since the operation is carried out under a nitrogen layer and in the presence of tocopherols or tocotrienols which are naturally present in or which are added to the carrier oil, the LC-PUFAs are protected from oxidative
degradation during the whole process.
In addition to the quality of the oil obtained, another advantage of the process consists in the fact that the biomass residue is not contamlnated with an organic solvent and may thus be dir«ctiy upgraded, without subsequent treatment, for example in/ animal feed, in particular for pets.
The detailed description of the process which follows is targeted at the preparation of an oil containing ARA, an oil containing DHA, and an oil containing ARA and DHA, taken by way of nonlimiting exan^les. The working conditions for transferring other LC-PUFAs to a carrier oil from appropriate biomasses, for example for DHGLA or EPA, are very similar.
In the preferred implementation of the process, the oil is obtained by combining the biomass dry-pressing oil and the oil obtained by mixing the carrier oil with the dry biomass pressing residue and separating the oil from the solid components by pressing. To increase the level of incorporation of LC-PUFA, it is desirable to break the microbial cells by high-pl-essure treatments, by enzymatic processes, or to reduce the sizes of the dry particles of biomass by grinding or laminating.
The grinding step used may be one of many techniques known in the prior art. For example, the biomass may be laminated, preferably at low temperature, and then it may be mixed with the carrier oil. As a variant, the biomass may be ground in the presence of the carrier oil. In order to minimize as much as possible damage to the LC-PUFAs, the grinding conditions should be gentle. In this regard, grinding the biomass in the presence of the carrier oil and under an inert atmosphere, for example under a nitrogen stream, is preferred.
Next, the oil containing the LC-PUFAs is separated from
the biomass cake by filtration or pressing, preferably
at high pressure, and then a final filtration is
carried out so as to remove the fine particles of
biomass residue.
It was observed that the level of incorporation of the LC-PUFA increased when the size of the biomass particles decreased; it was > 90% when for example 90% of the particles had a size By way of example, it is possible to use a ball mill or a colloidal mill. The parameters to be considered are the duration of grinding, the size of the biomass particles, the grinding temperature, the ratio between the quantities of biomass and of carrier oil.
The duration of grinding has an influence on the size of the particles and the latter is also influenced by the grinding temperature. Consequently, in practice, it is preferable to indicate the size of the particles as a crucial parameter of the grinding stage. Thus, it is desirable that 90% of t hn particles have a size The grinding temperature is chosen at a value greater than the melting point of the carrier oil, and is preferably from 20 to 80°C.
The weight ratio chosen between the biomass and the carrier oil determines the content of LC-PUFA of the final oil. Thus, for example, 30 parts of biomass are chosen per 70 parts of carrier oil in order to obtain at least 3.5% of LC-PUPA in the converted oil.
The oil used as carrier may be any oil or mixture of oils which can be consumed as human food. An oil or a mixture entering into the composition of the product
which it is desired to enrich with PUFA is preferably used. There may be mentioned in particular for an infant formula high oleic acid sunflower oil (HOSFO), sunflower oil (SFO) , soya bean oil, palm olein and a medium-chain triacylglycerol (MCT, containing essentially triacylglycerols of saturated C8-C10 fatty acids).
The next stage of the process consists in separating the spent biomass resldue by any customary method such as, for example, pressing, filtration or centrifugation. To this end, a presss operating at high pressure is preferably used.
The oil obtained should be made free of fine insoluble particles by fine filtration. This operation may be carried out, where appropriate, by exposing the oil to a mineral adsorbent as a filter aid, for example dicalite.
Finally, the filtered oil is deodorized in order to remove the volatile substances. This may be carried out by any known method provided that moderate conditions are used in order to be gentle on the LC-PUFAs. There may be mentioned, for example, steam distillation, preferably under vacuum, or molecular distillation.
The oil obtained may be used in fopd compositions for human consumption as it is or in the form of a mixture with other oils such as, for example, a fish oil or such as, for example, salad oils or alternatively in the form of an emulsion in salad dressings or mayonnaises. It may be a constituent of a dietetic milk for teenagers or adults, an infant formula for premature babies, full-term unweaned babies or a follow-on milk for small children.
It may be incorporated into a nutritive or supplemental composition for oral consumption.
It may be incorporated into a pharmaceutical composition for oral, enteral or parenteral ingestion, or for topical, dermatological or ophthalmological application.
It may constitute an ingredient for a cosmetic, topical or oral composition.
Finally, it may constitute an ingredient for a pet food, for example a dry or moist food or a milk.
The biomass residue, after separation'of the oil, may be advantageously used in animal feed, particularly for pets.
Examples
The examples below illustrate the invention. Therein,
the parts and percentages are by weight, unless
otherwise stated.
To determine the % of free fatty acids (FFA), the lUPAC
2.201 method is used,
for the ARA content, the lUPAC 2.304 method is used,
and
for the Phosphorus (P) content, the NI C12-1976-SSOa
method is used,
Example 1; Pressing of a biomass conjiaining ARA with a
hydraulic press
Equipment:
Carver RC 106 press
Materials used;
Biomass containing 34.7% of oil containing 39.9% of
arachidonic acid (ARA)
Procedure:
111 g of the biomaas are introduced into a carton
cartridge. The cartridge is introduced into the
cylinder of the press and the whole is heated in an
oven at 70°C for 3 0 min.
The pressing is then carried out by gradually
increasing the pressure up to a final pressure of
700 bar. 23.2 g of clear pressed oil are thus
recovered, which corresponds to 60.2% of oil present in
the biomass.
The ARA, free fatty acid (FFA) and Phosphorus (P)
oontont of the pressed oil is then determined and It is
compared with an oil obtained after hexane extraction
of the same biomass. The results are summarized in
Table 1 below.
Table 1

(Table Removed)
The oil obtained after direct pressing has an ARA content similar to the oil extracted with hexane but contains less impurities (FFA, P) . Tlie pressed oil does not req[uire chemical refining unlike the oil conventionally extracted with hexane. A treatment with an adsorbent (processing agent) followed by deodorization make it possible to obtain a stable oil having a neutral odour.
Example 2: Pressing of a biomaas containing DHA with a
screw press
Equipment :
Komet screw press
Seitz filter press
Materials used;
Biomass containing 50.5% of oil containing 39% of
docosahexaenoic acid (DHA)
Procedure;
150 kg of biomass were pressed in the Komet press under
the following conditions:
slit: 8 mm, throughput: 3.75 kg/h A cloudy oil is collected which is filtered using a 20 micron paper filter. 46 kg of a clear oil are thus obtained, which correeponda to 60.7% of oil present in the biomass.
The DHA, free fatty acids (PPA) and Phosphorus (P) content of the pressed oil is determined and it is compared with an oil obtained after extracting the same biomass with hexane. The results are summarized in Table 2 below.

(Table Removed)
The oil obtained after direct pressing has a DHA content similar to the oil extracted with hexane but contains less impurities (FFA, P) . The losses during refining are lower. Treatment with an adsorbent (processing agent) followed by deodorization make it possible to obtain a stable oil having a neutral odour.
Example 3; Incorporation of the residual DHA of the
pressed biomass into palm olein
Equipment;
Fryma MZ 80 colloid mill
De Smet minipilot filuration unit with 1.4 1 chamber
Seitz 2 1 filter press
Laboratory deodorizer
Materials used;
Pressed DHA biomasa of Example 2 containing 28.2% of
oil containing 3 9% of decosahexaenoic acid (DHA)
Palm olein
Trisyl® (processing agent)
1.4 kg of the presaed biomass as obtained in Example 2 and 2 kg of palm olein are intruduced into the container of the mill. The milling is carried out by direct passage through the mill with a slit of 10 microns at a temperature of 40-50°C and the mixture is recovered. 2.6 kg of this mixture are introduced into the filtration unit and filtration is carried out at 50°C, and then the cake is washed by injecting 0.5 kg of palm olein. The cake is then pressed up to a pressure of 15 bar. 2 kg of a slightly cloudy oil are thus recovered, which oil is filtered on the filter press with a 1 micron filter. The P content of the filtered oil, which is up to 66 mg/kg, is determined. The filtered oil is subjected to physical refining. -With the aim of reducing the phosphorus content, the oil is stirred at- 85°C for 20 min with 2% Triayl®, and then dried at 85°C under a 20 mbar vacuum and filtered. Finally, the oil is deodorized at 180°C/1 mbar for 3 hours. The refined oil is analysed in order to determine its purity (FFA, P) and the level of incorporation of the DHA. The results are summarized in Table 3 below.

(Table Removed)
Example 4: Physical refining of the mixture of pressed oils containing DHA
Equipment :
1 1 laboratory glass readlot Seitz 2 1 filter press Laboratory deodorizer
Materials used;
Trisyl® (processing agent)
Procedure:
With the aim of obtaining a refined oil with a DHA concentration of about 8%, 50 g of pressed oil as obtained in Example 2 and 500 g of pressed oil as obtained in Example 3 before the refining step are mixed in the reactor. The mixture is stirred at 85°C for 20 min with 2% of Trisyl®, and then dried at 85°C under a 20 mbar vacuum, and finally filtered. 540 g of a clear oil are thus recoveired.
liOO g of this oil are introduced into the deodorizer and treated with steam for 3 hours at 180°C/1 mbar. The refined oil is analysed in order fb determine its purity (FFA, P) and the DHA content. The results are summarized in Table 4 below.
Table 4
(Table Removed)

Example 5 : Incorpration. into palm olain. of DHA and
ARA obtained from biomass
Equipment :
Fryma MZ 80 colloid mill
De Smet minipilot filtration unit with 1.4 1 chamber
Seitz 2 1 filter press
Laboratory deodorizer
Materials used.-
Biomaes containing 36% of oil containing 43.1% of
arachidonic acid (ARA)
Biomass containing 50.5% ot oil containing 39% of
decoeahexaenoic acid (DHA)
Palm olein
Trisyl® (processing agent)
Procedure:
0.7 )kg of the biomasa containing DHA, 0.7 kg of the biomass containing ARA and 2 kg of palm olein are introduced into the container of the mill. The milling is carried out by direct passage through the mill with a slit of 10 microns at a temperature of 40-50°C and the mixture is recovered. 2.5 kg of this mixture are introduced into the filtration unit and filtration is carried out at 50°C. The cake is washed by injecting 0.92kg of palm olein. The cake is then pressed up to a pressure of 15 bar. 2.B kg of a slightly cloudy oil are thus recovered, which oil is filtered on the filter press with a 1 micron filter. The P content of the filtered oil, which is up to 26 mg/kg, is determined. The filtered oil is subjected to physical refining. With the aim of reducing the phosphorus content, the oil is stirred at 85°C for 20 min with 1% Trisyl®, dried at 85°C under a 20 ml)ar vacuum and then filtered. Finally, the oil is deodorized at 180°C/1 mbar for 3 hours. The refined oil is analysed in order to determine its purity (FPA, P) and the level of
incorporation of the DHA and of the ARA. The results are summarized in Table 5 below.

(Table Removed)
Examples 6-8
6. An infant formula for premature babies enriched with ARA is prepared from oil prepared by the process of Example 1 and a fish oil enriched with DHA, containing about 24% of DHA and there are added thereto other oils, for example in the proportions indicated in Table. 6 below, proteins, where appropriate hydrolysed, carbohydrates, and where appropriate vitamins and trace elements.

(Table Removed)
7. An infant formula for fuli-term unweaned babies enriched with ARA and with DHA is prepared from the carrier oil prepared by the process of Example 5 and
there are added thereto other oils, for example in the proportions indicated in Table 7 below, proteins, where appropriate hydrolysod, carbohydrates and where appropriate vitamins and trace elements.
Table 7

(Table Removed)
8. A follow-on milk for small children enriched with DHA is prepared from the carrier oil prepared by the process of Example 4, and there are added thereto other oils in the proportions indicated in Table 8 below, proteins, where appropriate hydrolysed, carbohydrates and where appropriate vitamins and trace elements.
Table 8

(Table Removed)
A liquid milk enriched with DHA in an amount of 1% of DHA in the fatty phase is prepared in the following manner:
A whole milk containing 3.92% of fat and 8.58% of solids-not-fat and a low-fat milk containing 0.05% of fat and 9% of solids-not-fat are pasteurized separately
by treating them at 87°C for 12 s.
34.69 kg of whole milk and 160.26 kg of low-fat milk, cooled to 15°C, are then mixed, and then a premix of 0.77 kg of oil obtained according to Example 3 (palm olein, containing 4.9% of DHA) , 1.6 kg of soyabean oil and 1 g of vitamin E heated to 50°c is incorporated into this mixture by means of a colloid mill.
Sterilized product :
After heating to 80°C. In n |)lat;o oxchanger, the liquid is UHT ateriiized at 148°C for , a, After cooling at 78°C, it is homogenized in two stages, at 200 bar, and then at 50 bar; it is cooled to 20°C and it is aseptically packaged in carton-type packaging which has been previously sterilized, the homogenization, cooling and filling stages taking place aseptically.
Pasteurized product:.
The liquid is heated at 72 °C for 15 s in a plate exchanger; it is homogenized in two stages at 200 bar, and then at 50 bar; it is cooled to 4°C and it is packaged in carton-type packaging.
Example 1.0
As a nutritional supplement, an oil prepared according to Example 1 containing AHA or an oil prepared according to Example 2 containing DHA is encapsulated in an amount of 500 mg of oil in gelatin capsules.



We claim:
1. A process for preparing a stable oil containing long chain poly unsaturated fatty acids (LC-PUFAs) in the form of triacylglycerols from one or more biomass obtained from the cultures of a microorganisms especially of a fungus or of a microalga containing arachidonic acid (ARA), dihomogammalinolenic acid (DHGLA), docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA), are pressed in a dry state so as to obtain a first press oil and pressed cake. Said pressed cake is placed in contact with carrier oil and the mixture of the carrier oil and the cake is pressed to obtain second press oil. The mixtures of first and second press oil are combined and are treated with an adsorbent and that is subjected to deodorization at 180°C /1 mbar for 3 hours, under inert atmosphere.
2. The process as claimed in claim 1 wherein the biomass contains at least one long-chain polyunsaturated fatty acid chosen from ARA and DHA.
3. The process as claimed in claim 2, wherein said biomass contains ARA is treated.
4. The process as claimed in claim 2, wherein said biomass containing DHA is treated.
5. The process as claimed in claim 2 wherein said biomass is a mixture of biomass, containing ARA and DHA.
6. The stable oil claimed in claim 1 is suitable for food stuffs, animal feed, cosmetic applications.

Documents:


Patent Number 243016
Indian Patent Application Number 1174/DELNP/2005
PG Journal Number 40/2010
Publication Date 01-Oct-2010
Grant Date 23-Sep-2010
Date of Filing 24-Mar-2005
Name of Patentee NESTEC S.A.
Applicant Address AVENUE NESTLE 55, CH-1800, VEVEY, SWITZERLAND.
Inventors:
# Inventor's Name Inventor's Address
1 BERTHOLET, RAYMOND CH, VERS-CHEZ-COCHARD 11, CH-1807 BLONAY, SWITZERLAND.
2 WANG, JUNKUAN CHEMIN DES ABBESSES 2B, CH-1027 LONAY, SWITZERLAND
3 WATZKE, HERIBERT, JOHANN (J) CH.DE LA FONTANNETAZ 21, CH-1009 PULLY, SWITZERLAND
4 GERMAN, JOHN, BRUCE RESIDENCE LA BERGERIE, CH-1072 FOREL/LAVAUX, SWITZERLAND
PCT International Classification Number C11B 1/10
PCT International Application Number PCT/EP2003/008745
PCT International Filing date 2003-08-07
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 PCT/EP2003/008745 2003-08-07 PCT