|Title of Invention||
"METHOD FOR PREPARING A FORMULATION CONTAINING A MICRODOSED ACTIVE PRINCIPLE"
|Abstract||The method for preparing a formulation containing microdosed calcitriol, the said method being characterised by the following preparation steps: 1. weighing calcitriol in a container lined with petroleum jelly; 2. encapsulating the calcitriol with additional petroleum jelly; 3. introducing the container holding the calcitriol and the petroleum jelly into a mixer containing a mixture of excipients; and 4. diffusing the calcitriol in the mixer.|
|Full Text||The present invention relates to a method for preparing a pharmaceutical or cosmetic formulation, preferably an ointment, containing an agent or a micro-dosed disperslble active principle, comprising a plurality of preparation steps:
1. weighing the agent or active principle in a container lined with petroleum
2. encapsulating the agent or active principle with additional petroleum
3. introducing, the container holding the agent or active principle and the
petroleum jelly into a mixer; and
4. diffusing the agent or active principle in the mixer.
It is known that the manufacture of preparations with a low active principle concentration entails problems of homogenising the active principle in its excipi-ents. This is because microdosed active principles encounter dispersion heterogeneity problems when they are mixed with large volumes of excipients. These preparations normally need to undergo an intermediate diffusion phase in order to obtain products with a low active principle concentration. An additional problem arises when the active principle belongs to a class of toxic products. In this case, the handling not only takes a long time but also becomes dangerous, which significantly increases the risks of accident. Added to this is the problem of transporting the toxic product while keeping it protected it from light and oxidation phenomena.
One solution to this problem is a novel method according to the invention for preparing a formulation containing an active principle, characterised in that it-comprises the following preparation steps:
1. weighing an active principle in a container lined with petroleum jelly;
2, encapsulating the active principle with additional petroleum jelly;
3. introducing the container holding the active principle and the petroleum
jelly into a mixer; and
4, diffusing the active principle in the mixer.
These various steps will make it possible to save time and improve quality with a view to obtaining a homogeneous product.
This is because the method according to the invention provides a diffusion constant, in respect of the active principle diffusing from the container into the mixer, which avoids the heterogeneous dispersion observed when it is introduced "loose" into the vat of the mixer, as may have been carried out in the s
Encapsulating the active principle in a petroleum jelly cocoon furthermore obviates the steps of diluting an active principle, and circumvents the risks of toxicity and accidents whe.n handling the active principle after weighing, in particular
while it is being transported to the vat.
Lastly, owing to the protection provided by the petroleum jelly encapsulation, the leaktightness created by the method according to the invention avoids the problems of oxidation and degradation by light.
By eliminating the intermediate dilution steps, therefore, this invention will also
shorten the preparation time.
Ointments are preparations for external use, intended to be applied directly on
If the active principle in the ointment is a solid, it will be sprinkled as finely as
possible and incorporated into the ointment by the principle of geometrical dilution.
Geometrical dilution involves a series of dilution steps. It starts with the active principle being incorporated into an amount of excipient approximately of equal size.
A second amount of excipient, approximately equal to the first mixture that has been formed, is added and then mixed.
This procedure with dilution steps is carried out until all of the excipient has been used, so as to obtain the intended concentration of active principle. The various products involved in the manufacture of the ointment are generally mixed together by melting over a water bath, followed by stirring until the mixture has cooled. in this case, the active principle will be incorporated at a suitable stage.
It is this iterative process which is essential for producing a homogeneous ointment.
General Description of the Invention;
The Invention relates to a method for preparing a formulation, preferably an ointment, containing a microdosed active principle. The said method is characterised by the following preparation steps:
1. weighing an active principle in a container lined with petroleum jelly;
2. encapsulating tire active principle with additional petroleum jelly;
3. introducing the container holding the active principle and the petroleum
jelly into a mixer; and,
4. diffusing the active principle in the mixer.
In particular, a homogeneous liquid formulation containing a microdosed active
principle is prepared as follows:
- During step 1, the weight of the container will first be measured (see Fig. 1a)
;before being provided with a layer of white petroleum jelly at the bottom (see Fig. 1b).
Weighing will then need to be carried out in order to determine the weight of petroleum jelly which has been added. The active principle will then be Introduced into the container, as indicated in Fig. 1c. The container will then be re-weighed in order to determine the exact amount of active principle which has been
During step 2, a plug of white petroleum jelly will be formed (See Fig. 1d) in
order to encase the active principle in petroleum jelly. This intricate step needs
to be carried out so as to avoid forming any air pockets which would compro
mise the formation of the white petroleum jelly "cocoon".
In order to complete the preparation of the container, it will be closed by two rigid plugs (See Fig. 1e).
In this closed form of the container, the active principle encapsulated with white
petroleum jelly can be transported to its processing station while being protected from light and oxidation phenomena.
- In order to carry out trie subsequent step 3, the small plug of the container will
be removed and the large plug of the container will be replaced by a perforated
plug (see Figs 3 and 4).
The container can thus be introduced into a vat (see Fig. 6), this vat and its ex-cipient contents being heated sufficiently so that the mixture of excipients which it contains is in a liquid form.
- During step 4, the solid petroleum jelly encapsulating the active principle will
melt upon contact with the mixture of heated excipients. It will therefore be able
to diffuse and be distributed homogeneously among the other excipients con-
tained in the vat of the mixer, while carrying the active principle solution with it.
This homogeneous distribution is facilitated by various factors, firstly by the
temperature of the excipients in the vat, lying between 70 and 90DC, which
therefore makes it possible to melt the petroleum jelly contained in the diffusion
container holding the active principle. The homogeneous distribution is also
subsequently facilitated by the grilles at the ends of the container, which only let
the active principle diffuse constantly and in a small quantity. The grilles there-
fore prevent the formation of large masses of semisolid petroleum jelly, which would carry a large fraction of the active principle with them, leading to poor distribution and therefore to poor homogenisation of tha final product.
The term "microdosed active principle" is intended to mean an active principle concentration of between 1 and 100 ppm, for example between 1 and 50 ppm, in particular between 1 and 10 ppm and preferably between 1 and 5 ppm.
In a preferred'embodiment, the active principle used in the method according to the invention is calcitriol; in particular, it will be preferable to use calcitriol at a final concentration of 3 ppm. The method according to the invention may of course be used to prepare com-
positions comprising a plurality of active agents, one or more of which are microdosed.
The term "weighing" is intended to mean a taring action, or more generally a way of determining the weight or mass of a product.
In order to avoid any risk of contamination with a toxic active principle, the weighing step will preferably be carried out in a fume cupboard with vertical laminar flow or in a sealed enclosure.
In order to avoid any inaccuracy, this weighing step will preferably be carried
out on a balance with an accuracy of 1/100 mg.
The term "container" is intended to mean any volume, receptacle or container
. ~ '
for introducing an active principle. In the present case, the invention relates more particularly to a container of the frustoconical type.
In order to facilitate the diffusion, it is preferable to use a conic frustum whose shape will provide it with the property of facilitating and increasing the flow rate of the fluid. The flow rate will furthermore be moderated in the wide part, and this will allow better dissolving of the active principle.
The term "metal container" is preferably intended to mean a stainless steel container.
In a particular embodiment of the present method, the container allowing optimal diffusion of the active principle calcitriol at a concentration of 3 ppm, in order to prepare the product Silkis, comprises the following parts (see Fig. 2):
1 - a metal conic frustum (see Fig. 3) made of stainless steel, which will
be covered with petroleum jelly during preparation of the formulation;
2 - two solid plugs and one large perforated plug (see Figs 2 and 4) made
of white polytetrafluoroethyiene. These plugs with a bayonet system will be
engaged on the container.
3 - the shape of the large perforated plug (see Fig. 4) and of the grille at
the small end of the container (see Fig. 3) will allow the molten petroleum
jelly to diffuse when it is in contact with the hot excipient, preventing the formation of lumps.
The fastening of the container onto a rod with a fixed height (depending on the configuration of the vat) is carried out by interlocking as schematised in Fig. 5. in this form with a fixed height, the container will be rotatable to allow homoge-
neous distribution of the active principle in the vat (see Fig. 6).
The term "rigid plug" is preferably intended to mean one made of polytetra-
fluoroethylene or stainless steel.
The term "petroleum jelly" is intended to mean any greasy substance derived from petroleum used in the composition of ointments. In this case, the invention relates more particularly to a petroleum jelly corresponding to the specifications in the European and American pharmacopoeias "Pharmeuropa" and "USP", with a viscosity of between 130,000 and 550,000 Cps. A petroleum jelly^which allows, maximal limitation of the paraffin oil exudation
phenomena is preferably used.
In order to allow the formation of a hermetic assembly, this operation will preferably be carried out in a vacuum, which makes it possible to form a hermetic assembly that can be kept for a longer time.
The term "mixture of excipients" is intended to mean any mixture containing two or more excipients, for example a mixture of petroleum jelly and paraffin, in particular a mixture of from 50 to 60% white petroleum jelly and from 40 to 50% paraffin.
The term "heated" is intended to mean any production of heat for raising the temperature of the excipients, so that the said excipients are in a liquid form.
Preferably, in, order to allow optimum homogenisation, it is important to comply
with a defined temperature and homogenisation time. In a preferred embodi
ment of the invention, for example, the temperature is between 70 and 90"C
and the homogenisation time is between two hours and seven hours, in particu
lar between three hours 30 minutes and five hours 30 minutes.
Details of the Figures:
FIG. 1A: measuring the weight of the container FIG. 1B::formihg and weighing the white petroleum jelly layer FIG. ICi depositing and weighing the calcitriol FjjSM D: forming the petroleum jelly plug FIG; 1E: closing the container with two rigid plugs " FIG 2: :plan view of the various parts of the container FIG. 3: conic frustum of the container (side view). FIG. 4: perforated plug with bayonet fitting (plan view) FIG. 5: interlocking of the container on the rod (plan view) FIG. 6: container fixed in the vat (plan view)
Example 1a: Weighing the calcitriol in the container.
It is first necessary to prepare a pot and a tube of petroleum jelly, which will be used for the step of weighing the calcitriol.
The pot and the tube are weighed in the following way:
- Putting the pot at its lid on the 8 kg balance;
- Reading the balance;
- Taking about 50 g of petroleum jelly and introducing it into the pot;
- Re-closing the pot and re-zeroing the balance;
- Putting the tube on the balance;
- Reading the balance;
- Taking about 50 g of petroleum jelly and introducing it into the tube; and
- closing the tube using the folding mechanism-
After this phase of preparing the petroleum jelly, the conic frustum is prepared
with the petroleum jelly in the following way;
It will first be necessary to put the stainless steel conic frustum, with its solid
lower plug and its solid upper plug fitted, on the plate of the balance, then the
balance is read before filling the container with petroleum jelly using a spatula.
The conic frustum should be 2/3 full and have a depression at its centre, where
the calcitriol will be introduced. The mass of petroleum jelly added will then be
noted before introducing the said conic frustum of petroleum jelly into the sealed
The vial of calcitriol is introduced into the sealed enclosure beforehand, so that
it is at the same'temperature as the compartment when it is weighed.
The calcitriol introduced into' the conic frustum will then be differentially
weighed, this step of introducing the calcitriol is carried out by progressive pouring, so as to avoid scattering the powder.
After this step of weighing the active principle, the depression containing the
calcitriol will be covered with the petroleum jelly contained in the tube.
The conic frustum formed in this way will be covered using the two solid plugs
with a bayonet fitting, and can be transported to its processing station while be-
ing protected from and risk of toxicity.
Example 1b: Manufacture of the product Silkis 3 ppm.
1a) the manufacturing vat is filled with white petroleum jelly so that it constitutes 56,2487% of the final formulation. This petroleum jelly is firstly pre-melted at a temperature allowing it to enter a liquid state, then heated in nitrogen and while stirring under temperature conditions for keeping this petroleum jelly in the liquid and homogeneous state.
1b) In parallel a kettle is filled with liquid paraffin so that it constitutes 43.75% of the final formulation. It is then heated in nitrogen and while stirring to a temperature equivalent to that used for the petroleum jelly in step 1a).
1c) The active principle will also be prepared in a sealed enclosure during this
first step. To that end, the caicitriol will be weighed in a petroleum jelly co-
coon, under inactinic light and in nitrogen, so that the caicitriol constitutes 0.0003% of the final formulation.
2) After these three compounds have been prepared, the hot paraffin is firstly introduced into the vat containing the petroleum jelly.
3) D.L alpha tocopherol is then added to this mixture of excipients, in a con
centration such that it constitutes 0.0001% of the final formulation.
4) This mixture of the three excipients will then be degassed and kept in ni
5) This is followed by a homogenisation phase, during which stirring is con
tinued in a vacuum.
6) After this homogenisation step, the conic frustum containing the caicitriol
will be introduced under inactinic light into the vat containing the three ex
7) Following this step, degassing and re-inerting with nitrogen will be carried
out in a vacuum.
8) This assembly will then subjected to the time necessary for homogenisa-
tion in a vacuum.
9) After this latter homogenisation phase, the formulation will be allowed to
cool in a vacuum in order to avoid any inclusion of air in the mixture.
10) The final step will be primary packaging in the liquid state in nitrogen, with
slow stirrina and under inactinic light.
1. A method for preparing a formulation containing microdosed calcitriol, the said method
being characterised by the following preparation steps:
1. weighing calcitriol in a container lined with petroleum jelly;
2. encapsulating the calcitriol with additional petroleum jelly;
3. introducing the container holding the calcitriol and the petroleum jelly into a mixer containing a mixture of excipients; and
4. diffusing the calcitriol in the mixer.
2. The method as claimed in claim 1, wherein in order to avoid any risk of contamination, the weighing step is carried out in a fume cupboard with vertical laminar flow or in a sealed enclosure.
3. The method as claimed in claim 1 or 2, wherein the volume for introducing the calcitriol is a container of the frustoconical type.
4. The method as claimed in any one of claims 1 to 3, wherein the said petroleum jelly has a viscosity of between 130,000 and 550,000 Cps.
5. The method as claimed in any one of claims 1 to 4, wherein the calcitriol has a final concentration of 3 ppm.
6. The method as claimed in claim 5, wherein the mixture of excipients consists of from 50 to 60% white petroleum jelly and from 40 to 50% paraffin.
|Indian Patent Application Number||5719/DELNP/2005|
|PG Journal Number||30/04/2010|
|Date of Filing||09-Dec-2005|
|Name of Patentee||GALDERMA S.A.|
|Applicant Address||ZUGERSTRASSE 8, 6330 CHAM, SWITZERLAND.|
|PCT International Classification Number||A61K 9/00|
|PCT International Application Number||PCT/FR2004/001116|
|PCT International Filing date||2004-05-07|