Title of Invention

NOVEL STRUCTURAL ANALOGS OF COROSOLIC ACID HAVING ANTI-DIABETIC AND ANTI-INFLAMMATORY PROPERTIES

Abstract

This invention relates to corosolic acid analogs of the general formula I, wherein R<sub>1</sub>}, R<sub>2</sub>, R<sub>3</sub>, R<sub>4</sub> and R<sub>5</sub> are as indicated below in each of said analogs: 3. R<sub>1</sub> = COC<sub>5</sub>H<sub>4</sub>N, R<sub>2</sub>= R<sub>3</sub>= H, R<sub>5</sub>= COOH 4. R<sub>1</sub> = COCH<sub>2</sub>NH<sub>2</sub>.HC<sub>1</sub>, R<sub>2</sub>= R<sub>3</sub>= H, R<sub>5</sub>= COOH ;:" 5. R<sub>1</sub> = COCH(CH<sub>3</sub>) NH<sub>2</sub>.HC<sub>1</sub>, R<sub>2</sub>= R<sub>3</sub>=R<sub>4</sub>= H, R<sub>5</sub>= COOH 8. R<sub>1</sub>=R<sub>2</sub>=R<sub>3</sub>=R<sub>4</sub>= H, R<sub>5</sub>= CONH<sub>2</sub> 9. R<sub>1</sub>=R<sub>2</sub>=R<sub>3</sub>=R<sub>4</sub>= H, R<sub>5</sub>= CONH<sub>5</sub> 10. R<sub>1</sub>=R<sub>2</sub>=R<sub>3</sub>=R<sub>4</sub>= H, R<sub>5</sub>= CONHCH<sub>2</sub>CH<sub>2</sub>NH<sub>2</sub> 11. R<sub>1</sub>=R<sub>2</sub>=R<sub>3</sub>=R<sub>4</sub>= H, R<sub>5</sub>= CO(CH<sub>2</sub>CH<sub>2</sub>hNH 12. R<sub>1</sub>=R<sub>2</sub>=R<sub>3</sub>=R<sub>4</sub>= H, R<sub>5</sub>= CONHCH<sub>2</sub>CH<sub>2</sub>OH 17. R<sub>1</sub>= R<sub>2</sub>= H, R<sub>3</sub> & R<sub>4</sub> = 0, R<sub>5</sub>= COOH 21. R<sub>1</sub>= R<sub>2</sub>=R<sub>3</sub>= H, R<sub>4</sub> = OH, R<sub>5</sub>= CONH<sub>2</sub> 22. R<sub>1</sub> = R<sub>2</sub>=R<sub>3</sub>= H, R<sub>4</sub> = OH, R<sub>5</sub>= COOCH<sub>3</sub> These compounds exhibit good hypoglycemic and 5-lipoxygenase inhibitory activities. They also inhibit tumour growth. Phannaceutical compositions containing known adjutants and the title compound are also within the scope of this invention.

Full Text

NOVEL STRUCTURAL ANALOGS OF COROSOLIC ACID HAVING ANTI-DIABETIC AND ANTI-INFLAMMATORY PROPERTIES TITLE OF THE INVENTION This invention relates to novel structural analogs of corosolic acid having anti-diabetic and anti-inflammatory properties. These compounds are found to exhibit potent hypoglycemic, 5-lipoxygenase inhibitory and antitumour activities. TECHNICAL FIELD Diabetes is perceived as a disorder of metabolism, where body’s natural ability to utilize food that has been broken down by digestion is vitiated. The body utilizes glucose, a major metabolic product from food, for energy and for cell growth. Glucose disperses throughout the body through the blood stream and enters cells with the help of a hormone called insulin. Insulin is produced by pancreas, a large gland beneath the stomach. In people with diabetes mellitus, either the pancreas does not produce enough insulin to move the glucose into the cells or the cells do not respond to the insulin, even though plenty is produced (Reaven, G. M., Role of insulin resistance in human disease; Diabetes, 1998, 37, 1595-1607). As a result of this impairment, glucose builds up in the blood stream and excreted out of the body without ever having been used as fuel. Untreated diabetes can lead to very serious chronic problems, including heart disease, kidney failure, blindness, nerve damage and amputations (Porte, D. et al., Science, 1996, 27, 699-700). Many experts believe that diabetes, cardiovascular disease and obesity all have a common factor linked by a condition called insulin resistance also known as Syndrome X (Bagchi, D., Syndrome X, The Diabetes, CVD and Obesity link, Health Products Business, June 2001, 62.). But with proper management, the risk of such problems can be greatly reduced. The management plan depends on the type of diabetes: insulin-dependent diabetes mellitus (IDDM) or noninsulin-dependent diabetes mellitus (NIDDM). BACKGROUND ART There are a number of agents currently available in the market for diabetes management, which belongs to various structural types. For example, thiazolidinediones, sulfonyl ureas, alpha-glucosidase inhibitors and biguanides are some of the drug types currently available in the market According to the American Diabetes Association, diabetes mellitus is estimated to effect 6% of the world population and the recent studies indicate that the number of diabetic patients could rise to 300 million by 2025. Worldwide sales of antidiabetic drugs reached 10 billion US dollars in 2002. Oral antidiabetics accounted for 63% of these sales and glucophase (metformin) was the leading product. With rising number of people suffering from diabetes worldwide, the market for diabetes medications could exceed $ 20 billion by 2006. In the natural products arena, a handful of herbal medications were proven to be effective against this terrible menace. For example, Fenugreek (Trigonella foenumgraeeum), Gymnema {Gymnema sylvestre) Jamun/Jambolan (Syzygiwn cumini\ Bitter melon/Karela (Momordica charantia) and Banaba (Lagerstroemia speciosd) are some of the products known to show fyypoglycemic activity. Natural antidiabetic treatments have gained popularity in the recent years because of their proven safety from long history of usage in traditional medicine and also present usage in herbal treatments. Banaba, Lagerstroemia speciosa L, has gotten the worldwide attention in the past few years as organic insulin. It is widely distributed in Philippines, as well as in Malaysia, South China and tropical Australia. Corosolic acid or colosolic acid (2a-hydroxyursolic acid, CAS No. 4547-24-4), a triterpenoid compound isolated from the banaba extract was found to be responsible for the antidiabetic activity. Banaba has long been recognized for the treatment of diabetes and also for maintenance of low blood pressure and improved kidney function in Philippines and other East Asian countries. Clinical studies confirmed the hypoglycemic effects of corosolic acid (Judy, W.V. et aL, /. EthnopharmacoL, 2003, 87(1), 115-7). Indian species of Lagerstroemia (Lagerstroemia parviflora, Lagerstroemia indica, Lagerstroemia spedosa, etc), which grows along east coast from Orissa to West Bengal, also produce corosolic acid. Matsuyama, US patent No. 6,485,760 (2002) described the blood sugar lowering effect of Lagerstroemia extract. Presently, there has been a tremendous surge in the demand for non-steroidal, plant based anti-inflammatory agents. 5-Lipoxygenase is the key enzyme for the biosynthesis of leukotrienes and 5(S)-HETE, the important mediators for inflammatory, allergic and obstructive process, from arachidonic acid. 5-Lipoxygenase is the target enzyme for identifying inhibitors, which have the potential to cope with a variety of inflammations and hypersensitivity-based human diseases including asthma, arthritis, bowl diseases such as ulcerative colitis and circulatory disorders such as shock and ischaemia. Scientists around the world have invested major effort during the last ten years, in identifying 5-lipoxygenase inhibitors from plant sources. Gum resin of Boswellia species known as Indian frankincense has been used as an anti-inflammatory agent in traditional Ayurvedic Medicine in India. The source of anti-inflammatory actions has been attributed to boswellic acids (Safayhi, H., et aL, Planta Medico, 1997, 63, 487-493 and J. Pharmacol Exp. Then, 1992, 261, 1143-46, both the journals published from USA), a group of triterpene acids isolated from the Boswellia resin (Padhy, R.S., et aL, Indian J. Chem., 1978, 16B, 176-178). During our search for new anti-inflammatory agents, we have observed, to the best of our knowledge for the first time that corosolic acid is a potential inhibitor of 5-LOX. The inhibitory activity was found to be on par with 3-O-acetyl-ll-keto-p-boswellic acid(AKBA). The olenane and ursane triterpenoids also gained prominence recently for their antiproliferative actions. As 5-lipoxygenase (5-LOX) is the first enzyme in the metabolic pathway leading to the formation of leukotrienes and eicosanoids that are important in carcinogenesis process, inhibitors of 5-LQX may thus have profound influence on the growth and apoptosis of various cancer lines (Yong S. Park, et. aL, Planta Medica, 2002, 68, 397-401). Boswellic acids, for example inhibited several leukemia cell lines in vitro and inhibited melanoma growth and induced apoptosis (Hostanska, K., et aL, Anticancer Res., 2002, 22(5), 2853-62). The acetyl boswellic acids were found to be unique class of dual inhibitors of human topoisomerages I and II a (Syrovets, T. et. aL, Mol Pharmacol, 2000, 58(1), 71-81). A number of oleanane and ursane tripenoids were found to be powerful inhibitors of nitric oxide production in macrophases, which can be correlated to their cancer chernoprevention activity (Honda, T. et aL, J. Med. Chenu, 2000, 43, 1866-77). Corosolic acid, which has the gross structure very similar to AKBA and other ursane derivatives, may thus hold promise as an antitumor agent. OBJECTS OF THE INVENTION The present invention was aimed at producing novel analogs of corosolic acid for structure-activity relationship studies. The main objective was to make analogs with enhanced water solubility and increased hypoglycemic activity. The functional groups that can be expended to make new analogs are carboxyl group, trisubstituted double bond and vicinal diol. The hydroxyl groups found utility to couple with moieties like natural acids and aminoacids, which not only improves water solubility but also presumed to enhance the biological recognition to the parent compound in the transport process. The acid function was utilized to attach highly polar amine moieties through an amide linkage. The same group can also be utilized to make ester compounds* by reacting with alcohols or halides. The lower alkyl esters can be reduced with LAH (lithium aluminum hydride) to introduce an additional alcohol group as depicted in structure 23. Oxidizing agents suitable for allylic oxidation, such as chromium trioxide can be utilized to generate 11-ketocorosolic acid compounds. Allylic oxidation using NBS (N-bromosucinimide) however yielded a lactone 26. The acylation transformation can be controlled to yield monoacylated compounds or diacylated compound by limiting the quantities of acylating agent. The general strategy to attach amino acid unit was coupling of BOC (tert-butoxycarbonyl) protected amino acids like glycine and alanine etc. to corosolic acid using DCC (1,3-dicyclohexylcarbodiimide), DMAP [4-(dimethylamino)-pyridine] followed’ - by deprotection of BOC group using HCl/dioxane to yield glycyl and alanyl derivatives, respectively, of corosolic acid. A still further aspect of the present invention is a method of treating diabetes, comprising administering to a human or animal subject in need thereof a treatment effective amount (e.g., an amount effective to treat, slow the progression of, etc.) of a compound as described above. The pharmaceutical compositions of the “compound” as used herein, includes the pharmaceutically acceptable salts of the compound Pharmaceutically acceptable salts are salts that retain the desired biological activity of the parent compound and do not impart undesired toxicological effects. Examples of such salts are (a) base addition salts formed from metal hydroxides, NH4OH, alkyl amines, pharmaceutically useful aniine compounds etc. (b) acid addition salts formed with inorganic acids, for example hydrochloric acid, hydrobromic acid, sulfiiric acid, phosphoric acid etc. on amine compounds represented by the formula I. Active compounds of the present invention may be produced by the procedures described herein or variations thereof, which will be apparent to those skilled in the art. The intermediates useful for producing the compounds of the formula I, described herein are also an aspect of the present invention, as are methods useful for producing such intermediates and active compounds. DESCRIPTION OF PREFERRED EMBODIMENTS The present invention is explained in greater detail in the following non-limiting examples. Example 1 2-O-Acetylcorosolic acid and 3-O-acetyIcorosolic acid (1): To an ice cold solution of corosolic acid (500 mg, 1.06 mmol) in pyridine (0.75 mL, 9.7 mmol) was added slowly acetic anhydride (0.1 mL) and continued the stirring for 2 h. The mixture was poured into crushed ice and vigorously stirred. The solid was filtered, washed with water, dried and subjected to silica gel column chromatography using hexane-ethyl acetate (10%) mixture as eluent to furnish a white solid (220 mg); IR (KBr): 3434, 2927, 2863, 1722, 1695,1456, 1256, 1030 cm-1; It is a mixture of 3-O-acetyl and 2-O-acetyl derivatives in the ratio 1:2.7. NMR data corresponds to major product (2-O-acetyl derivative); 1H NMR (400 MHz, CDC13) δ 0.78 (3H, s, CH3), 0.84 (3H, d, J= 6.0 Hz, CH3), 0.86 (6H, s, 2 x CH3), 0.95 (3H, d, J= 4.8 Hz, CH3), 1.06 (3H, s, CH3), 1.08 (3H, s, CH3), 2.06 (3H, s, -COCH3), 2.20 (1H, d, J=11.3 Hz, H-18), 3.20 (1H, d, J=10.0 Hz, H-3), 4.92-4.98 (1H, m, H-2), 5.24 (1H, br s, H-12); NMR data corresponds to minor product (3-O-acetyl derivative): 2.14 (s, -COCH3), 3.78-3.82 (m, H-2), 4.50 (d, J=10.0 Hz, H-3), 5.24 (br s, H-12); LCMS (negative ion mode): m/z 513 (M-H)3-O-Acetylcorosolic acid: IR (KBr): 3442, 2933, 2869, 1729, 1696, 1629, 1456, 1374, 1253,1038 cm’1; LCMS (negative ion mode): m/z 513 (M-H)”. Example 2 2,3-IM-O-acetylcorosolic acid (2): To a solution of corosolic acid (800 mg) in pyridine (5 mL) was added acetic anhydride (5 mL) and kept at rt for 16 h. The reaction mixture was worked up under the conditions noted in example 1, to give the diacetate, 2 (650 mg, 69%), m.p. 236-240 °C; IR (KBr): 3448, 2944, 2873, 1743, 1698, 1455, 1371, 1250, 1038, 962 cm-1; 1H NMR (400 MHz, CDC13)δ 0.77 (3H, s, CH3), 0.85 (3H, d, J= 6.3 Hz, CH3), 0.90 (6H, s, 2 x CH3), 0.95 (3H, d, J= 5.9 Hz, CH3), 1.07 (6H, s, 2 x CH3), 1.97 (3H, s, -COCH3), 2.05 (3H, s, -COCH3), 2.19 (1H, d, J=l 1.2 Hz, H-18), 4.75 (1H, d, J=10.3 Hz, H-3), 5.07-5.13 (1H, m, H-2), 5.24 (1H, br s, H-12); LCMS (negative ion mode): m/z 555 (M-H)Example 3 2-O-NicotinoylcorosoIic acid (3): To a mixture of corosolic acid (250 mg, 0.53 mmol), nicotinic acid (200 ing, 1.62 mmol) and DMAP (catalytic) in acetonitrile (50 mL) was added DCC (400 mg, 1.94 mmol) and stirred at rt for 24 h. The solids were filtered off and the solvent was evaporated The residue was chromatographed over silica gel column using chloroform-methanol (20%) as eluent to furnish 2-O-nicotinoylcorosolic acid (33 mg, 11%), which was crystallised from chloroform-hexane, m.p. 212-216 °C; IR (KBr): 3434, 2928, 2871, 1722, 1594, 1456, 1288, 1132, 955 cm-1; lH NMR (400 MHz, CDC13) δ 0.81 (3H, s, CH3), 0.84 (3H, d, J= 6.3 Hz, CH3), 0.92 (3H, s, CH3), 0.95 (3H, d, J=6.3 Hz, CH3), 1.11 (6H, s, 2 x CH3), 1.12 (3H, s, CH3), 3.39 (1H, d, J= 9.9 Hz, H-3), 5.24-5.27 (1H, br s, H-12), 7.37-7.41 (1H, m, Ar-H), 8.29 (1H, d, J=7.7 Hz, Ar-H), 8.78 (1H, d, J=3.5 Hz, Ar-H), 9.22 (1H, s, Ar-H); LCMS (negative ion mode): m/z 576 (M-H)-. Example 4 2-O-GlycylcorosoIic acid hydrochloride (4): A mixture of corosolic acid (200 mg, 0.42 mmol), BOC protected glycine (82 mg, 0.47 mmol) and DMAP (30 mg) in dry dioxane (2 mL) at 0 °C was treated with DCC (130 mg, 0.63 mmol) under vigorous stirring. After 3 h, the reaction mixture was worked up as described in example 3, to give 2-O-(N-B0C-glycyl)corosolic acid (200 mg). A solution of 2-(N-BOC-glycyl)corosolic acid (200 mg) in CH2C12 (2 mL) was cooled to 0 °C and treated slowly with 2 mL of 1 N HC1 in dioxane. After 30 min, the stirring was continued at rt for another 2 h. The reaction mixture was diluted with hexane (5 mL) and the precipitated solid was filtered, washed with hexane and dried to afford a white powder of 2-O-glycylcorosolic acid hydrochloride (190 mg), m.p. 268-272 °C; IR (KBr): 3432, 2979, 2926, 2859, 1749, 16905 1461, 1243, 1050 cm-1; 1H NMR (400 MHz, CD3OD) δ 0.82-1.14 (7 x CH3), 2.2 (1H, brd, J= 10.0 Hz), 3.80 (1H, brm, H-3), 3.88 (2H, s, OCOCH2NH3CI), 4.66 (1H, d, J= 9.9Hz, H-2), 5.24 (1H, m, H-12); LCMS (positive ion mode); m/z 530 (M-C1)+. Example 5 2-O-AlanyIcorosoIic acid hydrochloride (5): A mixture of corosolic acid (500 mg, 1.06 mmol), BOC protected alanine (240 mg, 1.23 mmol) and DMAP (75 mg) in dry dioxane (2 mL) at 0 °C was treated with DCC (327 mg, 1.59 mmol) under the conditions noted in example 4, obtained 2-O-(N-BOC-alanyl)corosolic acid (320 mg). This was deprotected as in example 4, to give 2-O-alanylcorosolic acid hydrochloride (250 mg) as white powder, m.p. 234-238 °C; IR (KBr): 3433, 2928, 2859, 1740, 1692, 1621, 1459, 13695 1245, 1107, 1041 cm-3; 1H NMR (400 MHz, DMSO-d6) δ 0.57-1.35 (24H, m, 8 x CH3), 4.68-4.76 (1H, br s, -CH-N), 4.88-5.05 (1H, br s, H-3), 5.08-5.20 (1H, br s, H-12), 8.10-8.60 (3H, br s, NHa+); LCMS (positive ion mode): m/z 544 (M-Cl)Example 6 Methyl 2-O-(3,4,5-trimethoxycinnamoyI)corosolate (6): To a mixture of methyl corosolate (100 mg, 0.21 mmol), 3,4,5-trimethoxycinnamic acid (73 mg, 0.31 mmol) and DMAP (12 mg, 0.1 mmol) in CH2Ch (1-5 mL) cooled in an ice-water bath was added slowly DCC (85 mg, 0.41 mmol) in 0.5 mL of CH2C12. The mixture was allowed to reach ambient temperature and continued the stirring. After 2 h, the mixture was worked-up under the conditions noted in example 4, to furnish methyl 2-0-(3,4,5-trimethoxycinnamoyl)corosolate (90 mg, 62%), m.p. 198-206 °C; IR (neat): 3460, 2927, 2854, 1717, 1632, 1583, 1457, 1263, 1098, 1024, 805 cm-1; LCMS (positive ion mode): m/z 729 (M+Na)+. Example 7 2a,3p-Dihydroxyurs-12-en-28-oic acid 2,3-cydicsulphate (7): To a mixture of corosolic acid (200 mg, 0.42 mmol)) and pyridine (0.34 mL, 4.2 mmol) in THF (1.5 mL) was slowly added thionyl chloride (40 µL, 4.2 mmol) and stirred at rt for 2 h. The reaction mixture was poured into 0.2N HCI (20 mL). The white precipitate was filtered, washed with water and dried under vacuum. This sulphite (100 mg) was dissolved in acetonitrile (1 mL), water (0.8 mL) and CH2CI2 (1 mL) and treated with a solution of ruthenium trichloride monohydrate (100 µg) in acetonitrile (1 mL) followed by NaIO4 (300 mg). The stirring was continued for 36 h. The mixture was poured into water and extracted with ethyl acetate. The organic layer was washed with brine, dried over Na2SO4 and evaporated. The residue (90 mg) was subjected to silica gel column chromatography using hexane-ethyl acetate (20%) as eluent to furnish cyclicsulphate derivative 7 (40 mg), m.p. 182-186 °C; IR (neat): 3431, 2926, 2871, 1693, 1459, 1386, 1211, 995, 959 cm-1. LCMS (negative ion mode): m/z 533 (M-H). Example 8 Corosolamide (8): A mixture of diacetylcorosolic acid (300 mg) and thionyl chloride (2 mL) was refluxed for 1 h and the excess reagent was removed under reduced pressure to give acid chloride. This crude acid chloride in THF (1 mL) was added drop wise to a stirred solution of cone, ammonia (5 mL) at ice-cold temperature for 5 min and continued stirring at the same temperature for 2 h. The reaction mixture was poured into ice-cold water and extracted with ethyl acetate. The organic layer was washed with dil. H2SO4, water, brine and dried over sodium sulfate. The solution was filtered and the solvent evaporated to give diacetyl corosolamide (300 mg). A solution of diacetyl corosolamide (300 mg) and methanolic-potassium hydroxide (4%, 25 mL) was refluxed for Ih. The solvent was evaporated undo: reduced pressure and diluted with ice-cold water and acidified with dil. H2SO4. The solution was extracted with ethyl acetate and the organic layer was washed with water, brine and dried over sodium sulfate. The residue obtained after evaporation of the solvent was chromatographed over silica gel column using chloroform-methanol (10%) as eluent to furnish corosolamide (200 mg, 67%), which was recrystallised from chloroform-hexane, m.p. 208-210 °C; IR(KBr): 3495, 2927, 2870, 1671, 1602, 1457, 1376, 1049, 959 cm-1; 1H NMR (400 MHz, CDC13)δ 0.83 (3H, s, CH3), 0.86 (3H, s, CH3), 0.87 (3H, d, J= 6.7 Hz, CH3), 0.96 (3H, br s, CH3), LOO (3H, s3 CH3), 1.04 (3H3 s, CH3), 1.11 (3H, s, CH3)5 3.00 (1H, d, J= 9.4 Hz, H-3), 3.67-3.73 (1H, m, H-2), 5.32 (1H, br s, H-12), 5.85 (2H, br s, CONH2); LCMS (negative ion mode): m/z 470 (M-H)Example 9 N-Phenylcorosolamide (9): Reaction of diacetylcorosolyl chloride (100 mg) with aniline (1 mL) in THF (10 mL) and triethyl amine (1 mL) under the conditions noted in example 8 gave N-phenylcorosolamide, which was crystallised from chloroform-methanol (60 mg, 61%), m.p. 168-174 °C; IR (KBr): 3408, 2927, 2868, 1652, 1599, 1529, 1502, 1442, 1312, 1235, 1048 cm-1; lH NMR (400 MHz, CDC13) δ 0.70 (3H, s, CH3), 0.80 (3H, s, CH3), 0.93-1.02 (12H, m, 2 methyl singlets merge with 2 methyl doublets), 1.14 (3H, s, CH3), 3.00 (1H, d, J= 7.76 Hz, H-3), 3.6-3.7 (1H, br s, H-2), 5.45-5.50 (1H, br s, H-12), 7.07 (1H, br s, Ar-H), 7.28 (1H, br s, Ar-H), 7.44 (2H, br s, Ar-H), 7.67 (1H, br s, Ar-H); LCMS (positive ion mode): m/z 548 (M+H)+. Example 10 N-(2-Aminoethyl)corosolamide (10): Reaction of diacetylcorosolyl chloride (200 mg) with ethylene diamine (1.0 g) in THF (10 mL) and work-up under the conditions noted in example 8 furnished N-(2-aminoethyl)corosoiamide (110 mg, 60%), m.p. 118-120 °C; IR (KBr): 3403, 2926, 1633, 1527, 1454, 1383, 1048 cm-1; 1H NMR (400 MHz, DMSO-d6) δ 0.69 (3H, s, CH3), 0.72 (3H, s, CH3), 0.84 (3H, d, J= 6.0 Hz, CH3), 0.92-0.94 (9H, 2 br s, 2 methyl singlets and a methyl doublet), 0.99 (2H, d, J=7.2 Hz, -NC0-CH2-), 1.05 (3H, s, CH3), 2.17 (1H, d, J=11.2 Hz, H-18), 2.57 (2H, q, J=7.0 Hz, NH2-CH2), 2.70 (2H, t, J=6.7 Hz, NH2-CH2), 2.75 (1H, d, J= 9.2 Hz, H-3), 4.3-4.4 (1H, m, H-2), 5.23 (1H, br s, H-12); LCMS (positive ion mode): m/z 515 (M+H)+. Example 11 N-(CorosolyI)piperazine (11): Reaction of diacetylcorosolyl chloride (100 mg) with piperazine (200 mg) in THF (10 mL) and triethyl amine (2 mL) and work-up under the conditions noted in example 8 gave N-(corosolyl)piperazine, which was crystallised from chloroform-hexane (50 mg, 52%), m.p. 226-230 °C; IR (KBr): 3434, 2924, 2868, 1628, 1455, 1226, 1049 ran1; 1H NMR (400 MHz, CDC13) δ 0.76 (3H, s, CH3), 0.83 (3H, s, CH3), 0.87 (3H, d, J= 6.3 Hz, CH3), 0.94 (3H, d, J=6.2 Hz, CH3), 0.99 (3H, s, CH3), 1.03 (3H, s, CH3), 1.08 (3H, s, CH3), 2.44 (1H, d, J=8.6 Hz, H-1.8), 2.83 (4H, s, N-CH2), 3.00 (1H, d, J= 9.5 Hz, H-3), 3.58 (4H, d, J=3.4 Hz, N-CH2), 3.66-3.72 (1H, m, H-2), 5.23 (1H, br s, H-12); LCMS (positive ion mode): nVz 541 Example 12 N-(2-HydroxyethyF)corosoIainide (12): Reaction of diacetylcorosolyl chloride (100 mg) with 2-aminoethanol (1 mL) in THF (10 mL) and triethyl amine (1 mL) under the conditions noted in example 8 gave N-(2-hydroxyethyl)corosdlamide, which was crystallised from chloroform-hexane to obtain 12 (43 mg, 47%), m.p. 152-158 °C; IR (KBr): 3408, 2963, 2926, 2856, 1632, 1529, 1455, 1262, 1094, 1026, 802 cm-1; 1H NMR (400 MHz, CDC13)δ 0.81 (3H, s, CH3), 0.83 (3H, s, CH3), 0.87 (3H, d, J= 6.2 Hz, CH3), 0.96 (3H, br s, CH3), 1.00 (3H, s, CH3), 1.04 (3H, s, CH3), 1.11 (3H, s, CH3), 2.98 (1H, br s), 3.00 (1H, d, J= 9.3 Hz, H-3), 3.21-3.26 (1H, m), 3.44-3.49 (1H, m, H-2), 3.68 (3H, br s, N-CH2CH2-), 5.34 (1H, s, H-12), 6.34 (1H, br s); LCMS (negative ion mode): m/z 514 (M-H)”. Example 13 Methyl corosolate (13): A mixture of corosolic acid (2.0 g, 4.34 mmol), iodomethane (1 mL, 16 mmol), potassium carbonate (4.5 g, 32.6 mmol) and acetone (60 mL) was stirred at rt for 16 h. After completion of the reaction, the solids were filtered off and the solvent was evaporated undo: reduced pressure. The residue was chromatographed over silica gel column using chloroform-methanol (10%) as eluent to furnish methyl corosolate (1.7 g, 83%), which was recrystallised from chloroform-hexaiie, m.p. 208-210 °C; IR (KBr): 3432, 2946, 2872, 1728, 1455, 1230, 1197, 1049 cm-1;.1H NMR (400 MHz, CDC13) δ 0.75 (3H, s, CH3), 0.83 (3H, s, CH3), 0.85 (3H, d, J- 6.5 Hz, CH3), 0.94 (3H, d, J= 5.7 Hz, CH3), 0.99 (3H, s, CH3), 1.03 (3H, s, CH3), 1.08 (3H, s, CH3), 2.23 (1H, d, J=11.0 Hz, H-18), 3.0 (1H, d, J= 8.4 Hz, H-3), 3.60 (3H, s, -COOCH3), 3.62-3.71 (1H, m, H-2), 5.25 (1H, t, J= 3.4 Hz, H-12); LCMS (negative ion mode): m/z 485 (M-H)’. Example 14 Methyl diacetylcorosolate (14): Reaction of diacetylcorosolic acid (500 mg, 0.9 mmol) with iodomethane (0.25 mL, 4.0 mmol), potassium carbonate (1.0 g, 7.2 mmol) and acetone (25 mL) under the conditions noted in example 13 gave methyl diacetylcorosolate (0.4 g, 78%), which was crystallised from aq. methanol to obtain 14, m.p. 138-140 °C; IR (KBr): 2943, 1742, 1243, 1036, 964 cm-1; 1H NMR (400 MHz, CDCI3) δ 0.75 (3H, s), 0.85 (3H, d, J=6.4 Hz), 0.90 (3H, s), 0.91 (3H, s), 0.94 (3H, d, J=5.9 Hz), 1.07 (6H, s), 1.97 (3H, s), 2.05 (3H, s), 2.23 (1H, d, J-11.4 Hz, H-18), 3.60 (3H, s), 4.75 (1H, d, J=10.3 Hz, H-3), 5.07-5.14 (1H, m, H-2), 5.23-5.24 (1H, m. H-12); LCMS (positive mode): 594 (M+l)+. Example 15 Methyl 11-ketocorosolate (15): Methyl corosolate (400 mg) was acetylated using pyridine (0.5 mL) and acetic anhydride (0.5 mL) under the conditions noted in example 2 to furnish methyl diacetylcorosolate (450 mg), which was dissolved in 1,4-dioxane (16 mL) and treated with N-bromosuccinimide (472 mg), water (1.6 mL) and ‘ calcium carbonate (472 mg). The reaction mixture was subjected to vigorous stirring for 3 h, and then filtered. The mother liquor was poured into cold water and extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate and evaporated. The residue (360 mg) in methanol (2 mL) was added 8N KOH solution (1 mL) and stirred at 65 °C for 1 h, then poured into ice cold water, acidified with 2N HC1 and extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate and evaporated. The residue (340 mg) was purified over silica gel column using hexane-ethyl acetate (25%) as eluent to furnish methyl 11-ketocorosolate (240 mg), m.p. 101-105 °C; IR (neat): 3416, 2926, 2858, 1728, 1659, 1457, 1388, 1201, 1048 cm-1; 1H NMR (400 MHz, CDC13) δ 0.84 (3H, s, CH3), 0.87 (3R d, J=6.5 Hz, CHO, 0.91 (3H, s, CH3), 0.97 (3H, d, J=6.3 Hz, CH3), 1.05 (3H, s, CH3), 1.19 (3H, s, CH3), 1.30 (3H, s, CH3), 2.35 (1H, s), 2.42 (1H, d, J=10.9 Hz), 3.02 (1H, d, J-9.5 Hz), 3.16 (1H, dd, J=12.6 & 4.3 Hz), 3.61 (3H, s, CH3), 3.77 (1H, m, H-2), 5.61 (1H, s, H-12); LCMS (positive ion mode): m/z 501 Example 16 Methyl diacetyl-ll-ketocorosolate (16): Reaction of methyl diacetylcorosolate, (500 mg, 0.9 mmol) in 1,4-dioxane (20 mL) with N-bromosuccinimide (0.75 g, 4.2 mmol) and calcium carbonate (0.75 g, 7.5 mmol) in water (2 mL) under the conditions noted in example 15 gave methyl diacetyl-ll-ketocorosolate (300 mg, 59%), which was crystallized from aq. methanol to obtain 16, m.p. 264-266 °C; IR (KBr): 2952, 1734, 1660, 1241, 1038, 985 cm-1; 1H NMR (400 MHz, CDC13) δ 0.86 (3H, d, J-6.4 Hz), 0.89 (3H, s), 0.91 (3H, s), 0.93 (3H, s), 0.97 (3H, d, J=6.3 Hz), 1.25 (3H, s), 1.29 (3H5 s), 1.95 (3H, s), 2.04 (3H, s), 3.18 (1H, dd, J=12.8, 4.6 Hz, H-18), 3.60 (3H, s), 4.72 (1H, d, J-10.3 Hz, H-3), 5.20-5.26 (1H, m3 H-2), 5.61 (1H, s, H-12); LCMS (positive ion mode): m/z 608 (M+H)+. Example 17 11-Ketocorosolic acid (17): A mixture of corosolic acid (400 mg, 0.85 mmol), pyridine (0.4 mL, 5.1 mmol) and acetic anhydride (1.5 mL, 15.2 mmol) was stirred at rt for 6 h. To the cooled reaction mixture after diluting with acetic acid (1.5 mL) and acetic anhydride (2 mL) was added chromium trioxide (254 mg) and stirred for 5 h. The reaction mixture was poured into ice-cold water and the precipitated solid was filtered and washed with water. The solid in methanol (4 mL) was treated with 8N KOH (2 mL) and stirred at rt for 14 h and then the mixture was filtered through celite. The mother liquor was poured into ice water, acidified and extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulphate and evaporated. The residue (390 mg) was purified over silica gel column using hexane-ethyl acetate (30%) as eluent to furnish 11-ketocorosolic acid (60 mg), m.p. 238-242 °C; IR (neat): 3417, 2927, 2857, 1692, 1659, 1460, 1386, 1051, 974 cm-1; LGMS (negative ion mode): m/z 485 (M-H)Example 18 Diacetyl-ll-ketocorosolic acid (18): Reaction of diacetyl corosolic acid (500 mg, 0.9 mmol) in dichloroethane (2 mL), acetic acid (2 mL) and water (1 mL) with a solution of chromium trioxide (1.5 g, 15 mmol ), acetic acid (2 mL) and water (2 mL) under the conditions noted in example 17 gave diacetyl-ll-ketocorosolic acid (200 mg, 39%), which was crystallized from chloroform-hexane to obtain 18, m.p. 318-320 °C; IR (KBr): 3184, 2975, 1742, 1641, 1253, 1036 cm-1; 1H NMR (400 MHz, CDC13) δ 0.86 (3H, d, J=6.4 Hz), 0.90 (3H, s), 0.91 (6H, s), 0.98 (3H, d, J=6.2 Hz), 1.26 (3H, s), 1.30 (3H, s), 1.95 (3H, s, -OCOCH3), 2.05 (3H, s, -OCOCH3), 3.18 (1H, dd, J=12.7, 3.2 Hz, H-18), 4.72 (1H, d, J=10.3 Hz, H-3), 5.19-5.26 (1H, m, H-2), 5.61 (lH/s,H-12); LCMS (negative mode): 569 (M-H)Example 19 2a,3β-Dihydroxyurs-12-en-ll-one-28-oic acid 23-cyclicsulphate (19): To a mixture of corosolic acid-2,3-sulfite (1.1 g, 2.12 mmol), dichloromethane (7 mL) and acetonitrile (4 mL) was added ruthenium chloride (2 mg) in acetonitrile (2 mL), followed by sodium periodate (L5 g). After stirring the mixture at rt for 2 h, an additional amount (0.5 g) of sodium periodate was added and after 2 h of stirring, the reaction mixture was worked up under the conditions noted in example 7 to give 2,3-cyclicsulphate derivative 19 (600 mg), m.p. 210-216 °C; IR (neat): 3429, 2924, 2356, 1705, 1658, 1618, 1380, 1206 cm-1; 1H NMR (400 MHz, DMSO-d6) δ 0.82 (3H, d, J=6.3 Hz, CH3), 0.90 (3H, s, CH3), 0.94 (3H, s, CH3), 0.95 (3H, d, J=6.4 Hz, CH3), 1.07 (3H, s, CH3), 1.20 (3H, s, CH3), 1.31 (3H, s, CH3), 3.20 (1H, dd, J=11.6 & 4.2 Hz? H-18).. 4.63 (1H, d, J= 10.4 Hz, H-3), 5.20-5.30 (1H, m, H-2), 5.44 (1H, s, H-12); LCMS (negative ion mode): m/z 547 (M-H)”. Example 20 11-Ketocorosolamide (20): Diacetyl-11-ketocorosolyl chloride (prepared from the 11-ketoacid, 150 mg and thionyl chloride 2 mL) was dissolved in THF (2 mL) and the solution was added dropwise to a stirred solution of cone, ammonia (5 mL) at ice oold temperature for 5 min and the solution was stirred at the same temperature for 2 h. The reaction mixture was worked up as described in example 8 to furnish 11-ketocorosolamide (40 mg, 31%), m.p. 220-222 °C; IR (KBr): 3427, 2970, 2930, 2871, 1659, 1459, 1384, 1200, 1048, 971 cm1; 1H NMR (400 MHz, DMSO-d6) δ 0.72 (3H, s, CH3), 0.82 (3H, d, J= 6.3 Hz, CH3), 0.89 (3H, s, CH3), 0.93 (3H, s, CH3), 0.95 (3H, d, J=6.3 Hz, CH3), 1.08 (3H, s, CH3), 1.27 (3H, s, CH3), 2.32 (1H, s), 2.36 (1H, d, J=ll.l Hz, H-18), 2.75 (1H, dd, J=12.7 & 4.2 Hz), 2.86 (1H, dd, J=12.7 & 4.2 Hz), 3.48 (1H, br s, H-2), 4.23 (1H, d, J=3.8 Hz), 4.34 (1H, d, J=3.8 Hz), 5.47 (1H, s, H-12), 6.83 (1H, s, OH), 6.97 (1H, s, OH); LCMS (positive ion mode): m/z 486 (M+H)+. Example 21 11-Hydroxycorosolamide (21): To a magnetically stirred ice cold (10-15 °C) solution of 11-ketocorosolamide (50 mg, 0.10 mmol) in ethanol (10 mL) was added sodium borohydride (200 mg, 5.26 mmol) and the solution was slowly brought to rt and stirred for 14 h. After completion of the reaction, the mixture was poured into ice-cold water and acidified with dil HCl. The solution was extracted with ethyl acetate and the organic layer was washed with water, brine and dried over sodium sulfate. The residue obtained after evaporation of the solvent was chromatographed over silica gel column using chloroform-methanol (95:5) as eluent to give 11-hydroxycorosolamide (20 mg, 40%), which was crystallised from chloroform-methanol, m.p. 196-198 °C; IR (KBr): 3432, 2929, 1659, 1600, 1383, 1048, 968 cm-1; 1H NMR (400 MHz, DMSO-d6) δ 0.73 (3H, s, CH3), 0.77 (3H, s, CH3), 0.90-0.94 (9H, m, 2 methyl doublets and a methyl singlet), 1.02 (3H, s, CH3), 1.11 (3H, s, CH3), 2.11 (1H, d, J=10.7 Hz, H-18), 2.42-2.46 (1H, m), 2.73 (1H, dd, J=9.1 & 3.7 Hz, H-3), 3.42 (1H, m, H-2), 4.01- 4.03 (2H, m, H-ll & 11-OH), 4.15 (1H, br s, NH2), 4.28 (1H, br s, NH2), 5.17 (1H, s, H-12), 6.68 (1H, s, OH), 6.73 (1H, s, OH); LCMS (negative ion mode): m/z 486 (M-H)Example 22 Methyl ll-hydroxycorosolate (22): To a magnetically stirred ice cold (10-15 °C) solution of methyl 11-ketocorosolate (360 mg, 0.72 mmol) in ethanol (40 mL) was added sodium borohydride (1.0 g, 26 mmol) and the solution was slowly brought to rt and stirred for 14 h. After completion of the reaction, the mixture was worked up as described in example 21 to give methyl ll-hydroxycorosolate (32 mg), m.p. 148-152 °C; IR (Neat): 3416, 2927, 2872, 1719, 1648, 1455, 1388,1220, 1146, 1047, 9995 960, 770 cm-1; ]HNMR(400MHz, CDC13)δ 0.86(6H, s, 2xCH3), 0.95 (3H, d, J= 6.0Hz, CH3)5 1.01-1.03 (12H, 3 methyl singlets and a methyl doublet), 2.31 (1H, d, J=11.3 Hz, H-18), 2.44 (1H, dd, J=12.0 & 4.2 Hz), 3.02 (1H, d, J-9.3 Hz, H-3), 3.80-3.83 (1H, m, H-2), 4.39 (1H, m, H-ll), 5.35 (1H, d, J=3.8 Hz, H-12); LCMS (negative ion mode): m/z 501 (M-H)-. Example 23 Corosolinol (23): To an ice cold dispersion of lithium aluminum hydride (97 mg) in THF (3 mL) was slowly added methyl corosolate (500 mg) in THF (1 mL) and stirred for 2 h. The reaction mixture was diluted with ethyl acetate (3 mL) and poured into ice water. The mixture was acidified with 2 N HC1 and extracted with ethyl acetate (50 mL). The organic layer was washed with brine, dried over Na2SO4 and evaporated. The residue (400 mg) was chromatographed over silica gel column using hexane-ethyl acetate (80:20) as eluents to yield corosolinol (220 mg), m.p. 140-146 °C; IR (neat): 3392, 2926, 2867, 1619, 1456, 1388, 1047, 1024, 760 cm”1; lH NMR (400 MHz, CDC13) δ 0 81 (3H, 6, J=5 4Hz, CH3), 0.84 (3H; s= CH3); 0.94 (3H? & J=5.3 Hz, CH3), 0.99 (3H, s, CH3), 1.03 (3H, s, CH3), 1.04 (3H, s, CH3), 1.11 (3H, s, CH3), 2.03 (1H, dd, J=12.3 & 4.5 Hz), 3.01 (1H, d, J=9.6 Hz), 3.19 (1H? d, J=11.0 Hz), 3.53 (1H, d, J=11.0 Hz), 3.70 (1H, m, H-2), 5.15 (1H, t, J=3.4 Hz, H-12); LCMS (negative ion mode): m/z 457 (M-H)”. Example 24 Corosolinal (24): To a cooled solution of Dess-Martin Periodinane (23 mg) in CH2CI2 (2 mL) was slowly added corosolinol (20 mg) dissolved in CH2CI2 (2 mL) and the solution was allowed to ambient temperature and continued the stirring for 2 h. The mixture was diluted with Et2O (20 mL) and poured into an ice-cold mixture of Na2S2O3.5H2O (90 mg) in saturated aqueous NaHCO3 (5 mL). The layers were separated and the organic layer was washed with saturated aqueous NaHCO3 (10 mL), water (20 mL), brine (20 mL) and dried over MgSO4. The solution was filtered and evaporated to give corosolinal (15 mg) as a colorless oil. IR (neat): 3433, 2925, 2855, 1721, 1451, 1387,1094 cm”1; LCMS (negative ion mode): m/z 455 (M-H)’. Example 25 Corosolyl tri-O-methylgallate (25): A mixture of tri-O-methylgallic acid (200 mg, 0.9 mmol) and SOC12 (0.5 mL ) was refluxed for 0.5 h. The excess reagent was removed under high vacuum and the residue in CH2CI2 (1 mL) was added to a mixture of corosolic acid (200 mg, 0.42 mmol) and DMAP (30 mg) in dioxane (5 mL). The reaction mixture was stirred at rt for 2 h and then poured into ice-cold water. The mixture was extracted with ethyl acetate (60 mL) and the organic layer was washed with 0.1 N HC1 (40 mL), water (40 mL), brine and dried over Na2SO4. The residue obtained after evaporation of the solvent was chromatographed over silica gel column using hexane-ethyl acetate (85:15) as eluent to yield corosolyl tri-O-methylgallate (25) as a white solid (75 mg), m.p. 158-162 °C; IR (KBr): 3439, 2930, 2853, 1793, 1728, 1627, 1584, 1460, 1336, 1233, 1129, 1019 cm’1; 1H NMR (400 MHz, CDC13) δ 0.83 (3H, s, CH3), 0.89 (3H, s, CH3), 0.99 (3H, s, CH3), 1.04 (3H, s, CH3), 1.13 (3H, s, CH3), 1.25 (3H, s, CH3), 2.35 (1H, s), 2.30 (1H, d, JN11.0 Hz), 3.00 (1H, d, J=9.3 Hz), 3.70 (1H, brm), 3.90 (9H, s, 3 x CH3), 5.37 (1H, s, H-12), 7.28 (2H, s, Ar-H); LCMS (positive ion mode): m/z 689 (M+ Na)+. Example 26 2a,3p-Dihydroxyurs-12-en-ll,28-olide (26): To a solution of corosolic acid (200 mg) in dioxane (6 mL) and water (0.6 mL) was added NBS (188 mg) and CaCO3 and the mixture stirred at rt for 4 h. The mixture was poured in to ice water (30 mL) and extracted with ethyl acetate (2 x 30 mL). The combined organic layer was washed with water (30 mL) followed by brine and dried over Na2SO4. The solvent was evaporated and the residue was subjected to silica gel column chromatography using hexane and ethyl acetate mixtures as eluents to obtain the lactone, 26 as a semisolid (60 mg); IR (neat): 3339, 2925, 2854, 1763, 1465 1261, 1021 cm-1; 1H NMR (400 MHz, CDC13) δ 0.75-1.25 (7 x CH3), 3.02 (1H, br s), 3.75 (1H, brm), 5.56 (1H, brd, J=8.2 Hz), 5,98 (1H, brd, J-8.2 Hz); LCMS (negative ion mode): m/z 469 (M-H)~. Hypoglycemic activity: Hypoglycemic activity was tested by the inhibition of sucrose-induced raise in serum glucose levels (SGL), by the test substances in Albino wistar rats. The procedure involves fasting the rats for overnight at ad libitum water, numbered weighed and randomly divided into groups of six animals each. Prior to treatment blood samples were drawn from sinus orbital plexus of all animals using heparin coated glass capillaries under mild ether anesthesia. The blood samples were tested for serum glucose levels using enzymatic GOD/POD method. Optical densities were measured at 500 nm, SGL was calculated as follows. SGL = (test OD / Standard OD) x 100 and the results were expressed in mg/dL. All the groups were treated orally with corresponding test substances, standard, vehicle (5% gum acacia). After 30 minutes, all animals were given 20 mL/kg of 20% sucrose solution orally using gastric tube. One hour after treatment, blood samples were drawn again under rnild ether anesthesia and tested for serum glucose levels in a same procedure as described above for initial serum glucose estimation. The data was subjected to statistical treatment using t-test and inhibitory rate was calculated by comparing mean increase in serum glucose levels of control and treated groups. 5-Lipoxygenase activity: The corosolic acid analogs were screened for their 5-Lipoxygenase inhibitory potential using colorimetric method. The assay mixture contained 50 mM phosphate buffer pH 6.3, 5-Lipoxygenase, various concentrations of test substances in dimethyl sulphoxide and linoleic acid in a total volume of 0.5 mL, after 5 min incubation of above reaction mixture, 0.5 mL ferric xylenol orange reagent was added and OD was measured after two minutes at 585 nm using spectrophotometer. Controls were run along with test in a similar manner except using vehicle instead of test substance solution. Percent inhibition was calculated by comparing absorbance of test solution with that of control. Brine shrimp lethality: Brine shrimp (Anemia salina) nauplii were hatched using brine shrimp eggs in a conical shaped vessel (1 L), filled with sterile artificial sea water (prepared using sea salt 38 g/L and adjusted to pH 8.5 using 1 N NaOH) under constant aeration for 48 h. After hatching, 10 nauplii were drawn through a pepette and placed in each vial containing 4.5 mL brine solution and added various concentrations of drug solutions and volume was made upto 5 mL using brine solution and maintained at 37°C for 24 h under the light of incandescent lamps and surviving larvae were counted. Each experiment was conducted along with control (vehicle treated), at various concentrations of the test substance in each set that contains 6 tubes and the average results are reported. The percentage lethality was determined by comparing the mean mortal larvae of test and control tubes. LC50 values were obtained from the plot of concentration (µg) vs. percentage lethality. Podophyllotoxin was used as a positive control. The corosolic acid analogs of this invention are found to show better hypoglycemic activity (Table 1; hypoglycemic activity is expressed in serum glucose level inhibitory rate values; higher the inhibitory rate value, higher is the activity) than the corosolic acid. The corosolic acid analogs of this invention are found to show good ;5-lipoxygenase activity (Table 2; 5-lipoxygenase activity is expressed in % of inhibition at 100 µM and 250 µM; higher the % inhibitory values, higher is the activity). The corosolic acid analogs of this invention are found to show significant brine shrimp lethality (Table 3; brine shrimp lethaUty is expressed in LC50 at concentration; lower the LC50 value, higher is the activity). 2. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R2 is COCH3 and R1, R3, R4 are H, and R5 is COOH, which is 3-O-acetyl corosolic acid (1). 3. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R1 is COC5H4N, R2, R3 and R4 are H, and R5 is COOH, which is 2-0- nicotinoylcorosolic acid (3). 4. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R1 is COCH2NH2.HC1, R2, R3, R4 are H, and R5 is COOH, which is 2-0- glycylcorosolic acid hydrochloR1de (4). The corosolic acid analog of the formula I, as claimed in claim 1, wherein R] is COCH(CH3)NH2.HC1, R2, R3, R4 are H, and R5 is COOH, which is 2-0-alanylcorosolic acid hydrochloR1de (5). The corosolic acid analog of the formula I, as claimed in claim 1, wherein Rj is COCH=CHC6H2(OCH3)3, R2, R3, R4 are H, and R5 is COOCH3, which is methyl 2-O-(3,4,5-tR1methoxycinnamoyl)corosolate (6). The corosolic acid analog of the formula I, as claimed in claim 1, wherein R] and R2 are taken together to form S02, R3, R4 are H, and R5 is COOH, which is 2a3P”dihydroxyurs-12-en-28-oic acid 2,3-cyclicsulphate (7). The corosolic acid analog of the formula I, as claimed in claim 1, wherein Ru R2, R3 and R4 are H, and R5 is C0NH2, which is corosolamide (8). The corosolic acid analog of the formula I, as claimed in claim 1, wherein Rl5 R2, R3 and R4 are H, and R5 is CONHPh, which is N-phenylcorosolamide (9). The corosolic acid analog of the formula I, as claimed in claim 1, wherein R1? R2s R3 and R4 are H5 and R5 is CONHCH2CH2NH25 which is N-(2-aminoethyl)corosolamide (10). The corosolic acid analog of the formula I, as claimed in claim 1, wherein Rb R2, R3 and R4 are H, and R5 is CON(CH2CH2)2NH? which is N-corosolylpiperazine (11). 12. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R1? R2, R3 and R4 are H, and R5 is CONHCH2CH2OH3 which is N-(2- hydroxyethyl)corosolainide (12). 13. The corosolic acid analog of the formula I, as claimed in claim 1, wherein Rj and R2 are COCH3, R3 and R4 is H, and R5 is COOCH3, which is methyl diacetylcorosolate (14). 14. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R3 and R2 are H, R3 and R4 together form O, and R5 is COOCH3, which is methyl 11-ketocorosolate (15). 15. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R\ and R2 are COCH3, R3 and R4 together form O, and R5 is COOCH3, which is methyl diacetyl-11-ketocorosolate (16). 16. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R^ and R2 are H, R3 and R4 together form O, and R5 is COOH, which is 11- ketocorosolic acid (17). 17. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R] and R2 are COCH3, R3 and R4 together form O, and R5 is COOH, which is diacetyl-11-ketocorosolic acid (18). 18. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R1 and R2 together form SO2, R3 and R4 together form O, and R5 is COOH, which is 2a,3P-dihydroxyurs-12»en-ll-one-28-oic acid 2,3-cyclicsulphate (19). .. ■ 19. The corosolic acid analog of the formula I, as claimed in claim 1, wherein Rj and R2 are H, R3 and R4 together form O, and R5 is CQNH2, which is 11- ketocorosolamide (20). 20. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R1, R2 and R3 are H, R4 is OH, and R5 is CONH2s which is 11- hydroxycorosolamide (21). 21. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R1, R2 and R3 are H, R4 is OH, and R5 is COOCH3, which is methyl 11- hydroxycorosolate (22). 22. The corosolic acid analog of the formula I, as claimed in claim 1, wherein R1? R2, R3 and R4 are H, and R5 is CHO, which is corosolinal (24). 23. The corosolic acid analog of the formula I, as claimed in claim 1, wherein Rl3 R2, R3 and R4 are H, and R5 is COOCOC6H2(OCH3)3, which is corosolyl R1-O- methylgallate (25). 24. The corosolic acid analog of the formula I, as claimed in claim 1, wherein Rl5 R2 and R3 are H, and R4 and R5 together to form OCO, which is 2a,3b- dihydroxyurs-12-en-l 1,28-olide (26). 25. A process for producing corosolic acid analogs of the formula I compR1sing the steps of extracting leaves of Lagerstroemia species with water, alcohol or polar organic solvents, chromatographically puR1fying said extract to about 95% puR1ty and converting said corosolic acid to novel analogs by introducing new functional groups therein, in a known manner. 26. A pharmaceutical composition containing at least one corosolic acid analog as claimed in claims 1 to 24 and a pharmaceutically acceptable carR1er. 27. The pharmaceutical composition as claimed in claim 26 wherein said carR1er is an aqueous or non-aqueous carR1er. 28. A method of treating diabetes, inflammatory and tumor diseases compR1sing the steps of administeR1ng one or more corosolic acid analogs of the formula I.

Documents:

913-CHENP-2006 ABSTRACT.pdf

913-CHENP-2006 CLAIMS GRANTED.pdf

913-CHENP-2006 CORRESPONDENCE OTHERS.pdf

913-CHENP-2006 CORRESPONDENCE PO.pdf

913-CHENP-2006 FORM 18.pdf

913-CHENP-2006 FORM 3.pdf

913-CHENP-2006 PETITIONS.pdf

913-CHENP-2006 POWER OF ATTORNEY.pdf

913-chenp-2006-abstract.pdf

913-chenp-2006-claims.pdf

913-chenp-2006-correspondnece-others.pdf

913-chenp-2006-description(complete).pdf

913-chenp-2006-form 1.pdf

913-chenp-2006-form 3.pdf

913-chenp-2006-form 5.pdf

913-chenp-2006-pct.pdf

913-chenp-206.rtf