Title of Invention

"PROCESS FOR THE PREPARATION OF PHARMACEUTICAL PREPARATION CONTAINING NANOSOL"

Abstract

The present invention relates to a Process for the production of a pharmaceutical preparation comprising at least one pharmaceutically active substance such as herein described and a chitosan derivative, said pharmaceutical preparation being present in form of a dried nanosol, said pharmaceutically active substance being at least partially positively charged, and said chitosan derivative being negatively charged or a zwitterionic chitosan derivative, said process being characterized by selecting the chitosan derivative according to the type and relative number of its charged groups and according to the type and relative number of the charged groups of the pharmaceutically active substance such that at an isoionic state of charge equalization between the pharmaceutically active substance and the carrier can be achieved in the preparation at a pH value to reach the isoionic state results Preparing an aqueous sol from the negatively charged or zwitterionic chitosan derivative and the pharmaceutically active substance by adding the pharmaceutically active substance prior to or after dissolving the chitosan derivative or by uniting a solution of the chitosan derivative and an independently prepared solution of the pharmaceutically active substance; Adjusting the pH value of the aqueous sol such that an isoionic state results, with colloidal or nano-scale active substance particles precipitating; and Drying the thus-obtained aqueous nanosol.

Full Text

Pharmaceutical Preparation Pharmaceutical preparations wherein an active substance is present bound to a carrier are known in the state of the art in great abundance. In the widest sense, the bond to the carrier can be understood to be purely mechanical; in a narrow sense, however, one makes use of the capacity of carrier substances to enter into special chemical or physicochemical interactions with the active substance or substances. One category of such interactions are ionic attractive forces, which of course can only be made use of if active agent and carrier are at least partially present in a charged state. In pharmaceutical preparations, ionic bonds between active substances and carriers are used, inter alia, to preserve sparingly soluble active substances which have a low tendency of dissociation in water in their charged and molecular-disperse state, thereby obtaining a high dissolution rate.Apart from this, active agents are bonded to oppositely charged carrier polymers to enable a high active substance load of the preparation; this formulation technique is frequently used, for instance, in liposome jgregarations^. A further variant which has been described are preparations wherein by way of the ionic bond to a charged polymer it is intended to achieve a controlled release of active substance. An example for this is the cough mixture marketed in Germany under_the__mark Codipront® which contains as active substance carrier complex an active substance base, Codeine poly(styrene, divinyl benzene)sulfonate, bonded to an acidic ion exchanger. A special form of active agents bound to oppositely charged carriers are the so-called nanosols with gelatine or collagen hydrolysates as carriers/ which are described by the firm of Alfatec-Pharma GmbH in various patents and published applications, e.g., in the documents DE 41 40 195, DE 41 40 178 and DE 41 40 179_._Here, one makes use of the fact that it is easily possible to achieve the desired, isoionic state with charge equalization between carrier and active substance if gelatine or gelatine derivatives are used, thanks to the zwitterionic nature of the same, by means of a corresponding pH adjustment in the preparation. It is described that these nanosols can be used to advan¬tage for the production of medicinal preparations both with rapid and with controlled active substance release. However, these preparations have the disadvantage that the population has been uncertain for several years as to the possible risks of BSE infection and has increasingly been avoiding products containing gelatine, for example. Therefore, there is a need for preparations without gelatine or collagen derivatives which have the same advantages as, for example, the gelatine-based nanosols described. It is thus the object of the present invention to provide a pharmaceutical preparation without gelatine or the like, for charged active substances, in which the active substance is present bonded to an oppositely charged carrier. The object is achieved by a pharmaceutical preparation according to Claim 1. It was surprisingly found that using chitosans as carriers it is possible to produce so-called nanosols wherein the active substance is present stabilized in a state almost isoionic with the carrier, and that these nanosols are highly suitable for the production of medicinal products. The preparation of the present invention contains according to Claim 1 at least one pharmaceutical active substance, which is at least partial.ly_pregent in a charged state, i.e. the active substance_is capable of forming an ionic state and at least part of the active substance molecules are present in that ionic state. For a definition of a nanosol, referenre is made to DE 41 40 195. Considered as chitosan derivatives in the spirit ..of the invention are all modified and unmodified deacetylation products of chitin which still possess a polyglucosamine base structure. The charge opposite to that of the active substance, which is demanded according to the present invention, refers to the net-charge of the carrier used. Thus there may also be charges in the chitosan derivative that are like that of the active substance as long as they are overcompensated by the opposite charges. In fact, in one of the preferred embodiments there is an active substance with a postive charge that is bonded in the nanosol to a chitosan derivative with negative total charge. Such a chitosan derivative may, for example, be a zwitterionic, partially sulfated chitosan. In a further, also preferred, embodiment, the active substance is present in a negatively charged state and is bound in the nanosol to a positively charged chitosan derivative, i.e. in the most simple case to an unmodified chitosan. Here, too, an active substance may well be present in a partially undissociated form and may even possess some charges that are like that of the chitosan derivative as long as its net-charge is opposite, i.e. in this case negative. Preferably, the active substance is present in the nanosol in a colloidal or nanoparticulate distribution, i.e. with an average particle size of at maximum about 500-1000 nm, as far as it is possible to detect a phase boundary between active substance and carrier phase at all. In particular, poorly soluble active agents can be incorporated in this way in pharmaceutical preparations from which they can be quickly released. The preparation according to the present invention will as a rule contain further auxiliary agents which are commonly used in the pharmaceutics technology and are known to those skilled in the art. These active auxiliary agents may, for example, be further polymeric or non-polymeric carrier substances, but also stabilizers, surfactants, disintegration promoters, antioxidants, dyes, pigments, flavours, sweeteners or other taste-improving agents, binders, lubricants etc. In a preferred embodiment, the preparation contains a further polymeric carrier substance. This can be required, for example, in order to increase the loadability of the nanosol with active substance or in order to modify the release properties of the preparation. Appropriate formulation techniques are likewise known to those skilled in the art. In accordance with, the present invention it relates to a Process for the production of a solid pharmaceutical preparation comprising at least one pharmaceutically active substance that is capable of forming an ionic state and at least part of the active substance(s) molecule being present in an ionic state, which active substance is present in the form of a nanosol in which the active substance is bonded to an oppositely charged chitosan derivative, said process being characterized in that an aqueous solution is prepared from a negatively charged or zwitterionic, acidic chitosan derivative and a pharmaceutically active substance having positive charge by adding the pharmaceutically active sutostance prior or after dissolving the chitosan derivative or by uniting a solution of the chitosan derivative and an independently prepared solution of the pharmaceutically active substance, the chitosan derivative being selected according to the type and relative number of its charged groups and in coordination with the type and relative number of the positively charged groups of the active substance such that at a certain pH value an isolonic state or charged equalization between active substance and carrier is achieved in the preparation, the pH value of the aqueous sol is adjusted such that an isoinoic state results, possibly with colloidal or nano-scale active substance particles precipitating, and the thus-adjusted aqueous sol is dried. In accordance with the present invention it relates to Solid pharmaceutical preparation comprising at least one pharmaceutically active substance that is capable of forming an ionic state and at least part of the active substance(s) molecules being present in an ionic state, characterized in that the active substance is present in the form of a nanosol, said active substance having a positive charge and being bonded to a negatively charged or zwitterionic, acidic chitosan derivative. In accordance with the present invention, the herein disclosed pharmaceutical preparations are used for making medicinal products or diagnostic agents. A preferred use of the preparation consists in the production of medicinal agents which are administered as capsules, tablets, powders or granulates, or like instant preparations, are first dissolved or redispersed in water or another suitable liquid prior to being administered. In accordance with the invention, the herein disclosed pharmaceutical preparations are used for making medicinal products or diagnostic agents. A preferred use of the preparation consists in the production of medicinal agents which are administered as capsules, tablets, powders or granules, or like instant preparations, are first dissolved or redispered in water or another suitable liquid prior to being administered. In a further preferred embodiment, the preparations are used for preparing medicinal products having controlled active substance release. To this end, they must generally be further modified, i.e. mixed with further auxiliary substances or enclosed by these. For instance, capsules or tablets containing a preparation according to the present invention can be coated with a polymeric film which controls the release of the active agent or agents. These and further techniques for producing medicinal products with modified or controlled release of active substance are known to those skilled in the art. A preparation according to the present invention is basically produced in a multi-step process which can be varied if necessary or complemented by further steps. Initially, a chitosan derivative is selected as carrier, taking into account the relative number and type of the charged groups of the active agent, which on account of the type and relative number of its charged groups is matched with the active substance in such a way that at a certain pH value an isoionic state or charge equalization can be achieved between active substance and carrier. This is generally the case if the net-charges of active substance and chitosan derivative are opposite and the calculated isoionic point is in a pH range that is physiologically acceptable and is not detrimental to the stability of the active substance. In a further step, a colloidal aqueous solution is prepared from the chitosan derivative and the active substance, which on account of its polymer content and the viscosity resulting therefrom is a sol. It is of no importance here whether the active substance is added following or prior to dissolving the chitosan derivative, or whether a solution of the chitosan derivative and an independently prepared solution of the active substance are united. In a further step, the pH of the acjueous sol is adjusted * such that an isoionic state results. In the course of this pH shift a precipitation of the active substance may occur It has turned out here that the particles do generally not exceed the colloidal or nanoparticulate size range. The sol which has been thus prepared and adjusted to an isoionic state can be dried in a further process step. For this purpose, conventional drying methods, but preferably drying methods applying no or only little heat such as freeze drying, may be used. We claim: 1. Process for the production of a pharmaceutical preparation comprising at least one pharmaceutically active substance such as herein described and a chitosan derivative, said pharmaceutical preparation being present in form of a dried nanosol, said pharmaceutically active substance being at least partially positively charged, and said chitosan derivative being negatively charged or a zwitterionic chitosan derivative, said process being characterized by A} selecting the chitosan derivative according to the type and relative number of its charged groups and according to the type and relative number of the charged groups of the pharmaceutically active substance such that at an isoionic state of charge equalization between the pharmaceutically active substance and the carrier can be achieved in the preparation at a certain pH value. b) Preparing an aqueous sol from the negatively charged or zwitterionic chitosan derivative and the pharmaceutically active substance by adding the pharmaceutically active substance prior to or after dissolving the chitosan derivative or by uniting a solution of the chitosan derivative and an independently prepared solution of the pharmaceutically active substance; c) Adjusting the pH value of the aqueous sol such that an isoionic state results, with colloidal or nano-scale active substance particles precipitating; and d) Drying the thus-obtained aqueous nanosol. 2. Process as claimed in claim 1, wherein a further polymeric carrier substance is used in addition to the chitosan derivative. 3. Solid pharmaceutical preparation comprising at least one at least partially charged pharmaceutically active substance as described herein and a chitosan derivative, said preparation being present in the form of a dried nanosol in which the pharmaceutically active substance has a positive charge and is bonded to the chitosan derivative which is negatively charged or which is zwitterionic chitosan derivative, said solid pharmaceutical preparation being produced by a process, wherein - the chitosan derivative was selected according to the type and relative number of its charged groups and according to the type and relative number of the charged groups of the pharmaceutically active substance such that at a certain pH value an isoionic state or charge equalization between active substance and carrier can be achieved in the preparation; - an aqueous sol containing the active substance was prepared from the chitosan derivative, - the pH value of the aqueous sol was adjusted such that an isoionic state resulted, and with colloidal or nano-sclae active substance particles being precipitated; and - the thus-obtained aqueous nanosol being dried. 4. Solid pharmaceutical preparation as claimed in claim 3, wherein the active substance and the chitosan derivative are present in the nanosol in isoionic state. 5. Solid pharmaceutical preparation as claimed in claim 3, wherein the active substance is present in the nanosol in colloidal or in nanoparticulate form. 6. Solid pharmaceutical preparation as claimed in any one of claims 3 to 5, wherein it contains a further polymeric carrier substance in addition to said chitosan derivative. 7. Use of solid pharmaceutical preparations prepared by a process as claimed in any one of claims 1 or 2 or of a preparation as claimed in any one of claims 3 to 6 for the production of a medicinal product or a diagnostic agent. 8. A medicinal product produced by using a pharmaceutical preparation as claimed in any one of claims 3 to 6. 9. The medicinal product as claimed in claim 8, wherein it is a medicinal product for peroral application. 10. The medicinal product as claimed in claim 8, wherein it is a medicinal product that is administered as a powder, granulate, tablet or capsule. 11. The medicinal product as claimed in claim 8, wherein it is a medicinal product which is dissolvable or dispersible in a liquid. 12. The medicinal product as claimed in claim 8, wherein it has controlled active substance release. 13. A diagnostic agent produced using a pharmaceutical preparation according to any one of claims 1 to 6. 14. Process for the production of a solid pharmaceutical preparation substantially as herein described with reference to foregoing description and the accompanying drawings, tables and examples. 15. Solid pharmaceutical preparation substantially as herein described with reference to foregoing description and the accompanying drawings, tables and examples. 16. A medicinal product substantially as herein described with reference to foregoing description and the accompanying drawings, tables and examples. 17. Use of solid pharmaceutical preparations substantially as herein described with reference to foregoing description and the accompanying drawings, tables and examples.

Documents:

IN-PCT-2002-00187-DEL-PCT-304.pdf

in-pct-2002-187-del-abstract.pdf

in-pct-2002-187-del-assignment.pdf

in-pct-2002-187-del-claims.pdf

in-pct-2002-187-del-correspondence-others.pdf

in-pct-2002-187-del-correspondence-po.pdf

in-pct-2002-187-del-descriptioin (complete).pdf

in-pct-2002-187-del-form-1.pdf

in-pct-2002-187-del-form-13.pdf

in-pct-2002-187-del-form-19.pdf

in-pct-2002-187-del-form-2.pdf

in-pct-2002-187-del-form-3.pdf

in-pct-2002-187-del-form-5.pdf

in-pct-2002-187-del-gpa.pdf

in-pct-2002-187-del-pct-210.pdf

in-pct-2002-187-del-petition-137.pdf

in-pct-2002-187-del-petition-138.pdf