Title of Invention	GRANULAR EDIBLE PESTICIDAL COMPOSITION
Abstract	The invention provides a granular edible pesticidal composition comprising: (a) a continuous hydrophilic matrix phase comprising hydrophilic material, preferably in particulate form and water, said matrix phase being palatable to pests; and (b) a discontinous oleophilic phase dispersed within the hydrophilic matrix phase and comprising an oleophilic carrier and pesticide preferably dissolved in the oleophilic phase.

Title of Invention

GRANULAR EDIBLE PESTICIDAL COMPOSITION

Abstract

The invention provides a granular edible pesticidal composition comprising: (a) a continuous hydrophilic matrix phase comprising hydrophilic material, preferably in particulate form and water, said matrix phase being palatable to pests; and (b) a discontinous oleophilic phase dispersed within the hydrophilic matrix phase and comprising an oleophilic carrier and pesticide preferably dissolved in the oleophilic phase.

Full Text	Field of the Invention The present invention relates to granular pesticidal composition comprising: a) a continuous hydrophilic matrix phase comprising hydrophilic material, preferably in particulate fomi and water, said matrix phase being palatable to pests: and b) a discontinuous oleophilic phase dispersed within the hydrophilic matrix phase and comprising an oleophilic carrier and pesticide preferably dissolved in the oleophilic phase. Also, this patent related to formulations for the delivery of pesticidal agents and to methods for prepai-ing these fonnulations. In particular the invention relates to fomiulations which are edible and exert insecticidal activity when eaten by pests. Target pests can include any pest whose feeding activity has a deleterious influence on the activities of people, for example insects, spiders, mites nematodes, rodents. Background and Prior Art Edible pesticidal composhions have been widely used in control of pests. In such formulations the loss of active agent is a problem for the efficacy and environmental safety of the composition. The pesticidal agent is often liberated into the environment and is wasted (removed or destroyed) by processes such as volatilization, binding to clay or organic matter, microbial degradafion. chemical decay and leaching. This significantly reduces the effective life of the edible pesticidal fomulation. Another problem caused by edible pesticidaJ compositions is that the pesticidal agent is often toxic to beneficial organisms which prey on pests but do not cause feeding damage in their own right. The presence of residual sub-lethal quantities of pesticidal agents through loss of pesticide over time causes pesticide resistance to develop in the populadon of pests. This problem can be exacerbated by slow release formulations which generate significant zones or "'hot spots" of sub-lethal pesticide concentration. Sustained release formulations have been described which provide prolonged pesticidal activity by providing a slow continuous release of pesticide. Such sustained release formulations have been made by containing the pesticidal agent in a hydrophobic matrix material. Example of a controlled release formulatlorr Is the SuSCon ranye of controilea release chlorpyrifos granules sold by Cropcare Australasia Pty Ltd [of 77 Tingira Street. Pinkenba, Queensland, Australia] which are based on the use of thermoplastic resins (such as ethylene-vinyl acetate copolymers) as the matrix phase. Another example of a controlled release formulation is the aphicidal granule product based on the use of thennoplastic resins or wax as described in Australian Patent AU8944301 to ICI PLC. While the slow release of pesticides from these formulations increases the effective life of the edible pesticidal formulation it does not address problems of damage to non-target organisms or the built up of resistance. Many long-lasting hydrophobic matrix materials (e.g. ethylene vinyl acetate copolymers) are not edible by pests and so cannot be used to provide edible pesticidal form'jlations. Sustained release fom^ulations have also been made by containing the pesticidal agent in a hydrophilic matrix material (i.e. the hydrophilic material provides the continuous phase of the formulation). These hydrophilic materials contain a certain amount of water and may take up more wafer when they encounter wet conditions. Examples of pesticidal formulations which contain the pesticide in a hydrophilic matrix include: (1) The use of hydrated fibrous mats as carriers by Balassa in US patent (2) The use of thermoplastic hydrogels as carriers by Vaughan et al in Australian Patent AU07680991. (3) The use of reversibly dehydrated vegetable and/or fruit to provide rodent baits by Barth et al in EP 86107928 (4) The use of a carrier phase comprising milk solids and sucrose (in the presence of high levels of boric acid as active ingredient) by Nelson et al in US 6,153,181. Nelson points out that toxic baits for crawling insects have historically been water-based, and that water has been presumed necessary for good bait performance. Nelson explains that products result of water loss, rancidity, break-down of active ingredients etc. (5) The use of an aqueous plant fibre slurry (which is subsequently dried) as the matrix for an agricultural granule has been described by Lowe et al in US 5019564. Lowe et al note that the use of clay in the matrix can create chemical inactivation of active ingredients such as chlorpyriphos. (6) The use of polyvinyl alcohol and borate in water (subsequently dried) as a pesticide matrix has been described by Maglio in US Patent RE33. 670. (7) The use of portions of corncob In various ratios as a carrier for pesticides has been described by Katz et al in US 4563344. The discussion of the background to the invention herein is included to explain the context of the invention. This is not to be taken as an admission that any of the material referred to was published, known or part of the common general knowledge in Australia as at the priority date of any of the claims. None of the above formulations has been shown to provide long-term ingestible bait which properly contains active ingredient. Statement of the Invention The invention provides a granular edible pesticidal composition comprising: a) a continuous hydrophilic matrix phase comprising hydrophilic material, preferably in particulate form and water, said matrix phase being palatable to pests; and b) a discontinuous oleophilic phase dispersed within the hydrophilic matrix phase and comprising an oleophilic carrier and pesticide preferably dissolved in the oleophilic phase. Summary of the Invention It is surprising that the discontinuously dispersed oleophilic phase enhances the containment of the oil-soluble pesticide because the principal barrier to release would be expected to be die hydrophilic matrix. In one preferment the oleophilic phase is viscous at ambient temperature, i.e. the time taken to pour said oleophilic phase from a 100ml beaker is In excess of 10 seconds at 20'^C. and more preferably in excess of 30 seconds at 20'C. The oleophilic phase will preferably have a Brookfield viscosity greater than lOOcP, more preferably greater than 2a0cP. (Measured at a tennperature of 25°C.). The invention further provides a method of controlling ground dwelling pests in a region comprising applying the granular pesticidal composition as hereinbefore described adjacent or below the surface of the soil. In regions of thick vegetation the granules may be applied to the thatch of vegetatior^ adjacent the surface of the soil. In yet a further aspect the invention provides s method of preparing a granular pesticidal composition as hereinbefore described comprising: (i) mixing water with the hydrophilic phase to form a defonnable dough; (ii) spraying an oleophilic phase onto the hydrophilic phase and mixing; (iii) forming the mixture into granules; and (iv) drying the granules to mechanical integrity. Detailed Description of Preferred Embodiments The pesticidal composition of the invention comprises a discontinuous oleophilic phase which typically contains the pesticidal dissolved therein. Preferably the oleopt^ilic phase accelerates the rate of water lo-^« from thp matrix phase under the drying time test which will now be described. \r\ the drying time test water is added to the hydrophilic matrix phase to achieve 200 units of matrix phase at 60% moisture (i.e. water comprises 120 units thereoO-10 units of oleophilic material at 70'C are sprayed onto the matrix phase under agitation, and the mixiure is then pelletised (by exirjsion or compaction). If necessary starch powder may he added to the minimum amount required to ensure that the pellets retain their physical integrity. The pellets are placed in an oven at 70°C and the time taken to dry the granule from 60% to 10% moisture is noted. Suitable oleophilic materials are those in which the drying time (compared to the case when no oleophilic materia! i& added) is decreased by a factor of 20% or greater. It Is surprising that the use of an oieophillc material which accelerates the rate of water loss from a water-swollen hydrophilic matrix phase can lead to enhanced containment of the oil-soluble pesticide. The composition of the invention comprises a continuous hydrophilic matrix phase comprising hydrophilic material and water. The hydrophilic material is typicaily a particulate solid. Preferably these particulate entities comprise eccentric particles with a ratio of maximum dimension to minimum dimension of at least 2, more preferably at least 5. Even more preferably the hydrophilic entities comprise fibres or fibre segments of length 0.05mm or more, most preferably 0.5mm or more. The continuous hydrophilic phase contains water. The water may be present in amounts of at least 0.6% by weight of the hydrophilic phase and is preferably present in amounts of at least 5% by weight of the hydrophilic phase. The preferred upper limit foi watei will generally be governed by the desired mechanical integrity of the composition. Typically no more than 30% by weight. In one preferment the hydrophilic matrix phase, when swollen with water, can be formed into a deformable dough ur'^or the action of hi?h prcsswre s'i^;aii;;y forces. The hydrophilic matrix phase 's edible to target pests. The hydrophilic matrix may comprise a wide range of organic materials aiyiough decomposed plant material and plant fibres is particularly preferred. The hydrophilic matrix may comprise edibie material such as stable composted material, plant fibre, plant husks, raw or process cereal, blood and bone, bone meal, peat, animal manure and mixtures thereof. In one particular prefenTie:;; the hydrophilic rnatrix comprises peat or com fibre, preferably wood peat or reed sedge peat or sphagnum peat. More preferably fibrous reed sedge peat Most preferably the hydrophilic matrix comprises a fibrous peat. In one preferment the hydrophilic matrix phase has a buffer capacity such that the inside of the granule (under prolonged soil storage conditions) can be maintained at 1 or more pH units different from the surrounding soil, preferably 2 or more units. In one preferment the Inside of the granule has a neutral or acid pH value. This neutral or acid pH is preferably maintained even when the surrounding soil is at pH 8.5 or greater. The oleophilic phase will generally contain the pesticide as a minor component on a weight bases. The amount of pesticide will thus nom-tally.lje iess.thaa 505:^ by weight of the oleophilic phase. More preferably the amount of pesticide is no mpre than 40% by weight of the oleophilic phase. The amount of pesticide is most preferably from 0.001 to 33% by weight of the oleophilic phase. In a particularly preferred embodiment of the invention the carrier of the oleophilic phase comprises chlorinated hydrogen, preferably containing at least 8 carbon atoms, more preferably at least 12 carbon atoms and more preferably from 12 to 20 carbon atoms. The degree of chlorinatlon of the wax is preferably 40% c ^roqtftr^ more prefersbly the nhic'irialT?':" VHA is Ceieclor AS52 sow by Orica Australia Pty Ltd of Melbourne Australia, The chlorinated hydrocarbons are in the form of viscous oils or waxes The granules preferably retain their morphology in soil over a 1-3 year period which includes numerous wet/dry cycles. Preferably the granules do not kill pests except by Ingestion. In particular it is prefen-ed that the granules do not act as a contact poison, even if the pesticidal agent is a contact poison. Preferably when the granules are in close contact with large inEscts such as cockchafers or whitegrubs they are not injurious. Preferably the granules do not deposit peslicidal concentrations of pesticide in the region proximal to the granule. If the granules are located proximal to a vertical porous membrane in a sub-soil environment, pests on the other side of the porous membrane are not injured, even if the porous membrane is permeable to the pesticide. The pesticide w/hich is present in the composition of the invention Is preferably an oil soluble pesticide, more preferably a volatile, oil soluble pesticide and more preferabty an organophosphate such as chlorpyrifos. Preferably at least 50% by weight of the pesticidal agent remains contained within the granule after 3 months of residence in soil, more preferably at least 50% by weight after 6 months and more preferably 80% by weight. The invention Is most suited to using insecticides which have a validity which provides a vapour pressure of at least one miltipascal as measured oy ASTM D5191. The composition of the invention is particularly suited to control of soil and thatch dwelling pests. These pests include members of the Classes; insects (Class InSGcta) , nematodes (Phylum NematodaJ, mites (Class Arachnida, Sub-Class Acari), spiders (Class Acarina. Order Araneae), slugs and snails (Class Gastropoda), Millipedes (Class Diplopoda), springtails (Cass Collembola). symphylids (Class Symphyla). Th.9 nrgnijtoc will tvp!,-'.;y bc placed on or ueneai'ti ihe surface or the scii or where thick vegetation is present, they may be p'aced In the thatch covering adjacent the surface of the soil, The procedures which will typically be used for placing the granules may include cultivation of soil on which granules are placed or injecting or drilling the granules into the soil or vegetation thatch. In one preferment the hydrophilic matrix phase including water makes up 60 -50% more preferably 80 - 95% and most preferably 80 - 90% by weight of the total composition. The oleophilic phase typically comprises 1 - 25% by weight, preferably 5 - 25% by weight (based on the weight or ihe oleophilic phase) of pesticide, such as chlorpyrlfos, preferably in chlorinated wax. The whole oleophilic phase preferably mates up 5 - 40% more preferably 5 - 30% and most preferably 5 - 20% by weight of the total weight of the composition. In the case of peat the hydrophilic matrix phase is typically dried to provide a water content in the finished product of up to 30% wAv and more preferably 5-25% w/w. In one specific example the pesticidal composition contains 9% w/w Cerachlor, 1% chlorpyrlfos w/w, 20% w/w water and about 70% w/w peat (on dry weight basis). We have successfully made baits using an oil phase as high as 20% w/w of the total final product. There is disclosed a method for mal (1) adding water to the hydrophilic phase until the phase can be fornied into a deformable dough under pressure; (2) spraying oleophilic phase onto the hydrophilic phase and mixing, for example in a rotary drum; (3) forming the mixture into granules for example by a method selected from extrusion, compaction granulation, basket granulation or other methods known to the art; and (4) drying the granules. Preferably the extruder achieves a compaction ratio of at (east 1.5, preferably at least 2. Preferably a single screw front plate exlruder is used to form the granules. Preferably inner and outer cutter blades are placed proximally to the extruder die plate. The inner cutlet blades cut fibres which bridge between orifices in the front plate. The outer cutter blades cut the extruded rods Into granules. We have found that the use of internal cutting blades is vety important with peat and compost and other long fibred rnateria! but less so with material such as blood and bone. With peat and fibrous materials the outer cutting blades are less necessary than the inner cutting blades. The outer cutters are used to get the bait granules to the desired length hut this can be achieved, through other means such as in a rolling drum. Treferablyjhe granulesare. dried w^the-grarTqtera£hieve'ari indivtdUall crush - --- ——-"^ - - strength of at le^sl 500g and more preferably at leasHOOOg. The simultaneous use of both inner and outer moving cutter blades proximalty to the extruder die piate is believed to be a novel feature of the pelletisation process. Throughout the description and ciaims of this specification, the word "comprise" and variations of the word such as "comprising" and "comprises", is not intended to exclude other additives or components or integers, The Invention wit) now be described with reference to the following examples. It is to be understood that the examples are provided by way of illustration of the invention and that they are in no way limiting to ^e scope of the invention. Examples Example 1: Formulation of inseciicidaJ chhrpyrifos baits Fibrous reed sedge peat (60% moisture) was put thiough a thresher to provide size comminution, leaving the peat as a loose particulate mass comprising fibres aenerally less than 5mm Innn The peat was taken from the Peat Operations mine on Tinengower property, Swan Marsh Irrewillipe road, Swan Marsh District. Colac, Victoria Australia. The fibrous peat generally is located in the upper 600mmm of the resource. 1.2g of chlorpyrifos was heated to 50C and dissolved in 9g of chlorinated paraffin wax at 50°C. The chlorinated wax was a C14 wax and was 52% chlorinated. This material is sold undef the trade name Cereclor AS52 by Onca Australia Pty Ltd of Melbourne Australia. The oleophilic wax phase (10g) was sprayed onto 197g of fibrous reed sedge pest phase (60% moisture) under agitation by a rotary stirrer. The dough was extruded through a Moulimex single screw front plate extruder to form granules of dimension 3mm dtameter x 6mm length. The granules were dried in a fan-forced oven at 70°C for 5 hours. The final granules were measured to contain 1% chlorpyrifos and 20% water. Example 2: Chlorinated wax accelerates the rate of water loss from fibrous reed sedge peat granules at 60% moisture. ExtnJded granules were made according to the process of example 1 but without the drying step and without the use of chlorpyrifos. Granules designated G1 were made without the addition of chlorinated wax, and granules designated G2 were made with the addition of 9 grams chlorinated wax. 5g samples of granules were placed onto 10cm diameter aluminum foil dishes and placed in a fan forced oven at 70°C. The dishes were weighed at regular intervals after being placed iri the oven. The time taken for G1 granules to reach 10% water content was 125 minutes, and the time taken for G2 granules to reach 10% water content was 40 minutes. Example 3: Peat Granules are edible to greybaci (Denvoiepida albohirtum) Eggs taid by adult beptles were colSe.cterf arid the f!rs» instar g.-bc v;cr^ s'-'ovv-w to hatch. Each of 15 replicates consisted of a single grub which was placed in moist sand in a 70ml vial. The newly hatched cane grubs burrowed into the sand, and then a black reed sedge peat pellet (containing no oleophilic inclusions or chlorpyrifos) was placed on top of the sand surface. The trial continued for 7 days, Examination of peat pellets showed eroded segments with bite marks which could only be explained by larval feeding. Examination of grUDs (the gut .regions were transparent) showed black peat fragments in their gut. Example 4: 1% cNorpyrifos peat bait made accoraing to the inwniion cfves not kill wf^ite j/rujbs by contact activity 1% chlorpyrifos granules were made according to tfie method of example 1, When ingested, these granules provided 100% mortality of Z"^ instar cockchafer larvae, however when the granules were adhered to the front of 3" instar lar^'ae using surgical adhesive tape, the mortality was not significantly different from controls. Example 5: 1% chlorpyrifos baits made according to ttiis invention properly contain the chlorpyrifos within the bait 1% chlorpyrifos granules were made according to the process of example 1. Granules designated G2 were made according to the Invention using an oleophiiic phase comprising 1 part chlorpyrifos dissolved in 9 parts chlorinated wax. Granules designated G3 were made by adding neat chlorpyrifos to the hydrophilic phase (no additional oleophilic material was included). Granules wore buried 1 cm into aiicaline Wimmera clay (pH 8.5) from the Wimmera region of Victoria Australia, which had been remoistened with water (to 25% water) and placed Into 500ml tins with approximately 2cm of air space at the top. The tins were sealed and placed into an oven at 35°C. After 3 months incubation at 35^0, granules designated G2 were found to contain 85% of the chlorpyrifos originally present. Granules designate G3 were found to contain only 20% of the chlorpyrifos originally present Example 6; This example demonstrates the control of a range of ground dwelling pests using components of the invention. Example, 6a: Chlorpyrifos baits made according to this invention were us&d to control cane grub larvae in laboratory tests First instar cane grub larvae {Dermolepida albohirtum) were used as test individuals. The test was conducted at 25°C. Peat granules were made according to the method of example 1, but with varying amounts of chlorpyrifos. in one treatment the reed sedge peat was replaced with humic peat, a peal from the same mine but which contains far less organic matter. (See Table 2). soma treatments (see Table 2), and was placed below the bait granule so that the (arvae would have to pass the attemative food source to find the bait. At 5 days after treatment (DAT) there were no dead gaibs in the control, however at least 80% of goibs had died In all treatments containing granules with chlorpyrtfos concentrations of 0.05% or more. Of the treatments containing 1% chlorpyrtfos, all had 100% grub mortality. By 7 DAT, all chlorpyrifos granules had led to 100% grub mortality, although no grubs had died in the control. Example 6b: Chlorpyrifos baits made according to this invention were used to control white grub larvae (Acrossidus tasmaniae) in laboratory tests Two concentrations of chlorpyrifos, 0.1% and 1%, were used to prepare granule samples as per Example 1 and another sample of granules was made omitting the chlorpyrifos and is designated as Control + Peat. The protocol for evaluation of bioefficacy on gnjbs was the same as for Dermoiepida a}bohirtum in Example 6a, 15% of grubs in the Control and 25% in the Control + Peat treatment died during the experiment. By comparison 100% of gnjbs were dead within 2d for the 1% granules and within 4d for the 0.1% granules (see Table 3). Examph 6c; Chhrpyrifos baits made according to this invention were used to control an alternative canegnjb species (Lepidiota negatona) in iaboratory tests Granules were prepared as per Example 6b. Or.^ sample of granules was made omitting the chlorpyrifos and is designated as Control + Peat, The protocol for the evaluation of tjloefficacy on grubs was the same as for Dermolepida albotiirtum in Example 6a, Mortality of crickets for the treatment receiving the bait was 100% while in the untreated controls it was 0%. Exttmple 7. ManufactUie of a batch of poiticida! granules Threshed reed sedge peat (19.7kg, 60% moisture content) was placed in a rotating drum cement mixer (0.8m max diameter tapered drum leading to 0.4m orifice). 120g of chlorpyrifos was dissolved in 900g Cereclor AS52 chlorinated wax at 50°C under agitation. This oleophilic phase was drip fed into the reed sedge peat under agitation in the cement mixer over a 5 minute period, and the mixer was kept running for a further 5 minute period. The contents of the cement mixer were processed in a Fabio Leonard! 0.7 HP front plate extruder. (Fabio Leonard! are based In Bo, Italy). The front plate was 8cm in diameter and the holes were 3mm in diameter. The extruder used a variable-pitch single screw providing a compression ratio of 2:1. The extruder was purchased with a fitted internal cutter mounted to the screw. An externa! cutter comprising a sharp steel blade was joined to an electric drill bit and was rotated independently of the extruder screw. The cutter was located on the external face of the die and was used to chop the extruded strands into granules. The extemal cutter was rotated in a reverse sense to the rotation of the extruder screw. The resultant granules (3nrim diameter x 5mm length) were dried in a drum drier Im .-"- r.r;ri ZOc-r. Jistp, and ihs exterior or tne steel drum was directly heated by a gas flame. The granules were dried to 15% moisture and were measured to contain 1% chlorpyrifos by weight gxampie 8: Failure of conventional methods to produce robust fibrous reed sedge peat granules Fibrous reed sedge peat (60% moisture) was added to a Fuji Paudat model EXDTF100 extruder with a 3mm die. The trial extrusion took place at the laboratories of Fuji Paudal in Osaka, Japan on Sept 11 & 12. 2000. After 36 seconds of operation, the die became blocked as the peat fibres were laid flat against the internal face of the die by the motion of the screw. Fibrous reed sedge peat was added to a range of commercial pellet mills which utidsed a rotating kneading action to force the peat through s die. All these pellatisers became blocked within 10 minutes of operation. Furthermore the granules (before blockage occun-ed) were not homogenous and compact but were striated In morphology (reflecting the action of multiple pressure pulses in the pelletiser). The granules thus produced snapped readily and were not robust enough for application to and stable residence in soil. Example 9: Field Trial - Control of cane grub Sugar cane was planted at hvc sites in July 2000. IK Octobei 2000, 10m x 6m plots comprising four planted rows were laid out and 0.5% chlorpyrtfos granules made according to the method of Example 7 but with half quantities of chlorpyrifos and chlorinated wax were broadcast by hand at a rate equivalent to 250kg per hectare. The granules were incorporated to a depth of I5cm using a power harrow. Flights of adult greyback cane beetles {Derrnolepida albottirtum) took place in December 2000 - January 2001, and in February four cane stools per plot were dug up from the two middle rows and the number of grubs per stool was counted. Each treatment was replicated 5 times, i.e. a total of 20 stools were dug up for each treatment. Ths rf>Rriit« vA/(?re e.>:p."accc:d in t"rm= o' the average number of grubs per stool (see Table 5). At a third site a similar experiment was lard out in a sugar cane ratoon (re-growth from cane cut in previous season). Trials were carried out at site 1 (Farmer Romeo, Burdekin District, Queensland, Australia), site 2 (ratoon crop, Farmer Sgaibossa, Burdekin Districl. Queensland, Australia) and site 3 (Farmer Marciilio. Tully District, Queensland, Australia). Sxampie 12: 3mm b&its made according to the invention from a number of Insecticide ct^smical groups are storage stable A sample of 3 mm diar. igter baft granutes wa? made from each of the active ingredients according to Example 1 except that chlorpyrifos was replaced with one of the active ingredients in the table below and for imidachfoprid 0.12 g of imidachloprid was substituted for 1.2g of chlorpyrifos. lOOg samples of each of these granules were taken arid divided into four. 25g duplicate samples were placed into a 50ml, sealed glass vials and placed in an oven to be kept at 54'C for 14d. The other two duplicate samples were analysed for active ingredient The samples taken from the oven after I4d were also then analysed for the active Ingredient content. This test not only evaluates the storage stability of the granules but also estimates the relative loss of active ingredient when in the soil for a prolonged period. When an oleophilic phase was present all active ingredients were found to be stable during 14d of storage at 54°C with none degrading by more than 8% (see Table 7), evaluation of bioefficacy on grubs was the same as for Example 6b except that mortality was measured on second - third instar grubs rather than new hatched grubs and at 3d after treatment. Example 14: Baits made from other actives according to this invention control termites (Coptotermes acinaciformis) in laboratory tests Samples of granules were prepared as per Example 13 using bifenthrin, carbaryl. diazinon, methidithion and trichlofon plus a sample was made up containing 1% chlorpyrifos granules . "Xhe protocol for the evaluation of bioefficacy on termites was the same as for Example 13 except that 5 Teplicates each of 5 worker caste termite individual were used. No termites in the Control died during the experiment. By comparison the mcriclitj in tr;,ct,.fius contalnlriy in^BuilcIdes ranged from TO -yb'fo {zee Tsbie 9). Example 16: Chlorinated wax reduced chlorpyrifos losses from a wide range of edible, hydrophjiic matrices The hydrophiiic matrix miist br edihle tc soil borne pests and campatible '/.-iih the oleophilic phase and active ingredient and retain the active ingredient during the drying stage of manufacture and iater when in the soil for prolonged periods. An elevated temperature tests can be used to estimate the relative losses under these conditions and so the relative suitability of these matrices, Water was sprayed onto 500g of each matrix (see Table 12), while continually stirring, until a small amount could be extruded successfully through a Moulimex single screw front plate extruder to form granules 3mm in diameter to form the "pre-wet matrix". The chicken manure matrix was made by breaking up Yates' Dynamic Lifter chicken manuie pellets using a mortar and pestle prior io adding the water. The moisture content of each "pre-wet" matrix was then determined. One part chlorpyrifos was then added to 9 pari:s Cerachlor AS52 to form the "liquid blend A" or alternatively one part chlorpyrifos was then added to 9 parts acetone to form "liquid blend B". The moisture content of the matrix was then used to calculate the amount of either "liquid blend A or B" that was required to be added to the "pre-wet matrix" to result in approximately 1% chlorpyrifos after granules weie dried for 3h at 7Q°C. The relevant estimated amount of "liquid blend" was then added to the "pre-wet matrix". This sample was then passed through the extruder to form granules. These granules were spread thinly onto a stainless steel tray and placed into a fan forced oven at 54'C for 2 weeks. The granules were then removed and their chlo[pyrifos content determined. Example 17: Oleophilic phase reduces chlorpyrifos losses under a temperature challenge The oleophilic phase must be able to act as a scivent (or at Inast be miscible) with the active ingredient and reduce the loss of the active ingredient during the drying stage of manufacture and later when in the soil for prolong periods. An elevated temperature tests can be used to estimate the relative losses under these conditions and so the relative suitability of the oleophilic phase candidates. Under this test suitable candidates will reduce losses to one third of that of active ingredient alone. 1.2g of chlorpyrifos was added to 9g of each alternative oleophilic candidate (see Table 14). 1,02g of this liquid blend was then placed onto aluminium foil within a glass Petrie dish. The control was molten chlorpyrifos alone. Samples were then placed in a fan forced oven at TCC. for 70h before being removed and analysed for chlorpyrifos content. Example IS: Alteniativv nieophilic phases reduce chlorpyrifos losses undei a tamperature challenge 1% chlorpyrifos granules were made according to the process of Example 1 with the exception that the Cerachlor AS52 was substituted for 5 of the other candidates in Table 14. Each granule sample was divided into two sub-samples and one was placed into a fan forced oven at 54C for 14d and the other was analysed for chlorpyrifos content When the sub-samples were taken from the oven they too were analysed for chlorpyrifos. None of these candidates performed particularly well in this test hut chnrter chain chlorinated paraffins with greater chlorination make the best oleophilic phase for chlorpyrifos (see Table 16). Example 19: 1mm diameter and 7mm diameter baits made according to the Invention using the organophosphaie chloipyrffos are storage stabiB Four samples of granules were made. One sample of granules were made according to Example 1. the second sample was made using the same method except the granules had a diameter of 7mm. The third and fourth sample were made according to Example 1 except that >n_«iune was substituted for the oleophilic phase. Sample 3 had a diameter of 3mm and Sample 4 had a diameter of 7mm. lOOg sub-samples of each of these granules were taken and divided into Tour. Duplicate 25g samples were placed into a 50ml, sealed glass vial and placed in an oven to be kept at 54'*C for 14d. The other duplicate samples were analysed for active ingredient. The samples taken from the oven after 14d were also analysed for the active ingredient content. In the absence of the oleophilic phase 45% of the chlorpyrifos was lost from 7mm granules during 140 of storage at 54°C compared with 1"!% when the oleophilic phase was present (see Table 17). The hydrophilic matrix must be edible to target pests. Granules were made up from 11 hydrophilic matrices (see Table 19) as per Example 16, with the sxceptiL., th:it ='! i_.,"r-:jl£S v;er£ made up vvit!'- OT;=:L;!:i'jr AS52 and none with acetone. Bioassays Were undertaken on white grubs (Acrossidus tasmaniae) as per Example 6b and on termites (Copteterwes acinaciformis) as per Example 14- Example 21: 1% chhrpyhfos baits made according to this invention properly contain chlorpynfos witliin baits made from a range of edible, hydrophilic matrices The edible hydrophilic matrix must be able to rciain the active- ingrGrtient not just during the drying stage of manufacture but also later when in the soil for prolonged periods. An accelerated loss test can be used to estimate the relative losses in soil. ©ranulQS were made as for Example 5 except that the hydrophilic matrix was Austrslian iignin peat Irom Eco-Gro International, Wlallanda, Queensland, Australia. These granules are designated as G3. Whereas reed sedge peat has been formed by the degradation of reed sedge, Iignin peat has resulted from the degradation of wood and trees, and djffeis in composition. Lignin peat is also called wood peat or woody peat. This data has been tabled against the data from Example 5. After 6 months of storage at 35°C there was littte difference in the rate of chlorpyrifos losses from G2 or G3 granules from these two peats that have very different origins and compositions (see Table 24). Examp/g 23. B&Hs made Bccording to this invention properfy contained a number of aitemative active ingredients when placed into the soil af elevated temperature tt has been shown previously that the Invention can oroperly r^^tain chiorpyrifbs in the hydrophilic matrix when stored in soil. In this example other active Ingredients are also shown to be held within a reed sedga peat matrix. A 3mm diameter bait was made as pre Example 1 except that the chlorpyrifos was replaced with bifenthrin (synthetic pyrethroid), carbaryl (carbamate), methidathion (organophosphate) and endosulfan (organchlorine). The granules were placed into soil at elevated temperature as per Example 5 for one month. After one month in the soil more than 50% of the originai active ingredient was found to remain in the granules. This would be far more than that required to control pests (see Table 25). Example 24: Granules made at two ratios of active ingredient to ail phase Qccofdfng to (his invention property contained the active ingredient when placed into the soil at elevated temperature tt has been shown previously that the invention can properly retain chlorpyrifos in the hydrophilic matrix when made in a ratio of Iparl chlorpyrifos to 9 parts oil phase. In this experiment the ratio of 1 part chlorpyrifos : 3 parts oil phase was used in a 5% chlorpyrifos granules and 1 part chlorpyrifos: 99 parts oil phase in a D.1% granules. Granules were niade as pre Exampie 1 except that either Sg.of chlorpyrifos was added to ISg of Cerachlor AS52 and sufficient added to make 5% chlorpyrifos granules or O.lg chlorpyrifos was added to 9g Cerachlor AS52 and sufficient added to make 0.1% chlorpyrifos granules. The granules were placed into soil at elevated temperature as per Example 5 for one month. After one month in the soil more ttian 93% of the original chlorpyrifos was found to remain in the 5% granules while 96% was found in the O.T % granules. Example 25:_ The solubility of (he active ingredient in the olf^nnhiHc phzz^ effects the performance of (his phase. It was found that the preferred oleophilic phases provided a solubility for the pesticide of at least 20% w/w, more preferably at least 30% w/w and still more preferably at least 40% by weight based on the combined weight of oleophilic phase and pesticide. The solubility of chlorpyrlfos was determined for the oleophilic phase candidates in Example 17. It was found that preferred candidates could contain 20% w/w of chlorpyrlfos in the weight of chlorpyrrfos plus oleophilic phase and preferably 30% and more preferably 40%. Example 26: 1mm diameter baits made according to the invention using alternative pesticides ere storage stable 1mm diameter samples were made and treated according to Example 19a except that the chlorpryifos was replaced with the alternative pesticides, diazinon or terbufos. In the absence of the oleophilic phase a large proportion of these volatile pesticides were lost during bolh the drying phase and the storage phase. In the absence of the oleophilic phase approximately 30% of terbufos was lost during drying and then, based on the terbufos content of the dried granules, P further 70% was lost during storage rn soil. In the p^essnce of the oleopniiic phase this reduced to approximately 18% and 48% respectively (see Table 26). In the absence of the oleophilic phase approximately 6% of diazinon was lost during drying and Ihen, based on the diazinon content of the dried granules another 64% was lost during 14d storage in soil. In the presence of the oleophillic phase this reduced to approximstely 0% and 49% respectively. We Claim: 1. A granular odibic pGSticidal composition comprising: (a) a hydrophilic matrix comprising a hydrophillc material and vvak'r, said matrix being palatable to pests; and (b) a discontinuous oleophilic phase dispersed within the hvdrophlic matrix and comprising an oleophilic carrier and a pesticide; wherein said composition comprising 70 to 95% by weight of the h\ drophilic matrix and 5 to 30% by weight of the oleophilic phase and ha\'inj', an individual crush strength of atleast 500g. 2. 'I'hc granular pcsticidal composition as claimed in claim 1 wherein, following storage at 35°C for 12 months in alkaline Wimmera clay {pi i 8,5) which has been rcmoistcned with water (to 28%), the composition retains at least 70% ol the quantity of the pesticide present in the composition prior to said storage. 3. The granular pesticidal composihon as claimed in claim 1 or claim 2, wherein the hydrophilic matrix in the composition prior to said target pests. 4. The granular pcsticidal composition as claimed in any one of claims I to 3, wherein the hydrophilic material is in particulate form. 5. The granular pcsticidal composition as claimed in any one of claims 1 to -1, wherein the pesticide is dissolycd in the oleophilic phase. 6. The granular pcsticidal composition as claimed in any one of claims 1 t(^ 5, wherein the amount of pesticide is less than 50% by weight of the oleophilic phase. 7. The granular pesticidal composition as claimed in any one of claims 1 to 6, wherein the discontinuous oleophilic phase enhances the containment o( the pesticide. 8. The granular pesticidal composition as claimed in anv one of claims 1 lo 7 comprising from 0.001 to 33% by weight, based on the weight of the oleophilic phase, of the pesticide. 9. t'he granular pesticidal composition as claimed in any one of claims 1 to S comprising from 5 to 20% by weight of the total composition o( the oleophilic phase and HO to 95% bv weight of the total composition of the hydropiiiiic matrix. 10. The granular pesticidal composition as claimed in any one of claims 1 lo 9, wherein the oleophilic phase has a Brookfield viscosity at a temperature of 25" C greater than lOOcP. 11. The granular pesticidal composition as claimed in claim 10, when^in the oleophilic phase comprises one or more oleophilic carriers selected from the group consisting of chlorinated hydrocarbons and polyalkylene glycols, 12. The granular pesticidal composition as claimed in any one of claim 10 or claim 11, wherein the oleophilic phase comprises a chlorinated hydrocarbon comprising atleast 12 carbon atoms and having a degree of chlorination of atleast 40%. 13. The granular pesticidal composition as claimed in any one of claims 1 lo 12, wherein the hydrophilic material is selected from the group consisting of processed or unprocessed cereal grains, blood and bone, peal ami animal manure, 14. The granular pesticida! composition as claimed in any one of claims 1 io 13, wherein the hydrophiUc material is peat. 15. The granular pesticidal composition as claimed in any one of claims 1 to 14, wherein the pesticide has a vapour pressureof atleast one milipascal, 16. I'hc granular pesticide as claimed in any one of claims 1 to 15, wherein the pesticide is selected from the group consisting of organophosphale, insecticides, organochlorinc insecticides, carbamate insecticides, synthetic pvrethroids, guanidinc/neonicotinoids and mixtures thereof. 17. Ihe granular pesticidal composition as claimed in claim 16, wherein the pesticide is an organophosphale. 18. 'J'he granular pesticida! composition as claimed in any one o( claims 1 to 17, wherein the oleophilic phase provides a drying time (compared to tfie case when no oleophilic material is added) which is decreased bv a factor of 2(1% or greater. 19. The granular pesticidal composition as claimed in any one oi claims 1 to 18, wherein granules of the composition have a maximum dimension in the range of from 0.5 to 10mm. 20- The granular pesticidal composition as claimed in any one of claims 1 to 19, wherein granules of the composition are extruded. 21. The granular pesticidal composition as claimed in any one of claims 1 to 20, wherein the hydrophilic matrix has a buffer capacity under prolonj'ed sLoraj',e conditions. 22. Ihe granular pesticidal composition as claimed in anv one of claims 1 to 21, wherein the granules maintain their morphology in soil over a 1-3 vear period, 23. 'i'he granular pesticidal composition as claimed any one of claim.s 1 io 22, wherein tlie granules do not act as a contact poison.

Full Text

Field of the Invention
The present invention relates to granular pesticidal composition comprising: a) a continuous hydrophilic matrix phase comprising hydrophilic material, preferably in particulate fomi and water, said matrix phase being palatable to pests: and b) a discontinuous oleophilic phase dispersed within the hydrophilic matrix phase and comprising an oleophilic carrier and pesticide preferably dissolved in the oleophilic phase. Also, this patent related to formulations for the delivery of pesticidal agents and to methods for prepai-ing these fonnulations. In particular the invention relates to fomiulations which are edible and exert insecticidal activity when eaten by pests.
Target pests can include any pest whose feeding activity has a deleterious influence on the activities of people, for example insects, spiders, mites nematodes, rodents.
Background and Prior Art
Edible pesticidal composhions have been widely used in control of pests. In such formulations the loss of active agent is a problem for the efficacy and environmental safety of the composition.
The pesticidal agent is often liberated into the environment and is wasted (removed or destroyed) by processes such as volatilization, binding to clay or organic matter, microbial degradafion. chemical decay and leaching. This significantly reduces the effective life of the edible pesticidal fomulation.
Another problem caused by edible pesticidaJ compositions is that the pesticidal agent is often toxic to beneficial organisms which prey on pests but do not cause feeding damage in their own right.
The presence of residual sub-lethal quantities of pesticidal agents through loss of pesticide over time causes pesticide resistance to develop in the populadon of pests. This problem can be exacerbated by slow release formulations which generate significant zones or "'hot spots" of sub-lethal pesticide concentration.
Sustained release formulations have been described which provide prolonged pesticidal activity by providing a slow continuous release of pesticide. Such

sustained release formulations have been made by containing the pesticidal agent in a hydrophobic matrix material.
Example of a controlled release formulatlorr Is the SuSCon ranye of controilea release chlorpyrifos granules sold by Cropcare Australasia Pty Ltd [of 77 Tingira Street. Pinkenba, Queensland, Australia] which are based on the use of thermoplastic resins (such as ethylene-vinyl acetate copolymers) as the matrix phase.
Another example of a controlled release formulation is the aphicidal granule product based on the use of thennoplastic resins or wax as described in Australian Patent AU8944301 to ICI PLC.
While the slow release of pesticides from these formulations increases the effective life of the edible pesticidal formulation it does not address problems of damage to non-target organisms or the built up of resistance. Many long-lasting hydrophobic matrix materials (e.g. ethylene vinyl acetate copolymers) are not edible by pests and so cannot be used to provide edible pesticidal form'jlations.
Sustained release fom^ulations have also been made by containing the pesticidal agent in a hydrophilic matrix material (i.e. the hydrophilic material provides the continuous phase of the formulation). These hydrophilic materials contain a certain amount of water and may take up more wafer when they encounter wet conditions. Examples of pesticidal formulations which contain the pesticide in a hydrophilic matrix include:
(1) The use of hydrated fibrous mats as carriers by Balassa in US patent

(2) The use of thermoplastic hydrogels as carriers by Vaughan et al in Australian Patent AU07680991.
(3) The use of reversibly dehydrated vegetable and/or fruit to provide rodent baits by Barth et al in EP 86107928
(4) The use of a carrier phase comprising milk solids and sucrose (in the presence of high levels of boric acid as active ingredient) by Nelson et al

in US 6,153,181. Nelson points out that toxic baits for crawling insects have historically been water-based, and that water has been presumed necessary for good bait performance. Nelson explains that products result of water loss, rancidity, break-down of active ingredients etc.
(5) The use of an aqueous plant fibre slurry (which is subsequently dried) as the matrix for an agricultural granule has been described by Lowe et al in US 5019564. Lowe et al note that the use of clay in the matrix can create chemical inactivation of active ingredients such as chlorpyriphos.
(6) The use of polyvinyl alcohol and borate in water (subsequently dried) as a pesticide matrix has been described by Maglio in US Patent RE33. 670.
(7) The use of portions of corncob In various ratios as a carrier for pesticides has been described by Katz et al in US 4563344.
The discussion of the background to the invention herein is included to explain the context of the invention. This is not to be taken as an admission that any of the material referred to was published, known or part of the common general knowledge in Australia as at the priority date of any of the claims.
None of the above formulations has been shown to provide long-term ingestible bait which properly contains active ingredient.
Statement of the Invention
The invention provides a granular edible pesticidal composition comprising: a) a continuous hydrophilic matrix phase comprising hydrophilic material, preferably in particulate form and water, said matrix phase being palatable to pests; and b) a discontinuous oleophilic phase dispersed within the hydrophilic matrix phase and comprising an oleophilic carrier and pesticide preferably dissolved in the oleophilic phase.
Summary of the Invention
It is surprising that the discontinuously dispersed oleophilic phase enhances the containment of the oil-soluble pesticide because the principal barrier to release would be expected to be die hydrophilic matrix.

In one preferment the oleophilic phase is viscous at ambient temperature, i.e. the time taken to pour said oleophilic phase from a 100ml beaker is In excess of 10 seconds at 20'^C. and more preferably in excess of 30 seconds at 20'C. The oleophilic phase will preferably have a Brookfield viscosity greater than lOOcP, more preferably greater than 2a0cP. (Measured at a tennperature of 25°C.).
The invention further provides a method of controlling ground dwelling pests in a region comprising applying the granular pesticidal composition as hereinbefore described adjacent or below the surface of the soil.
In regions of thick vegetation the granules may be applied to the thatch of vegetatior^ adjacent the surface of the soil.
In yet a further aspect the invention provides s method of preparing a granular pesticidal composition as hereinbefore described comprising:
(i) mixing water with the hydrophilic phase to form a defonnable
dough; (ii) spraying an oleophilic phase onto the hydrophilic phase and mixing; (iii) forming the mixture into granules; and (iv) drying the granules to mechanical integrity.
Detailed Description of Preferred Embodiments
The pesticidal composition of the invention comprises a discontinuous oleophilic phase which typically contains the pesticidal dissolved therein.
Preferably the oleopt^ilic phase accelerates the rate of water lo-^« from thp matrix phase under the drying time test which will now be described. \r\ the drying time test water is added to the hydrophilic matrix phase to achieve 200 units of matrix phase at 60% moisture (i.e. water comprises 120 units thereoO-10 units of oleophilic material at 70'C are sprayed onto the matrix phase under agitation, and the mixiure is then pelletised (by exirjsion or compaction). If necessary starch powder may he added to the minimum amount required to

ensure that the pellets retain their physical integrity. The pellets are placed in an oven at 70°C and the time taken to dry the granule from 60% to 10% moisture is noted.
Suitable oleophilic materials are those in which the drying time (compared to the case when no oleophilic materia! i& added) is decreased by a factor of 20% or greater.
It Is surprising that the use of an oieophillc material which accelerates the rate of water loss from a water-swollen hydrophilic matrix phase can lead to enhanced containment of the oil-soluble pesticide.
The composition of the invention comprises a continuous hydrophilic matrix phase comprising hydrophilic material and water. The hydrophilic material is typicaily a particulate solid. Preferably these particulate entities comprise eccentric particles with a ratio of maximum dimension to minimum dimension of at least 2, more preferably at least 5. Even more preferably the hydrophilic entities comprise fibres or fibre segments of length 0.05mm or more, most preferably 0.5mm or more.
The continuous hydrophilic phase contains water. The water may be present in amounts of at least 0.6% by weight of the hydrophilic phase and is preferably present in amounts of at least 5% by weight of the hydrophilic phase. The preferred upper limit foi watei will generally be governed by the desired mechanical integrity of the composition. Typically no more than 30% by weight.
In one preferment the hydrophilic matrix phase, when swollen with water, can be formed into a deformable dough ur'^or the action of hi?h prcsswre s'i^;aii;;y forces.
The hydrophilic matrix phase 's edible to target pests. The hydrophilic matrix may comprise a wide range of organic materials aiyiough decomposed plant material and plant fibres is particularly preferred. The hydrophilic matrix may comprise edibie material such as stable composted material, plant fibre, plant

husks, raw or process cereal, blood and bone, bone meal, peat, animal manure and mixtures thereof.
In one particular prefenTie:;; the hydrophilic rnatrix comprises peat or com fibre, preferably wood peat or reed sedge peat or sphagnum peat. More preferably fibrous reed sedge peat Most preferably the hydrophilic matrix comprises a fibrous peat.
In one preferment the hydrophilic matrix phase has a buffer capacity such that the inside of the granule (under prolonged soil storage conditions) can be maintained at 1 or more pH units different from the surrounding soil, preferably 2 or more units.
In one preferment the Inside of the granule has a neutral or acid pH value. This neutral or acid pH is preferably maintained even when the surrounding soil is at pH 8.5 or greater.
The oleophilic phase will generally contain the pesticide as a minor component on a weight bases. The amount of pesticide will thus nom-tally.lje iess.thaa 505:^ by weight of the oleophilic phase. More preferably the amount of pesticide is no mpre than 40% by weight of the oleophilic phase. The amount of pesticide is most preferably from 0.001 to 33% by weight of the oleophilic phase.
In a particularly preferred embodiment of the invention the carrier of the oleophilic phase comprises chlorinated hydrogen, preferably containing at least 8 carbon atoms, more preferably at least 12 carbon atoms and more preferably from 12 to 20 carbon atoms. The degree of chlorinatlon of the wax is preferably 40% c ^roqtftr^ more prefersbly the nhic'irialT?':" VHA is Ceieclor AS52 sow by Orica Australia Pty Ltd of Melbourne Australia, The chlorinated hydrocarbons are in the form of viscous oils or waxes
The granules preferably retain their morphology in soil over a 1-3 year period which includes numerous wet/dry cycles.

Preferably the granules do not kill pests except by Ingestion. In particular it is prefen-ed that the granules do not act as a contact poison, even if the pesticidal agent is a contact poison. Preferably when the granules are in close contact with large inEscts such as cockchafers or whitegrubs they are not injurious.
Preferably the granules do not deposit peslicidal concentrations of pesticide in the region proximal to the granule. If the granules are located proximal to a vertical porous membrane in a sub-soil environment, pests on the other side of the porous membrane are not injured, even if the porous membrane is permeable to the pesticide.
The pesticide w/hich is present in the composition of the invention Is preferably an oil soluble pesticide, more preferably a volatile, oil soluble pesticide and more preferabty an organophosphate such as chlorpyrifos. Preferably at least 50% by weight of the pesticidal agent remains contained within the granule after 3 months of residence in soil, more preferably at least 50% by weight after 6 months and more preferably 80% by weight. The invention Is most suited to using insecticides which have a validity which provides a vapour pressure of at least one miltipascal as measured oy ASTM D5191.
The composition of the invention is particularly suited to control of soil and thatch dwelling pests. These pests include members of the Classes; insects (Class InSGcta) , nematodes (Phylum NematodaJ, mites (Class Arachnida, Sub-Class Acari), spiders (Class Acarina. Order Araneae), slugs and snails (Class Gastropoda), Millipedes (Class Diplopoda), springtails (Cass Collembola). symphylids (Class Symphyla).
Th.9 nrgnijtoc will tvp!,-'.;y bc placed on or ueneai'ti ihe surface or the scii or where thick vegetation is present, they may be p'aced In the thatch covering adjacent the surface of the soil, The procedures which will typically be used for placing the granules may include cultivation of soil on which granules are placed or injecting or drilling the granules into the soil or vegetation thatch.

In one preferment the hydrophilic matrix phase including water makes up 60 -50% more preferably 80 - 95% and most preferably 80 - 90% by weight of the total composition. The oleophilic phase typically comprises 1 - 25% by weight, preferably 5 - 25% by weight (based on the weight or ihe oleophilic phase) of pesticide, such as chlorpyrlfos, preferably in chlorinated wax. The whole oleophilic phase preferably mates up 5 - 40% more preferably 5 - 30% and most preferably 5 - 20% by weight of the total weight of the composition.
In the case of peat the hydrophilic matrix phase is typically dried to provide a water content in the finished product of up to 30% wAv and more preferably 5-25% w/w. In one specific example the pesticidal composition contains 9% w/w Cerachlor, 1% chlorpyrlfos w/w, 20% w/w water and about 70% w/w peat (on dry weight basis).
We have successfully made baits using an oil phase as high as 20% w/w of the total final product.
There is disclosed a method for mal (1) adding water to the hydrophilic phase until the phase can be fornied into a deformable dough under pressure;
(2) spraying oleophilic phase onto the hydrophilic phase and mixing, for example in a rotary drum;
(3) forming the mixture into granules for example by a method selected from extrusion, compaction granulation, basket granulation or other methods known to the art; and
(4) drying the granules.
Preferably the extruder achieves a compaction ratio of at (east 1.5, preferably at least 2. Preferably a single screw front plate exlruder is used to form the granules.
Preferably inner and outer cutter blades are placed proximally to the extruder die plate. The inner cutlet blades cut fibres which bridge between orifices in the

front plate. The outer cutter blades cut the extruded rods Into granules. We have found that the use of internal cutting blades is vety important with peat and compost and other long fibred rnateria! but less so with material such as blood and bone. With peat and fibrous materials the outer cutting blades are less necessary than the inner cutting blades. The outer cutters are used to get the bait granules to the desired length hut this can be achieved, through other means such as in a rolling drum.

Treferablyjhe granulesare. dried w^the-grarTqtera£hieve'ari indivtdUall crush
- --- ——-"^ - -
strength of at le^sl 500g and more preferably at leasHOOOg.
The simultaneous use of both inner and outer moving cutter blades proximalty to the extruder die piate is believed to be a novel feature of the pelletisation process.
Throughout the description and ciaims of this specification, the word "comprise" and variations of the word such as "comprising" and "comprises", is not intended to exclude other additives or components or integers,
The Invention wit) now be described with reference to the following examples. It is to be understood that the examples are provided by way of illustration of the invention and that they are in no way limiting to ^e scope of the invention.
Examples
Example 1: Formulation of inseciicidaJ chhrpyrifos baits Fibrous reed sedge peat (60% moisture) was put thiough a thresher to provide size comminution, leaving the peat as a loose particulate mass comprising fibres aenerally less than 5mm Innn
The peat was taken from the Peat Operations mine on Tinengower property, Swan Marsh Irrewillipe road, Swan Marsh District. Colac, Victoria Australia. The fibrous peat generally is located in the upper 600mmm of the resource.

1.2g of chlorpyrifos was heated to 50*C and dissolved in 9g of chlorinated paraffin wax at 50°C. The chlorinated wax was a C14 wax and was 52% chlorinated. This material is sold undef the trade name Cereclor AS52 by Onca Australia Pty Ltd of Melbourne Australia.
The oleophilic wax phase (10g) was sprayed onto 197g of fibrous reed sedge pest phase (60% moisture) under agitation by a rotary stirrer. The dough was extruded through a Moulimex single screw front plate extruder to form granules of dimension 3mm dtameter x 6mm length. The granules were dried in a fan-forced oven at 70°C for 5 hours. The final granules were measured to contain 1% chlorpyrifos and 20% water.
Example 2: Chlorinated wax accelerates the rate of water loss from fibrous
reed sedge peat granules at 60% moisture. ExtnJded granules were made according to the process of example 1 but without the drying step and without the use of chlorpyrifos. Granules designated G1 were made without the addition of chlorinated wax, and granules designated G2 were made with the addition of 9 grams chlorinated wax. 5g samples of granules were placed onto 10cm diameter aluminum foil dishes and placed in a fan forced oven at 70°C. The dishes were weighed at regular intervals after being placed iri the oven.
The time taken for G1 granules to reach 10% water content was 125 minutes, and the time taken for G2 granules to reach 10% water content was 40 minutes.
Example 3: Peat Granules are edible to greybaci (Denvoiepida albohirtum) Eggs taid by adult beptles were colSe.cterf arid the f!rs» instar g.-bc v;cr^ s'-'ovv-w to hatch. Each of 15 replicates consisted of a single grub which was placed in moist sand in a 70ml vial. The newly hatched cane grubs burrowed into the sand, and then a black reed sedge peat pellet (containing no oleophilic inclusions or chlorpyrifos) was placed on top of the sand surface. The trial continued for 7 days, Examination of peat pellets showed eroded segments with bite marks which could only be explained by larval feeding. Examination of

grUDs (the gut .regions were transparent) showed black peat fragments in their gut.
Example 4: 1% cNorpyrifos peat bait made accoraing to the inwniion cfves not
kill wf^ite j/rujbs by contact activity 1% chlorpyrifos granules were made according to tfie method of example 1, When ingested, these granules provided 100% mortality of Z"^ instar cockchafer larvae, however when the granules were adhered to the front of 3"* instar lar^'ae using surgical adhesive tape, the mortality was not significantly different from controls.
Example 5: 1% chlorpyrifos baits made according to ttiis invention properly
contain the chlorpyrifos within the bait 1% chlorpyrifos granules were made according to the process of example 1. Granules designated G2 were made according to the Invention using an oleophiiic phase comprising 1 part chlorpyrifos dissolved in 9 parts chlorinated wax. Granules designated G3 were made by adding neat chlorpyrifos to the hydrophilic phase (no additional oleophilic material was included). Granules wore buried 1 cm into aiicaline Wimmera clay (pH 8.5) from the Wimmera region of Victoria Australia, which had been remoistened with water (to 25% water) and placed Into 500ml tins with approximately 2cm of air space at the top. The tins were sealed and placed into an oven at 35°C.

After 3 months incubation at 35*^0, granules designated G2 were found to contain 85% of the chlorpyrifos originally present. Granules designate G3 were found to contain only 20% of the chlorpyrifos originally present
Example 6; This example demonstrates the control of a range of ground dwelling pests using components of the invention.
Example, 6a: Chlorpyrifos baits made according to this invention were us&d to
control cane grub larvae in laboratory tests First instar cane grub larvae {Dermolepida albohirtum) were used as test individuals. The test was conducted at 25°C. Peat granules were made according to the method of example 1, but with varying amounts of chlorpyrifos. in one treatment the reed sedge peat was replaced with humic peat, a peal from the same mine but which contains far less organic matter. (See Table 2).

soma treatments (see Table 2), and was placed below the bait granule so that the (arvae would have to pass the attemative food source to find the bait.
At 5 days after treatment (DAT) there were no dead gaibs in the control, however at least 80% of goibs had died In all treatments containing granules with chlorpyrtfos concentrations of 0.05% or more. Of the treatments containing 1% chlorpyrtfos, all had 100% grub mortality.
By 7 DAT, all chlorpyrifos granules had led to 100% grub mortality, although no grubs had died in the control.
Example 6b: Chlorpyrifos baits made according to this invention were used to control white grub larvae (Acrossidus tasmaniae) in laboratory tests Two concentrations of chlorpyrifos, 0.1% and 1%, were used to prepare granule samples as per Example 1 and another sample of granules was made omitting the chlorpyrifos and is designated as Control + Peat. The protocol for evaluation of bioefficacy on gnjbs was the same as for Dermoiepida a}bohirtum in Example 6a,
15% of grubs in the Control and 25% in the Control + Peat treatment died during the experiment. By comparison 100% of gnjbs were dead within 2d for the 1% granules and within 4d for the 0.1% granules (see Table 3).

Examph 6c; Chhrpyrifos baits made according to this invention were used to control an alternative canegnjb species (Lepidiota negatona) in iaboratory tests Granules were prepared as per Example 6b. Or.^ sample of granules was made omitting the chlorpyrifos and is designated as Control + Peat, The protocol for the evaluation of tjloefficacy on grubs was the same as for Dermolepida albotiirtum in Example 6a,

Mortality of crickets for the treatment receiving the bait was 100% while in the untreated controls it was 0%.
Exttmple 7. ManufactUie of a batch of poiticida! granules
Threshed reed sedge peat (19.7kg, 60% moisture content) was placed in a
rotating drum cement mixer (0.8m max diameter tapered drum leading to 0.4m
orifice).
120g of chlorpyrifos was dissolved in 900g Cereclor AS52 chlorinated wax at 50°C under agitation. This oleophilic phase was drip fed into the reed sedge peat under agitation in the cement mixer over a 5 minute period, and the mixer was kept running for a further 5 minute period.
The contents of the cement mixer were processed in a Fabio Leonard! 0.7 HP front plate extruder. (Fabio Leonard! are based In Bo, Italy).
The front plate was 8cm in diameter and the holes were 3mm in diameter. The extruder used a variable-pitch single screw providing a compression ratio of 2:1. The extruder was purchased with a fitted internal cutter mounted to the screw.
An externa! cutter comprising a sharp steel blade was joined to an electric drill bit and was rotated independently of the extruder screw. The cutter was located on the external face of the die and was used to chop the extruded strands into granules. The extemal cutter was rotated in a reverse sense to the rotation of the extruder screw.
The resultant granules (3nrim diameter x 5mm length) were dried in a drum drier Im .-"- r.r;ri ZOc-r. Jistp, and ihs exterior or tne steel drum was directly heated by a gas flame.
The granules were dried to 15% moisture and were measured to contain 1% chlorpyrifos by weight

gxampie 8: Failure of conventional methods to produce robust fibrous reed
sedge peat granules Fibrous reed sedge peat (60% moisture) was added to a Fuji Paudat model EXDTF100 extruder with a 3mm die. The trial extrusion took place at the laboratories of Fuji Paudal in Osaka, Japan on Sept 11 & 12. 2000.
After 36 seconds of operation, the die became blocked as the peat fibres were laid flat against the internal face of the die by the motion of the screw.
Fibrous reed sedge peat was added to a range of commercial pellet mills which utidsed a rotating kneading action to force the peat through s die. All these pellatisers became blocked within 10 minutes of operation. Furthermore the granules (before blockage occun-ed) were not homogenous and compact but were striated In morphology (reflecting the action of multiple pressure pulses in the pelletiser). The granules thus produced snapped readily and were not robust enough for application to and stable residence in soil.
Example 9: Field Trial - Control of cane grub
Sugar cane was planted at hvc sites in July 2000. IK Octobei 2000, 10m x 6m plots comprising four planted rows were laid out and 0.5% chlorpyrtfos granules made according to the method of Example 7 but with half quantities of chlorpyrifos and chlorinated wax were broadcast by hand at a rate equivalent to 250kg per hectare. The granules were incorporated to a depth of I5cm using a power harrow. Flights of adult greyback cane beetles {Derrnolepida albottirtum) took place in December 2000 - January 2001, and in February four cane stools per plot were dug up from the two middle rows and the number of grubs per stool was counted. Each treatment was replicated 5 times, i.e. a total of 20 stools were dug up for each treatment. Ths rf>Rriit« vA/(?re e.>:p."accc:d in t"rm= o' the average number of grubs per stool (see Table 5). At a third site a similar experiment was lard out in a sugar cane ratoon (re-growth from cane cut in previous season).
Trials were carried out at site 1 (Farmer Romeo, Burdekin District, Queensland, Australia), site 2 (ratoon crop, Farmer Sgaibossa, Burdekin Districl.

Queensland, Australia) and site 3 (Farmer Marciilio. Tully District, Queensland, Australia).

Sxampie 12: 3mm b&its made according to the invention from a number of
Insecticide ct^smical groups are storage stable A sample of 3 mm diar. igter baft granutes wa? made from each of the active ingredients according to Example 1 except that chlorpyrifos was replaced with one of the active ingredients in the table below and for imidachfoprid 0.12 g of imidachloprid was substituted for 1.2g of chlorpyrifos. lOOg samples of each of these granules were taken arid divided into four. 25g duplicate samples were placed into a 50ml, sealed glass vials and placed in an oven to be kept at 54'C for 14d. The other two duplicate samples were analysed for active ingredient The samples taken from the oven after I4d were also then analysed for the active Ingredient content.
This test not only evaluates the storage stability of the granules but also estimates the relative loss of active ingredient when in the soil for a prolonged period. When an oleophilic phase was present all active ingredients were found to be stable during 14d of storage at 54°C with none degrading by more than 8% (see Table 7),

evaluation of bioefficacy on grubs was the same as for Example 6b except that mortality was measured on second - third instar grubs rather than new hatched grubs and at 3d after treatment.

Example 14: Baits made from other actives according to this invention control
termites (Coptotermes acinaciformis) in laboratory tests Samples of granules were prepared as per Example 13 using bifenthrin, carbaryl. diazinon, methidithion and trichlofon plus a sample was made up containing 1% chlorpyrifos granules . "Xhe protocol for the evaluation of bioefficacy on termites was the same as for Example 13 except that 5 Teplicates each of 5 worker caste termite individual were used.
No termites in the Control died during the experiment. By comparison the mcriclitj in tr;,ct,*.fius contalnlriy in^BuilcIdes ranged from TO -yb'fo {zee Tsbie 9).

Example 16: Chlorinated wax reduced chlorpyrifos losses from a wide range of
edible, hydrophjiic matrices The hydrophiiic matrix miist br edihle tc soil borne pests and campatible '/.-iih the oleophilic phase and active ingredient and retain the active ingredient during the drying stage of manufacture and iater when in the soil for prolonged periods. An elevated temperature tests can be used to estimate the relative losses under these conditions and so the relative suitability of these matrices,
Water was sprayed onto 500g of each matrix (see Table 12), while continually stirring, until a small amount could be extruded successfully through a Moulimex single screw front plate extruder to form granules 3mm in diameter to form the "pre-wet matrix". The chicken manure matrix was made by breaking up Yates' Dynamic Lifter chicken manuie pellets using a mortar and pestle prior io adding the water. The moisture content of each "pre-wet" matrix was then determined. One part chlorpyrifos was then added to 9 pari:s Cerachlor AS52 to form the "liquid blend A" or alternatively one part chlorpyrifos was then added to 9 parts acetone to form "liquid blend B". The moisture content of the matrix was then used to calculate the amount of either "liquid blend A or B" that was required to be added to the "pre-wet matrix" to result in approximately 1% chlorpyrifos after

granules weie dried for 3h at 7Q°C. The relevant estimated amount of "liquid blend" was then added to the "pre-wet matrix". This sample was then passed through the extruder to form granules. These granules were spread thinly onto a stainless steel tray and placed into a fan forced oven at 54'C for 2 weeks. The granules were then removed and their chlo[pyrifos content determined.

Example 17: Oleophilic phase reduces chlorpyrifos losses under a temperature
challenge The oleophilic phase must be able to act as a scivent (or at Inast be miscible) with the active ingredient and reduce the loss of the active ingredient during the drying stage of manufacture and later when in the soil for prolong periods. An elevated temperature tests can be used to estimate the relative losses under these conditions and so the relative suitability of the oleophilic phase candidates. Under this test suitable candidates will reduce losses to one third of that of active ingredient alone.
1.2g of chlorpyrifos was added to 9g of each alternative oleophilic candidate (see Table 14). 1,02g of this liquid blend was then placed onto aluminium foil within a glass Petrie dish. The control was molten chlorpyrifos alone. Samples were then placed in a fan forced oven at TCC. for 70h before being removed and analysed for chlorpyrifos content.

Example IS: Alteniativv nieophilic phases reduce chlorpyrifos losses undei a
tamperature challenge 1% chlorpyrifos granules were made according to the process of Example 1 with the exception that the Cerachlor AS52 was substituted for 5 of the other candidates in Table 14. Each granule sample was divided into two sub-samples and one was placed into a fan forced oven at 54C for 14d and the other was analysed for chlorpyrifos content When the sub-samples were taken from the oven they too were analysed for chlorpyrifos.
None of these candidates performed particularly well in this test hut chnrter chain chlorinated paraffins with greater chlorination make the best oleophilic phase for chlorpyrifos (see Table 16).

Example 19: 1mm diameter and 7mm diameter baits made according to the Invention using the organophosphaie chloipyrffos are storage stabiB Four samples of granules were made. One sample of granules were made according to Example 1. the second sample was made using the same method except the granules had a diameter of 7mm. The third and fourth sample were made according to Example 1 except that >n_«iune was substituted for the oleophilic phase. Sample 3 had a diameter of 3mm and Sample 4 had a diameter of 7mm.
lOOg sub-samples of each of these granules were taken and divided into Tour. Duplicate 25g samples were placed into a 50ml, sealed glass vial and placed in an oven to be kept at 54'*C for 14d. The other duplicate samples were analysed for active ingredient. The samples taken from the oven after 14d were also analysed for the active ingredient content.
In the absence of the oleophilic phase 45% of the chlorpyrifos was lost from 7mm granules during 140 of storage at 54°C compared with 1"!% when the oleophilic phase was present (see Table 17).

The hydrophilic matrix must be edible to target pests.
Granules were made up from 11 hydrophilic matrices (see Table 19) as per Example 16, with the sxceptiL., th:it ='! i_.,"r-:jl£S v;er£ made up vvit!'- OT;=:L;!:i'jr AS52 and none with acetone.
Bioassays Were undertaken on white grubs (Acrossidus tasmaniae) as per Example 6b and on termites (Copteterwes acinaciformis) as per Example 14-

Example 21: 1% chhrpyhfos baits made according to this invention properly contain chlorpynfos witliin baits made from a range of edible, hydrophilic matrices The edible hydrophilic matrix must be able to rciain the active- ingrGrtient not just during the drying stage of manufacture but also later when in the soil for prolonged periods. An accelerated loss test can be used to estimate the relative losses in soil.

©ranulQS were made as for Example 5 except that the hydrophilic matrix was Austrslian iignin peat Irom Eco-Gro International, Wlallanda, Queensland, Australia. These granules are designated as G3. Whereas reed sedge peat has been formed by the degradation of reed sedge, Iignin peat has resulted from the degradation of wood and trees, and djffeis in composition. Lignin peat is also called wood peat or woody peat. This data has been tabled against the data from Example 5.

After 6 months of storage at 35°C there was littte difference in the rate of chlorpyrifos losses from G2 or G3 granules from these two peats that have very different origins and compositions (see Table 24).

Examp/g 23. B&Hs made Bccording to this invention properfy contained a number of aitemative active ingredients when placed into the soil af elevated temperature tt has been shown previously that the Invention can oroperly r^^tain chiorpyrifbs in the hydrophilic matrix when stored in soil. In this example other active Ingredients are also shown to be held within a reed sedga peat matrix.
A 3mm diameter bait was made as pre Example 1 except that the chlorpyrifos was replaced with bifenthrin (synthetic pyrethroid), carbaryl (carbamate), methidathion (organophosphate) and endosulfan (organchlorine).
The granules were placed into soil at elevated temperature as per Example 5 for one month.
After one month in the soil more than 50% of the originai active ingredient was found to remain in the granules. This would be far more than that required to control pests (see Table 25).

Example 24: Granules made at two ratios of active ingredient to ail phase Qccofdfng to (his invention property contained the active ingredient when placed into the soil at elevated temperature tt has been shown previously that the invention can properly retain chlorpyrifos in the hydrophilic matrix when made in a ratio of Iparl chlorpyrifos to 9 parts oil phase. In this experiment the ratio of 1 part chlorpyrifos : 3 parts oil phase was used in a 5% chlorpyrifos granules and 1 part chlorpyrifos: 99 parts oil phase in a D.1% granules.
Granules were niade as pre Exampie 1 except that either Sg.of chlorpyrifos was added to ISg of Cerachlor AS52 and sufficient added to make 5% chlorpyrifos granules or O.lg chlorpyrifos was added to 9g Cerachlor AS52 and sufficient added to make 0.1% chlorpyrifos granules. The granules were placed into soil at elevated temperature as per Example 5 for one month.
After one month in the soil more ttian 93% of the original chlorpyrifos was found to remain in the 5% granules while 96% was found in the O.T % granules.
Example 25:_ The solubility of (he active ingredient in the olf^nnhiHc phzz^
effects the performance of (his phase. It was found that the preferred oleophilic phases provided a solubility for the pesticide of at least 20% w/w, more preferably at least 30% w/w and still more preferably at least 40% by weight based on the combined weight of oleophilic phase and pesticide.

The solubility of chlorpyrlfos was determined for the oleophilic phase candidates in Example 17.
It was found that preferred candidates could contain 20% w/w of chlorpyrlfos in the weight of chlorpyrrfos plus oleophilic phase and preferably 30% and more preferably 40%.
Example 26: 1mm diameter baits made according to the invention using
alternative pesticides ere storage stable 1mm diameter samples were made and treated according to Example 19a except that the chlorpryifos was replaced with the alternative pesticides, diazinon or terbufos.
In the absence of the oleophilic phase a large proportion of these volatile pesticides were lost during bolh the drying phase and the storage phase.
In the absence of the oleophilic phase approximately 30% of terbufos was lost during drying and then, based on the terbufos content of the dried granules, P further 70% was lost during storage rn soil. In the p^essnce of the oleopniiic phase this reduced to approximately 18% and 48% respectively (see Table 26).
In the absence of the oleophilic phase approximately 6% of diazinon was lost during drying and Ihen, based on the diazinon content of the dried granules another 64% was lost during 14d storage in soil. In the presence of the oleophillic phase this reduced to approximstely 0% and 49% respectively.

We Claim:
1. A granular odibic pGSticidal composition comprising:
(a) a hydrophilic matrix comprising a hydrophillc material and vvak'r, said matrix being palatable to pests; and
(b) a discontinuous oleophilic phase dispersed within the hvdrophlic matrix and comprising an oleophilic carrier and a pesticide;
wherein said composition comprising 70 to 95% by weight of the h\ drophilic matrix and 5 to 30% by weight of the oleophilic phase and ha\'inj', an individual crush strength of atleast 500g.
2. 'I'hc granular pcsticidal composition as claimed in claim 1 wherein, following storage at 35°C for 12 months in alkaline Wimmera clay {pi i 8,5) which has been rcmoistcned with water (to 28%), the composition retains at least 70% ol the quantity of the pesticide present in the composition prior to said storage.
3. The granular pesticidal composihon as claimed in claim 1 or claim 2, wherein the hydrophilic matrix in the composition prior to said target pests.
4. The granular pcsticidal composition as claimed in any one of claims I to 3, wherein the hydrophilic material is in particulate form.
5. The granular pcsticidal composition as claimed in any one of claims 1 to -1, wherein the pesticide is dissolycd in the oleophilic phase.
6. The granular pcsticidal composition as claimed in any one of claims 1 t(^ 5,
wherein the amount of pesticide is less than 50% by weight of the oleophilic
phase.

7. The granular pesticidal composition as claimed in any one of claims 1 to 6, wherein the discontinuous oleophilic phase enhances the containment o( the pesticide.
8. The granular pesticidal composition as claimed in anv one of claims 1 lo 7 comprising from 0.001 to 33% by weight, based on the weight of the oleophilic phase, of the pesticide.
9. t'he granular pesticidal composition as claimed in any one of claims 1 to S comprising from 5 to 20% by weight of the total composition o( the oleophilic phase and HO to 95% bv weight of the total composition of the hydropiiiiic matrix.
10. The granular pesticidal composition as claimed in any one of claims 1 lo 9, wherein the oleophilic phase has a Brookfield viscosity at a temperature of 25" C greater than lOOcP.
11. The granular pesticidal composition as claimed in claim 10, when^in the oleophilic phase comprises one or more oleophilic carriers selected from the group consisting of chlorinated hydrocarbons and polyalkylene glycols,
12. The granular pesticidal composition as claimed in any one of claim 10 or claim 11, wherein the oleophilic phase comprises a chlorinated hydrocarbon comprising atleast 12 carbon atoms and having a degree of chlorination of atleast 40%.
13. The granular pesticidal composition as claimed in any one of claims 1 lo 12, wherein the hydrophilic material is selected from the group consisting of

processed or unprocessed cereal grains, blood and bone, peal ami animal manure,
14. The granular pesticida! composition as claimed in any one of claims 1 io 13, wherein the hydrophiUc material is peat.
15. The granular pesticidal composition as claimed in any one of claims 1 to 14, wherein the pesticide has a vapour pressureof atleast one milipascal,
16. I'hc granular pesticide as claimed in any one of claims 1 to 15, wherein the pesticide is selected from the group consisting of organophosphale, insecticides, organochlorinc insecticides, carbamate insecticides, synthetic pvrethroids, guanidinc/neonicotinoids and mixtures thereof.
17. Ihe granular pesticidal composition as claimed in claim 16, wherein the pesticide is an organophosphale.
18. 'J'he granular pesticida! composition as claimed in any one o( claims 1 to 17, wherein the oleophilic phase provides a drying time (compared to tfie case when no oleophilic material is added) which is decreased bv a factor of 2(1% or greater.
19. The granular pesticidal composition as claimed in any one oi claims 1 to 18, wherein granules of the composition have a maximum dimension in the range of from 0.5 to 10mm.
20- The granular pesticidal composition as claimed in any one of claims 1 to 19, wherein granules of the composition are extruded.

21. The granular pesticidal composition as claimed in any one of claims 1 to 20,
wherein the hydrophilic matrix has a buffer capacity under prolonj'ed sLoraj',e
conditions.
22. Ihe granular pesticidal composition as claimed in anv one of claims 1 to 21, wherein the granules maintain their morphology in soil over a 1-3 vear period,
23. 'i'he granular pesticidal composition as claimed any one of claim.s 1 io 22, wherein tlie granules do not act as a contact poison.

Documents:

1015-chenp-2004 abstract duplicate.pdf

1015-chenp-2004 abstract.pdf

1015-chenp-2004 claims duplicate.pdf

1015-chenp-2004 claims.pdf

1015-chenp-2004 correspondence others.pdf

1015-chenp-2004 correspondence po.pdf

1015-chenp-2004 description (complete) duplicate.pdf

1015-chenp-2004 description (complete).pdf

1015-chenp-2004 form-1.pdf

1015-chenp-2004 form-13.pdf

1015-chenp-2004 form-19.pdf

1015-chenp-2004 form-26.pdf

1015-chenp-2004 form-3.pdf

1015-chenp-2004 form-5.pdf

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Patent Number

227965

Indian Patent Application Number

1015/CHENP/2004

PG Journal Number

10/2009

Publication Date

06-Mar-2009

Grant Date

27-Jan-2009

Date of Filing

12-May-2004

Name of Patentee

GROTECH AUSTRALIA PTY LTD

Applicant Address

4 DAIRY ROAD, WERRIBEE, VICTORIA 3030,

Inventors:

#	Inventor's Name	Inventor's Address
1	FLYNN, ANTHONY, GERARD	24 JAMES COOK DRIVE, WANDANA HEIGHTS, VICTORIA 3216,
2	PENTLAND, PHILIP, EDWARD	520 RACECOURSE ROAD, FLEMINGTON, VICTORIA 3031,
3	FAN, HONG, SHU	9 GREENLOW AVENUE, WANTIRNA, VICTORIA 3152,

PCT International Classification Number

A01N25/12

PCT International Application Number

PCT/AU02/01110

PCT International Filing date

2002-08-16

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	PR 7099	2001-08-17	Australia