|Title of Invention||
"A MOSQUITO LARVICIDAL PREPARATION OF BACILLUS THURINGIENSIS VAR. ISRAELENSIS"
|Abstract||This invention relates to a mosquito larvicidal preparation comprising a cell mass obtained from a seed of bacillus thuringiensis var israelensis bacterial strain in sodium alginate and water aid extruded as granules into calcium chloride solution.|
|Full Text||This invention relates to a mosquito larvicidai preparation of bacillus- thuri noi ensvi. s. var. israel ensis.
Baci i i us thur i noi en si s var . i srael erisi sis » known bacterial strain and processes for the prepai'"fation t hereof «
a oiosqui to larvicidal preparation o-f bacillus
thur i noi ensi y. visr . i sraerl e-nsj B havina e-ui tebl e le-vet of .(. arvicida.l activi tv.
A further object of this invention is to propose a mosquito larvicidal preparation -for preparina a SE} ow—rel e«»e floatinq formulation of bacillus thur i nai ensi s var. i srael lansis to serve as a more efficient agent for des-trovinct mosquito larvae,
At the outset of the description follows. it i 5- to be understood that the- ensuing description only .illustrates a particular form af this invention. However, such « particular form is only an exemplary
embodiment. and without intend!nq to implv any limitation on the scope o-f this invention. Accordinoly. the deavcr i pti on is to be understood «s an exemplar v embodiment and teach!no of the invention and not intended to be taken restrict!velv.
According to this invention, there i« provided a mosquito larvicidal preparation compri si no a cell
subjected ta the step of fermentation in a nutrient medium containing s-oyaflour, peanut oil and sterile water at a pH of 7.
step of mixina ceii mass with sodium aloinat.e. said
mixtv>re JK diluted with vjater. extruded && aranules^ into
calcium chloride solution and »aid qranulcs allowed to
cure. She granules are then dried and packed ir«
perforated sachets with polystyrene heads.
A mos-qu i to larvicidel preparation of bacillus
thur.i ngiensis var. :i. sraelensi » acc.ordinci to a preferred
embodiment is herein described «nd illustrated in the
accompanyino drawings wherein:-
Ficj.l shows the flow chart of the process.
The process proposed by t.he present invention and as shown in the flow of chart of fiq.1 comprises transferrino the seed/spores of the strain, obtained from the lyophilized seed lot, into a test tube
the qrown culture so obtained is transferred into a conical -flask havinci 80--120mi preferably 100ml of seed medium as herein described above and sterile water at s pH 7. Aqain the grown culture so obtained is transferred into another conical flask having 1800-2200ml preferably 2000ml of seed medium as mentioned hereinabove and sterile water at a pH 7-7.5 so aa to all the orowth of the culture at a temperature 28-32°C -for 6-9 hr. at 225-275 r.p.m. stirina/ahaking speed.
The qrown culture so obtained is then transferred into a iomenter/bioreactor having 55-65 litre of production medium containing, 2.5% soya-flour or 1^5"/. Jaggery, 0.5V. yea»t extract, 0.01% potassium dihydrogen phosphate, 0.05X magnesium s-ulfate, 0.3X sodium chloride The growth up cellmase so obtained is then harvested from the above culture either by continuous cen^ri fugation at 2S000-3(30
solution and is subjected to the step of curino for 3-6 hr. Vhe cured granules are dried at a temperature 30-40°C for 24-29 hr and the dried granules »o obtained are then packed in perforated aacheta alongwith polystyrene beads at the ratio of 100:1.
1. A mosquito iarvicidal preparation comprieinq A
cell mass obtained from a seed oi bacillus thuri nolens is
var . i E-rael e-nsi s bacterial strain in sodium alqinate and
water and extruded aa granules into calcium chloride
sol uti on,
2. A mosquito Iarvicidal preparation as claimed in
claim 1 wherein sodium alginate comprises a 3 ho 8/.
solution &f s»odiu(Ti alqinate in water.
3. A mosquito Iarvicidal preparation substantially as
herein described and illustrated,
|Indian Patent Application Number||650/DEL/2003|
|PG Journal Number||34/2008|
|Date of Filing||30-Apr-2003|
|Name of Patentee||NATIONAL COUNCIL OF MEDICAL RESEARCH|
|Applicant Address||ANSARI ROAD, NEW DELHI-110 029, INDIA.|
|PCT International Classification Number||C12N 1/20|
|PCT International Application Number||N/A|
|PCT International Filing date|