Title of Invention

"NOVEL 1, 2, 4- TRIAZOLE COMPOUNDS"

Abstract A novel 1,2,4-triazole compound represented by the following general formula (1): (1) wherein R2 represents unsubstituted pyridyl or pyridyl substituted by cyano, etc.; R1 represents unsubstituted pyridyl, pyridyl substituted by halogeno, etc., or phenyl substituted by cyano, etc.; and R3 represents hydrogen or pivaloyloxy-substituted lower alkyl which each is bonded to one nitrogen atom as a member of the 1,2,4-triazole ring in the general formula (1). It is useful as a therapeutic agent for hyperuricemia and gout caused by hyperuricemia.
Full Text NOVEL 1,2,4-TRIA2GLI CGMPQUHD5
BACKGROUND OF THE INVENTION Field of the Invention
The present invention relates to a novel 1,2,4-triazole compound which may be substituted at 1, 2 or 4 position with a substituted alkyl group and has aromatic rings at 3 and 5 positions, a hydrate or a salt thereof, a process for production thereof, and a therapeutic agent for gout and hyperuricemia comprising one of these substances as an active ingredient.
Description of the Related Art
The number of patients with hyperuricemia in Japan is reported to be 1.25 million and that suffering from asymptomatic hyperuricemia is estimated to reach several millions. Hyperuricemia is becoming a popular disease.
Presently, hyperuricemia and gout due to hyperuricemia are treated by improving living environment and administering various drug therapies for each period when attack of gout is predicted to occur (presymptomatic period), when attack of gout occurs, or when attack of gout subsides. That is, preventive therapy is conducted in the presymptomatic period by administering colchicines as well as controlling daily living environment. When an attack occurs, drug therapy using non-steroidal or steroidal antiinflammatory agents is mainly conducted. After the attack subsides, patients are given

guidance to improve their lifestyle. When improvement is judged insufficient, assessment as to whether hyper uricemia is caused by reduced excretion of uric acid or by increased production of uric acid is made followed by drug therapies with drugs, which exhibit a uricosuric effect, such as probenecid and benzbromarone, those which inhibit resorption of uric acid, such as sulfinpyrazone, those which improve acidurea conditions, such as citrates, xanthine oxidase inhibitors which inhibit production of uric acid, such as allopurinol. Colchicine is said to be able to prevent about 90% of attacks through inhibiting chemotaxis and phagocytosis of leukocytes such as neutrophil if administration thereof has been completed within a few hours before the attack. Since colchicine has various adverse effects, however, the use thereof is limited to the minimum and it is therefore difficult to timely administer it.
Accordingly, drug therapies are mainly adopted, but only allopurinol is available for the treatment of disease caused by increased production of uric acid, and a metabolite of allopurinol, oxypurinol, tends to be accumulated and may cause calculus formation. Furthermore, this drug has been reported to induce adverse events such as rash, a decreased renal function and hepatitis, and is not easy to administer.
Examples of compounds having xanthine oxidase inhibiting activity that can be used for treating gout caused by increased production of uric acid and are effective for hyperuricemia and gout due to hyperuricemia have been described in J.

Medicinal Chemistry, 1975, Vol. 18, No. 9, pp. 895-900, Japanese Patent Publication No. 49-46622 and Japanese Patent Publication No. 50-24315, which disclose some 1,3,5-substituted or 3,5-substituted 1,2,4-triazole compounds.
SUMMARY OF THE INVENTION
Hyperuricemia and gout caused by hyperuricemia tend to spread even among young people in Japan due to changes in lifestyle and the like and the diseases cannot be dealt with only by guidance aiming at improvement of lifestyle. However, compounds having improved efficacy for these diseases are difficult to obtain and development of novel therapeutic agents has made little progress by now.
An object of the present invention is to provide a novel 1,2,4-triazole compound which has a high xanthine oxidase inhibiting activity and is useful as a therapeutic agent for hyperuricemia and gout due to hyperuricemia caused by increased production of uric acid.
In short, the first invention of the present invention relates to a 1,2,4-triazole compound represented by the following general formula (1): [Chemical 2]


which may be substituted at 1, 2 or 4 position with a substituted alkyl group and has substituted aromatic rings at 3 and 5 positions, a hydrate or a salt thereof.
The second invention of the present invention relates to a process for production of a 1,2,4-triazole compound represented by the above described general formula {1) in which R3 is hydrogen, characterized in that the process comprises the step of reacting an iminoether of a corresponding aromatic nitrile with a hydrazide of an aromatic carboxylic acid.
The third invention of the present invention relates to a process for production of a 1,2,4-triazole compound represented by the above described general formula (1) in which R3 is a lower alkyl group substituted with a pivaloyloxy group, characterized in that the process comprises the step of reacting a compound represented by the general formula (-1) in which R3 is hydrogen with a halogenated lower alkyl ester of pivalic acid.
The fourth invention of the present invention relates to a therapeutic agent comprising a compound represented by the above described general formula (1) or a hydrate or a salt thereof as an active ingredient.
Under the circumstances that only one xanthine oxidase inhibitor is commercially available, the present inventors aimed at creating a compound having inhibitory activity against xanthine oxidase on hyperuricemia and gout due to hyperuricemia which is caused by increased production of uric acid, and

continued researches focusing on 1,2,4-triazole compounds as a basic substance.
As a result, the present inventors have completed each of the above mentioned inventions.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be specifically described.
First of all, the groups in the above described general formula (1) will be described.
In the above described general formula, R2 represents an unsubstituted pyridyl group or a substituted pyridyl group having a cyano, lower alkyl, halogen, lower alkoxy or lower alkylthio group as a substituent. Ri represents an unsubstituted or substituted pyridyl group which may be substituted with a halogen, cyano or phenyl group, a pyridine-N-oxide group corresponding to these pyridyl groups, a phenyl group substituted with a cyano or nitro group or a phenyl group substituted with, in addition to a cyano or nitro group, a substituted or unsubstituted lower alkoxy group, an N-lower alkyl-piperazino group, a lower alkylthio group, a phenylthio group, or a lower alkylamino group/ provided that Ri is not an unsubstituted pyridyl group, a pyridyl group substituted with a lower alkyl group, or a pyridine-N-oxide group corresponding to these pyridyl groups in the case that R2 is an unsubstituted pyridyl group or a pyridyl group substituted with a lower alkyl group. R3 represents hydrogen

or a lower alkyl group substituted with pivaloyloxy group and in each case R3 bonds to one of the nitrogen atoms in the 1,2,4-triazole ring represented by the general formula (1).
In the case that both the substituents at 3 and 5 positions of the compound of the present invention comprise pyndine rings, at least one of them are preferably substituted with a cyano group or halogen, and in the case that one of the substituents at 3 and 5 positions is a phenyl group, the phenyl group may be substituted with a substituted or unsubstituted lower alkoxy group, thioether, a substituted piperaz mo group, a substituted ammo group, etc., but it must be substituted with either a cyano or nitro group.
The compounds of the present invention having these combinations of substituent groups have exhibited a high xanthine oxidase inhibiting activity which cannot have been attained by conventional compounds in an in vivo xanthine oxidase inhibiting test, and thus the present inventors have completed the present invention.
1,2,4-triazole compounds as basic structure of the present invention include the following isomers (A), (B), and (C), and all of these are referred to as 1,2,4-triazole and represented by the general formula (1). Isomer (A) [Chemical 3]


Isomer (B) [Chemical 4]



Isomer (C) [Chemical 5]

The process for production of the compound of the present invention is based on a reaction between an iminoether of a corresponding aromatic nitrile with a hydrazide of an aromatic carboxylic acid.
That is, the process is characterized by reacting -an iminoether represented by the corresponding general formula (2): RiCN with a hydrazide represented by the general formula (3): R2CONHMH2 or reacting an iminoether represented by the corresponding general formula (4): R2CN with a hydrazide represented by the general formula (5): RXCONHNH2.
Iminoethers (esters of an imino acid) produced by reacting an aromatic nitrile with an alcohol under basic condition wherein the alcohol may be, for example, sodium methoxide or sodium ethoxide or alternatively with an alcohol such as methanol or ethanol under acidic condition can be used. Such salts produced under acidic condition can be separated and iminoethers produced under basic condition can be separated

as a free compound or may be separated after converted to a salt.
Examples of the solvents usable in the production process of the present compounds include aqueous solvent such as methanol and ethanol, and methanol is preferred. The reaction can proceed at room temperature, but preferably it is conducted under heating in a viewpoint of reaction rate and elimination of water in the reaction. In the case that raethanol is used
as a solvent, temperatures near its boiling point (about 65°C) are preferred, and refluxing condition was adopted in the production examples of the present application. Reaction time can be set freely depending upon the reaction temperature as long as it falls within the range so that side reactions or production of decomposition substances may be suppressed.
In the case that a lower alkyl group substituted with a pxvaloy loxy group is introduced in the compound of the present invention, the reaction can be conducted according to a conventional denydrohalogenation reaction, and is preferably conducted in the presence of a dehydrohalogenation agent such as well-known organic bases and alkalizing agents. The reaction can sufficiently proceed at room temperature and can be conducted in a solvent, an example of which includes dimethylformamide (hereinafter abbreviated as DMF). [Examples]
The compounds and production process according to the present invention and a test method for confirming effectiveness of the compounds and results of the test will

be described more specifically by way of examples. These examples disclosed, however, should not be construed for limiting the present invention.
Dimethyl sulfoxide is hereinafter abbreviated as DMSO in the following examples. Example 1
3-{4-lsobutoxy-3-nitropbenyl)-5-(2-methy1-4-pyridyl) -1,2,4-triazole
1) Production of 4-isobutoxy-3-nitrobenzonitrile
19.5 g of 4-chloro-3-nitrobenzonitrile was dissolved in 200 ml of DMF, and after 16.0 g of 2-methyl-l-propanol, 30 g of potassium carbonate and 7.1g of potassium iodide were
added thereto, the mixture was heated and stirred at 80°C for 24 hours. Water was added to the reaction mixture, which was then extracted with ethyl acetate. The ethyl acetate layer was washed with a saturated saline solution and dried over magnesium sulfate. After the magnesium sulfate was filtered off, solvent was evaporated under reduced pressure and the residue was subjected to silica gel column chromatography. Hexane-ethyl acetate (10:1) was used as an eluent and 5,9 g of the object compound was obtained as a pale yellow crystal.
1 H-NMR(CDC1 »)dppm: 1. 07(6H, d, J = 6. 76Hz), 2. 11-2. 2 5( 1H, m), 3. 94(2H, d, J = 6. 43Hz), 7 . 15(1H, d, J = 8. 91Hz), 7. 8K1H. dd, J*8. 91, 2. 1 5Hz), 8. 14(1H, d, J = 2. 15Hz)
2) 1.54 g of the crystal obtained in 1) was dissolved in 50 ml of methanol, and after 757 mg of sodium methoxide

was added thereto, the mixture was stirred at room temperature under argon atmosphere for 3 hours. 1.06 g of 2-methylisonicotinic acid hydrazide was then added and the mixture was refluxed for 16 hours. After the reaction completed, solvent was evaporated under reduced pressure and water was added to the residue, which was extracted with ethyl acetate, dried over magnesium sulfate. After the magnesium sulfate was filtered off, the solvent was evaporated under reduced pressure. Chloroform was added to the thus obtained residue and stirred at room temperature for one hour. The precipitated solid was filtered, washed with chloroform and dried with a vacuum pump to yield 1.50 g of the object compound as a colorless powder.
1 H-NMR (DMSO-d •) dppm: 1. 0 1(6H, d, J = 6. 60 Hz), 1. 99-2. 15(lH,m), 2. 57(3H, s), 4. 03(2'H , d, J = 6. 43Hz), 7. 56UH, d, J = 8. 91Hz), 7. 80 (1H, d, J = 5. llHz), 7. 88{1H, s), 8. 31(1H, dd, J = 8. 91, 1. 98Hz), 8. 54C1H, d, J = l. 98Hz), 8. 6O(1H, d, J = 5. llHz), 14. 86(1H, s)
Example 2
3-(3-Cyano-4-isobutoxyphenyl)-5-(4-pyridyl)-1,2,4-tr
iazole
1) Production of 4-isobutoxy-3-cyanobenzonitrile 25.2 g of 4-nitrobenzonitrile was dissolved in 300 ml
of DMSO, and after 2 0.0 g of potass ium cyanide was added thereto,
the mixture was heated and stirred at 100cC for one hour. The reaction mixture was cooled to room temperature and after 81.6

g of l-bromo~2-methylpropane and 11.76 g of potassium carbonate were added thereto, the mixture was heated and stirred at 80°C for 8 hours. Water was added to the reaction mixture, which was then extracted with ethyl acetate. The ethyl acetate layer was washed with a saturated saline solution and dried over magnesium sulfate. After the magnesium sulfate was filtered off, the solvent was evaporated under reduced pressure and the residue was subjected to silica gel column chromatog raphy. Hexane-ethyl acetate (3:1) was used as an eluent to yield 21.75 g of a pale yellow powder.
1 H-NMR(CDClO 1 H-NMR (DMSO-d .) 6ppm: 1. 03(6H, d, J = 6. 77 Hz), 2. 03-2. 1 5( 1 H, m), 4. 0 1(2H, d, J = 6. 43H z), 7. 45UH, d, J=8. 74Hz), 7. 99(2H, dd, J = 4 . 45, 1. 65Hz), 8. 29-8. 3 4(2H, m), 8. 73(2H, d d, J = 4. 4 5, 1. 6 5Hz)

The following compounds were prepared according to procedures similar to those in Examples 1 and 2. Example 3
3 - [ 3-Cyano-4 - {{2-methoxy) etftoxymethyl yoxypheny1 ] -5 - ( 2-methyl-4-pyridyl)-l,2,4-triazole Pale brown powder
1 H-NMR (DMSO-d •) 5ppm:2. 57(3H, s), 3. 22(3 H, s), 3. 47-3. 51(2H, m), 3. 8Q-3. 83(2H, ro), 5 . 52(2H, s), 7. 53(1H, d, J = 9. 08Hz), 7. 79{1H, d, J = 4. 9 5Hz), 7. 8 8(1H, s), 8. 3 0-8. 3 5(2H, m) , 8. 6 O(1H, d, J = 4. 9 5Hz)
Example 4
3_[4_(4_Methyl-l-piperazino)-3-nitrophenyl]-5-(2-met hyl-4-pyridyl)-1,2,4-triazole Yellow powder
1 H-NMR (CDCl») Example 5
3-(4-Isobutylamino-3-nitrophenyl)-5-(2-methyl-4-pyri dyl)-l,2,4-triazole Orange powder

1 H-NMR (DMSO-d .) tfppm : 0. 98(6H, d, J = 6. 59 Hz), 1. 94-2. O4(1H, m), 2. 56(1H, s), 3. 17-3. 32(2H, m), 7. 28(1H, d, J = 9. 40Hz), 7. 77-7. 8 7(2H, m)., 8. 1 7(1H, dd, J = 9. 40, 1. 9-8 Hz), 8. 4 3-8. 5812H, m), 8. 81(1H, d, J=l. 98Hz)
Example 6
5- (2-Methyl-4-pyridyl) -3- (3-nitro-4-phenylthiophenyl )-l,2,4-triazole Yellow powder
•H-NMR(DMSO-d.)<2. s h d j="8." m o4> Example 7
3-(4-Isobutylthio-3-nitrophenyl)-5-{2-methyl-4-pyr-id yl)-1,2,4-triazole Yellow powder
1 H-NMR (DMSO-d 0 dppm: 1. 07(6H, d, J = 6. 60 Hz), 1. 9 1-1. 9 6(1H, m), 2. 5 7(3H, s), 3. 0 3(2H , d, J = 6. 76Hz), 7. 80-7. 89(3H,m), 8. 33(1H, dd, J = 8. 41, 1. 98Hz), 8. 61C1H, d, J = 5. 12Hz) , 8. 84(1H, d, J=l. 9 8Hz)
Example 8
5- (2-Methyl-4-pyridyl) -3- (3-nitro-4-phenyltliiomethyl oxyphenyl)-1,2,4-triazole Pale yellow powder

1 H-NMR (DMS0-d.)Sppm:2. 57(3H, s), 5. 95(2 M, s), 7. 2 7-7. 5 1(5 H, m), 7. 71-7. 88(3H, m)» 8 . 33(1H, dd, J = 8. 91, 2. 15Hz), 8. 53-8. 61(2H , m), 14. 92(1H, s)
Example 9
5-(2-Methyl-4-pyridyl)~3-(4-raethylthioHtethyloxy-3-ni trophenyl)-1,2,4-triazole Yellow powder
1 H-NMR (DMS0-d«)£ppm:2. 22(3H, s), 2. 57(3 H, s), 5. 5 8(2H, s), 7. 6 5 — 7. 8 9(3H, m), 8. 31(1 H, dd, J = 8. 41, 1. 65Hz), 8, 54-8. 61(2H, m), 1 4. 94UH, s)
Example 10
3-(4-Benzyloxymethyloxy-3-nitrophenyl)-5-{2-methyl-4 -pyridyl)-1,2,4-triazole Pale yellow powder
1 H-NMR (CDCL) dppm:2. 68(3H, s), 4. 79(2H, s) , 5. 48(2H, s), 7. 30-7. 38{5H, m), 7. 52(1H, d, J = 8. 90Hz), 7. 79(1H, d, J«5. 44Hz), 7. 9O(1H , s), 8. 24(1H, dd, J = 8. 90, 2. 14H2), 8. 57(1H , d, J = 2. 14Hz), 8. 66(1H, d, J = 5. 44Hz)
Example 11
5-(2-Methy1-4-pyridyl)-3-{3-nitro-4-(2-tetrahydropyr anylmethyl)oxyphenyl]-1,2,4-triazole Pale yellow powder

1 H-NMR (DMSO-d •) dppm : 1. 3 5~ 1. 8 1 (6 H, m), 2 . 57(3H, s), 3. 43-4. 26{4H, m), 7. 58(1H, d, J = 8. 91Hz), 7. 8O(1H, d, J = 4. 62Hz), 7. 88(1H, s) , 8. 3O(1H, dd, J = 8. 91, 1. 82), 8. 53(1H, d, J = 1. 82Hz), 8. 60{lH, d, J = 4. 62Hz)
Example 12
5-(2-Cyano-4-pyridyl)-3-(4-pyridyl)-1,2,4-triazole 1) Production of methyl isonicotinate N-oxide 13.9 g of isondcotinic acid N-oxide was added to 209 ml of methylene chloride, 29.7 g of
l-ethoxycarbonyl-2-ethoxy-l,2~dihydroquinoline was further added thereto, and the mixture was stirred under argon atmosphere at room temperature for one hour. 3 2.1 g of methanol was added to this mixture, which was stirred at room temperature for 17 hours. After the solvent was evaporated under reduced pressure, the residue was subjected to silica gel column chromatography. Chloroform-acetone (3:1) was used as an eluent to yield 11.lg of a white powder.
1 H-NMR(CDC Mtfppm: 3. 95(3H, s), 7. 88 2) Production of methyl 2-cyanoisonicotinate 11.1 g of the crystal obtained in 1) was dissolved in 170 ml of acetonitrile, 14.6 g of triethylamine and 21.5 g of trimethylsilylnitrile were added thereto, and the mixture was refluxed under argon atmosphere for 16 hours. After the solvent was evaporated under reduced pressure, the residue was subjected to silica gel column chromatography.

Chloroform-acetone (95:5) was used as an eluent to yield 8.44 g of a pale yellow powder.
1 H-NMR{CDC L)dppm:4. 01{3H, s), 8. O8(1H, d, J = 5. 45Hz), 8. 24(1H, s), 8. 9O(1H, d, J = 5. 45 Hz)
3) Production of 2-cyanoieonicotinic acid hydrazide
8.44 g of the crystal obtained in 2) was added to 85 ml
of methanol, 1.84 g of hydrazine was further added thereto, and the mixture was stirred under argon atmosphere for 2 hours . After the solvent was evaporated under reduced pressure, chloroform was added to the residue, which was stirred at room temperature for one hour. The precipitated crystal was filtered, washed with chloroform and dried with a vacuum pump to yield 4.15 g of a pale yellow powder.
1 H-NMR(DMSO-d ,) 4) Production of the object compound
2.67 g of 4-cyanopyridine was dissolved in 40 ml of methanol, 0.83 g of sodium methoxide was added thereto, and the mixture was stirred at room temperature for one hour. Then 4.15 g of the crystal obtained in 3) was added and the mixture was refluxed for 37 hours. After the reaction completed, the precipitated solid was filtered, washed with methanol and dried with a vacuum pump to yield 3.66 g of the object compound as a yellow powder.

1 H-NMR (DMSO-d .) Example 13
3-(4-Isobutoxy-3-nitrophenyl)-5-(4-pyridyl) -1,2,4-tr iazole
A white powder was obtained according to procedures similar to those in Example 1.
1 H-NMR (DMSO-d «) cSppm: 1. 01(6H, d, J = 6. 60 Hz), 2. 00~2. 12UH, m), 4. 04C2H, d, J = 6. 43H z), 7. 57(1H, d, J = 9. 07Hz), 8. 00(2H, d, J = 6. lOHz), 8. 31(1H, dd, J = 6. 10, 1. 98Hz), 8. 55{ 1H, d, J=l. 98Hz), 8. 74(2H, d, J = 6. lOHz), 14 . 9 2( 1H, s)
Example 14
3-(4-isobutoxy-3-nitrophenyl)-5-{2-methyl-4-pyridyl) -N-pivaloyloxymethyl-1,2,4-triazole
354 mg of the powder obtained in Example 1 was dissolved in 3 ml of DMF, 181 mg of pivaloyloxymethyl chloride and 276 rag of potassium carbonate were added thereto, and the mixture was stirred at room temperature for 18 hours. Ethyl acetate was added to the reaction mixture, which was then washed with water and dried over magnesium sulfate. After the magnesium sulfate was filtered off, the solvent was evaporated under reduced pressure and the residue was subjected to silica gel

column chromatography. Chloroform-acetone (95s5) was used as an eluent and 358 mg of the object compound was obtained as a white powder.
1 H-NMR (CDClO 7. 12-7. 26(2H, m), 7. 49-7. 59(2H, m), 7. 82-
8. 05 H, m)
The following compounds were prepared according to procedures similar to those in Examples 1, 2 or 12. Example 15
3-(4-Butoxy-3-nitrophenyl)-5-(4-pyridyl)-1,2,4-triaz ole Pale green crystal
1 H-NMR (DMSO-d .) tfppm : 1. 34(3H, t, J=7. 29 Hz), 1. 7O(2H, m), 1. 75(2H, m), 7. 6O(1H. d, J = 8. 9 1Hz), 8. 0 0(«ch 2H, d, J = 5. 94Hz), 8. 29(1H, dd, J = 8. 91, 2. 16Hz), 8. 50(1H, d, J = 2. 16Hz) ,8.74(«ach 2H, d, J = 5. 94Hz)
Example 16
5-(4-Isopropoxy~3-nitrophenyl)-3-(4-pyridyl)-l,2,4-t
riazole
Pale yellow crystal

'H-NMR (DMSO-d«) £ppm: 1. 34(3H, d, J = 5. 94H z), 1. 36(3H, d, J = 5. 94Hz), 4. 94UH, m), 7. 60 (1H, d, J = 8. 91Hz), 8. 00{«««=h2H, d, J = 5. 94Hz), 8. 29(1H, dd, J = 8. 91, 2. 16Hz), 8. 50 5-(2-Chloro-4-pyridyl)-3-(4-isobutoxy-3-nitrophenyl) -1,2,4-triazole Brown crystal
1 H-NMR (DMSO-de) Example 18
3-(2-Pyridyl)-5-{3-nitro-4-isobutoxyphenyl)-1,2,4-tr iazole Pale yellow powder
1 H-NMR (DMSO-d.) tfppm: 1. 01(6H, d, J = 6. 76 Hz), 2. O8(1H, m), 4. 02(2H, d, J^6. 43Hz), 7. 5 4(2H, m), 8. 03{lH, t, J = 7. 67Hz), 8. 19(1H, d, J = 7. 92Hz), 8. 31(1H, d, J = 8. 91Hz), 8. 5O(1H , s), 8. 74(1H, d, J = 4. 62Hz), 14. 93(1H, brs)
Example 19
3.(3-Pyridyl)-5-{3-nitro-4-isobutoxyphenyl)-1,2,4-tr iazole Grayish white powder

1 H-NMR (DMSO-d ,) Sppm: 1. 01(6H, d, J = 6. 60 Hz), 2. 08 8. 68(1H, d, J = 3. 79Hz), 9. 25(1H, d, J = 2. 15H
z) Example 20
3-(2-Methyl-4-pyridyl)-5-(3-cyano-4-isobutoxyphenyl) -1,2,4-triazole White powder
1 H-NMR (DMSO-d •) 6ppm: 1. 04(6H, d, J = 6. 76 Hz), 2. 1 1(1H, m), 2. 57(3H, s), 4. 01(2H, d, J = 6. 60Hz), 7. 45(1H, d, J = 8. 58Hz)7. 79(1H, d, J = 5. llHz), 7. 88(1H, s), 8. 3O(1H, d, J = 8. 74 Hz), 8. 33(1H, s), 8. 59(1H, d, J = 5. llHz)
Example 21
3-(2-Methyl-4-pyridyl)-5-(3-nitro-4-methoxyphenyl)-l ,2,4-triazole Yellowish white powder
1 H-NMR (DMSO-d.) Example 22
3- (2-Methyl-4-pyridyl) -5- (3-cyano-4-cyclopropylntetho xyphenyl)-1,2,4-triazole

Pale brown powder
1 H-NMR (DMSO-di)<0. m h s d j> = 7. 09Hz), 7. 44(1H, d, J = 8. 60Hz), 7. 79(1H, d, J = 5. llHz), 7. 88(1H, s), 8, 31(1H, d, J = 9. 07Hz), 8. 33(1H, s), 8. 59UH, d, J»5. llHz)
Example 23
3-(2-Cyano-4-pyridyl>-5-(2-methyl-4~pyridyl)-1,2,4-t
riazole
Pale yellow powder
1 H-NMR (DMSO-d«) Example 24
3-(2-Methyl-4-pyridyl)-5-[3-eyano-4-(4-methoxyfeenzyl oxy)phenyl3-1,2,4-triazole White powder
1 H-NMR (DMSO-d ,) Example 25
3-(2-Methyl-4-pyridyl)-5-(3-cyano-4-isopentyloxyphen yl)-l,2,4-triazole

White powder
1 H-NMR (DMSO-d .) £ppm : 0. 97(6 H, d, J = 6. 60
Hz), 1. 70(2H, m)» 1. 84(1H, m), 2. 57(3H, s), 4. 26(2H,t, J = 6. 52Hz), 7. 48(1H, d, J=8. 58Hz), 7. 7 9(1H, d, J = 4. 78Hz), 7. 8 8(1H, s ), 8. 3 2(1H , dd, J = 2. 31, 8. 58Hz), 8. 33(1H, s), 8. 59(1H , d, J = 4. 78Hz), 14. 80(111, brs)
Example 26
3-{2-Methyl-4-pyridyl)-5-(3-cyano-4-methoxyphenyl)-1 ,2,4-triazole Brown powder
1 H-NMR (DMSO-d .) dppm:2. 57(3H, s), 4. 01(3 H, s), 7. 47(1H, d, J = 5. 77Hz), 7. 88(1H, s), 8. 3 5(2H, m), 8. 59(1H, d, J = 5. 28Hz)
Example 27
3-(2-Chloro-4-pyridyi)-5-(2-methyl-4~pyridyl)-1,2,4-triazole Pale brown powder
1 H-NMR (DMSO-d 0 <7. d j="5." hz s o2 llhz lhz> Example 28
3-{2-Methyl-4-pyridyl)-5-(3-cyano-4~propargyloxyphen yl)-1,2,4-triazole Pale brown powder

H-NMR (DMSO-d .) 6ppm: 2. 57(3H, s), 3. 76(1 H, s), 5. 12(2H, d, J = l. 81Hz), 7. 52{1H, d, J =
8. 41Hz), 7. 79(1H, d, J = 5. 6 1), 7. 88(1H, s), 8 . 36(1H, d, J = 8. 25Hz), 8. 37(1H, s), 8. 60 Example 29
3-(2-Methyl-4-pyridyl)-5-[3-cyano-4-ethoxy)ethoxy}phenyl3-1,2,4-triazole White powder
1 H-NMR (DMSO-d.) 5ppm:2. 79(3H, s ), 3. 59-3 . 72(8H,m), 3. 8 5(2H, m), 4. 4 0(2H, m), 7. 5 3(1H , d, J = 8. 91Hz), 8. 3K1H, d, J = 5. 28Hz), 8. 38 (1H, dd, J = 1. 98, 8. 9 IHz), 8. 43(2H, brs), 8. 8 3(1H, d, J = 6. 1 OHz)
Example 30
3-(2-Isobutylthio-4-pyridyl)-5-(3-nitro-4-isobutoxyp henyl)-1,2,4-triazole Yellow powder
lH-NMR (CDCL) dppm: 1. 06(6H, d, J«6. 60Hz), 1. 08(6H, d, J-5. 61Hz), 1. 9 9
Example 31
3-(2-Methyi-4-pyridyl)-5-(3-cyano-4-methoxyethoxyphe nyl) -1,2,4-triazole White powder
1 H-NMR (CDCL) tfppm: 2. 65(3H, s), 3. 5 1(3H, s) , 3. 8 7 Example 32
3-(2-Methyl-4-pyridyl)-5-[3~cyano-4-{(2-methoxyethox y)ethoxy}pheny1]-1,2,4-triazole White powder
'H-NMR (CDCL) tfppm:2. 66(3H, s), 3. 42(3H, s) , 3. 66{2H, m), 3. 84(2H, m), 3. 96(2H, m), 4. 2$( 2H,m), 7. 0 1( 1H, d, J = 8. 91Hz), 7. 7 8( 1H, d, J = 5. 28Hz), 7. 89(1H, s), 8. 19(1H, dd, J = 2. 31 , 8. 74Hz), 8. 26UH, d, J = 2. 14Hz), 8. 63(1H, d, J = 5. 1 IHz)
Example 33
3-(2-Methyl~4-pyridyl)-5-[3-cyano-4-{( xy>ethoxy)ethoxy}pheny1]-1,2,4-triazole White powder

1 H-NMR (DMSO-d .) dppm:2. 8 1(3H, s), 3. 23(3 H, s), 3. 4 3(2H, m), 3. 51-3. 5 7(4H, m), 3. 65(2 H, m), 3. 84(2H, m), 4. 39(2H, m), 7. 5 3( 1H, d, J = 8. 9lHz), 8. 33(1H, d, J = 6. 02Hz), 8. 3 9(1H, d, J = 8. 91Hz), 8. 44(2H, s), 8. 84(1H, d3 J = 6. 02Hz)
Example 34
3-(2-Methoxy-4-pyridyl)-5-{3-nitro-4-isobutoxyphenyl )-l,2,4-triazole Yellow crystal
1 H-NMR (DMSO-d .) fippm: 1. 0 K6H, d, J = 6. 76 Hz), 2. O8(1H, m), 3. 92(3H, s), 4. 03(2H, d, J = 6. 43Hz), 7. 4O(1H, s), 7. 55(1H, d, J = 8. 74Hz) , 7. 61(1H, d, J = 5. 77Hz), 8. 3O(1H, dd, J=2. 1 5, 8. 75Hz), 8. 32(1H, d, J = 5. HHz), 8. 5 3(1.H , d, J=l. 98Hz), 14. 88(1 H, brs)
Example 35
3-(2~Cyano-4-pyridyl)-5-(l-oxy-4-pyridyl)-1,2,4-tria zole Yellow powder
1 H-NMR (DMSO-d .) dppm:8. 0 1(2H, dd, J = l. 9 8, 5. 36Hz), 8. 2 9( 1H, dd, J= 1. 65, 5. 1 1 Hz), 8 , 40(2H, dd, J = l. 98, 5. 36Hz), 8. 52(1H, d, J = 1. 65Hz), 8. 92(1H, dd, J = l. 65, 5. llHz)
Example 3 6
3_(2-Cyano-4-pyridyl)-5-[3-cyano-4-{(2-methoxyethoxy )ethoxy}phenyl]-l,2,4-tria2ole

Pale yellow powder
'H-NMR (CDCL) (5ppm:3. 41(3H, s), 3. 60(2H, m) , 3. 79(2H, m), 3. 97(2H, m), 4. 35(2H, m), 7. 18( 1H, d, J = 7. 24Hz), 8. 24-8. 28(3H, m), 8. 45(1 H, s), 8. 8 1(1H, d, J = 5. 28Hz)
Example 37
3-(2-Cyano-4-pyridyl)-5-{2-chloro~4-pyridyl)-1,2,4-t riazole Pale yellow powder
1 H-NMR (DMSO-d ,) (5ppm : 8. 0 2( 1 H, d, J=5. 11 Hz), 8. 0 8( 1 H, s), 8. 31(1H, dd, J = 1 . 65, 5. 11 Hz), 8. 55(1H, s), 8. 63(1H, d, J = 5. llHz), 8. 9 4(1H, d, J = 5. 1 lHz)
Example 3 8
3-(2-Cyano-4-pyridyl) -5- (2-phenyl-4-pyridyl) -1,2,4.-t
riazole
Pale yellow powder
1 H-NMR (DMSO-d •) Example 39
5-(2-Cyano-4-pyridyl)-3-(4-pyridyl)-1,2,4-triazole
1) Production of isonicotinic acid (N2-tert-butoxycarbonyl) hydrazide-1-oxide
585 ml of methylene chloride was added to 39.0 g of isonicotinic acid N-oxide, and after 34.0 g of triethylamine

was further added thereto, the mixture was cooled under argon atmosphere to -15°C. 33.5 g of ethyl chlorocarbonate in 117 ml of methylene chloride was added dropwise to this mixture, which was stirred at a temperature from -P5 to -10°C for one hour. Then 4 4.4 g of tert-butyl ester of carfoagzic acid in 117 ml of methylene chloride was added dropwise to this mixture and it was allowed to slowly rise to room temperature while it was stirred. The precipitated solid was filtered after 15 hours, washed with methylene chloride, and dried with a vacuum pump to yield 4 9.7 g of white crystal.
1H-NMR(DMSO - dS)6 ppm: 1.42 ( 9H , s ) , 7.82 { 2H , d , J=7.09Hz ) , 8.33 ( 2H , d, J=7.09Hz ) , 9.02 ( 1H , s ) , 10.44 ( 1H , s )
2 ) Production of 2-cyanoisonicotinic acid hydrazide 1 /% p-toluenesulfonic acid salt
228 ml of dioxanewas added to 30.4 gof the crystal obtained in 1), and after 13.1 g of trimethylsilyl cyanide and 38.8 g of N, N-dimethy lcarbamoy 1 chloride were further added thereto,
the mixture was stirred under argon atmosphere at 60°C for 5 hours. After the solvent was evaporated under reduced pressure, the residue was dissolved in ethyl acetate and subsequently washed with 1.5 H sodium carbonate aqueous solution and a saturated saline solution and dried over magnesium sulfate. After the magnesium sulfate was filtered off, the solvent was evaporated under reduced pressure. Ethyl acetate was added to the residue, 68.5 g of p-toluenesulfonic ac id monohydrate was added thereto, and the mixture was stirred at room temperature for 22 hours. The precipitated crystal

was filtered, washed with ethyl acetate, and dried with a vacuum pump to yield 40.3 g of white crystal 2),
1H - NMR(DMS0 - d6) 6 ppm: 2.28 ( 4.5H , s ) , 7.12 ( 3H , M , J=7.92 & 0.66Hz } , 7.48 ( 3H , dd , J=7.92 & OMEZ ) , 8.10 ( 1H , dd , «J=5.11 & I.8IH2 ) , 8.39 ( 1H , dd , J=1.81 & 0.33Hz ) , 8.99 ( 1H , dd , ,1=5.11 & 0.33Hz )
3) Production of
5-(2-cyano-4-pyridyl)-3-(4-pyridyl)-1,2,4-triazole
9.98 g of 4-cyanopyridine was dissolved in 250 ml of methanol, and after 7 .77 g of sodium met hoxide was added thereto, the mixture was stirred at room temperature for one hour. Then 40.3 g of the crystal obtained in 2} was added and the mixture was refluxed for 24 hours. After the reaction completed, the precipitated crystal was filtered, washed with methanol, and dried with a vacuum pump to yield 16.3 g of yellow crystal. 1H - NMR(DMSO - d6) 6 ppm: 8.01 { 2H , dd , J=4 54 & 1.57H* ) , 8.31
( 1H , dd , J=5.11 & 1.65Hz ) , 8.53 ( 1H , dd, J-1.65 & O.SOHz ) , 8.80 { 2H , dd , J=4.54 & 1.57Hz ) , 8.93 ( 1H , dd , J=5.11 & O.SOHs )
4) Production of
5-(2-cyano-4-pyridyl)-3-{4-pyridyl)-1,2,4-triazole
45 ml of ethanol and 15 ml of 1-methyl-2-pyrrolidone were added to 3.0 g of the crystal obtained in 3), and the mixture
was heated and stirred at 80°C for 19 hours. The crystal was filtered, subsequently washed with a mixture of ethanol and 1-methy1-2-pyrrolidone (3:1) and ethanol, and dried with a vacuum pump to yield 2.71 g of yellow crystal-

5) Production of
5-{2-cyano-4-pyridyl)-3-(4-pyridyl)-1,2,4-triazole p-toluenesulfonic acid salt
5 ml of ethanol and 30 ml of water were added to 2.4 8 g of the crystal obtained in 4), and after 3.8 g of p-toluenesulfonicacidmonohydratewas further added thereto, the mixture was stirred at room temperature for 5 hours. The precipitated crystal was filtered, subsequently washed with a mixture of ethanol and water (1:6), water and then ethanol, and dried with a vacuum pump to yield 3.5 g of white crystal.
1H-NME(DMSO - d6) 6) Production of the object compound
17 ml of ethanol and 17 ml of water were added to 3.36 g of the crystal obtained in 5), and the mixture was stirred at room temperature for 30 minutes. A solution of sodium carbonate (0.74 g of sodium carbonate in 17 ml of water) was further added, and the mixture was stirred at room temperature for 2 hours. The precipitated crystal was filtered, subsequently washed with water and ethanol, and dried with a vacuum pump to yield 1.89 g of the object compound as a pale yellow crystal. Test Examples
Method for evaluating the effect of lowering serum uric acid level in vivo

Each of test compound was suspended in a 0.5% methylcellulose (MC) aqueous solution and administered orally to seven-week-old male Wistar rats (4 rats per group) compulsorily through a feeding tube at a dose of 0.3 mg/5 ml/kg (1 mg/5 ml/kg for Example 17 and Compound 44 of J. Medicinal Chemistry, (1975) as a control). The blood was collected from the orbital sinus at 6 hours after administeration of the test compound. Each blood sample was allowed to clot for one hour and then centrifuged at 2000xg for 10 minutes to obtain serum. The uric acid level in the serum was determined using a kit (Wako Pure Chemicals Industries, Ltd.; phosphotungstic acid method) and a reduction rate of serum uric acid level was calculated by the following formula:
Reduction rate of serum uric acid level = (1-Average serum uric acid level in the group receiving a test compound/Average serum uric acid level in the group receiving MC) x 100

[Table 1]
Reduction rate of serum uric
acid level
Example 1 66.5%
Example 2 62.3%
Example 3 40.0%
Example 4 43.9%
Example 5 39.9%
Example 6 40.6%
Example 7 42.7%
Example 8 32.5%

Example 9 35 .7%
Example 10 41 .6%
Example 11 41 .6%
Example 12 51 .1%
Example 13 46 .8%
Example 14 43 .0%
Example 15 41 .2%
Example 16 36 .1%
[Table ; 2]
Reduction rate of serum
urxc acid level
Example 17 40. .6%
Example 20 67. .5%
Example 21 52. ,9%
Example 22 32. .6%
Example 23 41. 1%
Example 24 38. 3%
Example 25 47. 2%
Example 26 37. 9%
Example 27 32. 9%
Example 28 35. 3%
Example 29 40. 7%
Example 31 36. 5%
Example 32 52. 0%
Example 33 44. 7%
Example 35 46. 8%
Example 37 33. 6%
Positive control
Japanese Patent Publication No. 49-46622 (Compound of
Example 3) -11.1%

Japanese Patent Publication No. 50-24315 (Compound of
Example 2) 2 6.1%
Japanese Patent Publication No. 50-24315 (Compound of
Example 1) -0.4%
J. Medicinal Chemistry, 1975, Vol. 18, No. 9 (Compound
44) -7.7%
1,2,4-Triazole compounds which have a high xanthine oxidase inhibiting activity and are useful as therapeutic agents for hyperuricemia and gout due to hyperuricemia caused by increased production of uric acid have been provided by selecting the compounds represented by the general formula (1) according to the present invention.

Documents:

848-delnp-2004-abstract.pdf

848-delnp-2004-assignment.pdf

848-delnp-2004-claims.pdf

848-delnp-2004-correspondence-others.pdf

848-delnp-2004-correspondence-po.pdf

848-delnp-2004-description (complete).pdf

848-delnp-2004-form-1.pdf

848-delnp-2004-form-18.pdf

848-delnp-2004-form-2.pdf

848-delnp-2004-form-3.pdf

848-delnp-2004-form-5.pdf

848-delnp-2004-gpa.pdf

848-delnp-2004-pct-210.pdf

848-delnp-2004-pct-301.pdf

848-delnp-2004-pct-304.pdf

848-delnp-2004-pct-306.pdf

848-delnp-2004-pct-345.pdf

abstract.jpg


Patent Number 217753
Indian Patent Application Number 848/DELNP/2004
PG Journal Number 17/2008
Publication Date 25-Apr-2008
Grant Date 28-Mar-2008
Date of Filing 01-Apr-2004
Name of Patentee FUJI YAKUHIN CO. LTD
Applicant Address 4-383, SAKURAGI-CHO, OMIYA-KU, SAITAMA-SHI, SAITAMA 330 0854 JAPAN.
Inventors:
# Inventor's Name Inventor's Address
1 NA NA
2 SATO, TAKAHIRO 804, Berumezon Akabane, 1-30-11, Akabanenishi, Kita-Ku,
3 ASHIZAWA, NAOKI 1-302, 1-1-10, Naka, Kamifukuoka-shi, SAITAMA 3560025 JAPAN
4 MATSUMOTO, KOJI B-205, 485, Sashiogi, Saitama-shi SAITAM 331-0047 JAPAN
5 IWANAGA, TAKASHI 170-3, Raiha, Kazo-shi SAITAMA 347-0044,JAPAN
6 INOUE, TSUTOMU 1-8-2-201, Futawanishi, Funabashi-shi,CHIBA 274-086 JAPAN
7 NAKAMURA, HIROSHI 5-690-10, MATSUGAOKA, NAGAREYAMA-SHI, CHIBA 270-0141, JAPAN.
8 KANEDA, SOICHI 3-19-5, Saiwai-cho, Shiki-shi, Saitama 353-0005, JAPAN.
PCT International Classification Number C07D 401/14
PCT International Application Number PCT/JP02/12662
PCT International Filing date 2002-12-03
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 017825/2002 2002-01-28 Japan