Title of Invention	AN INGESTIBLE COMPOSITION AND METHOD FOR PROLONGING THE USEFUL LIFE OF ENTERAL FEEDING TUBES
Abstract	An ingestible composition to prolong the useful life of an enteral feeding tube and a system of an enteral feeding tube and the composition is provided. The complete enteral feeding solution contains an edible antimicrobial agent which is 0.05% to 1.0% by weight alkyl paraben, the alkyl group having 1- 10 carbon atoms. Methyl, ethyl, or propyl paraben are particularly preferred.

Full Text

COMPOSITION AND METHOD FOR PROLONGING THE USEFUL LIFE OF ENTERAL FEEDING TUBES This Invention relates to methods and compositions for prolonging the useful life of eternal feeding tubes. More particularly, the invention relates to a method and composition for prolonging the useful life of an enteral feeding tube while the tube remains inserted in a patient. In a further respect, the invention relates to an enteral feeding composition which can be safely ingested by a patient, but which inhibits microbial growth. BACKGROUND The administration through enteral feeding tubes of aqueous solutions of nutritionally balanced food compositions is known in the art. Enteral feeding formulas and devices can provide favorable environments for contamination and support of growth for microorganisms, especially when the materials are not properly handled or managed. The formulas provide medium and nutrients necessary to support growth, and the devices provide ambient conditions (e.g., surfaces, warm temperatures, etc.) which can accelerate the colonization of the microorganisms. As a consequence, any errant contamination of the enteral feeding system can lead to a significant rise in contaminants unless proper care and precautionary measures are taken; even so, contamination of enteral feeding systems continues to be problematic in health care facilities. During the use of enteral feeding tubes, residual food proteins, starches, and other components often accumulate in and may block the feeding tube. Such accumulation and blockages provide a ready site for the growth and multiplication of microorganisms. Cleaning of feeding tubes may be performed by flushing with water and in some cases enzymes (e.g., meat tenderizer) or other agents that may dissolve residual food blockages or accumulations. However, cleaning is usually incomplete and tubes eventually may become partially or completely occluded, or the tube material may deteriorate and fail. Accordingly, it would be highly desirable to provide a method for reducing the incidence of, or preventing blockages and material degradation in an enteral feeding tube that would maintain and prolong the useful life of the feeding tube and avoid tube replacement. Many different types of microorganisms have been identified in enteral feeding systems and have been found in the formula container, the delivery set, and the patient's feeding tube (and stomach). Among those identified are yeasts and fungi such as Aspergillus’s niter and Candid a alb cans. Candid has commonly been found to be present in contaminated feeding systems and has been associated with severe degradation of feeding tubes. The source of the contamination is likely either the patient (stomach) or the caregiver (hands). Feeding tubes may deteriorate to the point of clogging due to microorganism overgrowth, material breakdown and discoloration. In some cases, patient (nosocomial) infection has been attributed to contaminated feeding systems. Risks associated with microorganism contamination include overgrowth and clogging of patient feeding tubes, deterioration of feeding tube materials, curdling of formula in feeding containers/systems, and possible patient local site infection and source for nosocomial infections. Reducing or inhibiting the proliferation of microorganisms in the enteral-feeding formulas by use of various preservative methods has been applied in some commercial mixtures such as Vivien™ (Novelties) and AMTF™ (Nye). These methods have employed the fact that low pH ( formulas where maintaining is necessary to avoid protein denaturalization and formula curdling. There are a number of food additives that display ant microbial properties and are effective in inhibiting the growth of certain microorganisms. These additives include alkyl parabens (p-hydroxybenzoic acids), alkyl having 1-10 carbon atoms, including methyl and propyl parable; also useful are benzoic acid and its derivatives, sorbet acid and its derivatives, prop ionic acid and its derivatives, lactic acid and its derivatives, alkyl carbonates, bisulfate’s, Nissan, natamycin, alkyl sulfates and others. Some of these additives have been used individually and in combination to inhibit microbial growth in liquid and solid foods, but they have not been used in feeding tube compositions to inhibit the growth of microorganisms and to prevent clogging of the tube. Therefore, it is a principal object of the invention to provide an improved method and composition for prolonging the useful life of an enteral feeding tube. Another object of the invention is to provide a composition, which inhibits microbial growth in a feeding tube formula. A still further object of the invention is to provide an enteral feeding tube system comprising a feeding tube and an enteral feeding tube microbial growth inhibition formula, which has prolonged life, does not utilize toxic cleaning agents and which can safely be ingested by a patient. These and other further and more specific objects and advantages of the invention will be apparent to those skilled in the art from the following detailed description. SUMMARY OF THE INVENTION To minimize or reduce fungal, yeast and other microorganism proliferation in patient feeding tubes and thereby extend the useful life of the enteral feeding system, this invention teaches the addition of the antimicrobial parabens (preferably lower alkyl having 1-10, or 1-5 carbon atoms; especially the methyl, ethyl or propyl, or hefty parabens) to enteral formulas and delivery of the antimicrobial mixture to the lumen of a patient's feeding tube (as during normal continuous feeding) on a continuous or intermittent basis for this purpose. Furthermore, this invention demonstrates that lower alkyl, especially methyl and propyl parabens in effective inhibitory concentrations up to 1.0% will inhibit the growth of fungus, yeast and other microorganisms such as lactobacillus) and prolong the time that feeding tubes will remain patent when exposed to contamination in the formula. The use of the invention system of antimicrobial enteral formula together with a feeding tube, is also useful in reducing the deterioration of feeding tube materials, per se, by reducing and/or limiting the influence that microorganism overgrowth may have on the degradation process. The precise ingredients of the enteral diet composition aspect of the present invention are not critical to the practice of the invention. Any suitable and commercial available diet can be employed. A complete feeding tube diet typically includes vitamins, minerals, trace elements as well as nitrogen, carbohydrate and fatty acid sources in a liquid form so that it can be used as the sole source of nutrition supplying essentially all the required daily amounts of vitamins, minerals, carbohydrates, fatty acids and the like. Any diet composition, whether as nutritional supplement or a total feeding, provides an energy supply of from about 10 to 3500 kcal/day, preferably 100-2000 kcal/day, and more preferably from about 150 to about 2000 kcal/day; and still more preferably 250-1500 kcal/day. Within the parameters of Table 1, there are many suitable complete enteral feeding formulas useful in this invention. With reference to the components listed in Table 1, suitable nitrogen sources include proteins such as castigates or protein hydrolysates or vegetable protein such as soy and amino acids, or combinations thereof. Suitable carbohydrate sources include various starches and maltodextrins. Carbohydrates can be selected from among the group consisting of digestible carbohydrates such as dextrose, fructose, sucrose, maltose, corn syrup solids, disaccharides, tetrasaccharides, pentasaccharides, hexasaccharides, oligosaccharides and high saccharine, or mixtures thereof. Suitable fat sources include the triglycerides. The standard enteral formula composition of Table 1 contains water, which makes the composition liquid enough to be used in the feeding tube situation. These compositions can also be supplied in the powered form that may be reconstituted with the water to make up the liquid. The powder is ordinarily partially dissolved and partially suspended in the resulting liquid form of the invention. While it is possible to reconstitute the composition with liquid such as alcohol, the reconstituting liquid will ordinarily be water. The water may contain additional ingredients such as alcohol, glycerol, propylene glycol, sugars and flavor. Edible acidulates can also be utilized in the powder composition of the invention, and include malice acid, acetic acid, citric acid, lactic acid, numeric acid, ascorbic acid, or an acidic salt such as sodium acetate in order to adjust the pH within the range of 2 to 6.9. An acid pH is useful because it reduces microbial activity and increases the effectiveness of some antimicrobial agents. In a preferred embodiment of this invention, however, the pH can be from 3-8, and preferably pH>5.5, in order to avoid protein denaturalization and formula curdling. When the powder rejuvenation composition of the invention is reconstituted with water, the amount of water used with a selected weight of powder can vary as desired. When, for example, it is desired to make the resulting aqueous solution more viscous so it better adheres to the internal surfaces of the feeding tube and to residual food in the feeding tube, the quantity of water can be reduced or the amount of fiber increased. When it is desired to increase the viscosity of the solution that results after the powder composition is reconstituted with water, xanthenes gum, carrageen an or another thickener can be included in the powder composition. Such thickeners are normally present in the powder rejuvenation composition in a concentration in the range of 0,50% to 12.0% by weight. The preferred viscosity of the reconstituted enteral feeding tube composition will, as appreciated by those of skill in the art, vary depending on a number of factors. Such factors can include the size of the enteral tube, the food composition being administered through the tube, whether the tube is completely blocked, whether there is a large amount or a small amount of residual food, whether the reconstituted rejuvenation composition is administered with a food composition, etc. Even at low viscosities of 50 centipoises or less, the composition retains its homogeneity; the preferred viscosity is usually less than 50 centipoises. Enteral tubes are used to dispense food into the esophagus, stomach, or intestinal tract of a patient. The alkyl parabens, when alkyl is defined as having an 1-10 carbon atoms, preferably 1-5 carbon atoms, and most preferably methyl, ethyl, or propyl, can be present in the enteral feeding composition at from about 0.1% to about 1.0%, by weight based on the total weight of the feeding composition in its final liquid (or reconstituted) form. Other antimicrobial agents can be employed in this invention, including benzoic acid salts and esters; sorbet acid salts and esters; propionic acid salts and esters; lactic acid salts and esters; alkyl carbonate (alkyl having 1-5 carbon atoms, such as dimethyl carbonate; bisulfate salts; nosing; natamycin and pyramiding; spooning; and dactyl. The use of the term "salts" is defined as being the pharmaceutically acceptable salts, such as sodium, potassium, or the like. These will be useful at about the same levels as that of the alkyl parabens. The following Examples illustrate the invention. Example 1: Paraben challenge in commercially sterile product. Commercially sterile product (Is source®) with 0.1% methyl or propyl parable is prepared. Organisms were challenged in both variations at their optimum growth temperature and room temperature (RT). The populations are determined at different time intervals and compared to a spiked control without the parabens. At 35- C, Staphylococcus auras shows slightly lower levels than the spiked control (no parapet) at 24 hours but by 48 hours no inhibition is seen. At ROOM TEMPERATURE, both parabens yield lower levels than the control at 35C. Inhibition of Nitrobacteria cloacae at 24 hours looks dramatic with methyl parapet at 35-C, but .5 logs difference in population is seen at 48 hours compared to the control. At room temperature, methyl paraben shows slight inhibition at 24 hours and increases by 48 hours with growth 1.5 logs lower than the propyl paraben sample. At 24 hours, Lactobacillus chuan//levels are close to original inoculum’s levels. At 48 hours, both parabens are inhibitory at room temperature and 35-C at levels 3-5 log cycles below the spiked control without paraben. Aspergillums is inoculated at 530 colony forming units (CFU)/mL This population could not be recovered from the samples containing paraben at all. A few CPU's were seen at 7 days but the only significant growth occurred at 14 days in the spiked sample without paraben. Candid alb cans is inhibited by propyl paraben at 7 days showing a 2-3 log cycle lower CPU/ml than the control, but by 14 days, this difference narrows to EXAMPLE 2: A Challenge Study Using Combinations A challenge study is done on commercially sterile product containing combinations of 4 GRAS antimicrobial agents: methyl paraben, propyl paraben, dactyl, and natamycin. Ten different matrices are tested against 6 different organisms: A. Niger, B. stearotherm, C. alb cans, E. cloacae, L Leichmaml and S. aurous. The most effective inhibitory agent overall is 0.2% propyl paraben. It shows good inhibition of three out of the six organisms studied, and slight inhibition against S. auras, on which no other agent had any effect. Methyl paraben alone was very effective against two organisms {Aspergillus’s and Bacillus), and slightly inhibitory on three others. The combination of methyl paraben & propyl paraben is as effective as one alone. The two other antimicrobials (dactyl and natamycin) are not as effective alone as the parabens. When they are in combination with one of the parabens, inhibition was equal to or less than the paraben by itself. Dactyl or natamycin does not contribute any synergistic effect. Results on the organisms are summarized as follows: Aspergillums nicer 0.2 % methyl paraben and 0.2% propyl paraben are most effective against growth, whereas both parabens at 0.1% each was only mildly effective. Bacillus stearothermoDhilus The control has lower growth levels at 24 hours than the test samples, but methyl paraben, and a combination of both parabens, showed the greatest amount of inhibition. Other agents are only slightly effective in slowing growth. Candida abacas 0.2% propyl paraben shows good inhibition. The combination of 0.1% methyl paraben and 0.1% propyl paraben, and 0.2% methyl paraben has a slight inhibitory effect. Enterobacter cloacae The test composition with 0.2% methyl paraben slows growth slightly in the first 24 hours, but otherwise growth is uninhibited. Lactobacillus leichmanni 0.2% methyl paraben and 0.2% propyl paraben are effective. Staphylococcus aurous 0.2% propyl paraben shows a slight inhibitory effect in the first 24 hours. EXAMPLE 3 Test demonstrating increased lifetime of feeding tube (in vitro) when enteral feeding solution has 0.1% propyl paraben vs. no paraben. Feeding solution is perused through a feeding tube and maintained at approximately 30°C during the test, which may last several days or weeks. At the time the test is begun the solution within the feeding tube is inoculated with 100 CFU of Candida abdicants. After a few hours of incubation (no flow in the tube), perfusion is started and continued until the tube becomes clogged due to overgrowth of the microorganisms. Graph 1 illustrates the results of this test wherein 0.1% propyl paraben containing formula extended the time when clogging occurred by 1 to 2 weeks beyond that attained with no paraben. EXAMPLE 4 The antimicrobials, propyl paraben, sodium benzoate, and potassium sorbet are challenged singly, and in combinations, against 5 organisms. Results are consistent within the same challenge organism trial, and inhibition using a combination of all three antimicrobials is also effective on the variety of organisms tested. Is source HN® is packaged and retorted with the allowable FDA limits. Three samples. A, B, or C are prepared, having, respectively, 0.13% propyl paraben (A), 0.1% sodium benzoate (B), and 0.1% potassium sorbate (C). Additional samples D, E, and F are mixtures of A & B, A & C, and A & B & C. Three control samples: G having 0.26% ethanol; H having no preservatives and I, a blank control are also prepared. A 350-ml aliquot of each retorted product is transferred to a sterile flask and inoculated with one of the designated organisms. A like trial is initiated for each of five organisms, Aspergillums nicer, Candida abdicants, Enterobacter cloacae, Staphylococcus aureus, and Lactobacillus delbrueckii. These organisms are inoculated, respectively, at 44 organisms/ml product; 2 organisms/ml product; 2 organisms/ml product; 4.1 organisms/ml product, and 30 organisms/ml product. An ethanol control is included for all the tests because it is used as the solvent carrier for paraben. An inoculated control with no inhibitors is also used for comparison. The samples are incubated at the appropriate temperature for optimum growth, and aliquots are pulled for testing at the designated intervals. Results are read and recorded as the population per milliliter of sample tested. Results of the Specific Organism Test Runs as follows: 1. Aspergillums Niger growth is inhibited most by propyl paraben than the other antimicrobials. At 7 days, only propyl paraben samples do not exhibit spore growth, and at 10 days, spores are visible on all samples. When spores formed, the sample is noted as "overgrown" and no counts are done, since spore growth compromises exponential growth and skews the data. The ethanol promotes growth in this test, as the inoculated control with no inhibitors have a count 4+ log cycles lower than the inoculated ethanol control at three days. 2. Candida barbicans is inhibited most by the combination of all 3 antimicrobials. The ethanol also stimulates the yeast, as evidenced by a count 3 log cycles greater than the inoculated blank control after 3 days, Potassium sorbate alone is not effective, but in conjunction with propyl paraben, results are lower than propyl paraben by itself. 3. Enterobacter cloacae is inhibited most by the combination of all 3 antimicrobials. Potassium sorbate is the most effective agent alone, and more effective with propyl paraben than with sodium benzoate. Two inoculated controls (ethanol and blank) coagulated before the end of the trial and are not tested further. 4. Staphylococcus aureus shows the greatest inhibition of the 3 bacteria with the combination of all three antimicrobials. There is only a 3 log cycle increase in the population over the 48 hour period. This organism also exhibits some inhibition from the ethanol control. 5. Lactobacillus delbrueckii exhibits inhibition at 24 hours very similar to the other bacteria, but at 48 hours, there is less than Vs. log cycle difference between all the populations. The inoculated control (with no inhibitors) is in the death phase at 48 hours, as the population peaks between 24 and 48 hours, having a decreased count at 48 hours. All the other counts are still increasing at 48 hours, with the combinations of inhibitors showing a slightly better effect than any substance used alone. Propyl paraben by itself is more effective against 4 of the organisms than either of the other 2 antimicrobials alone. The combination of any 2 inhibitors is more effective than any of them used singly. Overall, the combination of all 3 antimicrobials is the most effective in 4 of the five challenges demonstrating an inhibitory synergism. WE CLAIM: 1. An ingestible composition for prolonging the useful life of an enteral feeding tube, comprising enteral complete feeding solution and from 0.05 to 1.0% by weight alkyl paraben wherein the alkyl group has 1 to 10 carbon atoms. 2. The composition as claimed in Claim 1 having a pH of 3-8. 3. The composition as claimed in Claim 1 or Claim 2 in which the alkyl paraben is selected from the group consisting of methyl paraben, ethyl paraben and propyl paraben, and is preferably propyl paraben. 4. The composition as claimed in any of Claims 1 to 3 in which the alkyl paraben is used at 0.1-0.2% by weight. 5. The composition as claimed in any of Claims 1 to 4 in which the alkyl paraben is combined with 0.1-0.2% by weight of pharmaceutically acceptable salts of benzoic or ascorbic acids. 6. The composition as claimed in Claim 5 in which the alkyl paraben is combined with sodium or potassium benzoate or orate. 7. An enteral feeding tube system having a prolonged lifetime, comprising an enteral feeding tube and an ingestible complete enteral feeding solution, said solution containing from 0.05 to 1.0% by weight alkyl paraben wherein the alkyl group has 1 to 10 carbon atoms. 8. The system as claimed in Claim 7 in which the pH of the enteral feeding solution is 3-8. 9. The system as claimed in Claim 7 or Claim 8 in which the alkyl paraben is used at 0.1-0.2% by weight. 10. The system as claimed in any of Claims 7 to 9 in which the alkyl paraben is selected from the group consisting of methyl paraben, ethyl paraben, and propyl paraben, and is preferably propyl paraben. 11. The system as claimed in any of Claims 7 to 10 in which the alkyl paraben is combined with 0.01-0.5% by weight of pharmaceutically acceptable salts of benzoic or sorbet acids. 12. The system as claimed in Claim 11 in which the alkyl paraben is combined with 0.1-0.2% by weight of pharmaceutically acceptable salts of benzoic or sorbet acids. 13. The system as claimed in Claim 11 or Claim 12 in which the alkyl paraben is combined with sodium or potassium benzoate or sorbate. 14. A method of preventing clogging of enteral feeding tubes by inhibiting the growth of microorganisms in enteral feeding tube compositions comprising adding to said enteral feeding tube compositions an alkyl paraben, wherein the alkyl group has 1 to 10m carbon atoms. 15. A method of prolonging the useful life of an enteral feeding tube comprising adding to an enteral feeding tube compositions an alkyl paraben, wherein the alkyl group has 1 to 10 carbon atoms.

Documents:

514-mas-2000-abstract.pdf

514-mas-2000-assignement.pdf

514-mas-2000-claims filed.pdf

514-mas-2000-claims granted.pdf

514-mas-2000-correspondnece-others.pdf

514-mas-2000-correspondnece-po.pdf

514-mas-2000-description(complete) filed.pdf

514-mas-2000-description(complete) granted.pdf

514-mas-2000-form 1.pdf

514-mas-2000-form 26.pdf

514-mas-2000-form 3.pdf

514-mas-2000-form 5.pdf

514-mas-2000-other documents.pdf