Title of Invention

"A METHOD OF MAKING SUBSTITUTED PEPTIDES OR SALT THEREOF WITH ANTIDEPRESSANT ACTIVITY".

Abstract

The present invention discloses a method of making substituted peptides or salt thereof with antidepressant activity having a general formula (5) : R1-AA1-R2-Pro3-AA2-AA4-AA5-Gly-AA3-R (5) where Pro1 is the amino acid Pro or dehydro-Pro; AA1 is the amino acid Phe or Tyr; AA2 is selected from the group consisting of Leu, lle, Arg, D-Arg, and Trp; AA3 is the amino acid Trp; AA4 and AA5 is the amino acid Gly or lle; R is selected from the group consisting of a carboxyl group, hydroxalkyl group, a carbamyl group, an alkylcarbamyl group, and an alkoxycarbonyl group; and, R1 and R2 each independently are selected from the group consisting of a hydrogen atom, a lower alkyl group having 1 to 3 carbon atoms, a fluorine atom, a chlorine atom, a cis-or trans4-OH-group, a sulphydryl group, and an alkylamino or dialkylammo group which method comprises providing an amide resin, removing Fmoc blocking groups from the amide resin, adding appropriate Fmoc-amino acids, such as herein described, to the resin to form a peptide having a desired amino acid sequence, and cleaving the peptide-resin bond.

Full Text

1 A 1.FIELD OF INVENTION The present invention relates to a method of making substituted peptides or salt thereof with antidepressant activity. This application is divided out of Indian Patent Application No.198/CAL/2 001 ANTEDATED AS OF 01.05.1996. Heterogenous unipolar and bipolar depression is a common psychiatric disorder most likely mediated by neurochemical changes in the central nervous system. Administration of antidepressant drugs for treatment of unipolar depression has gained wide acceptance in the medical community over the past several decades. The present invention discloses novel peptides and their use as therapeutic agents in treating patients suffering from depression. 2. BACKGROUND OF THE INVENTION Endogenous depression is thought to be a genetically determined biochemical disorder which results in an Inability to deal with stress. This form of depression is oftentimes classified as unipolar depression, which is subclassified into retarded depression and agitated depression. Retarded depression is characterized by psychomotor retardation, where the subject does not Interact with the surrounding environment to any extent. Agitated depression is, on the other hand, characterized by increased unproductive activity such as pacing, hand wringing, etc Unipolar depression is most likely a disorder resulting from a number of heterogenous changes in the brain. One school of thought subscribes to the catecholamine theory: 1B that endogenous depression is caused by a reduction in norepinephrine concentration within the vicinity of adrenergic receptor sites in the brain. Another possibility is that endogenous depression is caused by an absolute or relative deficiency in indoleamine, specifically 5-hydroxytryptamine, at receptor sites in the brain. -2- The course of treatment for endogenous depression is electroconvulsive therapy or drug therapy. The drugs administered for therapeutic treatment of depression include (1) tricyclic antidepressants, (2) monoamine oxidase (MAO) inhibitors, and (3) second-generation antidepressants. Tricyclic drugs have been the drug of first choice in treating endogenous depression for over three decades. However, these drugs have limited efficacy in that two-thirds of patients receiving tricyclic drugs do not respond favorably. The side effects of the tricyclics are numerous, including cholinergic blockage, cardiac complications, allergic reactions, dry mouth, constipation, blurred vision and tachycardia. The tricyclic drugs are characterized structurally by a three-ringed nucleus. The tricyclic antidepressants include imipramine, desipramine, amitriptyline, nortriptyline, protriptyline, doxepin and trimipramine. These tricyclic structures are metabolized through the mixed-function oxidase system. These metabolites are the pharmacologically active compounds. The MAO inhibitors have been available for treatment of depression since the 1950's. These compounds are classified either as hydrazides, exemplified by a C-N-N moiety (e.g., phenelzine and isocarboxazid) or a nonhydrazide (e.g., tranylcypromine). These drugs have not gained wide acceptance due to serious side effects. The so called second-generation drugs are a group of new drugs which include amoxapine, maprotiline, fluoxetine, trazodone and bupropion. Most of these drugs seem to act in the same fashion as the tricyclic drugs, Antidepressant drugs must cross the blood-brain barrier in pharmacologically effective concentrations. The capillaries of the central nervous system, unlike capillary beds feeding other organs, possess tight junctions with cerebral endothelial cells. Therefore, the human blood-brain barrier is a lipid barrier without pores. Any potential antidepressant drug must be designed such that the compound is able to traverse the blood-brain barrier. Compounds with low lipid solubility as well as many ionized compounds are unable to exit the circulation for entry into the extracellular fluid of the brain. Water soluble compounds will traverse the blood-brain barrier only if a specific membrane transport system exists. In contrast, lipid soluble drugs, in effect, are not hampered by the blood-brain barrier. -3- The tripeptide MIF, otherwise known as melanocyte stimulating inhibitor,' factor, which is represented by the chemical formula of proiyl-leucyi-glycinamide or Pro-Leu-Gly-NH2, has been shown to produce numerous non-endocrine effects on the brain. The MIF tripeptide has also been shown to be active in a number of animal models for depression. MIF was initially isolated and characterized from bovine hypothalmic extracts (Nair, etal., 1971, Biochem. Biohys. Res. Comm. 43(6): 1376-1381) and rat hypothalmic extracts (Celis, etal., 1971, Proc. Nati. Acad. Sci. USA 68(7): 1428-1433). MIF activity was attributed to inhibiting release of melanocyte stimulating hormone, a pituitary hormone known to stimulate melanin production. Neither disclosure suggests or discloses any potential antidepressant activity for MIF. U.S. Patent No. 3,708,593 (issued to N.P. Plotnikoff on January 2, 1973) teaches that MIF exhibits antidepressant activity in mice, as shown by a modified Dopa test (Everett, et al., 1966, Proc. 1" Int. Sym. Anti-depressant Drugs, p. 164). U.S. Patent No. 3,795,738 (issued to N. P. Plotnikoff on March 5, 1974) teaches that MIF, alone or in combination with other known drugs, exhibits increased activity . against Parkinson's disease. U.S. Patent No. 3,931,184 (issued to C. G. Lex on January 6, 1976) teaches isolation of medicinally pure MIF. A MIF hemihydrate is dissolved in methanol, followed by the addition of diethyl ether, resulting in a white crystalline precipitate of MIF. This pure MIF is collected, washed with ether and dried under vacuum prior to use. U.S. Patent No. 4,278,595 (issued to J. H. Cort on July 14, 1981) teaches that practical use of MIF has been hindered because MIF is rapidly metabolized subsequent to administration. Due to this relatively short half-life of MIF, it has been necessary to administer large quantities of MIF intravenously over prolonged periods to obtain efficacious concentrations. Cort teaches a MIF analog characterized by replacement of Leu with its D-isomer, optionally replacing Pro with pGlu, and optionally alkyiating the terminal amide group of Gly-NH2 to produce a MIF analog. Such an analog may possess similar antidepressant activity as MIF and enhanced stability. Cort teaches a tripeptide having the formula X-D-Ixu-NH2CH2CONR1R2, where X is Pro or pGlu and each of R1 and R2 independently is H or a lower alkyl, especially methyl or ethyl. -4- Tyr-MIF-1 (Tyr-Pro-Leu-Gly-NH3) is a brain derived peptide shown to affect passive avoidance in rats (Hayashi, et al., 1983, Brain Res. Bull. 11: 659-662). Various analogs to Tyr-MIF-1 (i.e., substitutions for the Tyr residue, resulting in Ala-MIF-1, Leu-MIF-1 or Phe-MIF-1) were tested for a possible affect on behavioral and motor activities (Hayashi, et al., 1984, Pharmacology Biochemistry & Behavior 21; 809-812). Ala-MIF-1 and Phe-MIF-1, but not Leu-MIF-1, affected passive avoidance behavior in rats. None of these peptides were shown to affect motor behavior. Kastin, et al. (1984, Pharmacology Biochemistry & Behavior 21: 767-771) discloses that MIF-1 and Tyr-MIF-1 are active as antidepressants. The degree of activity was measured by the water wheel test, a modification of the Porsolt swim test. Kastin, et al. (1985, Pharmacology Biochemistry & Behavior 23: 1045-1049) determined that Tyr-MIF-1 and several Tyr-MIF-1 analogs possess antiopiate activity. Along with Tyr-MIF-1, Phe-MIF-1 was active in inhibiting the analgesic effect of morphine in rats. Banks, et al. (1986, Am. J. Physiol. 251 [Endocrine Metabolism 14]: E477-E482) identifies the carrier-mediated transport system responsible for delivery of Tyr-MIF-1 to the extracellular brain fluid from the circulatory system. It would be extremely useful to design and generate modified small peptides for treating patients suffenng from depression which possess pharmacological activity subsequent to crossing the blood-brain barrier without inducing the side effects inherent in many of approved antidepressant drugs available today. SUMMARY OF THE INVENTION The present invention discloses modified small peptides for use as antidepressant compounds. According to the invention, these novel peptides are utilized to treat patients suffering from depression. These modifications target amino terminus residues, carboxyl terminus residues and internal residues, including addition and substitution of amino acid residues and modification of the peptide bonds and functional side groups of respective amino acid residues as more fully described hereinbelow. It is an object of the invention to provide peptides which have pharmacologic activity. It is another object of the invention to provide peptides useful in treating patients exhibiting symptoms from depression. It is a feature of the invention to synthesize and provide small peptides representing novel modifications, substitutions, additions and/or deletions to a MIF core structure which have anti-depressant activity. It is an advantage of the invention to provide small peptides which may be administered at lower dosages than known anti-depressants so as to reduce potential deleterious side effects. -6- Accordingly, the present invention discloses a method of making substituted peptides or salt thereof, with antidepressant activity having a general formula (5) : R1-AA1-R2-Pro1-AA2-AA4-AA5-Gly-AA3-R (5) where Pro1 is the amino acid Pro or dehydro-Pro; AA1 is the amino acid Phe or Tyr; AA2 is selected from the group consisting of Leu, Ile, Arg, D-Arg, and Trp; AA3 is the amino acid Trp; AA4 and AA5 is , -7- the amino acid Gly or Ile; R represents a caboxyl group, hydroxyalkyl group, a carbamyl group, an alkylcarbamyl group, 1 2 or an alkoxycarbonyl group, and, R and R each independently represent a hydrogen atom, a lower alkyl group, preferably having 1 to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group, preferably a cis-or or trans-4-OH- group, a sulphydryl group, preferably a cis-or or trans-4-thio- group, or an alkylaminc or dialkylamino group, preferably a methyl or ethylamino or dimethyl or diethylamino group, or a phoshphono group (preferably as phosphono-tyrosine). A preferred peptide of formula (5) includes but is not limited1 to 4-F-Phe-4-OH-Pro-Arg-Gly-Ile-Gly-Trp-NH2 (SEQ ID NO:56). -8- -9- hydroxyalkyl - alcohol group or -ROH where R represents a lower alkyl group, preferably having 1 to 3 carbon atoms; carbamyl - 1° amide group or -CONH2; alkyicarbamyl - 2° or 3° alkylated amide group or -CONR1R2 where R1 and R2 each independently represent a hydrogen atom, a lower alkyl group, preferably having 1 to 3 carbon atoms; alkoxycarbonyl - ester group or -CO2R where R represents a lower alkyl group, preferably having 1 to 3 carbon atoms dehydro - anhydro group where one or more hydrogen atoms are removed; hydroxyl - alcohol group or -OH or -ROH where R represents a lower alkyl group, preferably having I to 3 carbon atoms; sulphydryl - thiol group -SH or -RSH where R represents a lower alkyl group, preferably having 1 to 3 carbon atoms; alkylamino - -NHR where R represents a lower alkyl group, preferably having 1 to 3 carbon atoms; dialkylamino - -HR2 where R represents a lower alkyl group, preferably having 1 to 3 carbon atoms; patient - includes any member of the animaJ kingdom, including but not solely limited to humans; and, CGI - Control group inactive. -10- 4. BRIEF DESCRIPTION OF THE ACCOMPANYING/FIGURES There are shown in the Figures certain exemplary small peptide compositions of the invention and pharmacological efficacy thereof as antidepressants as presently preferred. It should be understood that the invention is not limited to the embodiments disclosed as examples in the figures and the accompanying Table 1, and is capable of variation within the scope of the appended claims. In the drawings, FIGURE 1 shows the average number of responding animals in the Porsolt swim test for selected compounds listed 1-15, as follows: 1. 4-F-Phe-3,4-Dehydro-Pro-Arg-Gly-Trp-NH2(SEQ ID NO:55) 2. 4-F-Phe-4-OH-Pro-Arg-Gly-Iie-Gly-TRF-NH3(SEQ ID NO.56) 3. 4-F-Phe-4-Homo-Pro-Arg-Gly-Trp-NH2 (SEQ ID.NO:57) 4. Pro-Ile-Gly-Trp-NH;, (SEQ ID NO:3) 5. 4-F-Phe-4-OH-Pro-Ile-GIy-NH2 (SEQ ID NO: 16) 6. Amitriptyline 7. 4-F-Phe-4-OH-Pro-Arg-Gly-Gly-Trp-NH2(SEQ ID NO:58) 8. 4-CH30-Phe-4-OH-Pro-Arg-Gly-Trp~NH2 (SEQ ID NO:59) 9. 4-Cl-Phe-4-OH-Pro-Ile-Gly-NH2(SEQ ID NO:60) 10. 4-F-Phe-4-OH-Pro-Ile-Gly-Trp-NH2 (SEQ ID NO:41) 11. 4-F-Phe-4-OH-Pro-Arg-Gly-Trp-NH3 (SEQ ID NO:43) 12. 4-OH-Pro-IIe-Gly-NH2 13. Zoloft 14. Prozac, and 15. Pro-Leu-Gly-NH2. Peptides were administered at 0.1 mg/ml in these selected examples; FIGURE 2 shows the mean mobile time for difference between the CGI and selected compounds listed as 1-15, as above for Figure 1; and, FIGURE 3 shows the mean of 2-score of calculated mobile time for selected compounds listed as 1-15, as above for Figure 1. -11- 5. DETAILED DESCRIPTION OF THE INVENTION The tripeptide hormone fragment having the general formula Pro-Leu-Giy-NH2, otherwise known as L-prolyl L-leucyl glycine, melanocyte stimulating inhibitory factor, melanotrophic release inhibiting factor, or MIF, is known to exhibit antidepressant activity. MIF is typically reported in literature as having the tripeptide structure Pro-Leu-GIy-NH2 or Pro-Leu-Gly-amide. MIF will be referred to herein as Pro-Leu-Gly-NH2 having the following chemical structure: According to the invention, modifications of the tripeptide structure of MIF result in novel small peptides utilized to treat patients suffering from depression. These modifications target amino terminus residues, carboxyl terminus residues and internal residues, including addition and substitution of amino acid residues and modification of the peptide bonds and functional side groups of respective amino acid residues as more fully described hereinbelow. In general, the amino acid(s) additions or substitutions at the amino terminus (N-terminus), carboxyl terminus (C-terminus), or internal amino acid residues to the MIF core sequence to synthesize the small peptides of the .invention can be selected from the group of Ala, Arg, D-Arg, Gly. He, Leu, D-Leu, Lys, Orn, Phe, Pro, dehydro-Pro, Sar, Trp, and Tyr or any of the remaining amino acids. The carboxyl terminus modifications of the small peptides of the invention can include optional replacement of the carbarn yl (amide) group at the carboxyl terminus of the MIF core sequence by a carboxyl (acid) group, a hydroxyalkyl (alcohol) group, an aikoxycarbonyl (ester) group, or an -12- alkylcarbamyl (alkyiated amide) group, and the like. The amino terminus and internal modifications of the small peptides of the invention can include optional additions or eliminations on the heterocyclic, aromatic, and other carbon residues of the amino acids with an alkyl group, preferably an alkyl group having 1 to 3 carbon atoms, a dehydro (anhydro) group, a halo group, a hydroxyl group, a sulphydryl group, an alkyJamino group, or a dialkylamino group, and the like. Furthermore, the amino groups of the small peptides of the invention can be alkyiated, preferably with an alkyl group having 1 to 3 carbon atoms. It should be understood by a person of ordinary skill that these additions, substitutions, eliminations, and/or modifications can be carried out by conventional polypeptide synthesis and organic chemistry synthesis techniques. ' This specification details extensive biological data supporting the following premise: small peptides disclosed herein show substantial antidepressant activity as measured in the Porsolt swim test. The Example Section contains comparative data of exemplified peptides of the present invention and known antidepressants amitriptyline, fluoxetine (Prozac) and sertraline (Zoloft) generated in a series of Porsolt swim tests. The groupings of the small peptides of the invention into the formulas described below are provided only as a matter of convention and should not be considered limiting in any manner. In one embodiment of the invention, the small peptides are tripeptides characterized either by optional replacement of the Leu residue of the MIF core sequence with an amino acid selected from the group of Trp, Orn, Lys, Arg, D-Arg, or He; optional replacement of the Pro residue with dehydro-Pro, preferably 3,4-dehydro-Pro; optional modification of the carboxyl terminus amide group with a substituent selected from a carboxyl group, an hydroxyaJkyl group, preferably a hydroxymethyl group, an alkoxycarbonyl group, or an alkyiated carbamyl group; optional modification of the amino terminus heterocyclic group or dehydro-heterocyclic group with a substituent selected from the group of a lower alkyl group, preferably having 1 to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group, preferably a cis- or trans-4~OH- group, a sulphydryl group, preferably a cis- or trans-4-ih\o- group; or an alkylamino group or a dialkylamino group, preferably a methyl or ethylarnmo or a dimethyl or diethylamino group; and/or optional modification of the hydrogen atoms at -13- the nitrogen atoms of the amino acid peptide bonds with a lower alkyl group, preferably having 1 to 3 carbon atoms. This tripeptide composition or a pharmaceutically acceptable salt thereof can be represented by the following formula (1): R1-Prol-AA1-NR2-CH2-R (1) where Pro1 represents the amino acid Pro or dehydro-Pro, preferably 3,4-dehydro Pro; AA1 represents an amino acid of the group of Trp, Orn, Lys, Leu, Arg, D-Arg, or He; R represents a carboxyl group, a hydroxyalkyl group, a carbamyl group, an alkylcarbamyl group, or an alkoxycarbonyl group; Rl represents a hydrogen atom, a lower alkyl group, preferably having 1 to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group, a sulphydryl group, or an alkylamino or dialkylamino group, preferably a methyl or ethylamino group or dimethyl or diethylamino group; and, R2 represents a hydrogen ;atom or a lower alkyl group, preferably having 1 to 3 carbon atoms, with the proviso that where Pro1 is Pro and AA1 is Leu, then R1 and R2 cannot both be hydrogen when R is a carbamyl (amide) group, since MIF is a known compound. The following paragraphs discloses compositions of the tripeptides of formula (1), which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression. A first group of preferred compositions of the tripeptides of formula (1) which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression are characterized by replacement of Leu, and are further characterized by having the N-terminus Pro1 residue and C-terminus amide group remain unmodified, which can be represented by formula (la). Formula (la) is depicted as: Pro1-AA1-Gly-NH2; (la) wherein Pro1 and AA1 are as described above for formula (1). The tripeptides of formula (la), may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression. Preferred compositions of the tripeptides of formula (la) are: Pro-Trp-GIy-NH2; Pro-Arg-Gly-NH2; Pro-D-Arg-Gly-NH,; -14- Pro-Lys-GIy-NH2; Pro-Grn-GIy-NH2; and, Pro-Ile-GIy-NH2. A second group of preferred compositions of the tripeptides of formula (1) which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression are characterized by optional replacement of Leu, and are further characterized by optional modification of the N~terminus heterocyclic nitrogen ring of Pro1, preferably at the C-4 position of the heterocyclic nitrogen ring, preferably by addition of a cis- or trans-hy droxyl group or a cis- or /ro/u-sulphydryl group, and by having the Oterminus amide group remain unmodified, which can be represented by formula (lb). Formula (Ib) is depicted as: R1-Pro1-AA1-Gly-NR2 (lb) wherein Pro1, AA1 and R1 are as described above for formula (1). Preferred compositions of the tripeptides of formula (lb) are: cis- or trans-4-OH-Pro-D-Arg-Gly-NH2; cis- or trans-4-OH-Pro-Ile-Gly-NH2; cis- or trans-4-OH-Pro-Arg-Gly-NH2; cis- or trans-4-OH-Pro~Trp-Gly-NH2; and, cis- or trans-4-thio-Pro-Leu-GIy-NH3. A third group of preferred compositions of the tripeptides of formula (1) which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression are characterized by optional replacement of Leu, optional modification of the C-terminus amide group, optional modification of the C-terminus hydrogen atom at the nitrogen comprising the peptide bond between Leu-GIy, and by having the N-terminus heterocyclic nitrogen ring of Pro' remain unmodified, which can be represented by formula (lc). Formula (lc) is depicted as: Pro1-AA1-NR2-CH2-R (lc) wherein Pro1, AA1, and R and R2 are as described above for formula (1), with the proviso that where Pro1 is Pro and AA1 is Leu, R2 cannot be hydrogen when R is either a carboxyl group or a hydroxyalkyl group, since the compounds of Pro-Leu-NHCH3-CO2H -15- (or Pro-Leu-Gly) and Pro-Leu-NHCH2-CH2OH, do not form part of this invention, and with the further proviso that where Pro1 is Pro and AA1 is Trp, R2 cannot be a hydrogen atom when R is a hydroxyalkyl group, since Pro-Trp-NHCHrCH2OH is a known compound. Preferred compositions of the tripeptides of formula (1c) are: Pro-Leu-N(CH3)CH2CONH2; and, Pro-Trp-NHCHrCO2H. In another embodiment of the invention, additional tripeptides are characterized by replacement of Leu with Arg or D-Arg; replacement of Gly with Ala; optional replacement of Pro with dehydro-Pro, preferably 3,4-dehydro-Pro; optional modification of the C-terminus amide group with a functional group selected from the group of a carboxyl group, a hydroxyalkyl group, preferably a hydroxymethyl group, an alkoxycarbonyl group, or an alkylated carbamyl group; optional modification of the N-tenrinus heterocyclic nitrogen ring of Pro1 with a substituent selected from the group of a lower alkyl group, preferably having 1 to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group, preferably a cis- or trans-4~OH- group, a sulphydryl group, preferably a cis~ or trans-A-thio- group; or an alkylamino group or a dialkylamino group, preferably a methyl or ethyl amino or dimethyl or diethyl amino group; and/or optional modification of the hydrogen atoms at the nitrogen atoms of the amino acid peptide bonds with a lower alkyl group, preferably having 1 to 3 carbon atoms. This tripeptlde composition or a pharmaceutically acceptable salt thereof can be represented by the following formula (2): R1-Pro1-AA1-Ala-R (2) where Pro1 represents the amino acid Pro or dehydro-Pro, preferably 3,4-dehydro Pro; AA1 represents an amino acid of the group of Arg or D-Arg; R represents a carboxyl group, a hydroxyalkyl group, a carbamyl group, an alkylcarbamyl group, or an alkoxycarbonyl; and, R1 represents a hydrogen atom, a lower alkyl group, preferably having 1 to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group, a sulphydryl group, or an alkylamino or diaJkyiamino group, preferably a methyl or ethylamino or dimethyl or dielhylamino group. -16- The following paragraph discloses compositions of the tripeptides of formula (2), which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression. A group of preferred compositions of the tripeptides of formula (2) which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression are characterized by replacement of the Leu and Gly, and by having the N-terminus Pro1 residue and C-terminus amide remain unmodified, which can be represented by formula (2a). Formula (2a) is depicted as: Pro1-AA1-Ala-NH2 (2a) wherein Pro1 and A A1 are as described above for formula (2). Preferred compositions of the tripeptides of formula (2a) are: Pro-Arg-Aia-NH2; and, Pro-D-Arg-AIa-NH2. In a further embodiment according to the invention, the small tripeptides are characterized by replacement of Leu with Orn; replacement of Gly with Tyr; optional replacement of Pro with dehydro-Pro, preferably 3,4-dehydro-Pro; optional modification of the C-terminus amide group with a substituent selected from the group of a carboxyl group, a hydroxyalkyl group, preferably hydroxymethyl, an alkoxycarbonyl group, or an alkylated carbamyl group; optional modification of the N-terminus heterocyclic nitrogen ring of Pro1 with a substituent selected from the group of a lower alkyl group, preferably having 1 to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group, preferably a cis- or trans~4-OH- group, a sulphydryl group, preferably a cis- or trans-4-thio- group, or an alkylamino group or a dialkylamino group, preferably a methyl or ethylamino or a dimethyl or diethylamino group; and/or optional modification of the hydrogen,atoms at the nitrogen atoms of the amino acid peptide bonds with a lower alkyl group, preferably having 1 to 3 carbon atoms. This tripeptide composition or pharmaceuticaily acceptable salt thereof can be represented by the following formula (3); R1-Pro1-AA1-Tyr-R (3) where Pro1 represents the ammo acid Pro or dehydro-Pro, preferably 3,4-dehydro Pro; AA1 represents the amino acid Orn; R represents a carboxyi group, a hydroxyalkyl group. -17- a carbamyl group, an alkylcarbarnyl group, or an alkoxycarbonyl group; and, Rl represents a hydrogen atom, a lower alkyl group, preferably having 1 to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group, preferably a cis- or trans-A-OM- group, a sulphydryl group, preferably a cis- or trans-4-thio- group, or an alkylamino or dialkylamino group, preferably a methyl or ethylamino or a dimethyl or diethylamino group. The following paragraphs disclose compositions of the tripeptides of formula (3), which may be utilized alone or in combination with other peptides disclosed in this specification.to treat patients suffering from depression. A group of preferred compositions of the tripeptides of formula (3) which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression are characterized by replacement of Leu and Gly, optional modification of the N-terminus heterocyclic nitrogen ring of Pro1, and by having the C-terminus amide remain unmodified, which can be represented by formula (3a). Formula (3a) is depicted as: Rl-Pro1-AA1-Tyr-NH2 (3a) where Pro1, AA1 and R1 are as described for formula (3). Preferred compositions of the tripeptides of formula (3a) are: Pro-Orn-Tyr-NH2; and, cis- or trans-4-OH-Pro-Orn-Tyr-NH2. -18- In embodiment of the invention the peptides are the peptides are hexapeptide or pharmaceutically acceptable salt thereof including addition of both a N-terminus amino acid and a C-terminus amino acid can be represented by the following formula (4): R1-AA1-R2Pro2-AA2AA4-Gly-AA3R ( 4 ) where Pro1 represents the amino acid Pro or dehydro-Pro; AA1 represents an amino acid of the group of Phe or Tyr; AA2 represents an amino acid of the group of Leu, He, Arg, D-Arg, Trp, or canavanine; AA3 represents the amino acid Trp; AA4 represents the amino acid Gly or lie; R represents a carboxyl group, hydroxyalkyl group, a carbamyl group, an alkylcaxbamyl group, or an alkoxycarbonyl group; and, R1 and R2 each independently represent a hydrogen atom, a lower alkyl group, preferably having I to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group preferably a cis- or trans-4~OH- group, a sulphydryl group, preferably a cis- or irans-4-thio- group, or an aikylamino or dialkylamino group, preferably a methyl or etrrylamino or dimethyl or diethylamino group. A group of preferred hexapeptides of formula (4 ) which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression are characterized by addition of a C-terminus amino acid of Trp, optional modification of the heterocyclic nitrogen ring of Pro1, preferably a cis- or trans-4-OH group, a fluorine atom at position 4 of Phe; preferably Arg at AAZ; Tpr al AA3; and He or Gly at AA4, and by having the C-terminus amide remain unmodified, which can be represented by formula (4a ) Formula (4a) is depicted as: -19- wherein preferred peptides of formula (4 ,a) are: 4-F-Phe-4-OH-Pro-Arg-Gly-Gly-Trp-NH2(SEQ ID NO:58); and 4-F-Phe-4-OH-Pro-Arg-Ile-Gly-Trp-NH2 (SEQ ID NO:67). In another embodiment of the invention, heptapeptides or pharmaceutically acceptable salt thereof including addition of both a N-terminus amino acid and a C-terminus amino acid can be represented by the following formula ( 5); (5 where Pro1 represents the amino acid Pro or dehydro-Pro; AAl represents an amino acid of the group of Phe or Tyr; AAJ represents an amino acid of the group of Leu, Ile, Arg, D-Arg, Trp, or canavanine; AA3 represents the amino acid Trp; A A4 and AA5 represent the amino acid Gly or Ile; R represents a carboxyl group, hydroxyalkyl group, a carbarnyl group, an alkylcarbamyl group, or an alkoxycarbonyl group; and, R1 and R2 each independently represent a hydrogen atom, a lower alkyl group, preferably having 1 to 3 carbon atoms, a halogen atom, preferably a fluorine or chlorine atom, a hydroxyl group, preferably a cis~ or trans-4-OH~ group, a sulphydryl group, preferably a cis- or trans-4-thio- group, or an alkylamino or dialkylamino group, preferably a methyl or ethylamino or dimethyl or diethylamino group, or a phosphono group (preferably as phosphono-Tyr). A preferred p e p t i d e of formula (5 ) is: 4-F-Phe-4-OH-Pro-Arg-Gly-IIe-G]y-Trp-NH2(SEQ ID NO:56). In yet another embodiment of the invention, the peptides are polypeptides including chemical combinations and/or overlapping chemical combinations of any of the small peptides of any of formula (1) through formula (5.) described above which may be utilized alone or in combination with other peptides disclosed in this specification to treat patients suffering from depression. The chemical combinations and/or overlapping chemical combinations of the peptides disclosed preferably range from at least about three to at least about ten amino acids. Examples of such combinations include, but are not necessarily limited to, the compositions as follows: 4-F-Phe-c/j- or /ranj-4-OH-Pro-IIe-Gly-Trp-Gly-NH2 (SEQ ID NO.46), 4-F-Phe-m or //-flnj-4-OH-Pro-Ne-GIy-Trp-GJy-Trp- -20- NH2 (SEQ ID NO:47); 4-F-Phe-cis- or trans-4-OK-Pro-Leu-Gty-Trp-GIy-NH2 (SEQ ID NO:48); 4-F-Phe-cis- or trans-4-OH-Pro-Leu-Gly~Trp~Gly-Trp-NH2 (SEQ ID NO:49); Pro-ne-Gly-Trp-Pro-ne-Gly-KH2; (SEQ ID NO:50) 4-F-Phe-cis- or trans-4-OH-Pro-Arg-Gly-Trp-Gly-NH2 (SEQ ID NO:51); 4-F-Phe-cu- or trans-4-OH-Pro-Arg-Gly-Tip-Gly-Trp-NH2 (SEQ ID NO:52); cis- or trans- 4-OH-Pro-IIe-GIy-cw-or rra/tf-4-OH-Pro-Ile-Gly-NH2 (SEQ ID NO:53); 3,4-dehydro-Pro-D-Arg-Gly-3,4-dehydro-Pro-D-Arg-Gly-NH2; 3,4-dehydro-Pro-D-Arg-Gly-Trp-Gly-NH2; and, 3,4-dehydro-Pro-D-Arg-Gly-Trp-Gly-Trp-NH3. Especially preferred compositions of the present invention encompass small peptides which show increased higher activity in the Porsolt swim test described within this specification. These small peptides may vary in length, with the preferred peptides being tetrapeptides, pentapeptides, hexapeptides and heptapeptides. The formula for these especially preferred peptides, which are disclosed throughout this specification, may be: for a tetrapeptide, wherein R1 is preferably a halogen atom, most preferably a fluorine or. chlorine atom, a carboxyl group, an amino group or a nitro group, with ail modifications preferably at the C4 atom of Phe; Pro1 is 3,4-dehydro Pro, Homo-Pro, cis- or ' trans-AOH-Vro or Pro, as listed in order of preference, AA2 is preferably Ile, Leu or Arg; and AA3 is preferably Gly or Trp. Another preferred tetrapeptide of the present invention is Pro-Ile-Gly-Trp (SEQ ID NO:3). The formula for the especially preferred pentapeptides, hexapeptides and heptapeptides, which are also disclosed throughout this specification, may be: wherein R1 is preferably a halogen atom, preferably a fluorine or chlorine atom, a carboxyl group, an amino group or a nitro group, with all modifications preferably at the C4 atom of Phe; Pro1 is 3,4-debydro Pro, Homo-Pro, cis- or trans-4OH-Pro or Pro, as listed in order of preference, AA2 is preferably Arg, lie, Leu or His, with Arg being especially preferred; AAW is 0-2 amino acid residues, if n=l, then Gly is preferred and if n=2, then Ile-Gly, He-He or Gly-Gly is preferred; A A3 is preferably Trp or Gly, with Trp most preferred. -21- The present invention funher discloses admixtures of the peptides of formula (1) through formula (11) with known antidepressant compounds such as amitriptyline, fluoxetine (Prozac) and sertraline (Zoloft). It is within the ordinary skill of the artisan to generate various admixtures with the small peptides of the present invention beyond the exemplifications disclosed throughout this specification. The small peptides with which this invention is concerned are readily prepared by conventional procedures (i.e., carbodiimide method, mixed anhydride method, N, N-carbonyldiimidazole method) for the step-wise synthesis of polypeptides, and also including solid phase peptide synthesis. The substituents groups are also readily added to the polypeptide residues by conventional procedures. The small peptides of the invention possess antidepressant activity as determined by the Porsolt swim test as described in the Example Section. The Porsolt swim test is based on the observation that when a rat is forced to swim in a situation from which there is no escape, the rat ceases to move altogether and makes only those movements necessary to keep its head above water. Immobility indicates a state of despair. Therefore, a compound with activity as an antidepressant will delay the onset of immobility. The active ingredient, which may comprise one or more of the peptides disclosed in the present invention, should be formulated in a suitable pharmaceutical carrier for in vivo administration to the patient by any standard method known in the art. Appropriate routes of administration include, but are not limited to, oral (mouth or peroral administration), sublingual, parenteral (e.g., intravenous, intraspinal, intrathecal, intraventricular, epidermal, intracisternal, intracutaneous or intradermal, subcutaneous, or intramuscular), epicutaneous or transdermal, intranasal or rectal as well as inhalation (poly or mircodispersed aerosol). As with other pharmaceutical delivery strategies, the primary dosage form depends on the mode of administration. Oral administration includes, but is not limited to tablets, capsules, solutions, suspensions, gels, powders, elixirs or syrups. Sublingual administration includes but is not limited to tablets or lozenges. Parenteral administration includes but is not limited to solutions and suspensions. Epicutaneous or transdermal application will include, but are not necessarily limited to, ointments, creams, pastes, plasters, powders, aerosols, lotions, transdermal patches, discs and solutions. Intranasal -22- administration of the peptide or peptides of the present invention include, but are not limited to, solutions, sprays, inhalants or ointments. Rectal administration may include, but is not necessarily limited to, solutions, ointments or suppositories. The active ingredient may also be formulated for incorporation into liposomes, microcapsules, polymer or wax-based and controlled release preparations. For additional guidance regarding routes of administration and the generation of pharmaceutically effective dose rates, see Chapter 3 -"Dosage Form Design: Biopharmaceutical Considerations" in Pharmaceutical Dosage Forms and Drug Delivery Systems, 1990, Ansel, H.C. and Popovich, N.G., Fifth Ed.; Lea and Febiger, Philadelphia. The concentration of the peptide(s) used in any of the aforementioned formulations will depend upon the effective dose and the mode of administration used to elicit the appropriate biological effect. The dose should be sufficient to achieve circulating plasma concentrations of the active ingredient such that effective amounts cross the blood-brain barrier that are efficacious. For example, when the tetrapeptide Pro-Leu-GIy-Trp-NH2 is the active ingredient, a circulating plasma level from about 30 mg to about 90 mg per average adult may be used; preferably about 60 mg per average adult. Effective doses for various routes of administration may be extrapolated from dose-response curves derived from in viiro or animal model test systems. The invention will be further clarified by a consideration of the following examples, which are intended to be purely exemplary of the synthesis and use of the small peptides of the invention. 6. EXAMPLE: A TR1- TETRA- OR PENTA- PEPTIDE AS AN ANTIDEPRESSANT 6.1. MATERIALS AND METHODS 6.1.1. PEPTIDE SYNTHESIS Small peptides of the present invention were synthesized by methods known to one of ordinary skill in the art. Briefly, 5mM of Rink amide resin is placed in a reaction vessel of an Applied Biosystem 431A peptide synthesizer. Double couple cycles were used since the amount of starting resin was twice that of a standard run. The following steps were then carried out: (1) the Fmoc blocking group on the resin was first removed by washing with 20% piperidinc; (2) the resin is then washed with N-methyl-pyrrolidinone; (3) 2 mM Fmoc-proline was added to the reaction vessel together with -23- 0,45mM 2-(LH-benzotria2o[-I-yi)-l,I,3,3,-tetramethyiuroniurn hexafiuorophosphate in a solution of 1-hydroxybenzotriazole and imM of diisopropylef-hylamlne; (4) the resin was washed with N-methylpyrrolidinone; (5) the cycle was repeated with Fmoc-Ieucine, then with Fmoc-glycine and finally with Fmoc-tryptophan; (6) the peptide resin bond was cleaved with the following solution: 0.25 ml ethanedithiol, 0.25 ml H2O, and 9.5 ml TFA, with a cleavage time of 3-4 hours; (7) the peptide was then purified by two reverse phase HPLC procedures, the first in 0.1 % TFA in water as the eluting solvent and the second using 0.1% TFA in acetonitrile as the eluting solvent. The purity of synthesized peptides was analyzed using a HP1090L analytical HPLC equipped with a diode ray. A UV trace showing absorption of the peptide from 220 nm to 310 nm was plotted. Molecular weights were verified by mass spectrometry. The purity of synthesized peptides was analyzed using a HP1090L analytical HPLC equipped with a diode ray. A UV trace showing absorption of the peptide from 220 nm to 310 nm was plotted. Molecular weights were verified by mass spectrometry. 6.1.2. SYNTHESIS OF PRQ-ARG-GLY-NH, Pro-Arg-Gly-NH3 was synthesized manually using a fritted funnel with the step sequence given as for synthesis of Pro-Leu-Gly-Trp-NH2 (SEQ ID NO:5) and the appropriate Fmoc-amino acids. Regarding synthesis of Pro-Arg-Gly-NH2, dimethylformamide was used in place of N-methylpyrrolidinone, and in step (6) the peptide resin bond was cleaved using the following solvent mixture: 0.75 g phenol in 0.25 ml thioanisole, 0.5 mi H20 and 10 ml TFA. The other peptides disclosed in this specification, except the tripeptide Pro-Arg-Gly-NH2, were prepared as described in Section 6.1.2. One of ordinary skill in the art could utilize any of a number of known techniques for synthesizing the peptides of the present invention. Each purified peptide was stored frozen as a white crystalline powder. MIF was also synthesized by techniques known to one of ordinary skill in the art. This tripeptide was stored frozen as a white crystalline powder. 6.1.3. VARIATION OF THE PORSOLT SWIM TEST TO IDENTIFY PEPTIDES WITH ANTIDEPRESSANTrLACTIVITY -24- Male Sprague Dawley rats were obtained from Charles River Laboratories, Wilmington, MA. The rats were housed individually in stainless steel one half inch wire mesh cages sized in accordance with the "Guide for the Care and Use of Laboratory Animals" of the Institute of Laboratory Animals Resources National Research and Counsel. The animal rooms were kept under a twelve hour light/twelve hour dark cycle, with temperature maintained at 18° to 26°C and relative humidity at 40% to 70%. The test animals were acclimated for a minimum of seven days prior to initiation of the study. Daily doses of the small peptides of the present invention were prepared by dissolving 1 mg q.s. to 10.0 ml 0.01M acetic acid in 0.9% saline. 1 ml/kg of the respective peptide wasNthen administered by jntraperitoneal injection once a day for five consecutive days. The antidepressant activity of the peptides of the present invention was determined by a variation of the Porsolt swim test (Porsolt, et al., 1977, Nature 266: 730-732). The method is based on the observation that a rat, when forced to swim in a situation from which there is no escape, will, after an initial period of vigorous activity, eventually cease to move altogether and make only those movements necessary to keep its head above water. Immobility indicates a state of despair in which the conditioned rat cannot escape and resigns itself to the experimental situation. On the first day of the study, the animals were plunged individually into a vertical plexiglass cylinder (40 cm in height and 18 cm in diameter) containing 24 cm of water maintained at 25-26° C. The cylinder was painted white. The water was changed between each rat. After 15 minutes in the cylinder the rats were removed and returned to their individual cages without being dried. One hour later the rats were administered the tetrapeptide, MIF or a control (.01M acetic acid in 0.9% saline), based on individual bodytweight. On days 2, 3, and 4, at the time of day that corresponds to the average injection time of the entire study group of animals, the rats were injected with either peptide 1-39 or the control solution. On day 5, after being weighed each individual rat was respectively dosed based on individual body weight. Doses were staggered in such a manner as to provide ample time for test evaluation to be conducted between animals. Fifteen minutes following injection, each rat was placed in the water for 300 seconds. The mobile time for each rat was recorded. -25- 6.2. RESULTS Table 1 and Figures i-3 depict results generated from numerous Porsolt swim tests acjministered with peptides of the present invention, known antidepressant compounds (e.g., amitriptyline, fluoxetine [Prozac], and sertraline [Zoioft]), known compounds (e.g., Pro-Leu-Gly~NH2, Pro-D-Leu-Gly-NH2 ), and a placebo (CGI: 0.1M acetic acid in 0.9% saline at 1 ml/kg). The number of responding animals out of a possible 12 animals utilized in each test group is reported in column 4 of Table 1 for all compounds, doses and combinations tested. Figure 1 provides a graphical analysis of the average number of responding animals out of a possible 12 animals compiled in one or more experimental groups for selected compounds of the present invention, as well as test compounds. More specifically, the data for the following compounds is listed in Figure 1 as follows: 1. 4-F-Phe-3,4-Dehydro-Pro-Arg-GIy-Trp-NH2 (SEQ ID NO:55), corresponding to listing #2 of Table 1; 2. 4-F-Phe-4-OH-Pro-Arg-Gly-Ile-Gly-TRP-NH2(SEQ ID NO:56), corresponding to listing #3 of Table 1; 3. 4-F-Phe-4-Homo-Pro-Arg-Gly~Trp-NH2 (SEQ ID NO: 57), corresponding to listing #4 of Table 1; 4. Pro-Ile-Gly-Trp-NH2 (SEQ ID NO:3), corresponding to listing #5 of Table 1; 5. 4-F-Phe-4-OH-Pro-Ile-Gly-NH2 (SEQ ID NO: 16), corresponding to listing #8 of Table 1; 6. Amitriptyline, corresponding to listing #9 of Table 1; 7. 4-F-Phe-4-OH-Pro-Arg-Gly-Gly-Trp-NH;, (SEQ ID NO:58), corresponding to listing #11 of Table 1; 8. 4-CH30-Phe-4-OH-Pro-Arg-Gly-Trp-NH2 (SEQ ID NO:59). corresponding to listing #12 of Table 1; 9. 4-Cl-Phe-4-OH-Pro-IIe-GIy-NH2 (SEQ ID NO:60), corresponding to listing #13 of Table 1; 10. 4-F-Phe-4-OH-Pro-Ile-Gly-Trp-NH2 (SEQ ID NO:41), corresponding to listing #14 of Table 1; -26- 11. 4-F-Phe-4-OH-Pro-Arg-Gly-Trp-NH,(SEQ ID NO:43), corresponding to listing #17 of Table I; 12. 4-OH-Pro-Ile-Gly-NH2, corresponding to listing #20 of Table 1; 13. Zoloft, corresponding to listing #41 of Table 1; 14. Prozac, corresponding to listing #48 of Table 1; and 15. Pro-Leu-Gly-NH2. corresponding to listing #55 of Table 1. Compounds #1-15 listed above also correspond to the graphical data presented in Figure 2 and Figure 3. Compound designations are for reference purposes only. The numerical designations utilized in Table 1 do not correspond to data entries as Compound #1-15 in Figure 1, Figure 2 and Figure 3. The data Reported in Figures 1-3 correspond to tests using the standard 0.1 mg/ml dosage. Data presented in Table 1 lists this data as well as numerous additional tests with other peptides of the present invention, various . combinations of compounds and at varying dosages. A responding animal is defined as an animal whose mobile time during the 300 second exposure to the water tank was of longer duration than the average mobile time of the CGI group plus one standard deviation. For example, if the average mobile time for CGI group of twelve treated rats is 35 seconds plus or minus one standard deviation of 15 seconds, then a responding animal is defined as an animal exhibiting a mobile time greater than fifty seconds. Therefore, the control group mean plus one standard deviation was talcen as a threshold for response; the number of rats with mobile times exceeding this level is tabulated as a responding animal. Table 1 (column 5) gives the difference between the mobile times of the various compounds, combination of compounds and dosages tested and the mobile time of CGI. The average of mean mobile time for each peptide are presented. The average mean mobile time between experimental groups for each compound listed above as Compound #1 - 15, represented by a double asterisk, is also presented as pan of Figure 2. Figure 2 illustrates the mean of mobile time for peptides identified above as -27- compounds #1-15. The treatment means were compared with control (CGI) means by a one-tailed Student's t-test. The probabilities so obtained are given in Table 1 (column 8) for the all compounds tested. The Z-score of for mean of mobile time for Compound #1 -15, again as listed above, is reported in Figure 3 and Table 1 (at column 10). The Z-score is a dimensionless measure of efficacy that was averaged over the numerous studies. The mean of Z-score is defined as the difference between the treatment group's mean mobile time and the control group's mean mobile time, divided by the standard deviation of the mean mobile time of the control group. Therefore, an inefficacious compound will generate a Z-score near zero, and higher Z-scores denote increasing efficacy. Data presented in Table 1 and the graphic representation of data listed above as compounds #1-15 in Figures 1-3 indicate that numerous peptides of the present invention exhibit antidepressant-activity in comparison to known antidepressant'compounds such as amitriptyline, Prozac and Zoloft. Additionally, the vast majority of the exemplified peptides of the present invention show greater activity than MIF. The present data also indicates that a synergistic effect may exist between peptides of the present invention and known antidepressant compounds, such as Prozac. For example, data presented in Table 1 at No. I indicate that an admixture of 4-F-Phe-4-OH-Pro-Arg-GIy-Trp-NH2 (SEQ ID NO:43) at 0.1 mg/ml and prozac at 0.8 mg/ml results in 12 of 12 responders. Additionally, data presented in Table 1 at No. 6 indicate that an admixture of 4-F-Phe-4-OH-Pro-Arg-Gly-Trp-NH2 (SEQ ID NO:43) at 0.1 mg/ml and amitriptyline results in 10 of 12 responders. Therefore, Applicants disclose a number of small peptides for use as antidepressant compounds, with biological data to support such use. Other embodiments of the invention will be apparent to those persons skilled in the art from a consideration of this specification or practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with the true scope and spirit of the invention to which exclusive rights are claimed being assessed by the appended claims. n o t ! CO 00 'omments; Tests were run on !2 rats per group and unless otherwise stated, doses are 0.1 mg/kg. CGI: Control group inactive Mobile time = seconds struggling Outliers: Mobile time is over A standard deviation Number of Responders: Number of rats in the Drug Group with Mobile Time > (Mean plus Standard Deviation of Control Group) Difference between Drug & CGI = (Mobile Time of Drug Group minus Control Mobile Time), in seconds Student's t - Test - statistical procedure to determine significant difference between the means of two independent samples (Drug & CGI) Z-Score = (Mobile Time of Drug Group minus Control Mobile Time)/(ControI Standard Deviation) * - significant difference for one-tailed t-Test ** - Average of mean Mobile Time (Drug vs CGI) *** - Average of mean Z-Score (Drug vs CGI) **** - Average of Number of Responders -40-SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANTS: Abajian, Henry B. Noble, John F. Hlavka, Joseph J. (ii) TITLE OF INVENTION: TRI-, TETRA-, PENTA-, AND POLYPEPTIDES AND THEIR THERAPEUTIC USE AS AN ANTIDEPRESSANT AGENT (iii) NUMBER OF SEQUENCES: 73 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Lewis F. Gould, Jr. (B) STREET: 1700 Market Street, Suite 3232 (C) CITY: Philadelphia (D) STATE: PA (E) COUNTRY: US (F) ZIP: 19103 -(v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS' (D) SOFTWARE: Patentln Release #1.0: Version #1.25/ (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Gould, Jr., Lewis F. (B) REGISTRATION NUMBER: 25,057 (C) REFERENCE/DOCKET NUMBER: 3297-5 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (215) 575-6020 (B) TELEFAX: (215) 575-6015 -41- (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label- 4Hyp /note= "Amino acid #1 is either cis- or trans- 4Hyp" (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:I: (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #1 is either cis- or trans- 4Hyp." -42- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Trp-NH2 /note- "Amino acid #4 is a modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2; (2) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear Xii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label- Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide -43- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 3-4DeH-Pro /note= "Proline residue with a C3 = C4 double bond." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Tip residue: an amide group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: -44- (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LBNGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Tyr-NH2 /note = "A modified Tyr residue: an amine group, replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy.-terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: -45- (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label- Trp-NH2 /note= "A modified Tip residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Tyr-NH2 /note^ "A modified Tyr residue: an amine group replaces a hyrdoxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: -46- (2) INFORMATION FOR SEQ ID NO: 10; (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Gly-NH3 /note= "A modified Gly residue: an amine group replaces a hyrdoxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: Trp Pro Leu Xaa 1 (2) INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label- Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: -47- (2) INFORMATION FOR SEQ ID NO: 12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note= The residue modified at C4 with a fluorine atom." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label- Gly-NH2 /note = "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12: Xaa Pro Leu Xaa 1 (2) INFORMATION FOR SEQ ID NO: 13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4C1-Phe /note= "Phe residue modified at C4 with a chlorine atom." -48- (ix) FEATURE: (A) NAME/KEY: Mcdified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label= GIy-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyi group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13: (2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: I (D) OTHER INFORMATION: /label- 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom" (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label- Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyi group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14: -49- (2) INFORMATION FOR SEQ ID NO: 15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 arnino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label- 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp" /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Gly-NH2 /note = "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15: (2) INFORMATION FOR SEQ ID NO: 16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide -50- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note = "Phe residue modified at C4 with a fluorine atom." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note = "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Gly-NH2 /note = "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16: (2) INFORMATION FOR SEQ ID NO: 17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." -51-(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17: (2) INFORMATION FOR SEQ ID NO: 18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18: (2) INFORMATION FOR SEQ ID NO: 19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid .. (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label- 4Hyp /note- "Amino acid #2 is either cis- or trans- 4Hyp. -52- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: (2) INFORMATION FOR SEQ ID NO:20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note = "Amino acid #2 is either cis- or trans- 4Hyp. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = GIy-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20: -53- (2) INFORMATION FOR SEQ ID NO:21: (i) SEQUENCE CHARACTERISTICS: (A)-LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNE5S: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note= "Phe modified at C4 with a fluorine atom.." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus," (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21: (2) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDED NESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4C1-Phe /note= "Phe residue modified at C4 with a chlorine atom. -54- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ 10 NO:22: (2) INFORMATION FOR SEQ ID NO:23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23: (2) INFORMATION FOR SEQ ID NO:24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: pepdde -55- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = GIy-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24: (2) INFORMATION FOR SEQ ID NO:25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 3 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #3 is either cis- or trans- 4Hyp" (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25: -56- (2) INFORMATION FOR SEQ ID NO:26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 3 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #3 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26: (2) INFORMATION FOR SEQ ID NO: 27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Gly-NH3 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." -57- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27: (2) INFORMATION FOR SEQ ID NO:28: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label- GIy-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the caiboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28: (2) INFORMATION FOR SEQ ID NO:29: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 3 (D) OTHER INFORMATION: /label = 4Hyp /note- "Amino acid #3 is eitherxis- or trans- 4Hyp." -58 (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29: (2) INFORMATION FOR SEQ ID NO:30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 3 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #3 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label- Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30: -59- (2) INFORMATION FOR SEQ ID NO:3i: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION; /label = Trp-NH2 /note = "A modified Trp residue: an amine group, replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3I: (2) INFORMATION FOR SEQ ID NO:32: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note="A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32: -60- (2) INFORMATION FOR SEQ ID NO:33: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33: (2) INFORMATION FOR SEQ ID NO:34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hyrdoxyl group at the carboxy terminus." -61-(xi) SEQUENCE DESCRIPTION: SEQ ID NO:34: (2) INFORMATION FOR SEQ ID NO:35: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /labels 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp. (ix) FEATURE: ,(A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note = "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35: (2) INFORMATION FOR SEQ ID NO:36: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide -62- (ix) FEATURE: (A) NAME/KJEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label- 4Hyp /note = "Amino acid #2 is either cis- or trans- 4Hyp. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note = "A modified Tip residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36: (2) INFORMATION FOR SEQ ID NO:37: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /labels Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37: L -63- (2) INFORMATION FOR SEQ ID NO:38: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38: (2) INFORMATION FOR SEQ ID NO:39: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION; 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note- "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." -64- (xi) SEQUENCE DESCRIPTION: SEQ ID WO:39: (2) INFORMATION FOR SEQ ID NO:40: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /labels 4Hyp /note= "Amino Acid #2 is either cis- or trans- 4Hyp" (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 . /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the caxboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40: (2) INFORMATION FOR SEQ ID NO:41: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide -65- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label- 4F-Phe /note = "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label- Trp-NH2 /note- "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41: (2) INFORMATION FOR SEQ ID NO:42: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label- 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp" -66- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42: (2) INTORMATION FOR SEQ ID NO:43: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom, (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label= 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (B) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." -67- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43: (2) INFORMATION FOR SEQ ID NO:44: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44: (2) INFORMATION FOR SEQ ID NO:45: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 4Hyp /note= "Amino acid #1 is either cis- or trans- 4Hyp. -68- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:45: (2) INFORMATION FOR SEQ ID NO:46:. (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 6 (D) OTHER INFORMATION: /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." -69- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46: (2) INFORMATION FOR SEQ ID NO:47: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note = "Phe residue modified at C4 with a fluorine atom, (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label- 4Hyp /note = "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 7 (D) OTHER INFORMATION: /label = Trp-NH3 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47: -70 (2) INFORMATION FOR SEQ ID NO:48: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: amino acid (C)STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 CD) OTHER INFORMATION: /label- 4F-Phe /note- "Phe residue modified at C4 with a fluorine atom." (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 6 (D) OTHER INFORMATION: /label= Gly-NH2 /note- "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4S: (2) INFORMATION FOR SEQ ID NO:49: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide -71- (ix) FEATURE: (A) NAME/KEY: Modified-site . (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 7 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:49: (2) INFORMATION FOR SEQ ID NO:50: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME7KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label= Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:50: -72- (2) INFORMATION FOR SEQ ID NO:51: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label- 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label= 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 6 (D) OTHER INFORMATION: /label = Gly-NH3 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:51: (2) INFORMATION FOR SEQ ID NO:52: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide -73- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note- "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 7 (D) OTHER INFORMATION: /label = Trp-NH2 /note- "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: 3EQ ID NO:52: Xaa Xaa Arg Gly Trp Gly Xaa 1 5 (2) INFORMATION FOR SEQ ID NO:53: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (li) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 4Hyp . /note= "Amino acid #\ is either cis- or trans- 4Hyp. (ix) FEATURE: (A) NAME/KEY: Modified-site (B)LOCATION: 4 (D) OTHER INFORMATION: /label= 4Hyp /note= "Amino acid #4 is either cis- or trans- 4Hyp. 74- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 6 (D) OTHER INFORMATION; /label = Gly-NH2 /note= "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:53: (2) INFORMATION FOR SEQ ID NO:54: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (IX) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Gly-NH2; /note= "A modified Gly residue: an amine group replaces a hyrdoxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:54 (2) INFORMATION FOR SEQ ID NO:55: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDED NESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide -75- (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label=3-4DeH-Pro /note= "Proiine residue with a C3=C4 double bond." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group' replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:55: (2) INFORMATION FOR SEQ ID NO:56: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label- 4Hyp /note= "Ammo acid #2 is either cis- or trans- 4Hyp." -76- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 7 (D) OTHER INFORMATION: /label = Trp-NH2 /note = "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:56: (2) INFORMATION FOR SEQ ID NO:57; (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note- "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = Homo-Pro /note= "Amino acid #2 is Homo-Pro." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label- Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." -77- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:57: (2) INFORMATION FOR SEQ ID NO:58: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /labels 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom, (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp."- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 6 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:58: (2) INFORMATION FOR SEQ ID NO:59: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 ammo acids (B) TYPE: amino acid -78- (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 4CH3O-Phe /note= "Phe residue modified at C4 with a carboxyl group. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label= 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:59: (2) INFORMATION FOR SEQ ID NO:60: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 CD) OTHER INFORMATION: /label= 4C1-Phe /note= "Phe residue modified at C4 with a chlorine atom. -79- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label= 4Hyp /note = "Amino acid #2 is either cis- or trans- 4Hyp. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Gly-NH2 /note = "A modified Gly residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:60: (2) INFORMATION FOR SEQ ID NO:61: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 CD) OTHER INFORMATION: /label = 4C1-Phe /note- "Phe residue modified at C4 with a chlorine atom, (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label= 4Hyp /note = "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 CD) OTHER INFORMATION: /label= Trp-NH? /note= "A modified Trp residue: an amine group -80- replaces a hydroxyl group at ihe carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:61: (2) INFORMATION FOR SEQ ID NO:62: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 3-4DeH-Pro /note = "Proline residue with a C3 = C4 double bond. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 4 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:62: (2) INFORMATION FOR SEQ ID NO:63: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear -81- (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4-NH2-Phe /note= "Phe residue modified at C4 with an amine group." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note = "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:63: (2) INFORMATION FOR SEQ ID NO:64: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note = "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site -82- (B) LOCATION: 2 (D) OTHER INFORMATION: /label= 4Hyp /note= "Ammo acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:64: (2) INFORMATION FOR SEQ ID NO:65: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: iineax (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 4-NO2-Phe /note= "Phe residue modified at C4 with a nitro group. (ix) FEATURE: (A) NAME/KEY: Modifier-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label- 4Hyp /note="Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus -83- (xi) SEQUENCE DESCRIPTION: SEQ ID NO;65: (2) INFORMATION FOR SEQ ID NO:66: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 3F-Phe /note= "Phe residue modified at C3 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:66: (2) INFORMATION FOR SEQ ID NO:67: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: amino acid -84- (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label= 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note = "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 6 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:67: (2) INFORMATION FOR SEQ ID NO:68: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 2F-Phe /note= "Phe residue modified at C2 with a fluorine atom, -85- (IX) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:68: (2) INFORMATION FOR SEQ ID NO:69: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label- 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = Homo-Pro /note= "Amino acid #2 is Homo-Pro." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION- /label= Trp-NH3 /note= "A modified Trp residue: an amine group 6- replaces a hydroxyl group at the carboxy terminus. (xi) SEQUENCE DESCRIPTION: SEQ ID NO:69: (2) INFORMATION FOR SEQ ID NO:70: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: ammo acid (C) STRAND ED NESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 3-(3-pyridyl)-Ala /note= "Ala residue modified at branch methyl group with a pyridyl group." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:70: -87 (2) INFORMATION FOR SEQ ID NO:71: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: I (D) OTHER INFORMATION: /label = 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label- 4Hyp /note= "Amino acid #2 is either cis- or trans- 4Hyp." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 3 (D) OTHER INFORMATION: /label- Homo-Arg /note= "Amino acid #3 is Homo-Arg." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:71: (2) INFORMATION FOR SEQ ID NO:72: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid -88- (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /label = 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label = 3-4DeH-Pro /note= "Proline residue with a C3 = C4 double bond." (ix) FEATURE: (A) NAME/KEY: Modified-sire (B) LOCATION: 5 (D) OTHER INFORMATION: /label= Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:72: (2) INFORMATION FOR SEQ ID NO:73: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION: 1 (D) OTHER INFORMATION: /labels 4F-Phe /note= "Phe residue modified at C4 with a fluorine atom -89- (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /label= 4Hyp /note= "Ammo acid #2 is either cis- or trans- 4Hyp. (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 3 (D) OTHER INFORMATION: /label = L-Allo-Ile /note- "Amino acid #3 is L-Allo-Ile." (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 5 (D) OTHER INFORMATION: /label = Trp-NH2 /note= "A modified Trp residue: an amine group replaces a hydroxyl group at the carboxy terminus." (xi) SEQUENCE DESCRIPTION: SEQ ID NO:73: -90- WE CLAIM: 1. A method of making substituted peptides or salt thereof, with antidepressant activity having a general formula (5) : where Pro1 is the amino acid Pro or dehydro-Pro; AA1 is the amino acid Phe or Tyr; AA2 is selected from the group consisting of Leu, lle, Arg, D-Arg, and Trp; AA3 is the amino acid Trp; AA4 and AA5 is the amino acid Gly or He; R is selected from the group consisting of a carboxyl group, hydroxalkyl group, a carbamyl group, an alkylcarbamyl group, and an alkoxycarbonyl group; and, R1 and R2 each independently are selected from the group consisting of a hydrogen atom, a lower alkyl group having 1 to 3 carbon atoms, a fluorine atom, a chlorine atom, a cis-or trans4-OH-group, a sulphydryl group, and an alkylamino or dialkylamino group which method comprises providing an amide resin, removing Fmoc blocking groups from the amide resin, adding appropriate Fmoc-amino acids, such as herein described, to the resin to form a peptide having a desired amino acid sequence, and cleaving the peptide-resin bond. 2. A method of making substituted peptides substantially as herein described, particularly with reference to the examples and the accompanying drawings. The present invention discloses a method of making substituted peptides or salt thereof with antidepressant activity having a general formula (5) : R1-AA1-R2-Pro3-AA2-AA4-AA5-Gly-AA3-R (5) where Pro1 is the amino acid Pro or dehydro-Pro; AA1 is the amino acid Phe or Tyr; AA2 is selected from the group consisting of Leu, lle, Arg, D-Arg, and Trp; AA3 is the amino acid Trp; AA4 and AA5 is the amino acid Gly or lle; R is selected from the group consisting of a carboxyl group, hydroxalkyl group, a carbamyl group, an alkylcarbamyl group, and an alkoxycarbonyl group; and, R1 and R2 each independently are selected from the group consisting of a hydrogen atom, a lower alkyl group having 1 to 3 carbon atoms, a fluorine atom, a chlorine atom, a cis-or trans4-OH-group, a sulphydryl group, and an alkylamino or dialkylammo group which method comprises providing an amide resin, removing Fmoc blocking groups from the amide resin, adding appropriate Fmoc-amino acids, such as herein described, to the resin to form a peptide having a desired amino acid sequence, and cleaving the peptide-resin bond.

Documents:

00483-cal-2002 abstract.pdf

00483-cal-2002 assignment.pdf

00483-cal-2002 claims.pdf

00483-cal-2002 correspondence.pdf

00483-cal-2002 description(complete).pdf

00483-cal-2002 drawings.pdf

00483-cal-2002 form-1.pdf

00483-cal-2002 form-18.pdf

00483-cal-2002 form-2.pdf

00483-cal-2002 form-3.pdf

00483-cal-2002 form-5.pdf

00483-cal-2002 letters patent.pdf

00483-cal-2002 p.a.pdf

483-CAL-2002-FORM 15.pdf