Title of Invention

ORGANO-PHOSPHOROTJS COMPOUNDS FOR ACTIVATING GAMMA/DELTAT CELLS

Abstract

A method of preparing gamma/delta T-cell activating compounds having formula as depicted in any one of formula (I), (IIA), (IIB), (IIC) and (III) characterized in that, in Corynebacterium ammoniagenes, deleting the LytB gene, inactivating or changing in known manner, enzymatic activity of the gene product, and thereby enriching the compounds by isolating from cells of Corynebacterium ammoniagenes wherein digesting the cell mass in 50 mM ammonium formate buffer (pH 8.0) with Dynax mill; eluting digested material onto an anion exchanger with stepped gradient (100, 300, 500 mM ammonium formate buffer, pH 8.0) after preabsorption on a hydrophobic polystyrene matrix; ultra-filtering the elute of 300mM by passing through a C-18 matrix and through a 3 Kda hallow fibre filter; further eluting the filtrate after ultra-filtration on anion exchanger by diluting with water to 30 mM ammonium formate and precipitating barium salt from the said eluted fraction by admixing 100 mM BaCh and 80% ethanol. Dated this on 13th day of January 2004

Full Text

FORM 2 THE PATENTS ACT, 1970 (39 of 1970) COMPLETE SPECIFICATION (See Section 10, rule 13) METHOD OF PREPARING GAMMA/T-CELL ACTIVATING COMPOUNG BIOAGENCY AG of SCHNACKENBURGALLEE 116a, 22525 HAMBURG, GERMANY, GERMAN Company The following specification particularly describes the nature of the invention and the manner in which it is to be performed : - GRANTED 1-3-2005 Organophosphorus compounds for the activation of ganunn/delta T-cells Numerous diseases in humans and animals arc caused by the abnormal functioning of the immune system. Consequently, there is a high demand for substances that are able to regulate the immune system. ; It is known how to employ the classical acetate/mevalonate pathway in the biosynthesis of isoprenoids (Beycia ED, Porter JW, Annu Rev Biochem. 1976;45:113-42), and an alternative method of biosynthesis is known that is independent of mevalonate, namely the 2-methyl-D-erythritol pathway (MEP, synonymous with DOXP) (Rohmer M. Nat Prod Rep. 1999 Oct;16(5):565-74). Both pathways lead to isopentenyl pyrophosphate (IPP), the common precursor of all higher isoprenoids. While the acctatc/mcvalonatc pathway has been known for a long time and has been thoroughly explained, not all biosynthetic reaction steps that occur along the MEP are as yet known. It is known that human gamma/delta T-cclls are activated by one or several intermediates along the MEP. This means that a selective proliferation and cytokine secretion of the gamma/delta l'-ccll population is brought about during the incubation of peripheral blood lymphocytes with extracts from organisms that possess the MEP (Jomaa H, Feurlc J, Luhs K, Kunzmann V, Tony MP, Herder ich M, Wilhelm M, FEMS Immunol Med Microbiol, 1999 Sep;25(4):371-8). The exact chemical composition of this/these activating substance(s) is still unknown. The published data indicate that 3-formyl-l-bulyl pyrophosphate is formed as a hypothetical intermediate of the MEP and that it plays a role in the activation of gamma/delta T-cells (Belmant C, Espinosa E, Poupot R, Peyrat MA, Guiraud M, Poquet Y, Bonneville M, Fournie J J, J Biol., Chem. 1999 Nov 5;274(45):32079-84). The object of this invention is to provide substances which are able to stimulate gamma/delta T-cells and thereby have a regulatory effect on the immune system. This object is achieved by medicines which contain one or more of the substances defined in Claim 1 as well as in the subordinate claims. Surprisingly, it has been found that compounds of formula (1) are eminently suitable for the activation of gamma/delta T-cclls. wherein R1 is selected from the group comprising a methyl residue, a formyl residue, substituted and unsubstituted hydroxymethyl residues and C0H2R31, wherein R31 is selected from the group comprising OH, substituted and unsubstituted phosphate and substituted and unsubstituted pyrophosphate and R31 and R2 cannot be present in the molecule at the same time, R33 is selected from the group comprising hydrogen, OH, substituted and unsubstituted phosphate and substituted and unsubstituted pyrophosphate, R3 is selected from the group comprising hydrogen, substituted and unsubstituted alkyl with 1 to 26 carbon atoms, substituted and unsubstituted hydroxyalkyl with 1 to 26 carbon atoms, substituted and unsubstituted aryl, substituted and unsubstituted aralkyl, substituted and unsubstituted alkenyl with 1 to 26 carbon atoms, substituted and unsubstituted alkinyl with 1 to 26 carbon atoms, substituted and unsubstituted cycloalkyl, substituted and unsubstituted heterocyclic residues, substituted and unsubstituted phosphate, a silyl, a nucleoside, a nucleoside mono-, di- or triphosphate, a deoxynucleoside, a cation of an organic or inorganic base, particularly a metal of the first, second or third main group of the Periodic Table, ammonium, substituted ammonium and ammonium compounds derived from ethylenediamine or amino acids, and OR34, wherein R34 is defined like R3, X2, inasmuch as a ring is formed between X2 and C1, is defined like X1 and otherwise X2 is selected from the group comprising -OR6, wherein R7 and R8 are defined like R34, wherein R4 is defined like R3, and Z1 is defined like X1, and X3, if it forms a ring with C1, is defined like X1 and, if does not form a ring with C1, corresponds to a group wherein R5 is defined like R3, and Z2 and X4, which forms a ring with C1, are defined like X1, R2 is selected from the group comprising hydrogen, OH, alkoxy, phenoxy, benzyloxy, substituted and unsubstituted phosphate and substituted and unsubstituted pyrophosphate, X1 can be oxygen or wherein Y1 and Y2 can be the same or can be different and arc selected from the group comprising H, OH, halogen, an amino residue, a C1-9-alkoxy residue and a C1-9-alkylthio residue, or together form an oxo group, and a double bond can be present between Co and C1, or between C1 and C2, or between C2 and C3. Preference is given to compounds having the formula: wherein a single or double bond is present between C2 and C3, R1 is selected from the group comprising a methyl residue, a formyl residue and substituted and unsubstituted hydroxymethyl residues, R2 is selected from the group comprising hydrogen, hydroxyl, alkoxy, phenoxy and benzyloxy residues, substituted and unsubstituted phosphate and substituted and unsubstituted pyrophosphate, X1is oxygen or corresponds to a group wherein Y1 and Y2 can be the same or can be different and are selected from the group comprising H, OH, halogen, amino and C1-9-alkoxy and C1-9-alkylthio residues, or together form an oxo group, R3 is selected from the group comprising hydrogen, substituted and unsubstituted alkyl with 1 to 26 carbon atoms, substituted and unsubstituted hydroxyalkyl with 1 to 26 carbon atoms, substituted and unsubstituted aryl, substituted and unsubstituted aralkyl, substituted and unsubstituted alkenyl with 1 to 26 carbon atoms, substituted and unsubstituted alkinyl with 1 to 26 carbon atoms, substituted and unsubstituted cycloalkyl, substituted and unsubstituted heterocyclic residues, substituted and unsubstituted phosphate, a silyl, a nucleoside, a nucleoside mono-, di- or triphosphate, a deoxynucleoside, a cation of an organic or inorganic base, particularly a metal of the first, second or third main group of the Periodic Table, ammonium, substituted ammonium and ammonium compounds derived from ethylenediamine or amino acids, X2, inasmuch as a ring is formed between X2 and C1, is defined like X1, and otherwise X2 corresponds to wherein R4 is defined like R3, and Z1 is defined like X1, and X3, if it fonns a ring with C1, is defined like X1 and, if it does not form a ring with C1, corresponds to a group wherein R5 is defined like R3, and Z2 and X4, which fonns a ring with C1, are defined like X1. Particular preference is given to compounds having formula (HA) wherein C2 and C3 are linked together by either a single or a double bond, R1is a methyl group or a substituted or unsubstituted hydroxymcthyl group, R2 is hydrogen, OH, a substituted or unsubstituted phosphate or a substituted or unsubstituted pyrophosphate, X1 and X2 are selected from the group comprising O, CHF, CHC1, CFC1, CH2, CF2 or CCI2, and R3 is selected from the group comprising hydrogen, substituted and unsubstituted phosphate, a nucleoside, a nucleoside mono-, di- or triphosphate, a deoxynucleoside, a cation of an organic or inorganic base, particularly a metal of the first, second or third main group of the Periodic Table, ammonium, substituted ammonium and ammonium compounds derived from ethylenediamine or amino acids. Preference is also given to compounds having formula (IIB) (IIB) wherein C2 and C3 are linked together by either a single bond or a double bond, R1 is a methyl group or a substituted or unsubstituted hydroxymcthyl group, R2 is H if R1 is a substituted or unsubstituted hydroxymethyl and is OH, a substituted or unsubstituted phosphate or a substituted or unsubstituted pyrophosphate if R1 is a methyl residue, X1, X2 and X3 are selected from the group comprising O, CHF, CHC1, CFC1, CH2, CF2 or CCI2, and R3 and R4 are selected from the group comprising hydrogen, substituted and unsubstituted phosphate, a nucleoside, a nucleoside mono-, di- or triphosphate, a deoxynucleoside, a cation of an organic or inorganic base, particularly a metal of the first, second or third main group of the Periodic Table, ammonium, substituted ammonium and ammonium compounds derived from ethylenediamine or amino acids. Preference is likewise given to compounds having formula (IIC) wherein a single or double bond can be present between C2 and C3, R1is a methyl or a substituted or unsubstituted hydroxymethyl group, R2 is H, OH, a substituted or unsubstituted phosphate or a substituted or unsubstituted pyrophosphate, X1, X2, X3 and X4 are selected from the group comprising O, CHF, CHC1, CFC1, CH2, CF2 or CCI2, and R3, R4 and R5 arc selected from the group comprising hydrogen, substituted or unsubstituted phosphate, a nucleoside, a nucleoside mono-, di- or triphosphate, a deoxynucleoside, a cation of an organic or inorganic base, particularly a metal of the first, second or third main group of the Periodic Table, ammonium, substituted ammonium and ammonium compounds derived from ethylenediamine or amino acids. Moreover, preferred compounds having formulae (II) and (IIA) to (I1C) are those wherein R1 is a substituted or unsubstituted hydroxyinethyl residue, particularly hydroxymethyl itself or a hydroxymethyl residue substituted by phosphate, diphosphate or nucleoside diphosphate, for example a hydroxymethyl residue substituted by uridine diphosphate, and R2 = H. Compounds which are likewise preferred are those having formulae (II) and (IIA) to (IIC) in which R1 is a methyl residue and R2 is a hydroxyl residue, a substituted or unsubstituted phosphate residue or a substituted or unsubstituted diphosphate residue, particularly a nucleoside diphosphate residue, e.g. a uridine diphosphate residue. The following compounds are particularly preferred: wherein the residues R3, R4 and R5 are selected from the group comprising hydrogen, ammonium, sodium or potassium. Furthermore, preferred compounds also include those having the following formula: wherein R31 and R2, which cannot both be present in the molecule at the same time, are selected from the group comprising OH, substituted and unsubstituted phosphate and substituted and unsubstituted pyrophosphate; if R31 is present in the molecule, a double bond is formed between C1 and C2, and a double bond is analogously formed between Co and C1 if R2 is present in the molecule; R33 is selected from the group comprising hydrogen, OH, substituted and unsubstituted phosphate and substituted and xmsubstituted pyrophosphate; R34 is selected from the group comprising hydrogen, substituted or unsubstituted alkyl with 1 to 26 carbon atoms, substituted or unsubstituted hydroxyalkyl with 1 to 26 carbon atoms, substituted or unsubstituted aryl, substituted or unsubstituted aralkyl, substituted or unsubstituted alkenyl with 1 to 26 carbon atoms, substituted or unsubstituted alkinyl with 1, to 26 carbon atoms, substituted or unsubstituted cycloalkyl, a substituted or unsubstituted heterocyclic residue, substituted or unsubstituted phosphate, a silyl, a nucleoside, a deoxynucleoside, a nucleoside mono-, di- or triphosphate, a cation of an organic or inorganic base, particularly a metal of the first, second or third main group of the Periodic Table, ammonium, substituted ammonium or ammonium compounds derived from ethylenediaminc or amino acids; X2 is either -OR6, wherein R6 is defined analogously to R34, or may be wherein R7 and R8 are defined like R34; and X1, X32 and X33 can be the same or can be different and can be oxygen or a group wherein Y1 and Y2 can be the same or can be different and are selected from the group comprising H, OH, halogen, an amino residue, a C1-9-alkoxy residue and a C1-9-alkylthio residue, or together form an oxo group. Particularly preferred compounds are those having formula (11IA) wherein R31 and R2, which cannot be present in the molecule at the same time, are selected from the group comprising OH, substituted and unsubstituted phosphate and substituted and unsubstituted pyrophosphate; if R31 is present in the molecule, a double bond is formed between C1 and C3, and a double bond is formed analogously between Co and C1 if R2 is present in the molecule; R34, R7 and R8 can be the same or can be different and are delined as above; and X1, X32 and X33 can be the same or can be different and are defined as for compound (III). Hydrogen substituents at C1, C2 and C3 are not,explicitly indicated in formulae (I) to (IIIA) for reasons of clarity. However, carbon atoms are understood as being tetravalent. The missing substituents are therefore hydrogen radicals. Furthermore, preference is given to compounds having formula (IIIA) in which R31 and R2 are either OH or substituted or unsubstituted phosphate, R34, R7 and R8 are selected from the group comprising substituted and unsubstituted phosphate, a nucleoside, a deoxynucleoside, a nucleoside mono-, di- or triphosphate, a cation of an organic or inorganic base, particularly a metal of the first, second or third main group of the Periodic Table, ammonium, substituted ammonium and ammonium compounds derived from ethylenediamine or amino acids, and X1, X32 and X33 can be the same or can be different and are O, CHF, CHC1 CFC1, CH2, CF2 or CC12. Other preferred compounds having formula (IIIA) are those in which the phosphate groups are present as sodium, potassium or substituted or unsubstituted ammonium salts. The following compounds arc most suitable: Other embodiments of the invention are defined by the subordinate claims. Peculiarities of the abovementioned definitions and suitable Examples of these will be given below: "Alkyl" is a straight-chain or branched-chain alkyl residue with up to 26 carbon atoms, unless otherwise stated, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tcrt-butyl, pcntyl, hcxyl and the like. Unless otherwise stated, "alkenyl" includes straight-chain or branched-chain alkenyl groups with up to 26 carbon atoms, e.g. vinyl, propcnyl (e.g. I -propenyl, 2-propenyl), 1-methylpropenyl, 2-methylpropenyl, butenyl, 2-cthylpropenyl, pentenyl and hexenyl. Unless otherwise stated, "alkinyl" includes straight-chain or branched-chain alkinyl groups with up to 26 carbon atoms. Cycloalkyl preferably refers to a C3-C7-cycioalkyl that may be substituted; alkyl, alkoxy (e.g. methoxy, ethoxy, etc.), halogen (e.g. fluorine, chlorine, bromine, etc.), nitro and the like are suitable as possible substituents. Aryl is an aromatic hydrocarbon residue, such as phenyl, naphthyi, etc., which may have one or more suitable substituents such as alkoxy (e.g. methoxy, ethoxy, etc.), halogen (e.g. fluorine, chlorine, bromine, etc.), nitro and the like. ■ i "Aralkyl" includes mono-, di- and triphenylalkyls such as benzyl, phenethyl, benzhydryl, trityl and the like, where the aromatic part may have one or more suitable substituents such as alkoxy (e.g. methoxy, etiioxy, etc.), halogen (e.g. fluorine, chlorine, bromine, etc.), nitro and the like. "Alkoxy residue" relates to a straight-chain or branched-chain alkoxy residue with up to 26 carbon atoms, such as methoxy or ethoxy residues, etc., unless otherwise stated. It can be substituted for example by hydroxyl, amino, halogen and oxo groups and alkoxy residues such as methoxy or ethoxy residues. Unless otherwise stated, "hydroxymethyl residue" relates to a residue that has a substituted or unsubstituted Ci-Cg-alkyl, aryl or aralkyl residue, e.g. methoxymcthyl, ethoxymethyl, phenoxymethyl or bcnzoxymethyl, etc., attached lo the oxygen, or has a substituted or unsubstituted phosphate or pyrophosphate residue, such as adenosine diphosphate, uridine diphosphate, etc., attached to the oxygen. Unless otherwise stated, "alkylthio residue" relates to a straight-chain or branched- chain alkylthio residue with up to 9 carbon atoms, such as thiomethyl or thiocth) residues, etc. It can be substituted e.g. by hydroxyl, amino, halogen and oxo group and alkoxy residues such as methoxy or cthoxy residues. "Silyl residues" can, for example, be substituted by the above-defined alk) residues or cycloalkyl-(Co-2(>)-alkyl residues. "Silyl-(Co-26)-alkyl groups" are silyl residues that can also be bonded to th framework by means of an alkyl residue. The alkyl and silyl groups are defined a above. The alkane and/or arene parts may, if desired, have at least one suitable substituen such as a halogen, alkoxy, hydroxyl, nitro or the like, in the case of th aforementioned esters. Substituted and unsubstituted phosphate residues or substituted and unsubstitutoi pyrophosphate residues include salt compounds of the corresponding phosphori acid derivatives with organic or inorganic bases (e.g. sodium salt, potassium sail calcium salt, aluminium salt, ammonium salt, magnesium salt, triemylamine sail ethanolamine salt, dicyclohcxylaminc salt, cthylenediamine salt, N,N'-dibenzyl ethylcncdiaminc salt, etc.), as well as amino acid salts (e.g. argininc salt, lysin salt, glycine salt, alanine salt, ornithine salt, etc.), and also residues in which th phosphate group forms esters with substituted or unsubstituted Ci-C2&-alkyl substituted or unsubstituted aryl, substituted or unsubstituted aralkyl, substituted o unsubstituted cycloalkyl or a substituted or unsubstituted heterocyclic residue, o with a nucleoside or a deoxynueleoside. "Nucleoside" is understood as meaning adenosine, guanosine, uridine, thymidin and cytidinc, while "deoxynueleoside" is understood as meaning deoxyadenosinc deoxyguanosine, deoxythymidine, deoxycytidine and deoxyuridine. The invention also relates to the pharmaceutical salts and esters of the salts Moreover, it includes all spatial isomers of the compounds, both as pure substance and as mixtures thereof. The substances according to the invention can be obtained from bacteria, algae, plants and protozoa, including those in which the lytB gene has been deleted, and purified (Example 1). Purification can be effected by means of HPLC or by other methods known per se, such as electrophoresis, precipitation (e.g. as a barium salt) or other chromatographic techniques. Various applications of the compounds are possible. Accordingly, it has been shown, for example, that the substances can be employed in the activity testing of the enzymes GcpE and LytB, as well as in test systems for the measurement of j gamma/delta T-cell activation (see Examples 2, 5). The substances according to the invention can either.be chemically synthesized (Example-3) or be obtained from bacteria, algae, plants and protozoa and purified (Example 4). Purification can be effected by means of HPLC or by other methods known per se, such as electrophoresis, precipitation (e.g. as a barium salt) or other chromatographic techniques. Furthermore, the substances according to the invention can be used in a screening procedure for the identification of GcpE and LytB enzyme inhibitors, as they are intermediates of the MEP. This method of determining the activity of the enzymes is based on the measurement of differences in the concentration of the enzyme substrates and products under suitable reaction conditions. By bringing suitable test substances into contact with the enzymes during activity determination, inhibitors can be identified by the reduction in the observed enzyme activity. The inhibitors are suitable as herbicides and as active ingredients with antibacterial, antiparasitic and antiviral activity in humans and animals. The compounds according to the invention can also be used in the production of medicines. The efficacy of the compounds is based on the activation of gamma/delta T-cells. Depending on the field of application, the immunological defence mechanism can thereby be strengthened or an immunological tolerance can be induced towards autoantigens and allergens. The fields of application are the treatment of immune and autoimmune diseases and allergies in humans and animals. Examples of these are: allergies, multiple sclerosis, rheumatoid arthritis, Hashimoto's thyroiditis, myasthenia gravis, lupus erythematosus, diabetes mellitus, primary biliary cirrhosis, active chronic hepatitis, adrenalitis/Addison's disease, polymyositis, dermatomyositis, autoimmune haemolytic anaemia, myocardial inflammation and inflammation of the heart membrane, scleroderma, uveitis (phacouveitis, sympathetic ophthalmia), pemphigus vulgaris, pemphigoid, pernicious anaemia, autoimmune atrophic gastritis, inflammatory diseases of the intestine, such as Crohn's disease and ulcerative colitis, and inflammatory diseases of the lung, such as asthmatic and bronchitic ailments. The preferred fields of application are Crohn's disease, ulcerative colitis, multiple sclerosis, asthma, chronic bronchitis and allergies. Furthermore, it has been shown that the substances according to the invention can be successfully employed in the treatment of diseases which are caused by viruses, bacteria and parasites. In particular, the substances defined in claim 1 and the subordinate claims are suitable for the prevention and treatment of tumours that are caused by microorganisms. Bacteria, such as Helicobacter pylori (e.g. tumours of the gastrointestinal tract), and papilloma viruses (e.g. tumours of the female genitalia), belong to this group of microorganisms. The compounds defined in the claims are particularly suitable for the prophylaxis and treatment of one of the aforesaid diseases as well as hepatitis C virus infections and benign and malignant tumours, particularly those caused by papilloma viruses, and for helicobacter eradication therapy in cases of ulceration of the gastrointestinal tract. For medicinal purposes, pharmaceutical preparations can be used on their own or in combination with other medicines and can contain either the isolated substances according to the invention or living or dead organisms containing the substances. They are preferably used in combination with substances that are recognized by the immune system as being foreign antigens or autoantigens. Examples of these are myelin basic protein (MBP) and other extracts of the tissue of the nervous system, type I, II or 111 collagen, thyroglobulin, acetylcholine receptor protein, DNA, islet cell extracts, human insulin, liver extracts, hepatocellular extracts, adrenocortical extracts, skin extracts, heart extracts, muscle extracts, skin cell extracts, haemopoietic line cell extracts, eye lens proteins, S-antigens, S-antigen mixtures, stomach cell extracts, parietal cell extracts, intrinsic factor and intestinal extracts. Preferred forms of administration are oral, inhalational, intravenous, parenteral, intracisternal, intravaginal, intraperitoneal, local (powder, ointment, drops) and rectal administration, as well as application to the skin or mucous membranes. The invention includes the administration of an inhalant containing at least one of the substances denned in claim 1 for the treatment of human diseases, particularly allergies and diseases of the respiratory tract such as asthma and chronic bronchitis. Suitable pharmaceutical compositions arc moreover: tablets, retard tablets, dragecs, capsules, premixes, pills, pellets, boli, aerosols, granules, suppositories, solutions, concentrates, suspensions and emulsions, pastes, ointments, gels, creams, lotions, powders, infusions and sprays. The pharmaceutical formulations may correspond to a fraction or a multiple of a single dose. Dosage units can be 1, 2, 3 or 4 times a single dose, for example, or may contain 1/2, 1/3 or 1/4 of a single dose. A single dose preferably contains the quantity of active ingredient which is used for one administration and which usually corresponds to a whole, a half, a third or a quarter of the daily dosage. Tablets, dragecs, capsules, pills and granules may contain the active ingredients in addition to the usual excipients such as (a) fillers and diluents, e.g. starches, lactose, cane sugar, glucose, mannitol and silicic acid, (b) binders, e.g. carboxymethyl cellulose, alginates, gelatine and polyvinylpyrrolidone, (c) humectants, e.g. glycerine, (d) disintegrating agents, e.g. agar-agar, calcium carbonate and sodium carbonate, (e) solution retarders, e.g. paraffin, and (f) absorption accelerators, e.g. quaternary ammonium compounds, (g) wetting agents, e.g. cetyl alcohol and glycerol monostearate, (h) adsorbents, e.g. kaolin and bentonite, and (i) lubricants, e.g. talcum, calcium and magnesium stearate and solid polyethylene glycols, or mixtures of the substances listed under (a) to (i). Moreover, the compounds according to the invention can also be incorporated into other carrier materials such as plastics (plastic chains for local therapy), collagen or bone cement. The tablets, dragecs, capsules, pills and granules may be provided with the usual coatings and envelopes optionally containing opaquing agents, and can be prepared in such a way that the active ingredients are released, optionally with a delay, only in the intestinal tract or, preferably, in a particular part of the intestinal tract, it being possible to use e.g. polymer substances and waxes as embedding compounds. The active ingredients can also be in microencapsulated form, optionally with one or more of the aforesaid excipients. In addition to the active ingredients, suppositories may contain the usual water-soluble or water-insoluble excipients, e.g. polyethylene glycols, fats, e.g. cacao fat, and higher esters (e.g. a C14alcohol with a C16 fatty acid), or mixtures of these substances. In addition to the active ingredient(s), ointments, pastes, creams and gels can contain the usual excipients, e.g. animal and vegetable fats, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acids, talcum and zinc oxide, or mixtures of these substances. In addition to the active ingredient(s), powders and sprays may contain the usual excipients, e.g. lactose, talcum, silicic acid, aluminium hydroxide, calcium silicate and polyamide powder, or mixtures of these substances. Additionally, sprays may also contain the usual propellant, e.g. chlorofluorocarbons. In addition to the active ingredients, solutions and emulsions may contain the usual excipients such as solvents, solubilizers and emulsifying agents, e.g. water, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformainide, oils, particularly cottonseed oil, peanut oil, maize oil, olive oil, castor oil and sesame oil, glycerine, glycerol formal, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitol, or mixtures of these substances. There are very marked differences in the amounts of the individual derivatives that are necessary in order to achieve the desired effect. Generally speaking, both in human as well as in veterinary medicine, it has proved to be advantageous if the active ingredient(s) of fomiula (I) are administered in total amounts of approximately 0.01 to about 2000 ug every 24 hours, if necessary in the form of several single doses, in order to achieve the desired results. A single dose preferably contains the active ingrcdient(s) in amounts of approximately 0.01 to about 2000 ug. However, it may be necessary to deviate from the abovementioned dosages, depending on the type and body weight of the person to be treated, the nature and severity of the disease, the type of preparation and the administration of the medicine, as well as the period or interval over which the preparation is administered. Consequently, it may be sufficient in a number of cases to manage with less than the abovementioned quantity of active ingredient, while in other cases the afore¬mentioned quantity of active ingredient will have to be exceeded. 'The optimum dosage required and the type of administration of the active ingredients can be determined by those skilled in the art on the basis of their specialist knowledge. In the treatment of animals, the compounds to be used according to this invention can be given in the usual concentrations and preparations together with the food or food preparations or with the drinking water. Example 1 Purification of gamma/delta T-ccIl activating compounds Various gamma/delta T-cell activating compounds were isolated from Coryne-bacterium ammoniagenes. 28 kg of the cell mass were digested with a Dynax mill in 50 mM ammonium formate buffer (pH 8.0). After preabsorption on : hydrophobic polystyrene matrix, the digested material was loaded onto an anioi exchanger and elutcd with a stepped gradient (100, 300, 500 mM ammoniun formate, pH 8.0). The 300 mM eluatc was passed through a C-18 matrix and thei through a 3 kDa hollow fibre filter for ultrafiltration. The filtrate was diluted will water to 30 mM ammonium formate and loaded once more onto an anioi exchanger. Elution then took place with a linear gradient of 30 to 500 mtv ammonium formate. Individual fractions were tested for their ability to activate gamma/delta T-cells. Then some of the active compounds were precipitated a barium salts by the admixture of 100 mM BaCh and 80% EtOH. The precipitate were dissolved in 20 mM ammonium formate buffer (pH 8.0) am rechromatographed on an anion exchanger. In this way it is possible to isolate compounds 1 to 6. Example 2 Activation of gamma/delta T-cells by enriched MEP intermediates Lymphocytes were obtained from the peripheral blood of healthy donors by Ficol density gradient centrifugation. For each test, 2 x 105 of tne cells so obtained wen seeded in a volume of 0.2 ml of RPMI-1640 medium (Life Technologies) that wa: nriched with 25 mM HEPES, 2 mM L-glutamine, 0.025 mg/ml of gentamicin, 10( U/ml of human interleukin-2 (IL-2) (all from Life Technologies) and 10% humai AB serum (Bavarian Red Cross). The test fractions were added in variou: dilutions, and isopentcnyl diphosphate (IPP) from Sigma was used in a Una concentration of 10 uM as a positive control. Incubation was effected in th The results showed that compound 1 was approximately 750 times more activ Example 3 The synthesis was effected in the manner described in diagram 1: Diagram 1: Synthesis plan 1. Preparation of compounds la to Ic Compounds la and lb were prepared in an analogous manner to that described in K. Sato, S. Inoue, Y. Takagi, S. Morii, Bull. Chem. Soc. Jpn., 1976, 49(11), 3351 -3351. The preparation of compound Ic is analogous to that described in H. Kunio, 11. Kazushige, Chem. Pharm. Bull., 1994, 42, 4, 786-791. 2. Syntheses of compounds Ila to11e ^ Compound Ila was prepared according to current methods which are known to those skilled in the art, such as have been described in e.g. B. Woodside, Z. Huang, C. Poulter, Org. Synth. 1988, 66, 211-219, starting from compound lb. Compound lib was prepared starting from compound la. la was first converted to the corresponding tosylate and then reacted e.g. with tris(tetra-n-butylammonium) hydrogenomethylenediphosphate. The synthesis was carried out in a manner analogous to that described in WO00/59916 and the publications cited therein. Compound 11Cin turn was prepared from compound lc. The syntheses were carried out in the manner described in R.C. McClard and T.S. Fujita, J. Am. Chem. Soc, 1987,109,5544-5545. Compound 11Ccould be obtained in a low yield and was immediately hydrolysed in order to obtain compound IIIc. 3. Syntheses of compounds IIIa TOIIIc In order to prepare compounds IIIa to IIIc, 500 mg of the corresponding precursors IIa and IIb were each dissolved in 5 ml of methanol and treated with 10 mol% of hydrogenation catalyst. Then hydrogen was introduced at room temperature and the uptake of hydrogen was measured. After the appropriate amount of hydrogen had been taken up, the mixture was filtered and the solvent was stripped off. The required products IIa and IIIb were obtained with a good degree of purity. Further purification can be achieved by chromatographic methods. Compound lite was obtained from compound 11C. 200 mg of compound 11e were dissolved in absolute methylene chloride (3 ml) in a heated, argon-flushed flask and 10 eq. of trimethylbromosilane were added at 0°C. After stirring for one hour at 0°C, stirring was continued for a further 12 h at room temperature. Finally, an aqueous work-up yielded the required product lllc, which was purified by ion exchange chromatography. In order to test the activation of gamma/delta T-cells, either the isomcrically pure compounds or E/Z mixtures of the compounds were used. Example 4 Purification of gamma/delta T-cell activating compounds Various gamma/delta T-ccll activating compounds were isolated from Coryne-bacterium ammoniagenes. 28 kg of the cell mass were digested with a Dynax mill in 50 mM ammonium formate buffer (pH 8.0). After preabsorption on a hydrophobic polystyrene matrix, the digested material was loaded onto an anion exchanger and eluted with a stepped gradient (100, 300, 500 mM ammonium formate, pH 8.0). The 300 mM eluate was passed through a C-18 matrix and then through a 3 kDa hollow fibre filter for ultrafiltration. The filtrate was diluted with water to 30 mM ammonium formate and loaded once more onto an anion exchanger. Elution then took place with a linear gradient of 30 to 500 mM ammonium fomiate. Individual fractions were tested for their ability to activate gamma/delta T-cells. Then some of the active compounds were precipitated as barium salts by the admixture of 100 mM BaCb and 80% EtOH. The precipitates were dissolved in 20 mM ammonium formate buffer (pH 8.0) and rechromatographed on an anion exchanger. In this way it was possible to isolate compounds 1 to 6, 13 and 14. i Example 5 Activation of gamma/delta T-cclls by enriched MEP intermediates Lymphocytes were obtained from the peripheral blood of healthy donors by Ficoll density gradient centrifugation. For each test, 2 x 105 of the cells so obtained were seeded in a volume of 0.2 ml of RPMI-1640 medium (Life Technologies) that was enriched with 25 mM HEPES, 2 mM L-glutamine, 0.025 mg/ml of gentamicin, 100 U/ml of human interleukin-2 (IL~2) (all from Life Technologies) and 10% human AB serum (Bavarian Red Cross). The test fractions were added in various dilutions, and isopentenyl diphosphate (IPP) from Sigma was used in a final concentration of 10 uM as a positive control. Incubation was effected in the incubator with 5% CO2 at 37°C. Alter 72 hours the cells were harvested and analysed in a flow cytometer. In so doing, the expression of the CD25 activation marker on the surface of V gamma 9f T-cells was measured with the aid of the monoclonal antibodies CD25-PE (B 1.49.9), V gamma 9-FITC (lmrnu360) and CD3-PC5 (UCHT1) supplied by Beckman-Coulter. WE CLAIM : 1. A method of preparing gamma/delta T-cell activating compounds having formula as depicted in any one of formula (I), (IIA), (IIB), (IIC) and (III) characterized in that, in Corynebacterium ammoniagenes, deleting the LytB gene, inactivating or changing in known manner, enzymatic activity of the gene product, and thereby enriching the compounds by isolating from cells of Corynebacterium ammoniagenes wherein digesting the cell mass in 50 mM ammonium formate buffer (pH 8.0) with Dynax mill; eluting digested material onto an anion exchanger with stepped gradient (100, 300, 500 mM ammonium formate buffer, pH 8.0) after preabsorption on a hydrophobic polystyrene matrix; ultra-filtering the elute of 300mM by passing through a C-18 matrix and through a 3 Kda hallow fibre filter; further eluting the filtrate after ultra-filtration on anion exchanger by diluting with water to 30 mM ammonium formate and precipitating barium salt from the said eluted fraction by admixing 100 mM BaCh and 80% ethanol. Dated this on 13th day of January 2004 HIRAL CHANDRAKANT JOSHI AGENT FOR BIO AGENCY AG, GERMANY

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