Title of Invention

PROCESS FOR THE PREPARATION OF SPIRO[(4-CYCLOHEXANONE)-[3H]INDO]-2' [1 'H]-ONE DERIVATIVES

Abstract

Process for the preparation of spiro[(4-cyclohexanone)-[3H]indo]-2' [l'H]-one derivatives of the general formula I wherein R1 and R2 independently stand for hydrogen or C1-4 4alkoxy-, characterizead by reacting an indolin-2-one derivative of the general formula II (II) wherein R1 and R2 are as defined above - with a compound capable for introducing a protective group, selected from 2- tetrahydropyranyll, 1-diethoxymethylene or C1-4 alkoxycarbonylethyl group, coupling the compound of general formula III, (III) thus obtained - wherein R1 and R2 are as defined above and A stands for a protective group, selected from 2- tetrahydropyranyl, 1 -diethoxy-methylene or C1-4 alkoxycarbonylethyl group - with an acrylic acid C1-4 ester, cyclizing the resulting compound of the general formula IV (IV) wherein R1, R2 and A are as defined above, R3 stands for C1-4 alkyl group-, eliminating the -COOR3 group and the A protective group of the keto-ester of general formula V wherein R1, R2, R3 and A are as defined above, with or without isolation of the compounds of the general formulae IV and /or V (v) and/or VI

Full Text

FORM 2 THE PATENTS ACT 1970 [39 OF 1970] COMPLETE SPECIFICATION [See Section 10] PROCESS FOR THE PREPARATION OF SPIRO [(4- CYCLOHEXANONE)-[3H]INDOL]-2'[l'H]-ONE DERIVATIVES" SANOFI-SYNTHELABO, pf 174, Avenue de France, F-75013 Paris, France, The following specification particularly describes the nature of the invention and the manner in which it is to be performed:- The present invention relates to a process for the preparation of spiro[(4-cyclohexanone)-[3H]indo]-2' [l'H]-one derivatives. Spiro[(4-cyclohexanone)-[3H]indol-2'[lH]-one and dispiro[(l,3-dioxolan)-2,4'-cyclohexane-[3H]indol]-2"[lH]-one derivatives are important intermediates to the vasopressine V2 antagonistic compound, SR 121463. For example, as described in patentapplication WO9715556 the dispiro[(l,3~dioxolan)~2,4'-cyciohexane-l,3"-(5"-ethoxy)-[3H]indol]-2"[lH]-one (compound of formula VII) H • . can be prepared by reacting 4-ethoxyphenylhydrazine with 4-(l,3- dioxolan)cyclohexane-carboxylate sodium salt followed by cyclization of the resulting 1 -(4 '-ethoxyphenyl)-2-(4"-/l ,3~dioxolan/-cyclohexane-carbonyl)- hydrazine. According to another synthetic route (EP 636608) the compound of formula VII is obtained by oxidation of the spiro[(4-hydroxy-cyclohexane)-l,3'(5'-ethoxy)-[3H]indol-2'[1H]-one to the appropriate cyclohexanone derivative, from which the ketale of the formula VII is prepared by reaction with ethylene glycol. Disadvantages of both of the above syntheses are the toxic starting materials, many-step syntheses, low yields of some synthetic steps, expensive reagents and extreme reaction conditions in certain reactions. To our surprise, we have found that in contrast to the analogous reaction described in the literature (Annalen 1941, 548, 117-146; J. Am. Chem. Soc. 1953, 75, 5301-5305; L Chem. Soc. C, 1970, 796-800, J. Med. Chem. 1993, 36, 2459-2469) the addition of methyl or ethyl acrylate to 5-ethoxy-indolinone, followed by 00000008 Dieckmann-condensation, hydrolysis and decarboxylation does not lead to a homogeneous product, that is why the procedures described in the literature above are not suitable for industrial synthesis. We have found that the hydrogen in position-1 of the 5-ethoxy-indolinone has to be substituted by an appropriate protective group, if we want the subsequent reactions to proceed in the desired direction. In the case the substituent in position-1 is a phenyl group as described in USP 3395156 side reactions cannot be avoided. Before the Dieckmann-condensation the l|0 obtained l-phenyloxindole-3,3-dipropionic acid ester has to be hydrolyzed and reesterified to get a pure starting material. The subject of our invention is a process for the preparation of spiro[(4-cyclohexanone)-[3H]indol]-2' [l'H]-one derivatives of the general formula I I H . - wherein R1 and R2 independently stand for hydrogen, C1-4alkyl, C1-4alkoxy, C1-4alkylthio, C1-4 polyfluoroalkyl, C1-4 polyfluoroalkoxy, C3-7cycloalkyloxy, C3-7cycloalkylthio, phenoxy, benzyloxy or nitro group -, characterized by reacting an indolin-2-one derivative of the general formula II 20 - wherein R1 and R2 are as defined above - with a compound capable for introducing a protective group, selected from 2-tetfahydropyranyl, 1-diethoxy-methylene or C1-4alkoxycarbonylethyl group, coupling the compound of general HU 00000008- formula III, thus obtained - wherein R1 and R2 are as defined above and A stands for a protective group, selected from 2-tetrahydropyranyl, 1-diethoxy-methylene or C1-4alkoxycarbonylethyl group - with an acrylic acid C1-4ester, cyclizing the resulting compound of the general formula IV - wherein R1, R2 and A are as defined above, R3 stands for C1_4 alkyl group-, eliminating the -COOR3 group and the A protective group of the keto-ester of general formula V wherein R1, R2, R3 and A are as defined above, optionally without isolation of the compounds of the general formulae IV '19-06-2001 HU 00000008' and/orV and/or VI. As for compounds capable to introduce the A protective group 2,3-dihydropyrane, triethyl orthoformate or an acrylic acid C1-4ester can be applied. The reaction of the compound of general formula II - wherein R1 and R2 are as defined above - with the compound capable to introduce the A protective group is preferably carried out in the presence of a catalyst, preferably in the presence of p-toluenesulfonic acid. As for solvent halogenated hydrocarbons, preferably dichloromethane can be used. HU oooooooe "The reaction of the compounds of general formulae II or III .1 - wherein R1R2 and A are as defined above - with the acrylic acid C1-4ester is carried out in the presence of a catalyst, preferably in the presence of an alkali alcoholate, favorably sodium alcoholate. Cyclization of the compounds of general formula IV A is carried out in the presence of an alkali alcoholate, preferably in the presence of sodium ethylate, potassium t-butylate. Using acrylic acid C1-4ester for protective group, the process can advantageously performed as a "one pot" method, in a polar solvent, in the presence of a base. Compounds of the general formulae III HU 000000081 IV, V and VI - wherein R , R , R and A are as defined above - are new compounds, with the proviso, that in compound IV, if R1 and R2 stand for hydrogen, A is different from methoxycarbonyl-ethyl group. Further details of the invention are demonstrated by the following examples, without limiting the claims to the examples. HU 00000008 Examples 1./ To 38.33 g of 5-ethoxy-indan-2-one 2.12 g of p-toluenesulfonic acid and 880 ml of dichloromethane are added, then under stirring 59 ml of dihydropyrane. The reaction mixture is stirred until complete dissolution (approx. 2 hours), then it is allowed to stand for 36 hours. The resulting brown solution is washed with 8 % aqueous sodium hydrogencarbonate solution, dried over sodium sulfate and evaporated. The residue is slowly poured into 500 ml of petroleum ether. The resulting precipitate is filtered off, washed with a small amount of petroleum ether. Thus, 42.4 g of l-(2-tetrahydropyranyl)-5-ethoxy-indolin-2-one is obtained, mp.: 108-110 °C. Yield: 75% Tp-41.8 g of l-(2-tetrahydropyranyl)-5-ethoxy-indolin-2-one, 1.2 g of sodium ethylate and 440 ml of toluene are added. To the resulting solution 34 ml of ethyl acrylate is added slowly, under stirring, at 25 °C, in a period of 4 hours. The reaction mixture is stirred for additional 2 hours, then it is washed with 8 % aqueous sodium hydrogencarbonate solution, dried over sodium sulfate, clarified with active carbon, filtered and concentrated in vacuo. The residue is slowly poured into 250 ml of petroleum ether. The resulting solid material is filtered off, washed with petroleum ether. 58 g of l-(2- tetrahydropyranyl)-3 -(di-/ethoxycarbonylethyl/)-5-ethoxy-indolin-2-one is obtained, mp,:84-86oC:78%. 178 g of l-(2-te1rahydropyranyl)-3-(di-/ethoxycarbonylethyl/)-5-ethoxy-indolin-2- one in 1300 ml of toluene is stirred with 60.4 g of sodium ethylate and 6 g of tetrabutylammonium bromide at 55 °C for 3,5 hours. After cooling the reaction mixture is extracted consecutively with 300 ml of ice-cold water, 300 ml of 1 N hydrochloric acid, and 150 ml of water, clarified with active carbon and Fuller's earth, and filtered. The filtrate is evaporated in vacuo, the residue is heated under stirring at reflux temperature for 3.5 hours in the mixture of 790 ml of 50 %-ethanol and 315 ml cone, hydrochloric acid. The mixture is then poured into 3000 HU 00000008: 2) 25 30 ml of water, the aqueous phase is extracted with 2x600 ml and 3x300 ml of toluene, dried over sodium sulfate, evaporated in vacuum. The residue is crystallized in diisopropyl ether. The resulting material is filtered off, washed with diisopropyl ether. Thus 60.1 g of spiro[(4-cyclohexanone)-l,3'(5'-ethoxy)-[3H]indol-2'[l'H]-one is obtained, mp,;171- 172oC, -Yield: 60 %. 2.1 Into 318 ml of dimethyl sulfoxide 112.7 g of 5-ethoxy-indan-2-one is added, then, under stirring, 3.82 g of potassium t-butylate. To that suspension, after 10 minutes of stirring, 172.1 g of methyl acrylate is added, dropwise, at a temperature of 40 - 45 °C, in a period of 70 minutes. The mixture is stirred at that temperature for additional 65 minutes, then to it is added 161 g of potassium t-butylate, in a period of 30 minutes, while keeping the temperature below 60 °C. The t-butanol is distilled off, the thick residue is poured into 1780 ml of water, the solution is clarified with active carbon and filtered. The filtrate is stirred in a 85 °C bath. When it reaches the 68 °C (approx. 25 min.) it is seeded, then stirring is continued for additional 3 hours, at a temperature of max. 81 °C. The mixture is then cooled to room temperature, the resulting precipitate is filtered off and washed thoroughly with water. Thus, 110.7 g of spiro[(4-cyclohexanone)-l,3'(5'-ethoxy)-[3-H]indol-2'[lH]-one is obtained, mp,:184-186°C, it suitable for the next step. Yield: 67 %. 3./ 11.2 g of 5-ethoxy-indan-2-one and 220 ml of triethyl orthoformate are stirred at 135-140° C for 20 hours, then the reaction mixture is evaporated in vacuo. 17.2 g of l-(diethoxy-methylene)-5-ethoxy-indolin-2-one is obtained as an oil. Its structure was proved by NMR spectroscopy. Yield: 92 %. To the mixture of 17.2 g of l-(diethoxy-methylene)-5-ethoxy-indolin-2-one, 1.5 g of potassium /-butylate and 170 ml of toluene 12.6 ml of ethyl acrylate is added dropwise, at 20 - 30°C, in a period of 1 hour. After additional 90 minutes of stirring 50 ml of water is added to the reaction mixture, the phases are separated, the organic phase is washed with water, dried over sodium sulfate, evaporated in vacuum. 22.2 g of l-(diethoxy-methylene)-3-(di-/ethoxycarbonylethyl/)-5-ethoxy- HU 000000081 indolin-2-one is obtained in the form of yellow-brown crystallizing oil, which is crystallized from 110 ml of n-hexane. 15.1 g crystalline product is obtained, mp: 82-83°C. Yield: 62 %. 15 g of l-(diemoxy-methylene)-3-(di-/ethoxycarbonylethyl/)-5-ethoxy-indolin-2-one is dissolved in 150 ml of toluene, and to the solution 7.2 g of potassium-t-butylate is added under stirring, in 10 minutes. The reaction mixture is stirred at room temperature for 2 hours, then water is added to it, the phases are separated, the organic phase is washed with water, dried over sodium sulfate, evaporated in vacuum. Thus, 10.2 g of spiro[(3-ethoxycarbonyl-4»cyclohexanon)-l,3'(l'-diethoxy-methylene-5,-ethoxy)-[3ifjindol]-2'-one is obtained in the form of a brown oil, its structure was proved by NMR spectroscopy. Yield: 76 %. 4,05 g of spiro[(3-ethoxycarbonyl-4-cyclohexanon)-l,3'(l '-diethoxy-methylene-5'-ethoxy)-[3H]indol-2'-one is stirred for 2 hours at room temperature in the mixture of 20 ml of 96 % ethanol and 0,5 ml of 2N hydrochloric acid, the reaction mixture is then cooled with ice-water to 5°C. The precipitating spiro[(3-ethoxycarbonyl-4-cyclohexanon)-l,3'(l'-formyl-5'-ethoxy)-[3H]indol]-2'-one is filtered off, mp.: 133 -136 °C. Yield: 52 %. 5 g of spiro[(3-ethoxycarbonyl-4-cyclohexanon)-l,3'(l'-formyl-5'-ethoxy)- [3H]indol]-2'-one is dissolved in 100 ml of acetic acid, 25 ml of 5N sulfuric acid is added to it. The mixture is refluxed under stirring, then it is evaporated in vacuum. To the residue water is added, the pH is adjusted to pH 7 with sodium hydroxide solution. 3.1 g of solidifying oil precipitates is obtained, which is identical with the spiro[(4-cyclohexanon)-l,3'(5'-ethoxy)-[3//]indol]-2'[l'H]-one obtained by another route. Mp:139-1400-C Yield: 86 %. Claim: - 1. Process for the preparation of spiro[(4-cyclohexanone)-[3H]indo]-2' [l'H]-one derivatives of the general formula I wherein R1 and R2 independently stand for hydrogen or C1-4 4alkoxy-, characterizead by reacting an indolin-2-one derivative of the general formula II (II) wherein R1 and R2 are as defined above - with a compound capable for introducing a protective group, selected from 2- tetrahydropyranyll, 1-diethoxymethylene or C1-4 alkoxycarbonylethyl group, coupling the compound of general formula III, (III) thus obtained - wherein R1 and R2 are as defined above and A stands for a protective group, selected from 2- tetrahydropyranyl, 1 -diethoxy-methylene or C1-4 alkoxycarbonylethyl group - with an acrylic acid C1-4 ester, cyclizing the resulting compound of the general formula IV (IV) wherein R1, R2 and A are as defined above, R3 stands for C1-4 alkyl group-, eliminating the -COOR3 group and the A protective group of the keto-ester of general formula V V wherein R1, R2, R3 and A are as defined above, with or without isolation of the compounds of the general formulae IV IV and /or V (v) and/or VI Vl Process as claimed in claim 1 wherein 2, 3-dihydropyrane, triethylorthoformate or acrylic acid C1-4 ester are compounds capable for introducing the A protective group. Process as claimed in claim 1, wherein the reaction of the compound of the general formula II (II) wherein R1 and R2 are as defined in claim 1, is carried out with the compound capable for introducing the A protective group - wherein A is as defined in claim 1 - in the presence of a catalyst. Process as claimed in claim 1, wherein the reaction of the compound of the general formula III III wherein R1, R2 and A are as defined in claim 1 is carried out with an acrylic acid C1-4 ester in the presence of a catalyst. 5. Process as claimed in claim 3, wherein the catalyst is an acidic catalyst, preferably p-toluenesulfonic acid or an alkaline catalyst, preferably an alkali alcoholate. 6. Process as claimed in claim 4, wherein the catalyst is an alkaline catalyst, preferably alkali alcoholate. Dated this the 20th day of December, 2001 [JAYANTA PAL] Of Remfry & Sagar ATTORNEY FOR THE APPLICANTS]

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