Title of Invention	METHOD OF PRODUCING TOBACCO HAVING LOW LEVELS OF NITROSAMINES
Abstract	The present invention discloses a method of producing tobacco having low levels of nitrosamines, comprising: subjecting a harvested tobacco plant ,or portion, thereof,to microwave energy, while said plant is uncured ,yellow, and in a state susceptible to having the formation of nitrosamines arrested, for a sufficient time to substantially prevent formation of nitrosamine

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Field of the Invention The present invention relates to a method of producing tobacco having low levels of nitrosammes. Cross-reference to Related Application This is application is a continuation-in-part of application Serial No. 08/757,104 filed December 2, 1996, which is a continuation-in-part of application Serial No. 08/739,942 filed October 30, 1996, which is a continuation in part of application Serial No. 08/725,691, filed' September 23, 1996, which is a continuation-in-part of Application Serial No. 08/671,718, filed June 28, 1996. The present application and the applications recited above, with the exception of Application Serial No. 08/671,718, filed June 28, 1996, claim priority to provisional application Serial No. 60/023,205, filed August 5, 1996. Background Of The Invention Others have described the use of microwave energy to dry agricultural products. Use of microwave energy to cure tobacco is disclosed in U.S. Patent No. 4,430,806 to Hopkins. In U.S. Patent 1A No. 4,898,189, Wochnowski teaches the use of microwaves to treat green tobacco in order to control moisture content in preparation for storage or shipping. In U.S. Patent No. 3,699,976, microwave energy is described to kill insect infestation of tobacco. Moreover, techniques using impregnation of tobacco with inert organic liquids (U.S. Patent No. 4,821,747) for the purposes of extracting expanded organic materials by a sluicing means have been disclosed wherein the mixture was exposed to microwave energy. In another embodiment, microwave energy is disclosed as the drying mechanism of extruded tobacco-containing material (U.S. Patent No. 4,874,000). In U.S. Patent No. 3,773,055, Stungis discloses the use of microwave to dry and expand cigarettes made with wet tobacco. Prior attempts to reduce tar and harmful carcinogenic nitrosamines primarily have included the use of filters in smoking tobacco. In addition, attempts have been made to use additives to block the effects of harmful carcinogens in tobacco. These efforts have failed to reduce the oncologic morbidity associated with tobacco use. 31 is known that fresh-cut, green tobacco has virtually no nitrosamine carcinogens. See, e.g., Wiernik et al, "Effect of Air-Curing on the Chemical Composition of Tobacco," Recent Advances in Tobacco Science, Vol. 21, pp. 39 et seq Symposium Proceedings 49th Meeting Tobacco Chemists' Research Conference, Sept. 24-27, 1995, Lexington, Kentucky (hereinafter "Wiernik et al") . However, cured tobacco is known to contain a number of nitrosamines, including the harmful carcinogens N' - 2 nitrosonornicotine (NNN) and 4-(N-nitrosomethylamino)-l-(3-pyridyl)-1-butanone (NNK). it is widely accepted that such nitrosamines are formed post-harvest, during the curing process, as described further herein. Unfortunately, fresh-cut green tobacco is unsuitable for smoking or other consumption. In 1993 and 1994, Burton et al at the University of Kentucky carried out certain experiments regarding tobacco-specific nitrosamines (TSNA), as reported in the Abstract, "Reduction of Nitrite-Nitrogen and Tobacco N'-Specific Nitrosamines In Air-rCured Tobacco By Elevating Drying Temperatures", Agronomy & Phytopathology Joint Meeting, CORESTA, Oxford 1995. Burton et al reported that drying harvested tobacco leaves for 24 hours at 7l°c, at various stages of air curing, including end of yellowing (EOY), EOY+3, EOY+5, etc. resulted in some reduction of nitrosamine levels. Reference is also made to freeze drying and microwaving of certain samples, without detail or results. Applicant has confirmed that in the actual work underlying this Abstract, carried out by Burton et al at the University of Kentucky, the microwave work was considered unsuccessful. Certain aspects of Burton et al's 1993-94 study are reported in Wiernik et al, supra, at pages 54-57, under the heading "Modified Air-Curing". The wiernik et al article postulates that subjecting tobacco leaf samples, taken at various stages of air-curing, to quick-drying at 70°C for 24 hours, would remove excess water and reduce the growth of microorganisms; hence, nitrite and tobacco-specific nitrosamine (TSNA) accumulation would be avoided. In Table II at page 56, Wiernik et al includes 3 some of Burton et al' s summary data on lamina and midrib nitrite and TSNA contents in the KY160 and KY171 samples. Data from the freeze-drying and the quick-drying tests are included, but there is no mention of the microwaved samples. The article contains the following conclusion: It can be concluded from this study that it may be possible to reduce nitrite levels and accurau] ation of TSNA in lamina and midrib by applying heat (70°C) to dark tobacco after loss of cell integrity in the leaf. Drying the tobacco leaf quickly at this stage of curing reduces the microbial activity that occurs during slow curing at ambient temperature. It must be added, however, that such a treatment lowers the quality of the tobacco leaf. Id. at page 5 6. The Weirnik et al article also discusses traditional curing of Skroniowski tobacco in Poland as an example of a 2-step curing procedure. The article states that the tobacco is first air-cured and, when the lamina is yellow or brownish, the tobacco is heated to 65°C for two days in order to cure the stem. An analysis of tobacco produced in this manner showed that both the nitrite and the TSNA values were low, i.e., less than 10 micrograms per gram and 0.6-2.1 micrograms per grams, respectively. Weirnik et al theorized that these results were explainable due to the rapid heating which does not allow further bacterial growth. Weirnik et al also noted, however, that low nitrite and TSNA values, less than 15 micrograms per gram of nitrite and 0.2 microgram per gram of TSNA, were obtained for tobacco subjected to air-curing in Poland. 4 Summary of the Invention One object of the present invention is to substantially eliminate or reduce the content of nitrosammes in tobacco intended for smoking or consumption by other means. Another object of the present invention is to reduce the carcinogenic potential of tobacco products, including cigarettes, cigars, chewing tobacco, snuff and tobacco-containing gum and lozenges. Still another object of the present invention is to substantial]y eliminate or significantly reduce the amount of tobacco-specific nitrosamines, including N'-nitrosonornicotine (NNN) , 4 - (N-mtrosomethylammo) 1 (3-pyridyl) - 1 - butanone (NNK) , N' -nitrosoanatabme (NAT) and N' -mtrosoanabasme (NAB) , in such tobacco products Another object of the present invention is to treat uncured tobacco at an appropriate time post-harvest so as to arrest the curing process without adversely affecting the tobacco's suitability for human consumption. Another object of the present invention is to reduce the content of tobacco-specific nitrosammes in fully cured tobacco. Accordingly, the present invention provides a method of producing tobacco having low levels of nitrosammes, comprising: subjecting a harvested tobacco leaf, to microwave energy, while said leaf is uncured, yellow, and in a state susceptible to having the formation of nitrosammes arrested, for a sufficient time to substantially prevent formation of nitrosamme. 5 It is preferred that in the process of the invention, the step of subjecting to microwave radiation is carried out on a tobacco leaf or portion thereof after onset of yellowing in the leaf and prior to substantial accumulation of tobacco-specific nitrosammes in the leaf. It is also preferred that in the process of the invention, the step of subjecting to microwave radiation is carried out prior to substantial loss of the leaf's cellular integrity. In additional preferred embodiments of the process, the tobacco is flue tobacco and the step of subjecting to microwave radiation is carried out within about 24 to about 72 hours post-harvest, even more preferably within about 24 to about 36 hours post-harvest. In still other embodiments of the process, the harvested tobacco is maintained under above-ambient temperature conditions in a controlled environment prior to the step of subjecting to microwave radiation. Preferred aspects of the process include a step, prior to subjecting a tobacco leaf which preferably includes the stem to microwave radiation, of physically pressing the leaf to squeeze excess moisture therefrom, to ensure more uniform drying by the microwave unit. This step can be conveniently carried out by 6 passing the leaf through a pair of appropriately spaced rotating cylindrical rollers prior to entering the microwave cavity. In yet additional preferred embodiments of the invention, the microwave radiation has a frequency of about 900 to about 2500 MHz, and is applied to the plant for a period of at least about 1 second, and preferably from about 10 seconds to about 5 minutes at a predetermined power level. The power level used generally determines the length of time to which the tobacco is subjected to the microwave radiation, and can range from about 600 to about 1000 watts when using conventional kitchen-type microwave ovens, up to several hundred or more kilowatts for commercial, multimode applicators. Preferred power levels using applicators designed to handle single leaves range from about 2 to about 75 kilowatts, more preferably from about 5 to about 50 kilowatts, which permit relatively rapid treatment to be carried out. It is also preferred in accordance with the present invention that the microwave radiation is applied to the leaf or portion thereof for a time sufficient to effectively dry the leaf, without charring, so that it is suitable for human consumption. The present invention also seeks to subject tobacco leaves to microwave radiation to prevent normal accumulation of at least one tobacco-specific nitrosamine, such as N'-nitrosonornicotine, 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone, N*-nitrosoanatabine and N'-nitrosoanabasine. The present invention in its broadest forms also encompasses a tobacco product comprising non-green tobacco suitable for human 7 consumption and having a lower content of at least one tobacco-specific nitrosamine than conventionally cured tobacco. In preferred embodiments, the non-green tobacco product has a TSNA (NNN, NNK, NAB and NAT) content of less than .2 µg/g, more preferably less than about ,15 µg/g, and even more preferably less than about .1 µg/g, an NNN content of less than about .15 µg/g, more preferably less than about .10 µg/g, and even more preferably less than about .05 µg/g, and an NNK content of less than about .002 µq/g, more preferably less than about .001 µg/g, and even more preferably, less than about .0005 µg/g. The present invention is also directed to a tobacco product comprising dried yellow tobacco suitable for human consumption and having a lower content of at least one tobacco-specific nitrosamine than conventionally cured tobacco. In preferred embodiments, the yellow tobacco product has a TSNA (NNN, NNK, NAB and NAT) content, an NNN content, and an NNK content within the above preferred ranges. In other embodiments, the non-green or yellow tobacco product comprises non-green or yellow tobacco suitable for human consumption, and having a TSNA (NNN, NNK, NAB and NAT) content within about 25% by weight of the content of such TSNA in the freshly harvested green tobacco crop from which the product was made. It is more preferred that the non-green or yellow tobacco product have a TSNA content within about 10% by weight, more preferably within about 5% by weight and most preferably essentially approximating (e.g. within an amount up to several 8 percent by weight) the content of such TSNA in the freshly harvested tobacco crop from which the product was made. It is also preferred that the non-green or yellow tobacco product comprises non-green or yellow tobacco suitable for human consumption, and having content of at least one TSNA selected from NNN, NNK, NAB and NAT, which is within about 25% by weight, preferably within about 10% by weight, more preferably within about 5% by weight and most preferably essentially approximating (e.g. within an amount up to several percent by weight) of the content of the corresponding TSNA or TSNAs in the freshly harvested green tobacco crop from which the product was made. In yet additional embodiments of the invention, the non-green or yellow tobacco product comprises non-green or yellow tobacco suitable for human consumption, and having a TSNA (NNN, NNK, NAB and NAT) content which is at least about 75% by weight, preferably at least about 90% by weight, more preferably at least about 95% by weight, and most preferably at least about 99% by weight lower than the content of such TSNA in a tobacco product of the same type made from the same tobacco crop as the product of the invention, but which was cured in the absence of microwave radiation or other techniques designed to reduce TSNA content. It is also preferred that the non-green or yellow tobacco product comprises non-green or yellow tobacco suitable for human consumption, and having a content of at least one TSNA selected from NNN, NNK, NAB and NAT which is at least about 75% by weight, preferably at least about 90% by weight, more preferably at least about 95% by weight, and most 9 preferably at least about 99% by weight lower than the content of the corresponding TSNA or TSNAs in a tobacco product of the same type made from the same tobacco crop as the product of the invention, but which was cured in the absence of microwave radiation or other techniques designed to reduce TSNA content. A preferred form of the present invention relates to a tobacco product comprising tobacco having a reduced content of at least one tobacco-specific nitrosamine, produced by a process comprising subjecting the tobacco, while the tobacco is uncured and susceptible to having formation of at least one tobacco-specific nitrosamine arrested, to microwave radiation. In another embodiment, the present invention is directed to a method for reducing the content of at least one tobacco-specific nitrosamine in cured brown tobacco, comprising rehydrating the cured brown tobacco, and subjecting the rehydrated tobacco to microwave radiation at a predetermined energy level for a predetermined length of time. Similarly, the present invention includes within its scope a tobacco product comprising cured brown tobacco having a reduced content of at least one tobacco-specific nitrosamine, produced by a process comprising rehydrating the cared brown tobacco, and subjecting the rehydrated tobacco to microwave radiation at a predetermined energy level for a predetermined length of time. In yet another embodiment, the present invention relates to a method of manufacturing a tobacco product, comprising 10 subjecting harvested tobacco leaves to microwave radiation, while said leaves are uncured and in a state susceptible to having the amount of tobacco-specific nitrosamines reduced or formation of tobacco-specific nitrosamines arrested, for a sufficient time to reduce the amount of or substantially prevent formation of at least one tobacco-specific nitrosamine in the leaves, and forming the tobacco product comprising the microwaved leaves, the tobacco product being selected from cigarettes, cigars, chewing tobacco, snuff and tobacco-containing gum and lozenges. Brief Description of the accompanying drawinqs FIG. 1 is a photograph illustrating "yellow" Virginia flue tobacco aged 24 to 72 hours post-harvest. FIG. 2 is a photograph illustrating low-nitrosamine microwaved "yellow" Virginia flue tobacco in accordance with the present invention. Detailed Description Of The Invention It has been said that the practice of tobacco curing is more of an art than a science, because curing conditions during any given cure must be adjusted to take into account such factors as varietal differences, differences in leaves harvested from various stalk positions, differences among curing barns where used, and environmental variations during a single season or over different seasons, especially Weather fluctuations when air-curing. For example, the practice of flue curing is empirical to a certain 11 degree, and is optimally carried out by individuals who "-have accumulated experience in this art over a significant period of time. See, e.g., Peele et al, "Chemical and Biochemical Changes During The Flue Curing Of Tobacco," Recent Advances In Tobacco Science, Vol. 2L, pp. 81 et seq., Symposium Proceedings 49th Meeting Chemists' Research Conference, September 24-27, 1995, Lexington, Kentucky (hereinafter "Peele et al"). Thus, one of ordinary skill in the art of tobacco curing would understand that the outer parameters of the present invention, in its broadest forms, are, variable to a certain extent depending on the precise confluence of the above factors for any given harvest. In one preferred embodiment, the present invention is founded on the discovery that a window exists during the tobacco curing cycle, in which the tobacco can be treated in a manner that will essentially prevent the formation of TSNA. Of course, the precise window during which TSNA formation can be effectively eliminated or substantially reduced depends on the type of tobacco, method of curing, and a number of other variables, including those mentioned above. In accordance with this preferred embodiment of the present invention, the window corresponds to the time frame post-harvest when the leaf is beyond the fresh-cut or "green" stage, and prior to the time at which TSNAs and/or nitrites substantially accumulate in the leaf; this time frame typically corresponds to the period in which the leaf is undergoing the yellowing process or is in the yellow phase, before the leaf begins to turn brown, and prior to the substantial loss of cellular integrity. Unless otherwise clear 12 from the context, the terms "substantial" and "significant" as used herein generally refer to predominant or majority on a relative scale, give or take. During this time frame, the leaves are susceptible to having the formation of TSNAs substantially prevented, or the content of any already formed TSNAs reduced, by exposing the tobacco to microwave radiation at a predetermined energy level for a predetermined length of time, as discussed further below. This microwave treatment essentially arrests the natural formation of TSNAs, and provides a dried, golden yellow leaf suitable for human consumption. If TSNAs have already begun to substantially accumulate, typically toward the end of the yellow phase, the application of microwave energy to the leaf in accordance with the invention effectively arrests the natural TSNA formation cycle, thus preventing any further substantial formation of TSNA. When yellow or yellowing tobacco is treated in this fashion at the most optimal time in the curing cycle, the resulting tobacco product has TSNA levels essentially approximating those of freshly harvested green tobacco, while maintaining its flavor and taste. In another embodiment, the present invention relates to treatment of cured (brown) tobacco to effectively reduce the TSNA content of that cured tobacco, by rehydrating cured tobacco and subjecting the rehydrated cured tobacco to microwave radiation, as described further below. The present invention is applicable to treatment of the harvested tobacco which is intended for human consumption. Much 13 research has been performed on tobacco, with particular reference to tobacco-specific nitrosamines. Freshly harvested tobacco leaves are called "green tobacco" and contain no known carcinogens, but green tobacco is not suitable for human consumption. The process of curing green tobacco depends on the type of tobacco harvested. For example, Virginia flue (bright) tobacco is typically flue-cured, whereas Burley and certain dark strains are usually air-cured. The flue-curing of tobacco typically takes place over a period of five to seven days compared to one to two+ months for air-curing. According to Peele et al, flue-curing has generally been divided into three stages: yellowing (35-40°C) for about 36-72 hours (although others report that yellowing begins sooner than 36 hours, e.g., at about 24 hours for certain Virginia flue strains), leaf drying (40-57°C) for 48 hours, and midrib (stem) drying (57-75°C) for 4 8 hours. Many major chemical and biochemical changes begin during the yellowing stage and continue through the early phases of leaf drying. In a typical flue-curing process, the yellowing stage is carried out in a barn. During this phase the green leaves gradually lose color due to chlorophyll degradation, with the corresponding appearance of the yellow carotenoid pigments. According to the review by Peele et al, the yellowing stage of flue-curing tobacco is accomplished by closing external air vents in the barn, and holding the temperature at approximately 35°-37°C This process utilizes a controlled environment, maintains the relative humidity in the barn at approximately 85%, limits moisture 14 lo_ss from the leaves, and allows the leaf to continue the metaholic processes begun in the field. The operator constantly monitors the progress of the cure, primarily by observing the loss of chlorophyll and green color from the leaves, and the development of the desired lemon to golden orange leaf color. With one particular variety of Virginia flue tobacco on which testing has been carried out as described herein, freshly harvested green tobacco is placed in a barn for about 24-48 hours at about 100-110°F until the leaves turn more or less completely yellow (see Figure 1) . The yellow tobacco has a reduced moisture content, i.e., from about 90 weight % when green, versus about 70-40 weight % when yellow. At this stage, the yellow tobacco contains essentially no known carcinogens, and the TSNA content is essentially the same as in the fresh-cut green tobacco. This Virginia flue tobacco typically remains in the yellow stage for about 6-7 days, after which time the leaves turn from yellow to brown. The brown Virginia flue tobacco typically has a moisture content of about 11 to about 15 weight percent. The conversion of the tobacco from yellow to brown results in formation and substantial accumulation of nitrosamines, and an increased microbial content. The exact mechanism by which tobacco-specific nitrosamines are formed is not clear, but is believed to be enhanced by microbial activity, involving microbial nitrate reductases in the generation of nitrite during the curing process. Tobacco-specific nitrosamines are believed to be formed upon reaction of amines with nitrite-derived nitrosating species, such 15 as N02, N203 and N204 under acidic conditions. Weirnik et al discuss the postulated formation of TSNAs at pp. 43-45; a brief synopsis is set forth below. Tobacco leaves contain an abundance of amines in the form of amino acids, proteins, and alkaloids. The tertiary amine nicotine (referenced as (1) in the diagram below) is the major alkaloid in tobacco, while other nicotine-type alkaloids are the secondary amines nornicotine (2), anatabine (3) and anabasine (4). Tobacco also generally contains up to 5% of nitrate and traces of nitrite. Nitrosation of nornicotine (2), anatabine (3), and anabasine (4) gives the corresponding nitrosamines: N'-nitrosonornicotine (NNN, 5), N'-nitrosoanatabine (NAT, 6), and N'-nitrosonabasine (NAB, 7). Nitrosation of nicotine (1) in aqueous solution affords a mixture of 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK, 8) (NNN, 5) and 4 -(N-nitrosomethylamino)-4-(3-pyridyl)-1-butanal (NNA, 9) . Less commonly encountered TSNAs include NNAL (4-N-nitrosomethylamino)-l-(3-pyridyl)-l-buta'nol, 10), iso-NNAL (4-N-nitrosomethylamino)-4-(3-pyridyl)-l-butanol, 11) and iso-NNAC (4-(N-nitrosomethylamino)-4-(3-pyridyl)-butanoic acid, 12). The formation of these TSNAs from the corresponding tobacco alkaloids is shown schematically below, using the designations 1-12 above (reproduced from Weirnik et al, supra, p. 44): 16 It is now generally agreed that green, freshly harvested tobacco contains virtually no nitrite or TSNA, and that these compounds are generated during curing and storage of tobacco. Studies have been made during the past decade to try to determine the events related to the formation of TSNA during curing of tobacco, and several factors of importance have been identified. These include plant genotype, plant maturity at harvest, curing conditions and microbial activity. Studies have shown-that nitrite and TSNA accumulate on air-curing at the time intervals starting after the end of yellowing and ending when the leaf turns completely brown, e.g., 2-3 weeks 17 after harvest for certain air-cured strains, and approximately a week or so after harvest in flue-cured varieties. This is the time during which loss of cellular integrity occurs, due to moisture loss and leakage of the content of cells into the intercellular spaces. Therefore, there is a short window in time during air-curing when the cells have disintegrated, making the nutrition available for microorganisms. Weirnik et al have suggested that nitrite may then substantially accumulate as a result of dissimilatory nitrate reduction, thus rendering formation of TSNA possible. There are a few published reports on the effects of microbial flora on the tobacco leaf during growth and curing and on cured tobacco, as cited in Weirnik et al. However, the involvement of microbial nitrite reductases in the generation of nitrate during curing is presumed. When cell structure is broken down after the yellow phase, and nutrients are made accessible to invading microorganisms, these may produce nitrite under favorable conditions, i.e., high humidity, optimal temperature and anoxia. There is normally a rather short "window" in time when the water activity is still sufficiently high, and the cell structure has disintegrated. In accordance with the present invention, the formation of TSNAs in tobacco is substantially prevented or arrested by subjecting the harvested leaves to microwave radiation under the conditions described herein. In one preferred embodiment, the tobacco leaves are exposed to the microwave energy at a time 18 between the onset of yellowing and the substantial loss of cellular integrity. For optimal results, it is preferred to pass the harvested leaves through the microwave field as single leaves, as opposed to stacks or piles of leaves. Treating the leaves in this manner has been determined to completely or substantially prevent the formation of tobacco-specific nitrosaraines, including the known carcinogens NNN and NNK. In accordance with preferred embodiments of the present invention, non-green and/or yellow tobacco products can be obtained which are suitable for human consumption, and which have a lower content of at least one tobacco-specific nitrosamine than conventionally cured tobacco. Green or fresh-cut tobacco is generally unsuitable for human consumption as noted above; "non-green" as used herein means means the tobacco has at least lost the majority of chlorophyll, and includes without limitation partially yellow leaves, full yellow leaves, and leaves which have begun to turn brown in places. In preferred embodiments, the non-green tobacco product has a TSNA {NNN, NNK, NAB and NAT) content of less than .2 µg/g, more preferably less than about .15 µg/g, and even more preferably less than about .1 µg/g, an NNN content of less than about .15 µg/g, more preferably less than about .10 µg/g, and even more preferably less than about .05 µglg, and an NNK content of less than about .002 µg/g, more preferably less than about .001 µg/g, and even more preferably less than about .000 5 µg/g.As noted above, given the number of factors which can influence TSNA formation in tobacco, one of ordinary skill in the art would 19 understand that these numbers are not absolute, but rather preferred ranges. The present invention is also directed to a tobacco product comprising dried yellow tobacco suitable for human consumption and having a lower content of at least one tobacco-specific nitrosamine than conventionally cured tobacco. in preferred embodiments, the yellow tobacco product has a TSNA (NNN, NNK, NAB and NAT) content, an NNN content, and an NNK content within the above preferred ranges. In other embodiments, the non-green or yellow tobacco product comprises non-green or yellow tobacco suitable for human consumption, and having a TSNA (NNN, NNK, NAB and NAT) content within about 25% by weight of the content of such TSNA in the freshly harvested green tobacco crop from which the product was made. It is more preferred that the non-green or yellow tobacco product have a TSNA content within about 10% by weight, more preferably within about 5% by weight and most preferably essentially approximating (e.g. within an amount up to several percent by weight) the content of such TSNA in the freshly harvested tobacco crop from which the product was made. For example, the present invention permits tobacco products to be made which have a TSNA content within the above-described ranges as to amounts, whereas normally cured tobacco from the same crop would typically generate many times the amount of TSNA in the fresh-cut tobacco. The present invention can effectively lock in the low amounts of nitrosamines found in fresh-cut green tobacco. It is 20 also preferred that the non-green or yellow tobacco product comprises non-green or yellow tobacco suitable for human consumption, and having content of at least one TSNA selected from NNN, NNK, NAB and NAT, which is within about 25% by weight of, preferably within about 10% by weight of, more preferably within about 5% by weight of, and most preferably essentially approximating (e.g., within an amount up to several percent by weight) the conterit of the corresponding TSNA or TSNAs in the freshly harvested green tobacco crop from which the product was made. In other words, the content of, e.g., NNN in the tobacco of the invention falls within the above ranges vis-a-vis the amount of NNN in the fresh-cut green tobacco, or the amount of NNN + NNK in the tobacco of the invention falls within the above ranges vis-avis the amount of NNN + NNK in the fresh-cut green tobacco, etc. In making these comparisons, the fresh-cut green tobacco is preferably analyzed for TSNA content within about 24 hours after harvest. In yet additional embodiments of the invention, the non-green or yellow tobacco product comprises non-green or yellow tobacco suitable for human consumption, and having a TSNA (NNN, NNK, NAB and NAT) content which is at least about 75% by weight, preferably at least about 90% by we'ight, more preferably at least about 95% by weight, and most preferably at least about 99% by weight lower than the content of such TSNA in a tobacco product of the same type made from the same tobacco crop as the product of the invention, but which was cured in the absence of microwave radiation or other 21 steps specifically designed to reduce the TSNA content. It is also preferred that the non-green or yellow tobacco product comprises non-green or yellow tobacco suitable for human consumption, and having a content of at least one TSNA selected from NNN, NNK, NAB and NAT which is at least about 75% by weight, preferably at least about 90% by weight, more preferably at least about 95% by weight, and most preferably at least about 99% by weight lower than the content of the corresponding TSNA or TSNAs in a tobacco product of the same type (e.g., comparing a cigarette to another cigarette) made from the same tobacco crop as the product of the invention, but which was cured in the absence of microwave radiation or other techniques for reducing TSNA content. In these embodiments, the TSNA weight % comparisons can be made by taking, for example, a cigarette made using dried yellow tobacco in accordance with the present invention, and taking a cigarette made from tobacco from the same crop as the dried yellow tobacco was made from, but curing it by conventional means without subjecting it to microwave radiation. The yellow stage, in which the step of subjecting the tobacco leaf to microwave radiation is preferably carried out, can be broadly defined in any one of the following ways: (a) by examining the color of the leaf, when the green color has substantially given way to a yellowish color; (6) by measuring the percent of chlorophyll conversion to sugars; (c) by observing the onset of either nitrite formation or nitrosamine generation, which typically coincide with the end of the yellow phase, or (d) by measuring the 22 moisture content of the leaves, e.g., when they have a moisture content from about 40 to about 70 percent by weight. If the microwave radiation is applied to green tobacco, the arrestation or prevention of nitrosamine formation is not observed. However, when microwave energy is applied after the onset of yellowing and prior to the loss of cellular integrity or substantial accumulation of TSNAs in the leaf, the observed reduction in the amount of, or prevention of formation of nitrosamines is dramatic and unexpected, as shown by the data discussed below. The optimal time for subjecting the harvested tobacco to the microwave radiation during the yellow phase varies depending on a number of factors, including varietal differences, environmental variations, etc. Thus, within the time frame beginning with onset of yellowing (defined, e.g., by a loss of the majority of green color in the leaf) through the time at which the leaf substantially loses cellular integrity (as it turns brown), one of ordinary skill in the art could determine the optimal time for carrying out the microwave treatment for any given variety of tobacco. For example, for a given genotype, sample leaves could be tested by the procedures described herein to measure either nitrite or TSNA content, to identify the relative time in a given cure cycle at which significant TSNA accumulation begins, or identify the transition phase in which loss of cellular integrity occurs. While subjecting the leaves to the microwave radiation prior to significant TSNA accumulation is the most preferred form of the method of the present invention, the principles of the invention 23 can also be applied to tobacco leaves which are in the process of forming, and have already accumulated significant amounts of TSNAs. When the microwaving is carried out at this latter stage, further formation of TSNAs can be effectively arrested. However, once the leaves are fully cured, TSNA levels have essentially stabilized, and application of microwave radiation is ineffective to reduce the TSNA context, except under rehydration conditions described below. Upon being subjected to microwave radiation in accordance with the present invention, the tobacco leaf generally has a reduced moisture content, i.e. less than about 10% by weight, and often approximately 5%. If desired, the leaf can be rehydrated back to the typical moisture range for brown, cured tobacco (e.g., about 11-15% for Virginia flue) before manufacturing into tobacco products such as cigarettes. The present invention is applicable to all strains of tobacco, including flue or bright varieties, Burley varieties, dark varieties, oriental/Turkish varieties, etc. Within the guidelines set forth herein, one of ordinary skill in the art could determine the most efficient time in the cure cycle for carrying out the microwave step to achieve the objects and advantages of the present invention. Preferred aspects of the process include a step, prior to subjecting a tobacco leaf which preferably includes the stem to microwave radiation, of physically pressing the leaf to squeeze excess moisture therefrom, to ensure more uniform drying by the microwave unit. This step can be conveniently carried out by 24 passing the leaf through a pair of appropriately spaced rotating cylindrical rollers prior to entering the microwave cavity. Such a pressing step will aid in wringing moisture from the stem and, to a lesser extent, the midrib and larger veins, and lead to a better and more evenly dried product. The rollers can be made of hard rubber, plastic or steel and be of any desired length, and are preferably spaced about one-eighth to about one-quarter inch apart, but the distance is preferably selected so as to accomodate the thickness of a single leaf, which can vary. The rollers can be belt or chain driven by an appropriately selected motor. Besides rotating rollers, other types of squeezing or pressing means could be used to accomplish the same result, if desired, as would be apparent to one of ordinary skill in the art. The above-described preferred embodiment of pressing the leaves permits more high-speed production to be carried out, since the stems do not have to be cut out, and the microwave time can be reduced. This embodiment is particularly advantageous for tobacco leaves destined to be used in cigarettes, which typically contain some tobacco stems as part of a blend. Alternatively, the pressing step can be omitted if desired, in applications where the stem is trimmed from the leaves and discarded. The principles of the present invention can also be applied to brown or already cured tobacco, which has been rehydrated. In such cases, while important and unexpected reductions in the amount of the TSNAs, particularly NNN and NNK, are observed when rehydrated brown tobacco is subjected to microwave radiation, the results are 25 not as dramatic as when the invention is applied to uncured yellow tobacco, prior to the time when substantial quantities of TSNAs or nitrites have accumulated in the leaves. Nonetheless, the addition of moisture to the cured leaves, such as by spraying with enough water to effectively soak the leaves, followed by microwaving the rehydrated leaves, reduces the content of TSNAs as demonstrated in the following Examples. As noted above, when treating cured or brown tobacco, microwaving alone has little effect on the nitrosamine content. However, it has been determined that rehydration of the cured tobacco prior to subjecting it to microwave radiation facilitates the action of the microwave energy in reducing nitrosamines. In one preferred embodiment, the cured tobacco product is rehydrated by adding an appropriate amount of water, generally at least about 10% by weight, up to the maximum absorption capacity, directly to the leaves. Exposure of the rehydrated leaves to microwave radiation, in the same manner as described herein with regard to the uncured tobacco, reduces the nitrosamine content, as shown below. The leaves can be wetted in any suitable fashion. If the cured tobacco is in a form other than leaves, such as reconstituted "sheet" tobacco, it can similarly be rehydrated with, e.g., 10-70% by weight water, and then microwaved. Suitable microwave condition can be selected depending on the degree to which the leaves are re-wetted, but typically fall within the parameters discussed above for microwaving yellow tobacco. 26 In accordance with the present invention, microwaving of the rehydrated brown tobacco can preferably reduce the TSNA (NNN, NNK, NAB and NAT) content, measured individually or collectively, by at least about 25% by weight, more preferably by at least about 35% by weight, and even more preferably by at least about 50% by weight from the TSNA levels contained the cured brown tobacco prior to rehydration. The term "microwave radiation" as used herein refers to electromagnetic energy in the form of microwaves having a frequency and wavelength typically characterized as failing within the microwave domain. The term "microwave" generally refers to that portion of the electromagnetic spectrum which lies between the far-infrared region and the conventional radiofrequency spectrum. The range of microwaves extends from a wavelength of approximately 1 millimeter and frequency of about 300,000 MHz to wavelength of 30 centimeters and frequency of slightly less than about 1,000 MHz. The present invention preferab]y utilizes high power applications of microwaves, typically at the lower end of this frequency range. Within this preferred frequency range, there is a fundamental difference between a heating process by microwaves and by a classical way, such as by infrared (for example, in cooking) : due to a greater penetration, microwaves generally heat quickly to a depth several centimeters while heating by infrared is much more superf.icial. In the United States, commercial microwave apparatuses, such as kitchen microwave ovens, are available at standard frequencies of approximately 915 MHz and 2 4 50 MHz, 27 respectively. These frequencies are standard industrial bands. In Europe, microwave frequencies of 2450 and 896 MHz are commonly employed. Under properly balanced conditions, however, microwaves of other frequencies and wavelengths would be useful to achieve the objects and advantages of the present invention. Microwave energy can be generated at a variety of power levels, depending on the desired application. Microwaves are typically produced by magnatrons, at power levels of 600-1000 watts for conventional kitchen-level microwave apparatuses (commonly at about 800 watts), but commercial units are capable of generating power up to several hundred kilowatts, generally by addition of modular sources of about 1 kilowatt. A magnatron can generate either pulsed or continuous waves of suitably high frequency. The applicator (or oven) is a necessary link "between the microwave power generator and the material to be heated. For purposes of the present invention, any desired applicator can be used, so long as it is adapted to permit the tobacco plant parts to be effectively subjected to the radiation. The applicator should be matched to the microwave generator to optimize power transmission, and should avoid leakage of energy towards the outside. Multimode cavities (microwave ovens), the dimensions of which can be larger than several wavelengths if necessary for large samples, are useful. To ensure uniform heating in the leaves, the applicator can be equipped with a mode stirrer (a metallic moving device which modifies the field distribution continuously), and with a moving table surface, such as a conveyor belt. The best 28 results are attained by single leaf thickness exposure to microwave radiation, as opposed to stacks or piles of leaves. In preferred embodiments of the invention, the microwave conditions comprise microwave frequencies of about 900 MHz to about 2500 MHz, more preferably about 915 MHz and about 2450 MHz, power levels of from about 600 watts up to 300 kilowatts, more preferably from about 600 to about 1000 watts for kitchen-type applicators and from about 2 to about 75 kilowatts, more preferably from about 5 to about 5 0 kilowatts, for commercial multimode applicators. The heating time generally ranges from at least about 1 second, and more generally from about 10 seconds up to about 5 minutes. At power levels of about 300-1000 watts the heating time is preferably from about 1 minute to about 21/2 minutes when treating single leaves as opposed to piles or stacks. For commercial-scale applicators using higher power levels in the range of, e.g., 2-75 kilowatts, heating times would be lower, ranging from about 5 seconds up to about 60 seconds, and generally in the 10-30 second range at, say, 50 kilowatts, again for single leaves as opposed to piles or stacks. Of course, one of ordinary skill in the art would understand that an optimal microwave field density could be determined for any given applicator based on the volume of the cavity, the power level employed, and the amount of moisture in the leaves. Generally speaking, use of higher power levels will require less time during which the leaf is subjected to the microwave radiation. 29 However, the above-described conditions are not absolute, and given the teachings of the present invention, one of ordinary skill in the art would be able to determine appropriate microwave parameters. The microwave radiation is preferably applied to the leaf or portion thereof for a time sufficient to effectively dry the leaf, without charring, so that it is suitable for human consumption. It is also preferred to apply the microwave radiation to the leaf or portion thereof for a time and at a power level sufficient to reduce the moisture content to below about 10% by weight. As disclosed in FIG. 2, the microwave treatment of yellow tobacco in accordance with the present invention preferably results in a dried, golden-colored tobacco product. The data presented herein establish that such dried tobacco, in its unsmoked form, has dramatically reduced carcinogenic nitrosamines, particularly NNN and NNK, as opposed to normally cured tobacco. Subjecting the uncured tobacco to microwave energy is demonstrated herein to be effective to provide tobacco have surprisingly low nitrosamine contents. The technique of microwaving can be facilitated by peeling and disposing of the stem down one-third to one-half length of the tobacco leaf, especially in cases where the stem is to be discarded and the moisture-wringing step described above. Where the stem is removed in this manner, the resultant microwaved tobacco leaf does not require the use of a thrasher machine since the undesirable part of the stem is already removed. As a result, the typical loss of tobacco product 30 associated with thrashing is eliminated, reducing tobacco waste by approximately 10% to 30%. The improved tobacco of the present invention can be substituted in whole or part for normally-cured tobacco in any tobacco product, including cigarettes, cigars, chewing tobacco, tobacco chewing gum, tobacco lozenges, tobacco pouches, snuff, or tobacco flavoring and food additives. For the purposes of smoking, the present invention provides a less noxious odor while maintaining good smoking characteristics and providing full 'flavor with normal nicotine content. For the purposes of chewing, snuff, pouch and food additives, the tobacco of the present invention has a rich, pleasant flavor. The present invention is now illustrated by reference to the following examples, which are not intended to limit the scope of the invention in any manner. Example 1 Virginia flue tobacco was harvested, and the leaves were placed in a curing barn at about 100-110°F to begin the flue-curing process. Samples 1-3 were taken from the barn after the leaves had turned yellow, about 24-36 hours post-harvest. Sample 1 was a lamina sample, stripped of the midrib, and baked in a convection air oven at about 400-500°C for about 1 hour, which browned the lamina. Sample 2 was a yellow leaf, placed in a Goldstar Model MA-1572M microwave oven (2450 MHz), and heated on the high power setting (1,000 watts) while rotating for about 21/2 minutes. Sample 31 3 was a yellow leaf, untreated, used as a control. Samples 4 and 5 remained in the curing barn under elevated temperature of about 180°F, Sample 4 being dried outside the racks and Sample 5 inside the racks. Sample 6 was a cured, brown leaf, having underwent the normal flue-cure process. Analyses were performed on each sample to determine NNN, NAT, NAB and NNK contents. In this and the following examples, "TSNA" represents the sum of these four tobacco-specific nitrosamines. Sample work-up and extraction followed a typical procedure for analysis of TSNAs (see, for example, Burton et al., "Distribution of Tobacco Constituents in Tobacco Leaf Tissue. 1. Tobacco-specific Nitrosamines, Nitrate, Nitrite and Alkaloids", J. Agric. Food Chem. , Volume 40, No. 6, 1992) , and individual TSNAs were quantified on a Thermedics Inc. TEA Model 543 thermal energy analyzer coupled to a Hewlett-Packard Model 5890A gas chromatograph. The results are shown in Table 1 below. All data in each table below are presented in micrograms of the nitrosamine per gram of sample (i.e., parts per million or ixg/g) : 32 TABLE 1 Sample # NNN NAT + NAB NNK TSNA 1 - yellow baked lamina 0.0310 0.843 2 - yellow microwaved 3 - yellow control 0.0451 0.1253 0.0356 0.2061 4 - rapid drying outside racks 0.6241 1.4862 1.2248 3 .3351 5 - rapid drying inside racks 0.7465 1.5993 1.3568 3.7044 6 -regularflue-cured 1.0263 1.7107 2.2534 4.9904 Example 2 Virginia flue tobacco was harvested. Sample 7 was a fresh-cut, green leaf used as a control, while Sample 8 was a fresh-cut green leaf which was subjected to microwave radiation in a multimode microwave applicator manufactured by MicroDry of Louisville, Kentucky, operating at 2450 MHz at 2.5 kilowatts, for about 20 seconds. Samples 9-12 were made from normally flue-cured brown tobacco. Sample 9 was tobacco from a formed cigarette; Sample 10 was loose, shredded tobacco for making cigarettes; Samples 11 and 12 were the same as Samples 9 (cigarette) and 10 (loose), respectively, except that each was subjected to the same microwave conditions as Sample 8. TSNA contents were analyzed in 33 the same "manner as in Example 1. The results are shown in Table 2 below: Table 2 Sample # NNN NAT + NAB NNK TSNA 7 - freshleafcontrol 8 - fresh leaf -microwaved 0.029 0.135 0.0004 0. 164 9 -controlcigarette 1,997 3.495 2.735 8.226. 10 -controlloose 2.067 3.742 2.982 8 . 791 11 -cigarettemicrowaved 2.056 3.499 2.804 8.359 12 - loose microwaved 2.139 3.612 2.957 8.707 Example 3 The following cigarette brands shown in Table 3 were purchased at random at various retailers in Lexington, Kentucky, and analyzed for TSNA content using the procedure described in Example 1: Table 3 Sample # Code No. NNN NAT + NAB NNK TSNA 13-Marlboro-king-pc 288292 3.565 4.538 1.099 9.202 14-Marlboro-king-pc 288292 4,146 4.992 1.142 10.279 34 Sample # Code No. NNN NAT + NAB NNK TSNA 15-Marlboro~king-pc 288292 3.580 4.290 1. 106 8.977 16-Marlboro-king-pc 288292 3.849 4.748 1. 130 9.728 17-Marlboro -lights-100's-bx 288192 4.604 5.662 1.223 11.489 18-Marlboro -lights- 100's-pc 288182 3.471 3.859 1.211 8.541 19-Marlboro -lights-100's-pc 288182 3.488 4.136 1.074 8.698 20-Marlboro -lights-100's-pc 288182 3.566 4 . 240 1. 164 8.970 21-Winston-100's-pc 123143 2.311 2 .968 1.329 6. 608 22-Winston-king 123103 2.241 2.850 1.256 6.348 23-Winston-king-bx 125123 2.162 2.831 1.326 6.319 24-winston-king-bx 123123 2.577 3. 130 1.207 6.914 l— ¦" -¦ ' 25-Winston-king-pc 123103 1.988 2.563 1.234 5.786 26-Winston-lights-100's-pc 123133 2. 161 2.706 1.258 6. 124 35 Sample # Code No. NNN NAT + NAB NNK TSNA 27-Winston-lights-100's-pc 123133 2. 189 2.699 1.262 6.150 28-Winston-lights-100's-pc 123133 2.394 3.385 2.330 8. 109 Example 4 Virginia flue tobacco was harvested, and the leaves were placed in a curing barn at about 100-110°F to begin the flue-curing process. After the leaves turned yellow, about 24-36 hours post-harvest, they were taken out of the barn and microwaved in Goldstar Model MA-1572M microwave oven (2450 MHz), high power setting (1000 watts), for about 2*5 minutes while rotating. The leaves were effectively dried by this procedure, although they did not turn brown, but instead retained their golden-yellow color. The leaves were shredded and made into cigarettes. Samples 29-33 were taken from a batch labeled Red Full Flavor, while Samples 34-38 were taken from a batch labeled blue Light. Samples 3 9-4 2 were cigarettes purchased at a health food store, under the brand Natural American Spirit. Samples 29-42 were analyzed for TSNA content using the procedure described in Example 1, and the results are shown in Table 4 below: 36 Table 4 Sample # NNN NAT + NAB NNK TSNA 29-RED FULL 0.138 0.393 FLAVOR REP 1 30-RED FULL 0. 192 0.231 FLAVOR REP 2 31-RED FULL 0.129 0.220 FLAVOR REP 3 32-RED 0.145 0.260 FULL FLAVOR REP 4 33-RED 0.140 0. 293 FULL FLAVOR REP 5 AVG 0.149 0.279 STD 0.022 0.062 0. 0001 0.059 34-BLUE 0.173 0. 162 LIGHT REP 1 35-BLUE 0.046 0.229 LIGHT REP 2 36-BLUE 0.096 0.188 LIGHT REP 3 37-BLUE -0.067 0.215 LIGHT REP 4 38-BLUE 0. 122 0.218 LIGHT REP 5 AVG 0.101 0.202 STD 0.044 0.024 0.0000 0.028 37 Sample # NNN NAT + NAB NNK TSNA NATURALAMERICANSPIRIT 0. 747 1.815 1.455 4 . 017 40-NATURALAMERICANSPIRIT 0.762 1.805 1.458 4.025 41-NATURAL AMERICAN SPIRIT 0.749 1.826 1.464 4.039 42-NATURAL AMERICAN SPIRIT 0.749 1.760 1.462 3.971 AVG 0.752 1.802 1.460 4.013 STD 0.006 0.025 0.004 0. 025 STD in the Tables herein is the standard deviation for the average of the samples shown. Example 5 Virginia flue tobacco was harvested, and the leaves were placed in a curing barn at about 100-110°F to begin the flue-curing process. Samples 43-44 were taken from the barn after the leaves had turned yellow, about 24-36 hours post-harvest, and subjected to microwave radiation in the MicroDry multimode applicator described above for about 20 and 30 seconds, respectively, at a power level of about 6 kilowatts. Samples 43 and 44 were dried, golden-yellow leaves after the microwaving. Samples 45-51 were made from brown, cured leaves having underwent the normal flue-cure process. Sample 38 45 -was a control; Samples 46 and 47 were baked in a convectiomoven preheated to about~400-500 °F for about 1 and about 3 minutes, respectively; and Samples 48 and 49 were subjected to microwave radiation (915 KHz) in a Waveguide applicator Model WR-975, a large multimode oven manufactured by MicroDry (power settings from 0-75 KW) at 5 0 kilowatts for about 10 and 4 0 seconds, respectively. Samples 50 and 51 were cut (reconstituted sheet) tobacco made from the flue-cured leaves. Sample 50 was subjected to microwave radiation in the Waveguide microwave oven at 50 kilowatts for about 1.5 minutes, while Sample 51 was baked in a convection oven preheated to about 400-500 °F for about 3 minutes. These samples were analyzed for TSNA content using the procedure described in Example 1, and the results are shown in Table 5 below: Table 5 Sample # NNN NAT + NAB NNK TSNA 43-20 SEC MICROWAVE 44-30 SEC MICROWAVE 4 5-CONTROL HO MICRO 0.9 2 2.05 3.71 6.68 46-OVEN 1 MIN 1.14 2.41 5.10 8.66 47-OVEN 3 MIN 0.89 2.06 2.68 5. 64 48-WAVEGUIDE 10 SEC 50 KW 1.0 0 2.31 3.29 6.59 39 Sample # NNN NAT + NAB NNK TSNA 49-WAVEGUIDE 40 SEC 50 KW 0.6 2 1.55 1.69 3 . 86 5 0-CUT TOBACCO WAVEGUIDE 1.5 MIN 50 KW 4.22 4.91 0.99 10.12 51-CUT TOBACCO OVPM 3 MIN 4.76 5.60 1.08 11.44 Example 6 Virginia flue tobacco was harvested, and the leaves were placed in a curing bar at about 100-110°F to begin the flue-curing process. Samples 52-55 were cigarettes made from yellow tobacco which had been pulled from the barn after about 24-36 hours, and subjected to Microwave radiation in a Goldstar microwave oven, Model MA-1572M (2450 MHz), for about 2 minutes on the high power setting (1000 watts). For comparison, Samples 61 and 62 were cigarettes made from leaves which had undergone the normal flue-cure process, without microwave treatment. Sample 56 was a cured leaf; Sample 57 was post-yellow, not fully cured; Sample 58 was a cured lamina, while Samples 59 and 60 were cured midribs. TSNA contents were measured as in Example 1, and the results are set forth in Table 6 below: 40 Table 6 Sample # NNN NAT + NAB NNK TSNA 52-Goldsmokecigarettes 0.12 0.23 0.03 0.38 53-Goldsmoke II, 85 mm 0.062 0.326 0.016 0. 404 54-Goldsraoke 85 mm 0. 128 0.348 0.029 0.504 55-Goldsmoke100'sSample B 0.166 0.317 0.047 0.531 , 56-SampleM-M 3.269 4.751 0.833 8.853 57-Sample B-C 0.267 0.720 0.954 1.941 58-Lamina M-C 0.933 1.456 1.960 A .356 59-WM 0.996 1.028 0.408 2.432 60-SM 1.745 1.753 0.306 3.804 61-Goldsmokecontrol 1.954 1.544 0.492 3 .990 62-Goldsmoke control 1.952 1.889 0.424 4.265 Example 7 Virginia flue tobacco was harvested. Samples 63 and 66 were uncured, fresh-cut green tobacco, although over a week lapsed before TSNA measurements were taken, so some air-curing had taken place. The remaining leaves were placed in a curing barn at about 100-110°F to begin the flue-curing process, sample 68 was a leaf 41 taken from the barn after it had turned yellow, about 24-36 hours post-harvest, and was subjected to microwave radiation in the Waveguide multimode applicator described above, for about 40 seconds at 25 kilowatts. Samples 64/65 (leaves) and 67/70 (reconstituted sheet tobacco, or "cut" tobacco) demonstrate the effects of the present invention when cured tobacco is rehydrated, then subjected to microwave radiation. Samples 64 and 65 were leaf samples having undergone the normal flue-curing process; however, Sample 64 was rehydrated by running under an open faucet for about 5-10 seconds. The leaf absorbed significant moisture. Each of Samples 64 and 65 was then microwaved in the Waveguide multimode applicator for about 4 0 seconds at 25 kilowatts. Samples 67 and 70 were reconstituted sheet tobacco samples, made from cured leaves. Sample 67 was rehydrated by adding water so that a significant quantity was absorbed, then microwaved under the conditions described for Sample 6 4 . Sample 7 0 was not microwaved. Samples 69, 71 and 7 2 are additional cured leaf samples, used as controls. The TSNA contents were measured as in Example 1, and the results are shown in Table 7 below: Table 7 Sample # NNN NAT + NAB NNK TSNA 63-CONTROL UNCURED 0.010 0. 263 0.000 0.274 64-CURED 40 SEC (WET) 0.737 1.252 1.893 3.882 42 Sample # NNN NAT + NAB NNK TSNA 65-CURED 40 SEC 0.767 1.520 2. 229 4 . 516 66-UNCURED 40 SEC 0.010 0.261 0.000 0.272 67-CUT TOBACCO CURED 4 0 SEC (WET) 0.769 1.328 0.308 2.405 68-UNCURED 40 SEC 25 KW WAVEGUIDE 0.051 0.244 0.014 0.308 69-CURED CONTROL 0.866 1.548 2.545 4.960 70-CONTROLCUTTOBACCO 1.872 2.536 0.789 5.197 71-CONTROL ' AL' WHOLE LEAF 0.230 0. 606 0.746 1.582 72-SML WHOLE LEAF 0.413 0. 884 1.514 2.810 Example 8 Virginia flue tobacco was harvested, and the leaves were placed in a curing barn at about 100-110° F to begin the flue-curing process. Sample 73 was a leaf taken from the barn after it turned yellow, about 24-36 hours post-harvest, and microwaved in a Goldstar Model MA-1572-M for about 2 minutes on the high setting. Samples 74-76 were flue-cured in the normal way. Sample 74 was a cured control. Samples 75 and 76 were rehydrated as in Example 7 (Sample 64), then each sample was subjected to microwave radiation in the MicroDry applicator (2450 MHz) for about 20 seconds (Sample 43 75) and about 40 seconds (Sample 76), respectively, at power levels of about 6 kilowatts. Samples 77-79 were reconstituted sheet tobacco, made from the flue-cured leaves. Sample 77 was a control, while Samples 78 and 79 were rehydrated as in Example 7 (Sample 67). Samples 78 and 79 were microwaved in the MicroDry applicator for about 30 seconds each; Sample 78 rested on the oven bottom, while Sample 79 was raised up several inches by resting the sheet sample on a styrofoam cup, which permitted more uniform heating. TSNA contents were measured as in Example 1, and the results are set forth in Table 8 below: Table 8 Sample # NNN NAT + NAB NNK TSNA 73-yellow/ microwaved 0.052 0.260 74-A-control leaf, cured 1. 168 1.904 1.662 4.734 75-B- 20 SECONDS 0.791 1.705 1.115 3.611 76-C 40 SECONDS 0.808 1.624 1.160 3.592 77-CONTROL-sheet 4 .417 3.697 0.960 9.073 78-30 SECONDS 2.755 2.553 0.644 5.952 79-30SECONDSELEVATED 1.606 1.732 0.350 3.687 44 Example 9 Samples 80-81 were Redman chewing tobacco purchased at retail. Sample 80 was a control, while Sample 81 was microwaved in a Goldstar Model MA-1572M for about 1-2 minutes on the high power setting. Samples 82-83 were Skoal snuff purchased at retail. Sample 82 was a control, while Sample 83 was microwaved in the same manner as for Sample 81. TSNA contents were measured, and the results are shown in Table 9 below: Table 9 Sample # NNN NAT + NAB NNK TSNA 8 0-CHEWINGTOBACCOBEFORE 0.712 0.927 0.975 1.713 81-CHEWINGTOBACCOAFTER 0 . 856 0.906 0.122 1. 884 82-SNUFF BEFORE 4. 896 10.545 1.973 17.414 83-SNUFF AFTER 6. 8 60 14.610 1.901 23.370 Example 10 To test whether TSNAs accumulate over time even after yellow tobacco is microwaved in accordance with the present invention, additional samples (designated -A) of the cigarettes tested in Example 4, Samples 29, 35 and 39 (control) were retested for TSNA content more than seven months after the TSNA contents were first measured, as reported in Example 4. The results are shown below in Table 10: 45 Table 10 Sample # NNN NAT NAB NNK TSNA 29A-RED FF REP#1 0.1109 0.1877 0.1078 0.0015 0.4079 3 5A-BLUE LIGHT REP #2 0.0508 0.1930 0.1075 0.0012 0.3525 39A-NATURAL AMERICAN SPIRIT REP #1 0.6151 1.2357 0.1072 0.9302 2 . 8882 Example 11 Virginia flue tobacco was harvested, and the leaves were placed in a curing barn at about 100-110°F to begin the flue-curing process. After the leaves turned yellow, about 24-36 hours post-harvest, they were taken from the barn and subjected to microwave radiation in a Goldstar Model MA-1572M microwave oven for about 2 to 1\ minutes, on the high power setting. Each of the leaves was a golden-yellow color, and effectively dried. Certain samples, designated by "ground", were later ground up into a flour-like substance, which would be useful as, for example, a gum, lozenge or food additive. After more than six months from the time the leaves were microwaved, the TSNA content of the following samples were measured using the procedure described in Example 1, The results are shown in Table 11 below: 46 Table 11 Sample # HNN NAT NAB NNK TSNA 84-ground 0.0013 0.0018 0.0018 0.0015 0.0064 85-ground 0.0469 0.0341 0.0011 0.0009 0.0831 86-ground 0.0009 0.0582 0.0013 0.0011 0.0615 87-ground 0.0113 0.1078 0.1078 0.0015 0. 2284 88-ground 0.0569 0.1401 0.1071 0.0009 0.3051 89-ground 0.0109 0.1642 0.1073 0. 0011 0.2835 90-ground 0.0008 0.0011 0.0011 0.0009 0.0038 91-ground 0.0009 0.0012 0.0012 0.0010 0.0044 92-ground 0.0012 0.1059 0.0017 0.0014 0.1101 93-ground 0.0013 0.0529 0.0019 0.0015 0.0576 94-ground 0.0012 0.0613 0.0017 0.0014 0.0657 95-ground 0.0506 0.0989 0.0013 0.0010 0.1518 96-ground 0.0017 0.0894 0.0024 0.0019 0.0954 97-ground 0.0012 0.0017 0.0017 0.0014 0.0061 98-ground 0.0016 0.0023 0.0023 0.0019 0.0082 99-ground 0.0342 0.0016 0.0016 0.0013 0.0386 100-ground 0.0014 0.0020 0.0020 0.0016 0.0070 101-leaf 0.0013 0.0539 47 Sample # NNN NAT NAB NNK TSNA 102-leaf 0.0009 0.0012 103-shreddedleaves 0.0202 0.0327 It will be apparent to those skilled in the art that various changes and modifications may be made in the preferred embodiments without departing from the spirit and scope of the claimed invention. Therefore, the foregoing description is intended to be illustrative only and should not be viewed in a limiting sense. 48 I CLAIM: l.A method of producing tobacco having low levels of nitrosamines, comprising: subjecting a harvested tobacco leaf to microwave energy,)while said leaf is uncured, yellow, and in a state susceptible to having the formation of nitrosamines arrested, for a sufficient time to substantially prevent formation of a nitrosamine. 2.The method as claimed in claim 1, wherein said subjecting to microwave radiation is carried out on a tobacco leaf prior to substantial accumulation of tobacco-specific nitrosamines in the leaf. 3 The method as claimed in claim 1, wherein said subjecting to microwave radiation is carried prior to substantial loss of the leaf's cellular integrity. 4. The method as claimed in claim 2, wherein the tobacco is flue tobacco and said subjecting to microwave radiation is carried out within 24 to 72 hours post-harvest. 5.The method as claimed in claim 4, wherein the harvested tobacco is maintained under above-ambient temperature conditions in a controlled environment prior to said subjecting to microwave radiation. 6.The method as claimed in claim 1, wherein said microwave radiation has a frequency of 900 to 2500 Mhz. 49 7.The method as claimed in claim 1, wherein said microwave radiation is applied to the leaf for a period of one second or more at a predetermined power level 8, The method as claimed in claim 7, wherein said microwave radiation is applied to the leaf for a period of 10 seconds to 5 minutes at a predetermined power level 9.The method as claimed in claim 7, wherein said power level is from 600 watts to 300 kilowatts. 10.The method as claimed in claim 9, wherein said power level is from 2 to 75 kilowatts. 11.The method as claimed in claim 9, wherein said microwave radiation has a frequency of 900 to 2500 Mhz. 12.The method as claimed in claim 2, wherein said microwave radiation is applied to the leaf a time sufficient to effectively dry the leaf, without charring, so that it is suitable for human consumption. 13.The method as claimed in claim 12, wherein said subjecting to microwave radiation prevents normal accumulation of a tobacco-specific nitrosamme in the leaf. 50 14.The method as claimed in claim 13, wherein said tobacco-specific nitrosamine is selected from the group consisting of N-nitrosonornicotine and 4-(N'--nitrosomethylamino) -1- (3-pyridyl) -1- butanone, N-nitrosoanatabine and N-nitrosoanabasine. l5.The method as claimed in claim 2, wherein said subjecting to microwave radiation is carried out on tobacco leaves arranged in single layer thickness, without stacking or piling of the leaves. 16.The method as claimed in claim 15, wherein, prior to said subjecting to microwave radiation, a step of pressing the tobacco leaves to remove excess moisture. 17.The method as claimed in claim 16, wherein said tobacco leaves contain stems. 18.A method of manufacturing a tobacco product having low levels of tobacco-specific nitrosamines, comprising subjecting a harvested tobacco leaf to microwave energy, as claimed in claim 1, and forming said tobacco product comprising the microwaved leaves, the tobacco product being selected from cigarettes, cigars, chewing tobacco, snuff, and tobacco-containing gum and lozenges. 19 The method as claimed in claim 18, wherein the leaves are subject to microwave radiation prior to substantial accumulation of tobacco-specific nitrosamines in the leaves. 51 20 A method of producing tobacco having low levels of mitrosamines, substantially as herein described, particularly with reference to the examples and as illustrated in the accompanying drawings Dated this 23rd day of September, 1997. 52 The present invention discloses a method of producing tobacco having low levels of nitrosamines, comprising: subjecting a harvested tobacco plant ,or portion, thereof,to microwave energy, while said plant is uncured ,yellow, and in a state susceptible to having the formation of nitrosamines arrested, for a sufficient time to substantially prevent formation of nitrosamine

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01758-cal-1997-correspondence.pdf

01758-cal-1997-description(complete).pdf

01758-cal-1997-drawings.pdf

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01758-cal-1997-form-2.pdf

01758-cal-1997-form-3.pdf

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1758-CAL-1997-(02-12-2011)-CORRESPONDENCE.pdf

1758-cal-1997-granted-abstract.pdf

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1758-cal-1997-granted-correspondence.pdf

1758-cal-1997-granted-description (complete).pdf

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1758-cal-1997-granted-examination report.pdf

1758-cal-1997-granted-form 1.pdf

1758-cal-1997-granted-form 2.pdf

1758-cal-1997-granted-form 3.pdf

1758-cal-1997-granted-form 5.pdf

1758-cal-1997-granted-letter patent.pdf

1758-cal-1997-granted-others.pdf

1758-cal-1997-granted-pa.pdf

1758-cal-1997-granted-reply to examination report.pdf

1758-cal-1997-granted-specification.pdf

1758-cal-1997-granted-translated copy of priority document.pdf