Title of Invention

A NOVEL HERBAL ANTI-BLOAT COMPOSITION

Abstract

ABSTRACT A PROCESS FOR THE PREPARATION OF A HERBAL ANTIBLOAT COMPOSITION The present invention comprises taking the distillate of Mentha spicata and adding it to a suitable emulsifier and mixing it gradually to purified water to obtain a emulsified mixture. Separately taking the exudate of Ferula asafoetida and extracting it with a suitable solvent by process of trituration and filtering the resultant to obtain the extract. The preservatives and solubilisers are separately dissolved in purified water in the required quantities to prepare the preservative solution. To obtain the present invention the emulsified mixture thus prepared is added to the extract and mixed thoroughly, to which later the preservative solution is also added and again mixed thoroughly. The resulting composition has potent anti-bloat and anti-flatulent activity.

Full Text

A PROCESS FOR THE PREPARATION OF A HERBAL AN1TBLOAT COMPOSITION Excessive accumulation of gases within the rumen ( bloat, meteorism, hoven) is a major problem of ruminants the world over. Accumulation of gases within the rumen is normally relieved by belching or eructation. Sometimes, the gases cannot escape because they are . imprisoned within the ingesta, the avenue of escape is blocked and or the nonmotile rumen does not aid in eructation. Bloat usually accompanies rumen stasis but it may also occur alone. Severe bloat can lead to rumen stasis from overdistention of the reticulo-ruminal musculature. Recent work indicates that bloating is a failure to expel gas in a normal manner, rather than a problem of excessive gas production. Gases produced in the rumen are primarily carbon dioxide and methane. Small amounts of other gases, including oxygen, may be present, but the ruminal condition is essentially anaerobic. Some of the ruminal gas is absorbed into the blood stream and eliminated through the lungs, but most ruminal gas is expelled by belching or eructation. Plants having potent antifiatulent and antibloat properties were selected and screened as per the Indian Pharmacopoeia, 1996. From results of the preliminary screening the most potent plants were short listed and several prototype combinations were developed. Each of these were further evaluated in - vitro using the above cited method as well as using ruminal liquor, in - vitro and the final composition was finally selected . The present invention ( composition) was arrived at by blending the ingredients in a specific ratio so as to obtain maximum synergistic activity, therefore, the novelty of the invention resides not only in the composition itself, but also in the 1 ne present invention relates to a process for the preparation of a novel anti-bloat herbal composition. Bloat is a common ailment in ruminants and other livestock. It can threaten the life of the animal if left unattended or neglected. It is a matter of major medical and economic concern. A bloat may be either gaseous - in the form of free gas separated from the ingesta, or frothy bloat due to production of stable and persistent foam mixed with the rumen contents followed by non-eructation of the gas. Fatality often results in such cases due to lack of timely effective treatment. Unsystematic, irregular feeding habits and a drastic change from regular kind of diet also leads to flatulent conditions. The stomach feels bloated and distended and results in a " disturbed, restless, uncomfortable" stage. Indigestion and loss of appetite often accompany it. The present invention counteracts flatus by different routes. The ingredients of the composition are so chosen, that they immediately and actively act against froth/foam formation in the gastrointestinal tract. Bloat occurs when gases enter the gastrointestinal tract from three sources, namely swallowed air, gases formed as a result of bacterial action, and gases that diffuse from the blood into the gastrointestinal tract. Most gases in The stomach are generally nitrogen and oxygen. In addition, considerable carbon dioxide often occurs because the reaction between acidic gastric juice and bicarbonate in pancreatic juice is sometimes too rapid for the liberated carbon dioxide to be absorbed. A very large number of domestic animals are usually met with Tympanitis and this situation is prevalent in all seasons. The animals usually affected are the cows and buffaloes. The disease in live stock can affect the nutrition and economy of a family, a village or a nation. Hence there is an important link between the health of live stock and well being of a country. Tympanitis or bloat is an emergency condition wherein sometimes the veterinarian or farmer drills hole in the abdomen to release the gas or froth from the stomach of the animal. If not attended immediately to this condition, the animal may die because of excessive pressure on other vital organs like lungs, heart etc. To meet the needs of the animals and increase the live stock production, a need was felt to develop the present invention comprising a blend of extracts and distillates obtained from certain plants like Mentha spicata and Ferula asqfoelida. A list of most effective plants known for anti-froth, carminative and stomachic activities was prepared and these were then blended in synergistic combinations and ratio and evaluated through clinical trials. These plants were selected after a thorough literature study followed by experiments on individual plants and their extracts under laborites conditions and finally the present invention was arrived. It is found to be effective in conditions like gaseous and frothy bloat. The present invention is highly effective and safe. Very few synthetic products are available for this condition and more over most of these products are not effective in treating the condition effectively and safely. The present invention comprises taking exudate of Ferula asqfoetida, cleaning, drying it in the open and powdering it to a sieve size of 20-30. This powder is then subjected to extraction by means of an appropriate technique like percolation, refluxation, soxhlet extraction. The extract obtained is filtered through a filter fabric to obtain extract. Leaves and dried flowering heads of Mentha spicata are taken, cleaned and extracted by using suitable solvents. The distillate thus obtained is separated from its solvent and then used in this composition.. According to the present invention, the process for preparation of a herbal anti-bloat composition comprises: a) taking the exudate of Ferula asqfoetida, cleaning and drying in an open atmosphere and powdering it to a seive size of 20-30 and extracted with suitable solvents to obtain the extract. b) taking the leaves and dried flowers of Mentha spicata and extracting it with a suitable solvents to obtain the distillate. c) the two concentrated extracts and distillates thus obtained are mixed in the following ratio along with preservatives, emulsifiers and LP. grade water. 1. Distillates from Mentha spicata equivalent to 80-1 OOgm 2. Extracts from Ferula asafoetida 0.3-0.6 gm d). suitable preservatives, emulsifiers are added to the above said extracts and distillates resulting in the desired anti-bloat composition. Mentha spicata: is a herb found distributed all over India belonging to the family Labiatae and is commonly called Pudina. Its leaves are bitter and are used for culinary purpose. The leaves and flowering branch contain an essential oil, which contains an active principle called 'Carvone'. It is widely used as an appetiser, digestive, carminative and stimulant. It removes flatulence. Its chemical structure is given in figure 1 (enclosed in drawing sheet) Ferula asafoetida: the exudate of Ferula asafoetida is commonly used for culinary purposes. . It is a valuable stimulant acting on the organs of circulation and secretion, also a nervine and pulmonary stimulant and powerful anti-spasmodic It is also a carminative, tonic, laxative, diuretic, emmenogogue and anthelmentic. It is recommended in dyspepsia, colic and other gastric affections. It relieves gaseous indigestion. It has been highly recommended for Tympanitis. Ferulic acid is widely distributed in small amount in plants. But it is found in Asafoetida, the milk sap of the roots of Ferula asafoetida (Umbelliferae). It occurs in cis and trans form. The cis form is a yellow oily liquid whereas the trans form gives orthorhombic needles from water, which has a melting point of 1740C. It is soluble in hot water, alcohol and ethylacetate. Its chemical structure is given in figure 2 (enclosed in drawing sheet) It is anti-bacterial, anti-fungal, anti-hepatotoxic, anti-oestrogenic and anti-tumor activities. It stimulates phagocytosis and inhibits platelet aggregation in-vitro. It acts as a serotonin antagonist and has anti-yeast activity. It is used as a food preservative. EXAMPLE 1 a). The present invention comprises taking the distillate of Mentha spicata. The distillates of Mentha spicata is taken in the ratio of 80-100gms and added to the suitable emulsifier in the ratio of 1-4% w/w and mixing it gradually to LP. grade water at 30-35% w/w to obtain the emulsified mixture. b). Taking the exudate of Ferula asafoetida, cleaning it, drying it in shade and powdering it to a sieve size of 20-30 and extracting it with a suitable solvent by a process of trituration and filtering the resulting extract through a filter fabric to obtain an extract. The extract of Ferula asafoetida is taken in the ratio of 0.3-0.6 gms and added to LP. grade water at 30-35% w/w to obtain extract. If The two extracts and distillates thus obtained are mixed in the following ratio along with suitable preservatives and emulsifying agents. 1. Distillates of Mentha spicata equivalent to 80-100 gms 2. Extracts of Ferula asafoetida equivalent to 0.3-0.6 gms Above (a) and (b) mixtures are mixed together thoroughly and added the following preservative solution. Preservative solution contains preservatives methyl paraben sodium, propyl paraben sodium and bronopol(2 brono-2-nitro propane-1-3-diol) in the ratio of 0.1-0.3% w/w, 0.05-0.1% w/w and 0.05-0.1 % w/w respectively dissolved in small amount of LP. grade water. Then it is made up to 100% w/w using I. P. grade water. EXAMPLE 2 a). The present invention comprises taking the distillate of Mentha spicata. The distillates of Mentha spicata is taken in the ratio of 60-80gms and added to the suitable emulsifier in the ratio of 0.5-3% w/w and mixing it gradually to LP. grade water at 20-25% w/w to obtain the emulsified mixture. b). Taking the exudate of Ferula asafoetida, cleaning it, drying it in shade and powdering it to a sieve size of 10-20 and extracting it with a suitable solvent by a process of trituration and filtering the resulting extract through a filter fabric to obtain an extract. The extract of Fenda asafoetida is taken in the ratio of 0.1-0.4 gms and added to LP. grade water at 20-25% w/w to obtain extract. The two extracts and distillates thus obtained are mixed in the following ratio along with suitable preservatives and emulsifying agents. 1. Distillates of Mentha spicata equivalent to 60-80 gms 2. Extracts of Ferula asafoetida equivalent to 0.1-0.4 gms Above (a) and (b) mixtures are mixed together thoroughly and added the following preservative solution. Preservative solution contains preservatives methyl paraben sodium, propyl paraben sodium and bronopol (2 brono-2-nitro propane-1-3-diol) in the ratio of 0.05-0.2% w/w, 0.01-0.05% w/w and 0.01-0.05% w/w respectively dissolved in small amount of LP. grade water. Then it is made up to 100% w/w using I. P. grade water. The present invention is a very potent anti-flatulent, anti-bloat composition, which relieves the suffering animal swiftly from distress, and restores the digestive functions and production. Its mode of action is as follows: a) It breaks the froth by reducing viscosity of ruminal fluid and thus relieves frothy bloat. b) It reduces surface tension in the froth. This is because of low hydrophobic- lipophilic balance (HLB) value of the product. c) Its spasmolytic action alleviates smooth muscle passion precipitated by accumulated gas in the intestinal tract. d) Rumenatoric action of the invention accelerates the rumeno-reticular contractions to help eructation of the gas and also relieves impaction. e) Its antizymotic properties controls excessive fermentation. f) The antacid action of the composition helps overcome acid indigestion and maintains normal ruminal pH. The composition contains potent digestive and carminative ingredients, which help in expulsion of gas from large intestine and rumen thereby relieving flatulent colic. The present invention therefore offers timely and comprehensive treatment for both frothy and gaseous bloat and restores normal digestion and production quickly. We claim: 1. A process for the preparation of a herbal antibloat composition comprising: (a) taking the leaves and dried flowers of Mentha spicata, cleaning them and distillating them by the conventional method using suitable solvents to obtain the distillate, (b) then adding to it a suitable emulsifier (1-4% w/w) and mixing it gradually with LP. grade water to obtain an emulsified mixture, (c) then taking the exudate of Ferula asafoetida, cleaning it, drying it in shade and powdering It to a seive size of 20-30 and extracting it with suitable solvent by conventional process and filtering it to obtain the extract, (d) mixing the said extract and emulsified mixture in the following ratio: 1. Distillate of Mentha spicata equivalent to 80-100 gm 2. Extract of Ferula asafoetida equivalent to 0.3-0.6 gm (e) adding the preservatives in the ratio 0.01-3% w/w and dissolving them in a small amount of LP. grade water (1-10% w/w) and making up the composition to 100% w/w using LP. grade water and stirring it thoroughly to obtain a homogeneous composition. 2. A process for preparation of a herbal anti-bloat composition as claimed in claim-1, wherein the emulsified mixture is prepared by using the distillates of Anethum sowa or Mentha piperita or a mixture there of 3. A process for preparation of a herbal anti-bloat composition as claimed in claim-1, wherein the emulsified mixture is prepared by using different emulsifiers like Tween 20, Tween 40, Tween 80, GMS(Glyceryl monostearate), Cresmer 1000 and Nonic G2000. 4. A process for preparation of a 1 herbal anti-bloat composition as claimed in claim-1, wherein the extract is prepared by using the gum of Gardenia gummifera. 5. A process for preparation of a herbal anti-bloat composition as claimed in claim-1, wherein the said preservatives are methyl paraben, propyl paraben and bronopol(2 brono-2-nitro propane-1-3-diol). 6. A process for preparation of a herbal anti-bloat composition as claimed in claim-1, wherein the ratio of methyl paraben is 0.1 - 0.3 %w/w, propyl paraben is 0.05-0.1 %w/w and bronopol(2 brono-2-nitro propane-1-3-diol) is 0.05-0.1% w/w. I 7. A process for preparation of a herbal anti-bloat composition as claimed in claim-1, wherein the emulsifier used is Tween 20. 8. A process for preparation of a herbal anti-bloat composition as claimed in claim-1, wherein the ratio of emulsifier is 1 - 4% w/w. 9. A process for the preparation of a novel herbal anti-bloat composition as herein before described with reference to the accompanying examples.

Documents:

140-mas-1998 abstract.pdf

140-mas-1998 claims.pdf

140-mas-1998 correspondence others.pdf

140-mas-1998 correspondence po.pdf

140-mas-1998 description (complete).pdf

140-mas-1998 form-1.pdf

140-mas-1998 form-13.pdf

140-mas-1998 form-26.pdf

140-mas-1998 form-4.pdf

140-mas-1998 form-5.pdf

140-mas-1998 form-6.pdf